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Citations for Miltenyi Biotec :
1051 - 1100 of 1616 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Dead cells were removed by using a dead cell removal kit and MS columns (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Single-cell RNA-seq libraries were made with the 10x Genomics Chromium Next GEM Single Cell 3’ Library Kit (v3.1 ...
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bioRxiv - Cell Biology 2020Quote: ... Lineage positive cells were depleted using a mouse lineage cell depletion kit (Miltenyi Biotec, 130-090-858, 130-110-470) and the following antibodies from BioLegend CD150 (Cat#115913 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed with magnetic cell-sorting (MACS) buffer (PBS supplemented with 0.5% BSA and 5 mM EDTA) and incubated with CD117 (KIT) MicroBeads (Miltenyi Biotec) on ice for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... cell selection for C-kit positive cells was performed with a CD117 antibody (Cat # 120-099-672, Miltenyi Biotec, Germany) in a Miltenyi Mini Macs System ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from spleen of P2Y6fl/fl and P2Y6ΔNcr1 mice fed a normal chow diet using the mouse NK cell isolation kit from Miltenyi Biotec, (Bergisch Gladbach ...
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bioRxiv - Immunology 2021Quote: ... Human naïve B cells were isolated using the MACSxpress Whole Blood B Cell Isolation Kit (Miltenyi Biotec, 130-098-190) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... or for scRNAseq following an additional step of dead cell removal using the MACS Dead Cell Removal Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPLEX Cytokine 10 kit (Miltenyi Biotec). Also ...
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bioRxiv - Immunology 2020Quote: ... Primary murine monocytes were isolated from C57BL/6J bone marrow using the Monocyte Isolation Kit (Miltenyi Biotec, 130-100-629) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPlex Cytokine 10 kit (Miltenyi Biotec). Also ...
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bioRxiv - Immunology 2020Quote: ... CD34+ HSCs were isolated from the cord blood and perfused placental tissue by using the CD34 Microbead Kit (Miltenyi Biotec) and stored at −150°C ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were enriched magnetically by their expression of H2Kk using a MACSelect Transfected Cell Selection kit (Miltenyi Biotec, Sunnyvale, CA). Cas9-mediated indel formation at the Acvr1 or Bmpr1a locus was confirmed by T7E1 mismatch analysis on the enriched cell populations (T7E1 Nuclease – New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used immediately for scRNA-seq ...
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bioRxiv - Immunology 2022Quote: ... Splenic naïve CD4 T cells were purified using negative Naïve CD4+ T cell isolation Kit yielding a cell purity of 97%-99% (Miltenyi Biotec). For plasma cell differentiation ...
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bioRxiv - Immunology 2022Quote: Splenic and mLN naïve B cells were purified using negative B cell isolation kits yielding enriched populations of 95%–98% (Miltenyi Biotec). Splenic naïve CD4 T cells were purified using negative Naïve CD4+ T cell isolation Kit yielding a cell purity of 97%-99% (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... pan splenic T cells were isolated from crushed spleens of naïve C57BL/6 mouse using the Mouse Pan T Cell Isolation Kit (Miltenyi Biotec). In the subsequent co-culture incubation ...
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bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Genomics 2022Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used for scRNA-seq ...
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bioRxiv - Immunology 2022Quote: ... CD8+ SP thymocytes were isolated using the CD8a+ (Ly-2) positive selection kit per manufacturer’s instructions (Microbeads, #130-117-044; Miltenyi Biotec). Flow-through resulted in the DN population ...
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bioRxiv - Neuroscience 2022Quote: ... followed by dissociation and isolation using the CD11b isolation kit according to the manufacturer’s protocol (130-092-628 and 130-126-725, Miltenyi Biotec). Primary microglia purity was examined by flow cytometry after staining by Cd11b-FITC (130-113-243 ...
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bioRxiv - Immunology 2022Quote: Treg and non-Treg cells were enriched using MACS CD4+CD25+CD127dim/− Regulatory T cell isolation Kit human (Miltenyi Biotec) from PBMCs of healthy donors and plated (1 × 105 cells per well ...
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bioRxiv - Immunology 2022Quote: ... Macrophages were enriched using a magnetic-based commercial F4/80 macrophage positive selection kit (Miltenyi Biotec, MD, USA; Supplemental 3B) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Microbiology 2023Quote: ... NK cells were then re-purified by negative selection using purification kit and magnetic columns according to manufacturer’s instructions to reach purities of approximately 98% (Miltenyi Biotec, MS columns and isolation kit ref no.130-115-818) ...
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bioRxiv - Immunology 2022Quote: ... naïve CD8 T cells were isolated from the spleen of pmel mice (having CD8 T cells bearing T cell receptors specific for the gp100 antigen) by magnet-assisted cell sorting (Naïve CD8+ T cell isolation kit, Miltenyi Biotec) and stained with Cell Trace Violet (CTV ...
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bioRxiv - Neuroscience 2022Quote: ... spinal cord tissues were homogenized using the Adult Brain Dissociation Kit (mouse and rat, cat # 130-107-677, Miltenyi Biotec) and running the gentleMACS program 37C_ABDK_02 following the manufacturer instructions.
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bioRxiv - Plant Biology 2022Quote: ... The corresponding supernatant was used for immunoprecipitation assay with anti-FLAG micro beads according to the manufactureŕs instructions (μMACS Epitope Tag Protein Isolation Kit (MACS Miltenyi Biotec)) ...
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bioRxiv - Molecular Biology 2023Quote: ... Hearts were mashed with pestles for 2 min and cell isolation performed according to manufacturer’s instructions (Neonatal Heart Dissociation Kit, Miltenyi Biotec). Cell suspension was centrifuged for 5 min at 600 x g and cells were resuspended in cell culture medium and plated in 22.1 mm plates ...
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bioRxiv - Microbiology 2023Quote: ... and single-cell suspension was prepared using the mouse lung dissociation kit (Cat: 130-095-927; Miltenyi Biotec, Gaithersburg, MD) and GentleMACS™ Dissociator treatment following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The cortices were dissociated into a single cell suspension using a neural tissue dissociation kit (Miltenyi Biotec; 130-092-628). Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were purified from spleen and lymph nodes of 6-15 week old 2D2 mice or OT-II mice using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD4 naïve T cells were isolated from pooled lymph nodes and spleens using the naïve CD4 T cell isolation kit (130-104-453, Miltenyi Biotec) and stained with BioTracker 655 Red Cytoplasmic Membrane Dye (SCT108 ...
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bioRxiv - Neuroscience 2023Quote: ... frozen hippocampus of 3 mice in each sample group were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec, Germany), and after dead cell removal ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells were then obtained using a negative magnetic bead-based selection kit for pan T-cell isolation following the manufacturer’s protocol (Miltenyi Biotec). A negative selection was chosen to minimize any activation of T-cells during the selection process ...
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bioRxiv - Microbiology 2023Quote: ... Cells from the colonic lamina propria (cLP) were isolated using a Lamina Propria Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) on the gentleMACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The flow-through was then subjected to MuSC enrichment using the satellite cell isolation kit (Miltenyi Biotec 130-104-268). Once each population was isolated ...
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bioRxiv - Immunology 2023Quote: ... enzymatic digestion and tissue dissociation of the left lateral liver lobe was performed using a GentleMACS Octo Dissociator with heaters and the liver perfusion kit for mice according to the manufacturer’s protocol (Miltenyi Biotec). Hepatocytes were separated from lymphocytes by centrifugation at 50× g for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec) into complete RPMI media ...
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bioRxiv - Immunology 2023Quote: ... or CD45.2+ 4get-GFP OT-II T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec). The average purity of OT-II cells was approximately 75-80% for all experiments ...
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bioRxiv - Immunology 2023Quote: ... 8.3 CD8 T cells were isolated from the LNs of non-diabetic NY 8.3 NOD female mice using two rounds of the CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were strained (40 μm filters) and dead cells removed using a dead cell removal kit according to the manufacturer’s instructions (Miltenyi Biotec). Cell viability was determined using trypan blue staining and cells were counted using a haemocytometer ...
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bioRxiv - Immunology 2023Quote: ... or Panx1-KO (CD4-Cre Panx1fl/fl) CD8+ T cells were isolated from naïve mice with the Mouse CD8+ T cell isolation kit (Miltenyi Biotec). Cells were stimulated for 24h ...
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bioRxiv - Immunology 2023Quote: OT-I cells were isolated from spleen of CD90.1 OT-I mice using Naive CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturers’ guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... Pan B cells were isolated from peripheral blood leukocytes using the Pan B cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2023Quote: ... Astrocytes were isolated from the remaining cell suspension using the anti-ACSA-2 microbead kit (130-097-678, Miltenyi Biotec). The cell suspension was incubated with FcR blocking reagents followed by anti-ACSA-2 microbeads and placed on a magnetic MACS separator for positive selection ...
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bioRxiv - Cancer Biology 2023Quote: Naïve CD4+ T cells and nTregs were isolated from the spleens of healthy C57B6129SF1/J mice by magnetic separation using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec). The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution ...