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Citations for Miltenyi Biotec :
751 - 783 of 783 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Slices were cut into smaller pieces and transferred into Enzyme Mix 1 (955 μl of Buffer Z, 25 μl of Enzyme P [Miltenyi Biotec, 130-094-802]) in a 1.5-ml tube ...
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bioRxiv - Immunology 2023Quote: ... cells were spun down at 500g for 10 minutes and resuspended in MACS buffer to purify PMNs with a magnetic purification kit according to manufacturer’s instruction (Miltenyi Biotec; Neutrophil Isolation Kit, Mouse).
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Cell Biology 2022Quote: One million cells suspended in 90 μl of staining buffer (eBiosciences, 00-4222-26) were incubated with 10 μl FcBlock (Miltenyi Biotec, 130-092-575) to reduce nonspecific antibody binding ...
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bioRxiv - Immunology 2024Quote: Mouse whole blood samples were processed as follows: 200 µL of blood was incubated with 2ml of 1x Red Blood Cell Lysis buffer diluted in water (Miltenyi Biotec #130-094-183) for 10min at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell pellet was gently tapped to loosen it and then resuspended in 1 mL of wash buffer containing 50 μL of CD43 (Ly-48) MACS microbeads (Miltenyi Biotec, #130-049- 801). The mixture was incubated on ice for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... for 15 min at 4°C followed by washes and resuspension in FACS buffer (1% BSA + 1 mM EDTA in PBS) containing propidium iodide (PI; Miltenyi Biotec, 130-093-233). This cell fraction was then mixed with CountBright Plus Absolute Counting Beads (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was then minced and transferred into a 2 ml Dounce homogenizer containing nuclei extraction buffer (130-128-024, Miltenyi Biotec, Bergisch Gladbach, Germany) along with 0.2 U/ml Ribolock RNase inhibitor ...
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bioRxiv - Cancer Biology 2024Quote: Co-culture wells (96-well plates) were first washed with autoMACS rinsing buffer containing BSA (Miltenyi Biotec, #130-091-222 and #130-091-376). Cells were then treated with FcR-blocking reagent (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2024Quote: ... The dissociated cells were enriched for epithelial cells by Magnetic-activated cell sorting (MACS) (buffer: 1x PBS, 1% BSA, and 2 mM EDTA) with CD326 (EpCAM) microbeads (Miltenyi Biotec, 130-061-101) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The whole brain samples were removed and prepared into cell suspension using brain tumor dissociation kit (#130-095-942, Miltenyi Biotec, Bergisch Gladbach, Germany) and gentleMACSTM Dissociator (#130-093-235 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mM EDTA in PBS) for 15 minutes and after two washes the samples were incubated with anti-biotin-APCVio770 (Miltenyi Biotec #130-113-851), diluted 1:50 in FACS buffer for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: The tissue pieces remaining after enzymatic treatment and filtration of the fresh sample were processed using a human tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions to obtain a single-cell suspension ...
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bioRxiv - Cancer Biology 2022Quote: ... Fully de-identified human CD34 cells enriched blood samples were obtained from Bone Marrow and Transplantation Laboratory at UCSF and were MACS sorted using CD34 MicroBead Kit (Miltenyi Biotec, 130-046-702) and incubated with CD3 antibody (Biolegend ...
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bioRxiv - Developmental Biology 2023Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet from each BM sample was resuspended in 392µl PBS/3% FBS and 8µl CD117 (cKit) MicroBeads (Miltenyi Biotec, catalog number 130-091-224) and incubated on ice for 30 mins ...
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bioRxiv - Developmental Biology 2024Quote: Embryoid bodies (at least 50 EBs per sample) were pooled and dissociated using papain following the Neural Tissue Dissociation Kit – P (Miltenyi Biotec 130-092-628) and quenched with ovomucoid protease inhibitor (Worthington LK003182) ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was then resuspended with FACS buffer and subjected to α-F4/80 isolation according to the manufacturer instruction (Miltenyi Biotec, 130-110-443). After isolation ...
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bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Bioengineering 2022Quote: ... and then the cell pellet was resuspended in 100 µL of MACS buffer and 25 µL of anti-integrin α-7 microbeads (Miltenyi Biotec, #130-104-261) for incubation at 4 °C for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... cell surface-stained cells were fixed for 30 min at room temperature with Foxp3 fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5523-00 or Miltenyi Biotec, 130-093-142). After a washing step with FACS buffer ...
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bioRxiv - Genomics 2023Quote: ... the tissue was chopped into small pieces with scissors and then transferred along with the lysing buffer into a gentleMACS C tube (Miltenyi Biotec, Cat. 130-096-334). An additional 1 ml of lysing buffer was added into the C tube to make a final volume of 2 ml ...
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bioRxiv - Neuroscience 2023Quote: ... frozen brain sections were thawed in ice cold Nuclei Extraction Buffer (cat#: 130-128-024, Miltenyi) in gentleMACS™ C-Tubes (cat#: 130-093-237, Miltenyi Biotec, Bergisch Gladbach, Germany), followed by automated gentleMACS™ Octo dissociation (cat# ...
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bioRxiv - Systems Biology 2024Quote: ... Neurospheres and attached cells were resuspended in scRNA-seq buffer (HBBS + 0.04% BSA, Fisher Scientific #14-175-095 and Miltenyi Biotec #130-091-376, respectively) and washed 3 times ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were resuspended in 500 μl of MaCSQuant® Tyto® running buffer and analysed with MACSQuant® Tyto® Cell Sorter (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... We magnetically isolated all CD4+ T cells from each sample using a human CD4+ T cell negative isolation kit (Miltenyi biotec, Cat #130-096-533) per manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... liver and brain samples were homogenised in PBS (1-3 mL, adjusted to tissue weight) using gentleMACS™ M tubes (Miltenyi Biotec Ltd, Bisley, Surrey) and gentleMACS™ octo dissociator using the standard protein program ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were incubated in a water bath at 37°C for 35 min and processed using a MACS™ Dissociator (Miltenyi Biotec, 130-093-235). The homogenate was filtered through a 40 µm cell strainer ...
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bioRxiv - Genomics 2024Quote: ... an aliquot of cells per sample was centrifuged at 500 x g for 5 min at RT and used for dead cell removal (Miltenyi Biotec, ref. 130-090-101) using the autoMACS® Pro Separator following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for immunomagnetic cell separation with EpCAM-conjugated beads (Miltenyi Biotec, Catalog No. 130-105-958, 1/4). After 20 min incubation at 4°C ...
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bioRxiv - Genomics 2024Quote: ... Each fraction was washed with BSA rinsing buffer and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-allophycocyanin MACS® MicroBeads (Miltenyi Biotec, #130-090-855, lot #5190809239). Each fraction was loaded onto an MS column (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: ... and resuspended in cold selection buffer (PBS + 0.5% BSA + 2 mM EDTA) mixed with 20% (v/v) CD20 MicroBeads (Miltenyi Biotec #130-091-104 and #130-042-201) for 15 min at 4 ºC ...