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Citations for Miltenyi Biotec :
1001 - 1050 of 1616 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... were washed in PBS and MACS buffer (PBS, 2 µM EDTA, 0.5% BSA) and stained with Human CD34 Microbead kit (Miltenyi Biotec) following the manufacturer’s instruction for 30 minutes (4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Lineage negative cells were collected by applying the Direct Lineage Cell Depletion Kit mouse (130-090-858, Miltenyi Biotec GmbH) and magnetic separation following manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... CD34 positive cells were then bead enriched using the CD34 MicroBead Kit UltraPure (Miltenyi Biotec, catalog number 130-100-453) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the biopsies were cut into 2-3mm pieces and dissociated using a human Tumour Dissociation Kit (Miltenyi Biotec, Surrey, UK) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Immunology 2021Quote: ... Allogenic naïve CD4+ T cells and CD8+ T cells were isolated from buffycoats using the naïve CD4+ T cell Isolation kit II (Miltenyi Biotec) and CD8+ T cell Isolation kit (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2020Quote: ... Single cell suspensions were generated using the Neural Tissue Dissociation Kit – Postnatal Neurons (Miltenyi Biotec. Cat no. 130-094-802), following manufacturer’s instructions for manual dissociation ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspension was obtained by automated dissociation using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Cat#130-092-628) and the gentleMACS™ Dissociator (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2020Quote: ... Cardiac cells were isolated by excluding dead cells using the MACS dead cell removal kit (Miltenyi Biotec, 130-090-101) and were suspended in the optimized culture medium for cardiac cell culture ...
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bioRxiv - Neuroscience 2020Quote: EV preparation by immunobead isolation was performed using the Pan (mixture of CD9-, CD63- and CD81)-Exosome Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec). 2 ml of Amicon Ultra-15 (100 kDa cutoff ...
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bioRxiv - Cancer Biology 2020Quote: ... polyA+ RNA was purified using the 96-well MultiMACS mRNA isolation kit on the MultiMACS 96 separator (Miltenyi Biotec, Germany) from total RNA with on column DNaseI-treatment as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the brain tissues (cortices and hippocampi) were enzymatically dissociated with Neural Tissue Dissociation Kit-P (#130-092-628; Miltenyi Biotec). Myelin was removed by MACS Myelin Removal Beads II (#130-096-733 ...
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bioRxiv - Microbiology 2020Quote: ... T cells from the lungs of naïve or infected mice (day 8 pi) were enriched using the Pan T cell Isolation Kit II (Miltenyi Biotec) and co-cultured with infected BMDC at a ratio of 10:1 for 2-3 hours followed by the addition of Brefeldin A (5µg/ml ...
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bioRxiv - Microbiology 2020Quote: ... In vivo infections were performed by intranasal injection of 5×10^5 bacteria per mouse Lungs were collected after 20h from infection and digested using the Lung Dissociaton Kit according to the manufacturer’s instructions (Miltenyi Biotec) plus added Dispase II at 0,1U/ml (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... tumors (0.5 g) were mechanically disrupted by chopping and then chemically digested using Tumor Dissociation Kit (130-096-730, Miltenyi Biotec) and gentleMACS Dissociator (130-096-427 ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-EXO70B2 and free-GFP as a control were isolated using the μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells were purified via negative selection using the Naïve CD4+ T cell Isolation mouse Kit (Miltenyi Biotec) according to the manufacturer’s recommendations.
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bioRxiv - Cancer Biology 2022Quote: ... Single mouse tumor cell suspensions were obtained using a mouse Tumor Disassociation Kit from Miltenyi Biotec (Cat# 130-096-730). After leukocytes extraction using density gradient medium ...
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bioRxiv - Molecular Biology 2022Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2022Quote: ... P14 and P21 mice cortices during an additional step using the debris removal kit (Miltenyi Biotec; ref 130-090-101). Cells were suspended in a 0.5% NGS solution then incubated with anti-PDGFRα or anti-O4 coupled-beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... total CD4+ T cells were directly isolated from buffy coats using the StraightFrom Buffy Coat CD4 MicroBead kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Primary NK cells were isolated and enriched the day of the experiment from the spleens of 8-12 week old female C57BL/6 mice using the NK Cell Isolation Kit II according to manufacturer’s instructions (Miltenyi Biotec). 50,000 NK cells were then seeded in the top chamber of a transwell insert (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... then astrocytes were isolated using the Miltenyi Biotec’s anti-ACSA-2 magnetic cell sorting kit and protocol (Miltenyi Biotec, 130097678). RNA was purified and concentrated with proteinase K cell digestion ...
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bioRxiv - Immunology 2022Quote: Neutrophils were purified from freshly isolated mouse bone marrow cell suspensions using an autoMACS pro-selector cell separator with a MACS neutrophil isolation kit for negative selection in accordance with the manufacturer’s instructions (Miltenyi Biotec). Neutrophils were aged overnight in serum-free medium at 3 × 106 cells/ml at 37 °C and 5 % CO2 ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2020Quote: ... For the isolation of pDCs from PBMCs the Plasmacytoid Dendritic Cell Isolation Kit II from Miltenyi Biotec (# 130-097-415) was used ...
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bioRxiv - Developmental Biology 2022Quote: ... spheres were dissociated using the MACS EB Dissociation Kit (order no. 130-096-348) according to manufacturer’s instruction (Miltenyi Biotec). The cell pellet was resuspended using Noci-3 medium (see Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were dissociated using TrypLE Express and enriched for CD34+ endothelial progenitor cells using the CD34 MicroBead Kit (Miltenyi Biotec). Purified cells resuspended in EGM-2 media (Lonza ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Immunology 2022Quote: RM CD4+ T cells were isolated from PBMCs using the non-human primate CD4+ T cells isolation kit (Miltenyi Biotec), activated using the NHP T-Cell Activation/Expansion kit (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2019Quote: ... Dead cells were removed using magnetic bead-bound antibodies against apoptotic and necrotic cells (Dead cell removal kit, Miltenyi Biotec) and cells were washed twice ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated untouched using the magnetic human Pan-T-Cell Isolation Kit according to the manufacturer’s protocol (MACS, Miltenyi Biotec).
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bioRxiv - Cancer Biology 2019Quote: ... Tumor tissues were mechanically and enzymatically dissociated using a human tumor dissociation kit (Miltenyi Biotec, Cat. No. 130-095-929), following the manufacturers recommendations ...
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bioRxiv - Immunology 2019Quote: ... Pan T cells were isolated from peripheral blood leukocytes using the Pan T cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec). Isolated human T cells were cultured in X-VIVO-15 medium supplemented with 2 % human serum.
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bioRxiv - Cell Biology 2020Quote: ... prior to separation into CD34+ and flowthrough (CD34−) cells using magnetic beads (CD34 Microbead Kit: Miltenyi Biotec, 130-046-702).
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bioRxiv - Systems Biology 2021Quote: ... these mice were euthanized and the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (130-104-075, Miltenyi Biotec), resuspended in a concentration of 106/ml ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T-cells were first isolated from HLA-DP4+ asymptomatic carriers using the Pan T-cell Isolation Kit (Miltenyi Biotec) and biotin-labeled anti-CD8 mAb (RPA-T8 ...
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bioRxiv - Genomics 2021Quote: ... BMMNC were either cryopreserved in FBS with 10% of DMSO or previously enriched for CD34+ (CD34 MicroBead Kit, Miltenyi Biotec) before cryopreservation.
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bioRxiv - Immunology 2021Quote: ... Naïve murine CD8+ T cells were isolated using negative selection (Naïve CD8 T Cell Isolation Kit, Cat no. 130-096-543, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the sample was subjected to a “clean-up” step using Dead Cell Removal kit (Miltenyi Biotec, Catalog #130-090-101). Cells were washed again and resuspended in 500 ul of 0.04% BSA/PBS and counted ...
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bioRxiv - Immunology 2020Quote: ... we modified the Pan T cell negative Isolation magnetic-activated cell sorting (MACSR) kit (Miltenyi Biotec, Cat #130-096-535) by adding anti-CD45RA biotin (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: CAR-and ΔLNGFR-positive T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Monocyte-derived dendritic cells (MDDCs) were generated from plastic adherent or directly isolated monocytes (Monocyte Isolation Kit II, Miltenyi Biotec) through culture for 6 days in Roswell Park Memorial Institute medium (RPMI-1640 ...
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bioRxiv - Immunology 2019Quote: ... and T cells were isolated using the Pan T Cell isolation kit II according to the manufacturer’s instructions (Miltenyi Biotec). Naïve antigen presenting cells were isolated from an unimmunized (naïve ...
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bioRxiv - Immunology 2019Quote: ... WT mouse by depleting T cells from the spleen using the CD3ε microbead kit according to the manufacturer’s instructions (Miltenyi Biotec). T cells were stained with CellTrace Violet as described in the above section and plated in 96-well flat-bottom plates at 1.5×105 T cells per well with/without 2×104 antigen presenting cells isolated from naïve WT spleen ...
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bioRxiv - Immunology 2021Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2021Quote: ... OT-1 cells were purified from OT-1 transgenic mice using negative selection with CD8a+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... naïve splenic CD43− B cells were magnetically isolated using the B cell isolation kit (Miltenyi Biotec, Inc.; 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: NK cell effectors were enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion Kit (Miltenyi Biotec). Target and effector cells were plated at E:T ratio of 2:1 in a 96 well round bottom plate ...