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Citations for Miltenyi Biotec :
1151 - 1200 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Debris (primarily myelin debris) was then removed using Debris Removal Solution (Miltenyi Biotec, 130-109-398) and the resulting cells were magnetically sorted for GLAST+ astrocytes according to the manufacturer’s protocol (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... and the resulting cells were magnetically sorted for GLAST+ astrocytes according to the manufacturer’s protocol (Miltenyi Biotec, 130-096-052).
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bioRxiv - Neuroscience 2024Quote: ... 2 μM Purmorphamine (Miltenyi Biotec), 100 ng/mL FGF8 (Peprotech) ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec) were also added to the medium ...
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bioRxiv - Molecular Biology 2024Quote: ... Add 600µL of debris removal solution (Miltenyi Biotec, Cat# 30-109-398), then 2mL of the resuspension buffer (0.5% BSA ...
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bioRxiv - Microbiology 2024Quote: ... parasitised erythrocytes (on some occasions separated from uninfected erythrocytes using a Miltenyi Biotec VarioMACS magnet) were fixed with 4% paraformaldehyde and 0.0075% glutaraldehyde in 1×PBS for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... human from Miltenyi Biotec (130-094-532) was used for mitochondria fractionation following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... stained with anti-His antibody (Antibody and Reagents Table) followed by anti-mouse-IgG magnetic beads (Miltenyi Biotec), and separated by MACS (Miltenyi Biotec) ...
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bioRxiv - Plant Biology 2024Quote: ... Immunoprecipitation was carried out using micromagnetic bead-coupled GFP antibodies from µMACS GFP Isolation Kit (Miltenyi Biotec 130-091-125) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Treg cells were then isolated from PBMCs using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Murine T cells were isolated from C57Bl6/J (WT or IFNγ-/-) splenocytes with CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Primary human monocytes were positively enriched from buffy coat PBMCs with CD14 Microbeads and LS columns (Miltenyi Biotec). TrueCut Cas9 Protein v2 and TrueGuide Synthetic single guide (sg)RNAs (both from Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... followed by Miltenyi Biotec magnetic-assisted CD14+ cell sorting ...
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bioRxiv - Immunology 2024Quote: ... TOX (PE, REA473, Miltenyi Biotec); H3K27 (PE-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were purified from PBMCs by magnetic cell sorting using CD14 Microbeads (Miltenyi Biotec). Monocytes (> 95% CD14+ cells ...
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bioRxiv - Immunology 2024Quote: ... BRD1-KO cells were re-stimulated with anti-CD3 and anti-CD28 T cell activation beads (TAB, Miltenyi Biotec) and cytokines (IL-1β and IL-23 ...
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bioRxiv - Immunology 2024Quote: ... Memory CD4+ T cells were isolated using an untouched negative isolation kit (Miltenyi Biotec). Isolated memory CD4+ T cells were stimulated with plate bound α-CD3 (5 µg/ml ...
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bioRxiv - Immunology 2024Quote: Lung and spleen dissociation kits (Miltenyi Biotec) were used to dissociate lung and mLN into single cell suspensions ...
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bioRxiv - Genomics 2024Quote: ... Samples were then loaded onto µMACS columns placed in the magnetic field of a µMACS Separator (Miltenyi Biotec), washed 4 times with cold IP buffer ...
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bioRxiv - Genomics 2024Quote: ... while the rest was incubated with 40µl µMACS anti-c-myc MicroBeads (Miltenyi Biotec) for 1h at 4°C with constant rotation ...
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bioRxiv - Microbiology 2024Quote: ... Tissue samples were collected in MACS tissue storage solution (Miltenyi Biotec, 130-100-008) at necropsy and processed immediately for single cell isolation as described previously.31,37 Isolated cells were stained for surface markers and analyzed by flow cytometry or vector copy number was determined by digital PCR.
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bioRxiv - Microbiology 2024Quote: ... CD34+ cells were isolated from fetal livers using anti-CD34+ magnetic bead-conjugated monoclonal antibodies (Miltenyi Biotec) and transduced with vectors described above ...
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bioRxiv - Microbiology 2024Quote: ... and with recombinant human M-CSF (rh-MCSF Miltenyi Biotec) at a concentration of 25 ng/ml for 6 days at 37°C with 5% CO2 in a humidified incubator ...
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bioRxiv - Immunology 2024Quote: ... were isolated from spleens and lymph nodes of donor mice using magnetic cell separation (MACS) using mouse CD8a+ T cell isolation kits (Miltenyi Biotec), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Lymphocytes were isolated from lymph nodes and spleens and made into single-cell suspensions using a syringe plunger and 100µm or 70µm cell strainers (MACS SmartStrainer, Miltenyi Biotec). ∼3×106 cells were used in subsequent staining steps for flow cytometry analysis ...
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bioRxiv - Immunology 2024Quote: ... cells were enriched by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-075), and 1 × 103 OT-I T cells in 100 mL of dPBS were transferred by retroorbital injection into recipient mice.
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bioRxiv - Immunology 2024Quote: ... Lungs were perfused with PBS prior to collection and mechanically dissociated with the GentleMACS dissociator (Miltenyi Biotec, Sydney, Australia) before enzymatic digestion with DNAse I (10 U/mL ...
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bioRxiv - Microbiology 2024Quote: ... Digestion was performed using the GentleMACS Dissociator with the Lamina Propria Dissociation Kit (Miltenyi Biotec). Immune cells were isolated using a Percoll gradient ...
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bioRxiv - Immunology 2024Quote: ... Human primary NK cells were isolated from the cryopreserved PBMCs utilizing the NK Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-092-657) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... was placed tightly in the QuadroMACS™ Separator (Miltenyi Biotec). Total alive CD4+ T cell number was determined by TC20 automated cell counter using Trypan Blue dye.
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bioRxiv - Immunology 2024Quote: ... one LC column (Miltenyi Biotec) was placed tightly in the QuadroMACS™ Separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... a microbead kit (CD115 Microbead Kit; Miltenyi Biotec, Bergisch Gladbach, Germany) was used to isolate these cells from the whole blood of C57BL/6 and CCR2-/- mice ...
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bioRxiv - Immunology 2024Quote: ... Monocytes from bone marrow were enriched by negative selection (Monocyte Isolation Kit BM, Miltenyi Biotec) following the manufacturer’s instructions and resuspended in sterile PBS (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... Human monocytes were purified from healthy donors’ PBMCs using anti-human CD14 magnetic microbeads and MACS cell separation columns (Miltenyi Biotec; Germany), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... with a an IL-10 secretion assay (Miltenyi Biotec; Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10 ng/mL recombinant human IL-2 (rhIL-2, Miltenyi Biotec, Germany) with a an IL-10 secretion assay (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... Infected cells suspensions were lysed using the gentleMACSTM dissociator with gentleMACSTM M tubes (Miltenyi Biotec, Bergisch Gladbach, Germany) and the “Protein” protocol ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 200U/mL recombinant human IL-2 (rhIL-2, Miltenyi Biotec, Germany). Fresh complete RPMI medium supplemented with rhIL-2 was replaced every 6 days and T-cell lines were used for the experiments (i.e. ...
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bioRxiv - Immunology 2024Quote: ... anti-IL-10 (Miltenyi Biotec, Germany), 1μg/ml anti-IL-10R (R&D ...
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bioRxiv - Immunology 2024Quote: ... PepTivator® EBV Consensus (Miltenyi Biotec, Germany), respectively ...
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bioRxiv - Immunology 2024Quote: ... MACS® Cytokine Secretion Assays for IFN-γ or IL-22 and IL-17A (Miltenyi Biotec, Germany) were performed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... the absence of exogenous Th17- promoting cytokines (ii) with 10ng/ml of human recombinant polarizing cytokines (i.e.,TGF-β, IL-4, IL-12; Miltenyi biotec, Germany) or WNT10a ...
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bioRxiv - Immunology 2024Quote: ... cells were blocked with 0.5 μg/mL anti-CD40 mAb (Miltenyi Biotec) and stained with anti- CXCR5 and CCR7 for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... the mice were sacrificed and their lungs were harvested and homogenized in lysis solution (ZR Fungal/Bacterial DNA MiniPrepTM, Epigenetics) using a gentleMACS dissociator (Miltenyi Biotec). The homogenates were clarified by centrifugation and the cytokine content of the supernatant was determined using a Luminex Multiplex Assay ...
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bioRxiv - Immunology 2024Quote: ... up to 107 cells were incubated with 5µl anti-CD31 microbeads and 10µl of anti-CD45 microbeads (Miltenyi Biotec, Germany), followed by LS column magnetic isolation ...
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bioRxiv - Immunology 2024Quote: ... Flowthrough cells were incubated with 15µl of anti-EpCAM microbeads (Miltenyi Biotec, Germany) followed by magnetic isolation with MS columns ...
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bioRxiv - Immunology 2024Quote: ... donor OT1 cells that were present at low numbers post adoptive transfer were magnetically enriched with magnetic columns (LS Columns, Miltenyi Biotec). For early (≤ 72 h after immunization or infection ...
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bioRxiv - Immunology 2024Quote: ... on an autoMACS Pro Separator (Miltenyi Biotec). Naïve T cells (106 cells/ml ...
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bioRxiv - Immunology 2024Quote: ... mouse (Miltenyi Biotec). Total RNA was isolated from the DCs using TRIzol (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... and put into gentleMACS C-tubes (Miltenyi Biotec, #130-093-237) with dissociation enzymes prepared for initial tissue homogenization using the 37C_ABDK_01 protocol on the gentleMACS Octo-Dissociator with heaters (Miltenyi Biotec ...