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Citations for Miltenyi Biotec :
551 - 600 of 783 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: Thyroid carcinoma samples were dissociated with 30-35 ul of enzyme D Multi Tissue Dissociation Kit (Miltenyi Biotec) or 2 mg/ml collagenase IV (Gibco ...
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bioRxiv - Genomics 2024Quote: ... Fresh-frozen tissue samples were thoroughly minced on ice and placed into a gentleMACS C tube (Miltenyi Biotec) with 2ml ice-cold Hypotonic lysis buffer (10mM HEPES pH7.2 ...
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bioRxiv - Cell Biology 2023Quote: ... The magnetically labelled samples were applied to the column which was placed in a mMACS Separator (Miltenyi Biotec). The column was washed 4x with Isolation Buffer and then placed in 1.5mL tubes ...
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bioRxiv - Neuroscience 2023Quote: ... the sample and collection tubes were placed in a cold Chill5 tube rack (#130-092-951, Miltenyi Biotec), collecting both positive and negative fractions ...
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bioRxiv - Neuroscience 2023Quote: ... sample and collection tubes were placed in a cold MACS Chill 5 Rack (#130-092-951, Miltenyi Biotec) with both positive and negative fractions being collected ...
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bioRxiv - Cancer Biology 2022Quote: ... For tumor samples digestion was performed using tumor dissociation kit for human (130-095-929, MACS Miltenyi Biotec), mechanical dissociation using gentleMACSTM Dissociator running the gentleMACS program h_tumor_01 ...
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bioRxiv - Neuroscience 2023Quote: ... The microdissected sample was then transferred to a chilled gentleMACS™ C Tube (Miltenyi Biotec, Bergisch Gladbach, Germany) containing 2 ml of fresh ...
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor samples and sections were enzymatically and mechanically digested using the human Tumor Dissociation Kit (Miltenyi Biotec) to obtain single-cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: ... red blood cells were removed from the samples using RBC lysis reagent (Miltenyi Biotec, cat.no.130-094-183). After RBC lysis ...
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bioRxiv - Immunology 2024Quote: ... leukocytes were purified using magnetic-assisted cell sorting (MACS) using CD45 microbeads (35 μl per sample, Miltenyi Biotec) according to manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: ... Samples were then loaded onto µMACS columns placed in the magnetic field of a µMACS Separator (Miltenyi Biotec), washed 4 times with cold IP buffer ...
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bioRxiv - Cell Biology 2024Quote: Primary human dermal fibroblasts (hdFbs) were isolated from skin samples using the Whole Skin Dissociation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Fluorochrome-conjugated antibodies were used for specific immunostainings with dilutions (in autoMACS Running Buffer, Miltenyi Biotec, Bergisch Gladbach, Germany) as listed in table 4 ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Molecular Biology 2021Quote: ... Both the click chemistry reaction and the control sample were diluted 1:20 with SYTOX buffer (50 mM Tris-HCl pH 8.0, 0.5 µM SYTOX green) and measured on a MACSquant analyzer (Miltenyi Biotec).
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were resuspended in MACS buffer (0.5% BSA and 1 mM EDTA in PBS, pH 7.4) and incubated with CD11b MicroBeads (Miltenyi Biotec) for 30 minutes to label the microglial cells ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was removed and 1 mL of wash buffer (PBS + 0.5% BSA (Miltenyi Biotec, Cat. # 130-091-376) + 1 U/uL of RNase inhibitor ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were pelleted and resuspended in TMRE buffer and subjected to flow cytometry on a MACSQuant Analyzer (Miltenyi Biotec). For mitochondrial superoxide measurements ...
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bioRxiv - Neuroscience 2023Quote: ... enzyme mix II (enzyme A and buffer Y, from the Adult Brain Dissociation Kit #130-107-677, Miltenyi Biotec, mixed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue and digestion buffer were transferred to a gentleMACS C Tube (run protocol 37C_h_TDK_1) (Miltenyi Biotec, 130-096-334) and incubated at 37 °C for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Developmental Biology 2023Quote: ... MNCs were resuspended in ice cold CliniMACS buffer supplemented with 0.5% human albumin or AutoMACS running buffer then labelled with anti-CD34 microbeads according to manufacturer’s instructions (Miltenyi Biotec). CD34+ cells were enriched using either a CliniMACS Plus (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: ... and the cell pellet was resuspended in magnetic-activated cell sorting (MACS) buffer (Miltenyi Biotec No. 130-091-222). The tumor cells were purified on MACS columns in MACS buffer ...
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bioRxiv - Immunology 2023Quote: ... Collected PBMCs are resuspended with MACS buffer and appropriate antibodies conjugated with magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequent magnetic cell separation proceeded with a column in the magnetic field followed by a collection of unlabeled cells ...
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bioRxiv - Cell Biology 2024Quote: ... the mitochondrial fraction was resuspended in 50 μL ice-cold Storage Buffer (130-094-532, Miltenyi Biotec, CA, USA) and incubated with 312 μg/mL RNase-A (19101 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed with wash buffer and passed through an activated MS column (130-042-201, Miltenyi Biotec). The column was washed three times ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with wash buffer and then incubated with CD45 human Microbeads (130-045-801, Miltenyi Biotec) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: Dissected and trimmed tumors were washed in pre-cooled RNase-free H2O and transferred into MACS Tissue storage solution (Miltenyi Biotec, 130-100-008). Samples were shipped on ice overnight to Novogene ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were incubated with 100 μL of streptavidin-coated μMACS magnetic beads (Miltenyi Biotec, Cat# 130-074-101) for 90 minutes at 24°C under gentle agitations ...
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bioRxiv - Immunology 2021Quote: ... NK cells highly enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion kit (Miltenyi Biotec), were used as effector cells ...
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bioRxiv - Molecular Biology 2022Quote: EV samples were tested with a bead-based multiplex analysis (MACSPlex exosome kit, human, Miltenyi Biotec, Bergisch Galdbach, Germany). EV samples were diluted with MACSPlex buffer to a final volume of 120 µl with 5.8×108 EVs per sample ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor and matched normal samples were dissociated into single cell suspension using human tumor dissociation kit (Miltenyi Biotec) and gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: ... Mononuclear cells (MNC) were isolated from spleen and lung tissue samples using an OctoMACS tissue dissociation device (Miltenyi Biotec). Lung tissue samples were dissected into approximately 5mm3 pieces and incubated for one hour in a solution of 772.8 U/ml collagenase + 426 U/ml DNase (both from Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting samples were analysed by SDS-PAGE and immunoblotted using anti-GFP-HPR (Miltenyi Biotec, Bergisch Gladbach, Germany) and anti-HA-peroxidase (Roche Applied Science ...
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bioRxiv - Immunology 2022Quote: Mouse tissue samples for scRNA-seq were first positively selected for CD45+ cells using Mouse CD45 Microbeads (Miltenyi Biotec) and then sorted for 7AAD-CD45+Zbtb46-GFP+ expression before submission ...
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bioRxiv - Immunology 2022Quote: Tumor and lymph node samples were thoroughly minced with surgical scissors and transferred to GentleMACs C Tubes (Miltenyi Biotec) containing 800 U/ml Collagenase IV (Worthington ...
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bioRxiv - Cancer Biology 2024Quote: ... Depletion of CD45+ cells in NSCLC samples was performed using CD45 MicroBeads (Miltenyi Biotec, cat. no. 130-045-801) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: For single-cell sequencing experiments samples were additionally run on a gentleMACS™ Dissociator (Miltenyi Biotec, #130-093-235) with the custom program h_skin_01 (Duration ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were transferred to a plastic tube containing a knife and placed on the gentleMACS Dissociator (Miltenyi Biotec) to be further dissociated and then incubated with enzymes for 30 min at 37°C under continuous rotation ...
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bioRxiv - Bioengineering 2024Quote: All samples were blocked for non-specific binding with human FcR Blocking Reagent (Miltenyi Biotec, cat.: 130-059-901) for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail ...
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bioRxiv - Neuroscience 2023Quote: ... Analysis for plasma- and cell line-derived EV samples was performed using the MACSQuant® Analyzer 16 (Miltenyi Biotec) and the corresponding software MACSQuantify™ (Version 2.13.3) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD138+ cells were isolated from bone marrow aspirate samples using Human CD138 MicroBeads (Miltenyi Biotec, Cat # 130-051-301). Selected cells were either viably cryopreserved in 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The tumor samples were dissociated into single cells using GentleMACS tissue dissociator and its kit (Miltenyi Biotec, Gladbach, Germany). The tumor samples were cut into small pieces ...
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bioRxiv - Neuroscience 2023Quote: Whole-cleared pancreatic samples were imaged using a light-sheet fluorescence microscope (UltraMicroscope Blaze, Miltenyi Biotec, Bergisch Gladbach, Germany) equipped with a 5.5 Megapixel sCMOS camera ...
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bioRxiv - Bioengineering 2024Quote: ... 25 µl of each sample was incubated with APC-labelled CD63 antibody (Miltenyi Biotec, Germany; 1 nM per well) overnight under dark conditions ...
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bioRxiv - Genomics 2020Quote: ... 3×108 cells were resuspended in 3 ml of FACS buffer (PBS with 10% FBS) and stained with 0.9 µg anti-ter119-PE (130-102-338; Miltenyi Biotec). Stained cells were conjugated to anti-PE microbeads (130-048-801 ...
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bioRxiv - Genetics 2020Quote: ... were washed in PBS and MACS buffer (PBS, 2 µM EDTA, 0.5% BSA) and stained with Human CD34 Microbead kit (Miltenyi Biotec) following the manufacturer’s instruction for 30 minutes (4 °C ...
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bioRxiv - Immunology 2021Quote: ... Lungs from infected mice were digested using the Miltenyi lung cell isolation buffer and dissociated via GentleMACS (Miltenyi Biotec, CA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Cell suspensions (up to 1×108 cells/500 µL of Magnetic Bead Buffer) were applied onto MS columns (Miltenyi Biotec) in a magnetic field ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in PBS/0.5% BSA buffer (5 × 106 cells/ml) and purified by magnetic cell sorting using anti-O4 MicroBeads (Miltenyi Biotec) following the manufacturer’s protocol ...