Labshake search
Citations for Miltenyi Biotec :
1301 - 1350 of 1616 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Both CD45 nondepleted and CD45-depleted single-cell suspensions were depleted of dead cells using a Dead Cell Removal kit (catalog no. 130-090-101; Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Part of the single-cell suspension was depleted of CD45+ cells to enrich for tumour cells using a CD45 MicroBeads kit (catalog no. 130-045-801; Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cell lysis was performed with ACK buffer (Thermo) before mouse cells were removed from the dissociated lungs using the mouse cell depletion kit (Miltenyi Biotec) yielding purified MeWo cells that achieved successful lung colonization ...
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bioRxiv - Bioengineering 2023Quote: ... Lung progenitor cultures at day 14 of differentiation were detached as described above and fixed and permeabilized using the transcription factor staining buffer kit (Miltenyi Biotec). After washing with flow buffer (PBS w/o containing 1.0% BSA) ...
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bioRxiv - Cancer Biology 2023Quote: ... remaining cells were processed for T cell purification using the Pan T cell isolation kit (Miltenyi Biotec, cat # 130-095-130). Cell viability of the purified T cells was ascertained by labeling with the eBioscience™ Fixable Viability Dye eFluor™ 506 for 30 min on ice ...
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bioRxiv - Genetics 2023Quote: PBS-perfused brains were cut into small pieces and dissociated using the Adult Brain Dissociation Kit and gentleMACS Octo Dissociator with heaters (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Lineage-positive cells were depleted from the resulting cell suspensions using the mouse direct lineage cell depletion kit (cat. 130-110-470, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used to lyse erythrocytes from bone marrow cell suspension followed by T cells depletion using mouse CD3ε MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ T cells were magnetically isolated from the spleens of healthy C57B6129SF1/J mice using a mouse CD4+ T cell isolation kit (Miltenyi Biotec). The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution ...
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bioRxiv - Cancer Biology 2023Quote: Primary BM mononuclear cells isolated from MM patients or healthy donors were enriched in CD138+ PCs using magnetic sorting with the CD138 Microbead Kit (Miltenyi Biotec). Cells were plated in 48-well plates previously seeded with human BM-derived mesenchymal cells.
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bioRxiv - Synthetic Biology 2023Quote: ... were isolated using Ficoll gradient centrifugation and human T cells were obtained by magnetic sorting using the eHuman Pan T cell isolation kit (Miltenyi Biotec). Once isolated ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells from the sample were purified by magnetic bead separation using the CD4+CD62L+ isolation kit (Miltenyi Biotec). Cells were plated in 96-well plates coated with anti-CD3 antibody (5μg/ml ...
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bioRxiv - Immunology 2023Quote: Autologous CD8 T cells were obtained from frozen PBMCs from the same donor using the CD8+ Microbeads human T cell isolation Kit (Miltenyi Biotec). To monitor cell proliferation ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC were differentiated into cells of all three germ layers using the StemMACS Trilineage Differentiation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Briefly ...
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bioRxiv - Genetics 2023Quote: ... gentle and rapid generation of single-cell suspensions was achieved by using a modified version of the procedure of a mouse Lung Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Systems Biology 2024Quote: ... we first processed a portion of the cell harvest solution using the dead cell removal kit (Miltenyi Biotec 130-090-101) to remove any cell debris to avoid any free RNA from lysed cells from getting mixed in with mRNA to be extracted from live cells ...
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bioRxiv - Synthetic Biology 2024Quote: The mixed mouse primary neonatal cardiomyocyte cell culture was obtained using a neonatal heart dissociation kit (Miltenyi Biotec, 130-098-373) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Brain tissue was dissociated into a single-cell suspension by enzymatic digestion using the Adult Brain Dissociation Kit P (# 130-107-677, Miltenyi Biotec) and the gentleMACS™ Octo Dissociator (# 614 130-096-427 ...
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bioRxiv - Neuroscience 2024Quote: ... according to the manufacturer’s instructions including a red blood cell removal and a debris removal step (Adult Brain Dissociation Kit P, # 130-107-677, Miltenyi Biotec). Isolated cells were washed twice with D-PBS (# 14040-133 ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was immediately dissociated to single-cell suspension by enzymatic degradation and myelin removal using the adult brain dissociation kit and GentleMACS dissociator (Miltenyi Biotec.). The isolated tissues were placed in C-tubes ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were obtained from the lymph nodes (LNs) and spleens of OT-1 mice using a negative selection kit (130-104-075, Miltenyi Biotec). For CTL differentiation ...
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bioRxiv - Immunology 2023Quote: ... naive CD4+ T cells (CD4+CD25−CD62L+) were sorted from the spleen of WT C57Bl/6 mice using a commercially available kit (Miltenyi Biotec). Next ...
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bioRxiv - Neuroscience 2023Quote: ... a single cell suspension from the hippocampus and cortex was prepared using Neural Tissue Dissociation Kit (papain-based) (Miltenyi Biotec GmbH). After blocking with CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The single cell suspension was then depleted of dead cells and debris using magnetic-activated cell sorting (MACS, Dead Cell Depletion Kit, Miltenyi Biotec). Cells were then distributed drop-wise onto a Seq-Well microwell array preloaded with mRNA capture beads and processed as described previously5 ...
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bioRxiv - Cancer Biology 2023Quote: Primary tumour epithelial cells were obtained using the gentle MACS Dissociator and Mouse Tumor dissociation kit (Miltenyi Biotec, Bisley, Surrey, UK) following the protocol recommended for ‘Dissociation of Tough Tumors’ ...
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bioRxiv - Immunology 2023Quote: ... IEC/IEL single cell suspensions were prepared as above and dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec) using LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... OT-I T cells were then purified by negative magnetic cell sorting using mouse CD8α+ T-cell isolation kit (130-104-075; Miltenyi Biotec) according to the manufacture’s protocols ...
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bioRxiv - Immunology 2023Quote: ... spleens and tumors from groups of mice were harvested and tumors were processed using the Mouse Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Sarcoma tissue was collected on ice and immediately cut into 2 mm3 segments and dissociated to a single-cell suspension using the Human Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... spleens and tumors from groups of mice were harvested and tumors were processed using the Mouse Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 1×106 vehicle or ACCi-conditioned OT-1 T cells were infused into B16-OVA tumor bearing mice 10 days after tumor challenge and tumors were harvested 7 days later using the Mouse Tumor Dissociation Kit and gentleMACS Octo dissociator with heaters (Miltenyi Biotec) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2023Quote: ... cerebellum of WT and KIKO mice was dissociated to single-cell suspension using adult brain dissociation kit from MACS Technology and using GentleMACS (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Skeletal muscle myoblasts derived from WT and KO ADAMTSL1 mice were isolated using the Satellite Cell Isolation Kit MACS protocol (Miltenyi Biotec), as described previously34.
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bioRxiv - Immunology 2023Quote: ... B cells were first enriched from the splenocyte suspension by MACS (magnetic activated cell sorting) using the Pan B cell Isolation Kit (Miltenyi Biotec), followed by staining for FACS (fluorescence activated cell sorting) ...
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bioRxiv - Cell Biology 2023Quote: ... One ovary from each mouse was dissociated using a Multi Tissue Dissociation Kit 1 (cat# 130110201, Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions to create a single-cell suspension in D-PBS (cat# 14287080 ...
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bioRxiv - Physiology 2024Quote: ... Brains without the cerebellum and olfactory bulbs were collected and dissociated by using an Adult Brain Dissociation Kit and a gentleMACS Octo Dissociator with Heater (Miltenyi Biotec). Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage ...
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bioRxiv - Cancer Biology 2023Quote: The omental tumors harvested from C57Bl/6 mice were minced and dissociated using the Human Tumor Dissociation Kit from Miltenyi Biotec, following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue was sectioned into pieces and a portion of tumour or normal brain was dissociated using the MACS Brain Tumor Dissociation Kit (Miltenyi Biotec). Labelled engrafted GFP+ cells were isolated from dissociated cell population using a BD FACSAria Fusion flow cytometer machine at the McGill University Health Centre Research Institute platform ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated from PBMCs through two rounds of magnetic enrichment using the human pan B cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: CD4 T cells were isolated from the spleens of donor mice and purified using the mouse CD4 T Cell Isolation Kit (Miltenyi Biotec). The purity and cell count of donor CD4 T cells were assessed using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated from healthy donors via magnetic bead labeling (CD8+ T cell isolation kit, Miltenyi Biotec, Germany). U937 (ATCC ...
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A framework for defining mesenchymal cell types associated with murine periosteal and endosteal bonebioRxiv - Genomics 2023Quote: ... cells were depleted of hematopoietic cells and endothelial cells using a lineage depletion kit (Miltenyi Biotec, cat. no. 130-090-858) followed by CD45 ...
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bioRxiv - Microbiology 2024Quote: ... Norway) centrifugation and used for CD4+ T cell isolation by negative selection with a commercial kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the freshly collected whole lungs were dissected into single lobes to isolate single cells using a mouse lung dissociation kit (Miltenyi Biotec) and a gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2024Quote: We isolated single cells from the dissected mPFC sections using a neural tissue dissociation kit (Cat# 130-092-628, Miltenyi Biotec). After cell concentration quantified ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... human (130-050-501) combinations based on the Mini & MidiMACS™ Starting Kit (130-091-632) (all from Miltenyi Biotec, Germany) in accordance with the manufacturer’s protocols.
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bioRxiv - Developmental Biology 2024Quote: ... containing enzymatic digestion solution according to manufacturer’s protocol for the Neonatal Heart Dissociation Kit (Miltenyi Biotec, Cat-Nr: 130-098-373). Two mouse hearts were processed in separate C-tubes at the same time for all experiments presented in this manuscript ...
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bioRxiv - Immunology 2024Quote: ... Two hundred thousand T cells per well were cultured in 96-well plates in the presence of T Cell Activation/Expansion Kit (Miltenyi Biotec) for 2 weeks ...
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bioRxiv - Immunology 2024Quote: ... through depletion of non-target cells by anti CD14/ CD15/CD16/CD19/CD34/CD36/CD56/CD123/CD235a biotin-conjugated monoclonal antibodies MicroBeads Kit (Miltenyi Biotec), with the use of a LS column ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was dissociated for 15 min at 37 °C on the GentleMACS Octo Dissociator with Heaters (Miltenyi) using Human Tumor Dissociation Kit (Miltenyi Biotec). Following tissue dissociation ...