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Citations for Miltenyi Biotec :
1051 - 1100 of 2875 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and were cultured in RPMI medium containing 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... cells were then stained with ACSA2-PE (Miltenyi Biotec) for 30min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... were isolated by gradient centrifugation and then enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638).
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bioRxiv - Cell Biology 2022Quote: ... Purified stromal cells were then separated from epithelial cells using anti-CD326 (EpCAM) antibody-coated microbeads (Miltenyi Biotec Inc., San Diego, CA, USA). Next ...
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bioRxiv - Immunology 2019Quote: ... Cells were counted and CD8 T cells isolated using a magnetic activated cell sorting negative selection (MACS) kit according to the manufacturer’s instructions (Miltenyi Biotec, #130-104-075). 96 well plates were coated over night at 4°C with 1µg/mL anti CD3e (BD ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant was removed and dead cells were removed using the Dead Cells Removal Kit according to the manufacturer’s instruction (Miltenyi Biotec, 130-090-101). The collected effluent was spun (5 min ...
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bioRxiv - Bioengineering 2022Quote: Omicron RBD-specific memory B cells were isolated from pre-enriched memory B cells by magnetic positive selection according to the manufacturer’s protocol (Miltenyi Biotec, 130-095-838). Briefly ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... T cells were magnetically separated from other splenocytes using the Mouse Pan-T Cell Isolation Kit II (Miltenyi Biotec, Cat# 130-095-130), labeled with CellTrace™ CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2021Quote: ... Dead cell removal was performed if the viability was lower than 80% using Dead Cell Removal Kit (Miltenyi Biotec, Cat#130-090-101) as per manufacturer’s recommendations.
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bioRxiv - Neuroscience 2022Quote: ... Red Blood Cell Remover Solution was made by mixing 125 μL of Red Blood Cell Lysis Solution 10× (Miltenyi Biotec, # 130-094-183) with 1125 μL of H2O ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Cancer Biology 2022Quote: Naïve mouse CD4+ T cells were isolated from spleens of Atg5+/+ and Atg5flox/flox mice using Naïve CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Cells (5 × 105 cells in one milliliter per well ...
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bioRxiv - Immunology 2021Quote: ... PBMC were then used to isolate autologous CD3+ T cells using the MACS→ (magnetically activated cell sorting) MicroBead Isolation technique (Miltenyi Biotec, Cologne, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2021Quote: Naive CD45.1+ OT-II CD4+ T cells were isolated with MACS (Mouse naive CD4+ T cell isolation kit from Miltenyi Biotec, Bergisch Gladbach, Germany) and transferred intravenously into congenic mice (105 cell/mouse) ...
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bioRxiv - Immunology 2021Quote: Murine naïve B cells were purified from spleen by magnetic negative selection by using Pan B Cell Isolation Kit (Miltenyi Biotec, 130-095-813) according to the manufacturer’s instructions and maintained in appropriate culture medium ...
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bioRxiv - Immunology 2020Quote: PBMCs were enriched for B cells by negative selection using a pan B cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1 X Phosphate-buffered Saline (PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... After an additional 24h co-cultured cells were trypsinized and endothelial cells were purified using CD31 microbead kit (Miltenyi Biotec #130-091-935) according to manufacturer instructions.
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bioRxiv - Immunology 2022Quote: ... were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Systems Biology 2023Quote: ... we further isolated the CD34+ hematopoietic stem cells (HSCs) using magnetic-activated cell sorting according to the manufacturer’s instructions (CD34 MicroBead Kit, Miltenyi Biotec #130-046-702). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... Peripheral mononuclear cells were collected by density gradient centrifugation and enriched using a CD8+ T cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... then naïve CD4+ T cells (CD4+CD62L+CD44-) were isolated using the Miltenyi Naïve CD4+ T cell isolation kit (Miltenyi Biotec, 130-104-453), and followed by purity check by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... the negative fraction of CD8+ T cells selection was collected and incubated with the Tumor Cell Isolation Kit (Miltenyi Biotec, #130-108-339) for 15 min incubation at 4°C ...
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bioRxiv - Immunology 2023Quote: T2 and T3 cells were then depleted for CD45+ cells using the Miltenyi LD column prep kit (Miltenyi Biotec Inc., Charlestown, MA, USA). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Plasmacytoid dendritic cells were isolated by negative selection from prepared PBMCs with the Human Plasmacytoid Dendritic Cell Isolation Kit-II from Miltenyi Biotec (Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Single cell suspensions of DRG cells from BALB/cAnNRj mice were blocked with FcR blocking solution (Miltenyi Biotec l No.130-092-575) and stained with anti-CD45:PE (BioLegend 103105) ...
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bioRxiv - Cell Biology 2023Quote: PRP from human subjects was subjected to white blood cell (WBC) and red blood cell (RBC) depletion by using CD45 MicroBeads (for WBCs, #130-045-801, Miltenyi Biotec., Gaithersburg, MD) and CD235a (Glycophorin A ...
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bioRxiv - Immunology 2024Quote: ... from a cell suspension and harvested from the crushed spleens of OT-I mice using a CD8a+ T-Cell Isolation Kit and magnetic-associated cell sorting (MACS) according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). Similarly ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Lung single cell suspensions were subjected to CD4+ cell positive enrichment according to kit manufacturer’s instructions (Miltenyi Biotec, cat. no. 130-097-048) before fluorochrome staining ...
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bioRxiv - Immunology 2023Quote: ... The CD4+ T cells were then enriched by negative selection using a CD4 T cell isolation kit as per manufacturer’s instructions (Miltenyi Biotec; Bergisch Gladbach, Germany).
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples were cut into pieces of 2-4 mm and suspended using the tumor dissociation protocol with the GentleMacs® dissociator (Miltenyi Biotec GmbH, Germany). The cell suspension was applied to a cell strainer (70 μm ...
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bioRxiv - Developmental Biology 2019Quote: ... NeutrAvidin-functionalized devices were incubated with either (1) 2 μg/ml biotinylated mouse anti-human anti-SSEA-5 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany) (2 ...
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bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were cut into approximately 2 – 4mm chunks with forceps and a scalpel and transferred to a gentleMACS C tube (Miltenyi Biotec, 130-093-237) containing 4.7 ml RPMI 1640 base media ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed in MACS-buffer und T cells were MACS-sorted from suspension using a Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequently ...
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bioRxiv - Immunology 2022Quote: Primary human CD4-positive T cells were isolated from enriched peripheral blood mononuclear cell (PBMC) fractions using the human CD4+ T cell isolation kit from Miltenyi Biotec (Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Oligodendrocyte precursor cells (OPCs) were isolated from 5–7-day old YACQ84 transgenic mice using magnetic cell sorting (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany). Whole brains were dissected and homogenized using the Neural Tissue Dissociation Kit P (Miltenyi ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the separation of neurons from non-neuronal cells (astrocytes, oligodendrocytes, microglia, endothelial cells, and fibroblasts) using the Neuron Isolation Kit (Miltenyi Biotec, 130-115-389) following the kitś instructions ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533). Per 1 × 107 total cells ...