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Citations for Miltenyi Biotec :
451 - 500 of 783 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and half of the cells were resuspended in binding buffer (cat# 130–090-101, Miltenyi Biotec) with dead cell removal beads ...
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bioRxiv - Systems Biology 2024Quote: ... resuspended in 400 μl of red blood cell (RBC) lysis buffer (Miltenyi Biotec #130-094-183) and incubated on ice ...
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bioRxiv - Immunology 2023Quote: ... Fluorochrome stained cells were then resuspended in sorting buffer (Miltenyi Biotec, cat. no. 130-107- 207) to achieve a concentration of 2 x 106 cells / mL of buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and resuspended in MACS buffer (Miltenyi Biotec, North Rhine-Westphalia, Germany) followed by analysis using BD FACS Canto II ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were resuspended in autoMACS buffer and labeled with anti-PSA-NCAM-APC (Miltenyi Biotec) for 10 min at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: ... Cells were then fixed and permeabilized using the FoxP3 buffer set (Miltenyi Biotec, Bergisch Gladbach, Germany). Intracellular interferon γ (IFNγ ...
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bioRxiv - Immunology 2024Quote: ... freshly isolated neutrophils were resuspended in 100μL MACS Separation Buffer (130-091-221, Miltenyi Biotec Inc.) and stained with APC-conjugated anti-human CSTA antibody (2μL/106 cells ...
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bioRxiv - Immunology 2024Quote: ... freshly isolated neutrophils were resuspended in 100μL MACS Separation Buffer (130-091-221, Miltenyi Biotec Inc.) and stained with PE-conjugated anti-human CCL4 antibody (2 μL/106 cells ...
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bioRxiv - Immunology 2024Quote: ... PBMCs cells were then resuspended by auto MACS buffer and incubated with magnetic microbeads (Miltenyi Biotec) for collecting CD3+ T cells ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were mechanically and enzymatically isolated using the Nuclei Extraction Buffer (Miltenyi Biotec, 130-128-024) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed 3 times with FACS buffer and were analysed by FACS (MACSQuant, Miltenyi Biotec). For each sample from group 1 the Geo mean fluorescence intensity (MFI ...
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bioRxiv - Cancer Biology 2024Quote: ... allophycocyanin (APC) and allophycocyanin/cyanine7 (APC/Cyanine7) in CliniMACS-buffer (130-021-201, Miltenyi Biotec GmbH) for 30 min in the dark at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... dissolved in Hank’s balanced salt solution containing 50 μg/ml DNAse I and analyzed using a MACSQuant VYB flow cytometer (Miltenyi Biotec, Germany). Sytox Blue dead cell stain (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... the index tumor lesions (the most extensive with the highest Gleason score) were immediately harvested and transferred to precooled MACS tissue storage solution (Miltenyi Biotec) and shipped at 4°C ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... A red blood cell lysis step was performed by incubating cells for 2 minutes with 1x Red Blood Cell Lysis Solution (Miltenyi Biotec). Cells were washed again and then cell viability was measured using the Nexcelom Cellometer K2 Fluorescent Viability Cell Counter and the ViaStain AOPI Staining Solution (Nexcelom) ...
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bioRxiv - Cell Biology 2021Quote: ... whole bone marrow (WBM) cells isolated by crushing femur and tibia from each mouse were incubated with 1x RBC Lysis Solution (Miltenyi Biotec) to eliminate red blood cells ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were added at a dilution of 1:100 in autoMACs Rinsing Solution (Cat. No. 130-091-222, Miltenyi Biotec) containing 0.5% BSA at 4 °C for 1 hour ...
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bioRxiv - Immunology 2021Quote: Mouse spleens and lymph nodes were removed from RNAlater solution and transferred to gentleMACS M tubes (Miltenyi Biotec 130-093-236) and 2 ml microtubes (OMNI 19-629-3 ...
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bioRxiv - Neuroscience 2023Quote: ... were sacrificed at E14.5 and brain cortices dissected under a stereomicroscope in ice-cold Hanks’ Balanced Salt Solution (HBSS) and enzymatically dissociated using a Neural Tissue Dissociation Kit (Miltenyi Biotec) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... containing enzymatic digestion solution according to manufacturer’s protocol for the Neonatal Heart Dissociation Kit (Miltenyi Biotec, Cat-Nr: 130-098-373). Two mouse hearts were processed in separate C-tubes at the same time for all experiments presented in this manuscript ...
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bioRxiv - Systems Biology 2024Quote: ... we first processed a portion of the cell harvest solution using the dead cell removal kit (Miltenyi Biotec 130-090-101) to remove any cell debris to avoid any free RNA from lysed cells from getting mixed in with mRNA to be extracted from live cells ...
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bioRxiv - Microbiology 2023Quote: ... containing 2.35 mL of a digestion solution and homogenized on a GentleMACS Octo Dissociator (Miltenyi Biotec, #130-096-427, program 37C_m_LPDK_1). Homogenates were resuspended in 5 mL PB Buffer (phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2022Quote: ... Further disaggregation of iCCA tissue into a single-cell solution for sequencing was completed using the MACS tumor dissociation kit (Miltenyi Biotec) with the standard tough tumor protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The cell solution was then filtered through a 70 μm cell strainer and separated with magnetic beads (mouse CD31, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... After dissociation the cell suspension was filtered through a 40 µm strainer and treated with red blood cell lysis solution (Miltenyi Biotec). Lastly ...
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bioRxiv - Microbiology 2024Quote: ... the mice were sacrificed and their lungs were harvested and homogenized in lysis solution (ZR Fungal/Bacterial DNA MiniPrepTM, Epigenetics) using a gentleMACS dissociator (Miltenyi Biotec). The homogenates were clarified by centrifugation and the cytokine content of the supernatant was determined using a Luminex Multiplex Assay ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were subcultivated by incubating the cells for 5 minutes at room temperature with StemMACS Passaging Solution XF (Miltenyi Biotec). Subsequently ...
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bioRxiv - Systems Biology 2020Quote: ... samples were dissociated by mechanical and enzymatic methods using the gentleMACS Tissue Dissociation Kit (Miltenyi Biotec). Resultant cell suspensions were stained with calcein violet (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: Collected tissue samples or tissue slices were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec) on gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... The dissociated breast sample was then passed through a 100 µm MACS Smart Strainers (Miltenyi Biotec), topped up with RPMI 1640 10% FCS then centrifuged at 300 x g for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: Optically cleared samples were imaged using an Ultramicroscope II Super Plan (Miltenyi Biotec, Bergisch Gladbach, Germany) equipped with 4x MI Plan Objective (NA 0.35 ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were enriched from all samples using a CD4 TIL Microbeads kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Samples were then run on the default lung_02 program on a gentleMACS Dissociator instrument (Miltenyi Biotec). Digestion was neutralized by adding 5mL 1% HI-FBS RPMI-1640 ...
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bioRxiv - Neuroscience 2023Quote: ... The column-flowthrough of the sample was then loaded on a second µ Column (Miltenyi Biotec) and residual CD63+EVs and CD81+EVs ...
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bioRxiv - Cancer Biology 2024Quote: Tissues samples were homogenized and lysed using gentleMACS Dissociators (Catalog No. 130-093-235, Miltenyi Biotec) with RIPA buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... a minimum of 1×104 events were collected per sample on a MACSQuant Analyzer (Miltenyi Biotec) and analyzed using FlowJo v.10 (Becton ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were further homogenized into single-cell suspensions with a gentleMACS™ Dissociator (Miltenyi Biotec) using a pre-set program for tumor tissues ...
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bioRxiv - Immunology 2024Quote: ... The samples were cut into smaller pieces and incubated with Tumor Dissociation Kit (Miltenyi Biotec©) according to the manufacturer’s instructions and processed using a gentleMACS™ dissociator (Miltenyi Biotec©) ...
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bioRxiv - Cancer Biology 2022Quote: ... Normal CD34+ HSPCs were enriched from thawed MPB samples using the CD34 MicroBead kit (Miltenyi Biotec). Cells were cultured in complete serum-free media (SFM ...
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bioRxiv - Systems Biology 2023Quote: ... the cell sample was filtered using Pre-Separation Filters (30 μm) (Miltenyi Biotec B.V., Leiden, Netherlands) to remove cell clumps ...
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bioRxiv - Genomics 2024Quote: ... and the samples were dissociated mechanically using a gentle MACS Dissociator (Miltenyi Biotec, San Diego, CA). All tubes were incubated at room temperature for 5 min ...
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bioRxiv - Developmental Biology 2024Quote: Cleared samples were imaged using a light-sheet UltraMicroscope II and Blaze (LaVision Biotec, Miltenyi Biotec) equipped with macrozoom (0.063 to 6x ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s instructions and analysed the samples with MACSQuant Analyzer 10 (Miltenyi Biotec, Germany). In short ...
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bioRxiv - Cancer Biology 2024Quote: ... the samples were filtered through a 70-µm MACS SmartStrainer (Miltenyi Biotec, Cat# 130-098-462) into RPMI-1640 medium (Corning ...
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bioRxiv - Genomics 2024Quote: ... uMACS columns (1x per 100ug per sample) were used to separate 4sU-labelled RNA (Miltenyi Biotec, µMACS™ Streptavidin Kit cat#130-133-282 and µ columns cat#130-042-701) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were washed with PBS and red blood cell removal was performed as described (Miltenyi Biotec). Samples were next subjected to Debris removal as described (Miltenyi Biotec) ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... The cell pellet was resuspended in MACS buffer and then applied to an MS column (Miltenyi Biotec) according to manufacture protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The remaining cell pellets were combined and re-suspended in 500μl MACS auto running buffer (Miltenyi Biotec). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... After centrifugation the single cell suspension was incubated with RBC lysis buffer (Miltenyi Biotec, #130-094-183) and incubated 2-3 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated with anti-Mouse IgG micro-bead (1:10 in autoMACS Running Buffer, Miltenyi Biotec, Germany) for 30 min at 4°C ...