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Citations for Miltenyi Biotec :
1251 - 1300 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... with an overnight recovery followed by cryopreservation in 45% TexMACS media (Miltenyi Biotec), 45% heat-inactivated human serum (Seralab) ...
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bioRxiv - Immunology 2024Quote: ... media with 3% heat-inactivated human serum (Seralab, Sussex, UK) and 20 ng/mL human recombinant IL-2 (Miltenyi Biotec). BE-CAR7 products and CART19 control cells were made as previously described using pCCL-CAR7 or pTT-CAR19 vector ...
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bioRxiv - Immunology 2024Quote: ... and cultured at a density of 1×106 per mL in TexMACS (Miltenyi Biotec) media with 3% heat-inactivated human serum (Seralab ...
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bioRxiv - Immunology 2024Quote: ... and (iii) anti-NKG2A-VioBright FITC (clone REA110) from Miltenyi Biotec. Cytosolic/intranuclear expression of Ki67 was detected using anti-Ki67–BV421 (clone B56 ...
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bioRxiv - Immunology 2024Quote: ... or MACS anti-REA Comp Bead kit (Miltenyi Biotec). Data were acquired using an Attune NxT flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The cell pellet was then resuspended in MACS buffer and separated using magnetic-activated cell sorting columns according to the manufacturer’s instructions (Miltenyi Biotec). Single-cell suspensions were collected and B cell numbers and purity (CD19+ ...
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bioRxiv - Immunology 2024Quote: ... via immunomagnetic isolation (Miltenyi Biotec). Extended experimental details are reported in the supplementary methods.
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bioRxiv - Immunology 2024Quote: ... human (Miltenyi Biotec). Cells were frozen and kept in nitrogen until DNA extraction and sequencing as stated below.
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bioRxiv - Immunology 2024Quote: ... The sections were washed again and blocked using 1% Fc block (Miltenyi Biotec) and 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... and 1% Fc block (Miltenyi Biotec) in FACS buffer were added for 15 minutes (4°C) ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25+ Tregs and CD4+CD25− T cells were purified from the peripheral LNs and spleen of Apoe−/− or Ccr4−/−Apoe−/− mice using a CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) and anti-CD4 beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... The production of several major cytokines from CD4+ T cells was examined as described previously.30 Splenic CD4+ T cells (1×105 cells) isolated using MACS (Miltenyi Biotec) were stimulated with plate-bound anti-CD3 (10 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... CD11c+ DCs were isolated from spleen of Apoe−/− mice treated with Liberase TM using MACS (Miltenyi Biotec). The purity of the CD11c+ population was approximately 93% ...
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bioRxiv - Immunology 2024Quote: ... and anti-CD4 beads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1 µg/ml PepTivator SARS-CoV-2 Prot_S Complete peptide pool (Miltenyi Biotec), or an equivalent volume of DMSO (or sterile water as indicated ...
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bioRxiv - Immunology 2024Quote: ... and anti-PE microbeads (Miltenyi Biotec) as per manufacturer’s protocol were seeded at 1-2e6 cells per well ...
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bioRxiv - Immunology 2024Quote: ... Mouse and Rat (Miltenyi Biotec, Gaithersburg, MD; Cat No. 130-107-677) per manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... and subjected them to anti-mouse CD45 bead-based separation (Miltenyi Biotec; Cat. No 130-052-301) per manufactural protocol ...
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bioRxiv - Immunology 2024Quote: ... PBMCs cells were then resuspended by auto MACS buffer and incubated with magnetic microbeads (Miltenyi Biotec) for collecting CD3+ T cells ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched for using the Miltenyi Mouse Naïve CD4+ T cell Purification Kit (Miltenyi Biotec, Cat. # 130-104-453) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions from lymph nodes and spleens were magnetically enriched for CD11c+ cells using CD11c microbeads and MACS separation columns according to the manufacturer’s recommendations (Miltenyi Biotec). Purified splenic DCs were then pulsed with peptides at a final concentration of 15 μg/mL/peptide for 30 minutes at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... anti-CD4-APC-Vio770 and anti-CD8-FITC (Miltenyi Biotec). Fixation and permeabilization were performed using the BD Cyofix kit and BD Perm/Wash kit (Becton Dickinson) ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was conducted using anti-TNFα-APC and anti-IFN-γ-PE antibodies (Miltenyi Biotec). A VioBlue-live-dead marker (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... HLA-typing was performed via HLA-surface staining with anti-HLA*02-APC-antibody (Miltenyi Biotec) and anti-HLA-A*03-FITC-antibody (Milteny Biotec) ...
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bioRxiv - Immunology 2024Quote: ... and Flt3-Ligand (Bio-Techne) A fresh aliquot of donor cells was thawed and the MACS CD8+ T-cell isolation kit (Miltenyi Biotec) was used according to the manufacturers instruction to enrich for CD8 positive cells ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of 0.2% Tween-20/1X PBS containing 1 μL of PE-Foxp3 (Miltenyi Biotec cat# 130-111-678) was added and cells were incubated for 30 min in the dark ...
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bioRxiv - Microbiology 2024Quote: ... neutrophils were isolated via the use of a MACSxpress negative antibody collection kit (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... spleen cells were depleted of B220- and CD62L-expressing cells via magnetic bead-based depletion (Miltenyi Biotec). Cells from anti-ARTC2 NB-treated or untreated donor mice were labelled with CTV (Invitrogen ...
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Kir6.1, a component of an ATP-sensitive potassium channel, regulates natural killer cell developmentbioRxiv - Immunology 2024Quote: ... spleens were dissociated with the gentleMACS spleen disassociation kit (Miltenyi Biotec). Cells were then filtered ...
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bioRxiv - Microbiology 2024Quote: ... Neural tissue dissociation kit (Miltenyi Biotec, 130-093-231) was used according to the manufacturer’s protocol ...
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Kir6.1, a component of an ATP-sensitive potassium channel, regulates natural killer cell developmentbioRxiv - Immunology 2024Quote: ... and NK cells were isolated using a negative selection method (NK Cell Isolation Kit, Miltenyi Biotec) following the manufacture’s guidelines ...
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Kir6.1, a component of an ATP-sensitive potassium channel, regulates natural killer cell developmentbioRxiv - Immunology 2024Quote: ... NK-specific Kcnj8 KO and WT mice were injected intraperitoneally with 100 µg poly I:C (Miltenyi Biotec) in 200 µL sterile PBS ...
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bioRxiv - Microbiology 2024Quote: ... and separated by MACS (Miltenyi Biotec). Newly infected RPE-1 or HUVEC cells were maintained for a further 48 h prior to CD107a assay.
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bioRxiv - Immunology 2024Quote: ... were co-cultured with Siglec-Fhi or Siglec-Flow neutrophils (0.05 million) in the presence of 5 ng/ml TGFβ (Miltenyi Biotec), 20 ng/ml IL-6 (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... Dead cells were removed using a dead cell removal kit (Miltenyi Biotec). The single-cell suspensions were then stained with TotalSeq™-C Mouse antibody ...
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bioRxiv - Immunology 2024Quote: ... and enriched by negative selection mouse B cell isolation kit (Miltenyi Biotec, #130-090-862 ...
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bioRxiv - Immunology 2024Quote: ... the other fraction was depleted of lineage-positive (Lin+) cells using a Direct Lineage Cell Depletion Kit (Miltenyi Biotec, Cambridge, MA, USA, 130-110-470) containing antibodies to CD5 ...
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bioRxiv - Immunology 2024Quote: ... Human liver cell suspensions were generated either by gentle grinding tissue pieces through a 70µM nylon cell strainer or via mechanical separation using a gentleMACS Dissociator (Miltenyi Biotec). Liver cell suspensions were subjected to density gradient centrifugation using 33% Percoll prior to treatment with red blood lysis buffer ...
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bioRxiv - Immunology 2024Quote: ... CD8 T cells from autologous blood and tonsils from 4 HLA-A*02:01 donors were enriched using negative selection magnetic beads (CD4, CD19 depletion, Miltenyi Biotec). Roughly 1.5 e6 enriched CD8s were stained with Fc block for 10 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from spleen homogenates of mice using CD4 MicroBeads (Miltenyi Biotec, 130-117-043), followed by CFSE-VPD450 labeling ...
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bioRxiv - Immunology 2024Quote: ... sorted with a MACS mouse untouched CD8+ T cells Isolation Kit (Miltenyi Biotec) from splenocytes ...
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bioRxiv - Immunology 2024Quote: ... Neutrophils were isolated using the MACSxpress Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions within 1–2 hours of blood draw ...
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bioRxiv - Immunology 2024Quote: ... erythrocyte depletion (Miltenyi Biotec) was performed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells (tNGFR+) were sorted using MACSelect LNGFR microbeads (Miltenyi Biotec, 130-091-330).
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bioRxiv - Immunology 2024Quote: ... Monocytes were isolated using CD14 microbeads (Miltenyi Biotec, 130-050-201) and plated at 150’000 cells/cm2 in F+ high attachment plates (Sarstedt ...
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bioRxiv - Immunology 2024Quote: ... magnetic-activated cell sorting (MACS) was performed using Anti-Ly6G Microbeads Ultrapure (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured at 37°C ...
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bioRxiv - Immunology 2024Quote: ... The lymphoid organs were then ground through a 30 μm filter mesh (Miltenyi Biotec, Bergisch Gladbach, Germany) and washed with HBSS/BSA (Sigma Life Science ...