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Citations for Miltenyi Biotec :
801 - 850 of 2875 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Treg and non-Treg cells were enriched using MACS CD4+CD25+CD127dim/− Regulatory T cell isolation Kit human (Miltenyi Biotec) from PBMCs of healthy donors and plated (1 × 105 cells per well ...
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bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
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bioRxiv - Immunology 2022Quote: ... cells from lymph nodes and spleens of naïve OT-II or HNT transgenic mice were enriched for naïve cells by percoll gradients and CD4 T cells isolated by CD4 positive selection (Miltenyi Biotec) or using a CD4 naïve positive selection kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec) into complete RPMI media ...
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bioRxiv - Immunology 2023Quote: ... or CD45.2+ 4get-GFP OT-II T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec). The average purity of OT-II cells was approximately 75-80% for all experiments ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... first CD4+ T cells were isolated via positive selection using Magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). APCs were isolated with the same system via CD4 and CD3 negative selection ...
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bioRxiv - Neuroscience 2022Quote: ... hRGC differentiation was monitored by tdTomato expression and cells were purified during days 45-55 using THY1.2 microbeads and magnetic activated cell sorting system (MACS, Miltenyi Biotec) as explained before19,22 ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were strained (40 μm filters) and dead cells removed using a dead cell removal kit according to the manufacturer’s instructions (Miltenyi Biotec). Cell viability was determined using trypan blue staining and cells were counted using a haemocytometer ...
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bioRxiv - Immunology 2023Quote: ... or Panx1-KO (CD4-Cre Panx1fl/fl) CD8+ T cells were isolated from naïve mice with the Mouse CD8+ T cell isolation kit (Miltenyi Biotec). Cells were stimulated for 24h ...
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bioRxiv - Immunology 2023Quote: OT-I cells were isolated from spleen of CD90.1 OT-I mice using Naive CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturers’ guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... Pan B cells were isolated from peripheral blood leukocytes using the Pan B cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Immunology 2024Quote: T-cells were isolated from healthy donor PBMCs using the Pan T-cell Isolation Kit following manufacturer’s instructions (Miltenyi Biotec). Isolated T-cells were activated using anti-CD3/CD28 antibody-coated Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... red blood cells were eliminated using Red Blood Cell Lysis Solution (cat # 130-094-183, Miltenyi Biotec, Gaithersburg, MD, USA). These steps were followed by application of Debris Removal Solution ...
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bioRxiv - Microbiology 2023Quote: ... Enrichment of primary CD4+ T cells was performed using the EasySep™ Human CD4+ T Cell Enrichment Kit (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2023Quote: ... and B-CLL cells were further purified using the Pan B-cell Isolation Kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Versalyse buffer (Beckman-Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... CD43− B cells were enriched from the spleen by immunomagnetic depletion of non-B cells with CD43-MicroBeads (Miltenyi Biotec). The following monoclonal antibodies were used for flow-cytometric analysis of mouse lymphoid organs from 4-12 week-old mice ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from PBMC using Human CD4+ T Cell Isolation Kits (Miltenyi Biotec, Inc., Bergisch Gladbach, Germany). Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec) ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were adjusted to 5 mg/mL protein concentration and incubated for 1 h at 4 °C with 100 μL of anti-GFP magnetic beads (Miltenyi Biotec, #130-091-125). Bound proteins were analyzed by immunoblotting with anti-GFP (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Cells expressing CD45 (Miltenyi Biotec, 130-045-801), CD146 (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2021Quote: ... TAG1+ cells were isolated by MACS (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and CD8+ T cell Isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed with MACS buffer (Miltenyi Biotec) and stained with antibodies against CD40 (3/23 ...
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bioRxiv - Immunology 2022Quote: ... using magnetic-activated cell sorting (MACS, Miltenyi Biotec) according to manufacturer’s protocols and labeled with the proliferation dye CellTrace Violet (CTV) ...
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bioRxiv - Microbiology 2019Quote: ... Cells were resuspended in MACS buffer (Miltenyi Biotec) and incubated with antibodies to Ly6C conjugated to BV421 ...
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bioRxiv - Immunology 2020Quote: ... and activated with T Cell TransAct (Miltenyi Biotec); 36-48 h after activation ...
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bioRxiv - Immunology 2021Quote: ... and the magnetic cell sorter (autoMACS, Miltenyi Biotec). The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50 ...
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bioRxiv - Immunology 2020Quote: ... An additional dead cell removal step (Miltenyi Biotec) was conducted on hospitalized PBMC samples prior to aliquot for scRNA-seq ...
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bioRxiv - Microbiology 2022Quote: ... and T Cell TransAct™ human (Miltenyi Biotec) for 72 h.
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bioRxiv - Immunology 2022Quote: ... and CD4+ T cell isolation kits (Miltenyi Biotec).
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bioRxiv - Immunology 2022Quote: ... B cell kit (Miltenyi Biotec, 130-090-862) was followed according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2023Quote: ... cells were analyzed with MACSQuant VYB (Miltenyi Biotec). For experiments involving 3-ORF SEMPER ...
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bioRxiv - Cancer Biology 2023Quote: ... and a Dead Cell Removal kit (Miltenyi Biotec), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... 20 ng/mL stem cell factor (Miltenyi Biotec), 50 ng/mL vascular endothelial growth factor (GIBCO-PHC9394) ...
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bioRxiv - Microbiology 2023Quote: ... the Direct Lineage Cell Depletion Kit (Miltenyi Biotec) was employed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and Dead Cell Removal Kit (Miltenyi Biotec, Germany) if needed ...
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bioRxiv - Developmental Biology 2023Quote: ... and a MiniMACS Magnetic Cell Separator (Miltenyi Biotec). Following dead cell removal ...
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bioRxiv - Molecular Biology 2023Quote: ... and red blood cell lysis solution (Miltenyi Biotec), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... A dead cell removal kit from Miltenyi Biotec was employed after thawing ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells treated with anti-FITC MicroBeads (Miltenyi Biotec) were magnetically separated via a MACS column (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... Cells were also stained for NKG2D (Miltenyi Biotec). The PBMCs were then fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend ...
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bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of each sample was transferred to a 2 ml Eppendorf tube and the samples were assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). Green fluorescent cells were detected using the blue laser (488 nm ...
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bioRxiv - Developmental Biology 2022Quote: ... The remaining cells were pelleted and resuspended in 80 µl of MACS buffer (PBS pH 7.2, 0.5% BSA and 2 mM EDTA) and 20 µl anti-CD45 MicroBeads (Miltenyi Biotec #130-052-301) or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443 ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 80 μL of 2% BSA/PBS and incubated with 20 μL of anti-PE MicroBeads UltraPure (Miltenyi Biotec, USA.) for 15 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mM EDTA in PBS) and labeled with anti-mouse CD31-PE (1:50, #130-111-354, Miltenyi Biotec, Auburn, CA, USA), anti-mouse CD45-FITC (1:50 ...
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bioRxiv - Microbiology 2020Quote: Tissue samples were homogenized 2 × 55 sec in 1 ml sterile phosphate buffer saline (PBS) using a gentleMACS™ Octo Dissociator (Miltenyi Biotec). Total DNA from bacterial cells and infected tissues was purified using a GeneJet Genomic DNA Purification Kit (Thermo Scientific ...