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Citations for Miltenyi Biotec :
901 - 950 of 2875 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: B cells were isolated from spleens of WT female mice and enriched with a B cell isolation kit (Miltenyi Biotec MACS). The purity of isolated B cells was 96±0.8% ...
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bioRxiv - Immunology 2020Quote: ... such T cells were selected using a human IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (Miltenyi Biotec, Auburn, CA) following the manufacturer’s instructions as previously described.38–42 Post-4 PBMC sample cryopreserved after monocyte depletion was thawed and cultured overnight in Yssel’s media (Gemini Bio Products ...
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bioRxiv - Immunology 2020Quote: ... CD137-expressing CD4+ memory T cells cells were positively selected by staining with biotin-conjugated CD137 antibody (clone REA765; Miltenyi Biotec) followed by anti-biotin MicroBeads and applied to a new MS column ...
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bioRxiv - Immunology 2021Quote: ... Conventional T cells were enriched from FVB/N mouse spleen single-cell suspensions using the mouse Pan T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and transplanted with syngeneic BALB/c bone marrow cells (5 × 106) after T-cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). For retransfer experiments ...
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bioRxiv - Immunology 2021Quote: ... B cell isolation from cryopreserved PBMCs was carried out using Memory B cells Isolation Kit (MACS/Miltenyi Biotec, Bergisch Gladbach, Germany). Next ...
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bioRxiv - Immunology 2020Quote: ... anti-PDL1-PE/Cy7 and anti-FasL-APC to analyze surface markers on B cells involved in T cell suppression and with CD4-APC-Vio770 to investigate T cell proliferation by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... splenic B cells were enriched by magnetic microparticle purification using mouse B Cell Isolation Kit (Miltenyi Biotec, Cat#130-090-862) and then further sorted by gating CD11c+ B cells (CD19+CD11c+CD21- ...
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bioRxiv - Immunology 2020Quote: ... PolyPeptide group) were co-cultured with 105 OTII CD4+ T cells (purified with a CD4+ T cell isolation kit, Miltenyi Biotec) in RPMI medium (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from 10 to 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec). Isolated cells were washed three times with PBS ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMC using magnet activated cell sorting (MACS) using the Pan T cell Isolation Kit (negative selection) according to the manufacturer’s instructions (Miltenyi Biotec, Germany). Following separation ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25- cells were then obtained by depletion of CD25+ T cells using CD25 positive selection (CD25 microbeads II, Human, Miltenyi Biotec) and collecting the unlabeled cells.
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions obtained from spleens were processed with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec; Cat#: 130-104-075) and transferred via the tail vein into recipient mice.
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bioRxiv - Cancer Biology 2022Quote: ... After the centrifugation they were resuspended in MACS buffer and the magnetic separation was performed using LS MACS column (maximum 108 labeled cells) for positive selection and LD MACS column (maximum 108 labeled cells) for negative selection (Miltenyi Biotec). The purity of positive subpopulation was >70% and <99% for the negative subpopulation.
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bioRxiv - Bioengineering 2022Quote: ... mouse bone marrow neutrophils were sorted from the bone marrow cell suspension using a mouse neutrophil isolation kit that enriches for CD11b+Ly6G+ cells (Miltenyi Biotec; negative selection using a cocktail that contains anti-CD5 ...
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bioRxiv - Immunology 2022Quote: ... OT-1 T cells were isolated from the spleens OT-1 mice using negative selection with CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from peripheral blood lymphocytes by negative selection using a CD4 T-cell isolation kit II and magnetic cell separation (Miltenyi Biotec). A total of 50,000 CD4+ T cells were cultured in IMDM (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells obtained using Ficoll density gradient centrifugation (Lymphoprep, ELITechGroup) followed by CD8+ T cell isolation (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec) on buffy coats from HLA-A2.1+ human donors after written informed consent in accordance with the Declaration of Helsinki and in agreement with institutional guidelines ...
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bioRxiv - Immunology 2022Quote: ... ratio were purified by magnetic-activated cell sorting (MACS®) according to the manufacturer’s instructions (NK cell Isolation kit; Miltenyi Biotec). Total RNA from the samples was isolated using TRIzol® reagent following the manufacturer’s isolation protocol (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: Previously cryopreserved PBMCs were thawed and CD8+ T cells were enriched by negative selection using a nonhuman primate CD8+ T cell isolation kit (Miltenyi Biotec). RNA was isolated from the enriched CD8+ T cells by TRIzol/phenol chloroform extraction with DNase treatment ...
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bioRxiv - Microbiology 2023Quote: ... were gained from EDTA blood by density separation (Lymphocyte Separation Medium (AnproTech)) and CD8+ T-cells were isolated with the human CD8+ T-cell isolation kit (Miltenyi Biotec) and a MidiMACS LS-column according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 0.1 mg ml−1 DNAase I in RPMI-1640 medium and enriched by depleting CD45+ cell and TER119+ cells using MACS microbeads (Miltenyi Biotec). Cells suspensions were incubated with anti-CD45 (30-F11 ...
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bioRxiv - Cell Biology 2023Quote: ... Three independent replicates of Epcam-positive kidney epithelial cells (control kidney, CK) were MAC-sorted from freshly isolated single cell suspensions from whole mouse kidneys using microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: PBMCs from three different donors were isolated from human buffy coat by Ficoll density gradient centrifugation and untouched B-cells were purified by negative magnetic enrichment using a B-cell isolation kit II (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... CD11b/c positive cells (monocyte/macrophage) were sorted using magnetic-activated cell sorting (MACS) separation columns (Miltenyi Biotec, 130-105-634) and immediately lysed with TRIzol (Fischer Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from wildtype mice spleens using CD8a+ T Cell Isolation Kit (130-104-075) from Miltenyi Biotec according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: NK cells were enriched from the spleens of C57BL/6 Kaede mice using NK Cell Isolation Kit (#130-115- 818, Miltenyi Biotec) as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: Resting B-cells were enriched from the spleen using the B-cell Isolation Kit (Miltenyi Biotec, Germany, Cat# 130-090-862). 1 mL 8 million/mL B-cells in HBSS buffer were sequentially incubated with 1 μM cholesterol strand (30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... CD34+ cells were purified from a portion of the cells using the CD34 Microbead Kit Ultrapure and LS columns (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... For two donors the B cells were isolated using MACS cell sorting with anti-human CD19 microbeads and MS columns from Miltenyi Biotec, according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... NK cells were isolated from PBMCs by negative selection using the human NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). NK cells were cultured in Roswell Park Memorial Institute medium (RPMI ...
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bioRxiv - Cell Biology 2022Quote: ... The cell solution was then filtered through a 70 μm cell strainer and separated with magnetic beads (mouse CD31, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then plated at 1-1.5×106 cells/ml with 50ng/ml human FLT3-ligand (Miltenyi Biotec; 130-096-480) and cultured for 7 days in RPMI-1640 Glutamax (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Part of the single-cell suspension was depleted of CD45+ cells to enrich for tumour cells using a CD45 MicroBeads kit (catalog no. 130-045-801; Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cell lysis was performed with ACK buffer (Thermo) before mouse cells were removed from the dissociated lungs using the mouse cell depletion kit (Miltenyi Biotec) yielding purified MeWo cells that achieved successful lung colonization ...
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bioRxiv - Cell Biology 2023Quote: ... Day 12 CD34+ hematopoietic cells were purified via magnetic-activated cell sorting (MACS) using human CD34 microbeads (#130-046-702, Miltenyi Biotec). As previously reported [48] ...
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bioRxiv - Immunology 2023Quote: ... Lineage depletion was carried out before cell sorting by MACS Cell Separation and LS columns (Miltenyi Biotec, cat#130-042-401) to deplete CD3+ ...
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bioRxiv - Immunology 2023Quote: ... Lineage-positive cells were depleted from the resulting cell suspensions using the mouse direct lineage cell depletion kit (cat. 130-110-470, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used to lyse erythrocytes from bone marrow cell suspension followed by T cells depletion using mouse CD3ε MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... were isolated using Ficoll gradient centrifugation and human T cells were obtained by magnetic sorting using the eHuman Pan T cell isolation kit (Miltenyi Biotec). Once isolated ...
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bioRxiv - Immunology 2023Quote: Autologous CD8 T cells were obtained from frozen PBMCs from the same donor using the CD8+ Microbeads human T cell isolation Kit (Miltenyi Biotec). To monitor cell proliferation ...
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bioRxiv - Systems Biology 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors and CD14+ cells were isolated using CD14 microbeads (Cat# 130-050-201, Miltenyi Biotec). THP1 cells were cultured in RPMI medium supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Systems Biology 2024Quote: ... we first processed a portion of the cell harvest solution using the dead cell removal kit (Miltenyi Biotec 130-090-101) to remove any cell debris to avoid any free RNA from lysed cells from getting mixed in with mRNA to be extracted from live cells ...
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Comparative Proteomic Study of Retinal Ganglion Cells Undergoing Various Types of Cellular StressorsbioRxiv - Molecular Biology 2023Quote: ... Cells were filtered through a 30μm cell strainer and incubated with CD90.2 magnetic beads (Miltenyi Biotec; Catalog no. 130-121-278) at 4°C for 10 minutes and then isolated using MACs LS (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Cytotoxic T cells and T helper cells were isolated from the PBMCs by positive selection with CD8- and CD4-specific MicroBeads (Miltenyi Biotec), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells, #130-092-657, Miltenyi Biotec for natural killer cells ...
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bioRxiv - Immunology 2023Quote: ... and the circulating CD45-PE+ cells were removed from the single cell suspension using magnetic sorting with anti-PE microbeads (Miltenyi Biotec) according to manufacturer’s recommendations (S1A Figure).