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Citations for Miltenyi Biotec :
7351 - 7400 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... filtered through a 30μm cell strainer (Miltenyi Biotec cat#130-041-407) and was subsequently diluted to 1,500 cells/μl in NbActiv1 for capture on the 10x Genomics Chromium controller.
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bioRxiv - Neuroscience 2020Quote: ... To specify the differentiating cells into sensory neurons 3 µM CHIR 99021 (Miltenyi Biotec, Bergisch Gladbach, DE), 10 µM SU5402 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... single cell iPSCs were seeded at 3×105 cells/cm2 in StemMAC iPS-Brew (Miltenyi Biotec, Bergisch Gladbach, DE) in presence of 10 µM Rock-Inhibitor Y-27632 (Cell Guidance Systems ...
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bioRxiv - Genomics 2020Quote: ... Stained cells were washed with cold PBS/10% FBS and resuspended in 8 μl of cold PBS/0.5% BSA/2 mM EDTA and 2 μl of anti-PE MACS microbeads (Miltenyi Biotec: 130-048-801) per 106 cells and incubated at 4 °C for 15 minutes ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (Miltenyi Biotec: 130-102-336) at 4 °C for 20 minutes ...
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bioRxiv - Genomics 2020Quote: ... Ter119+ cells were positively selected via MACS lineage selection columns (Miltenyi Biotec: 130-042-401) and processed for downstream applications ...
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bioRxiv - Genomics 2020Quote: ... cells were incubated with anti-biotin beads (Miltenyi Biotec) for 20 min at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Magnetic columns were used to deplete non-B cells according to standard protocol (Miltenyi Biotec). Cells in effluent were further labeled with NP-PE ...
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bioRxiv - Molecular Biology 2020Quote: Immunoprecipitation of uL18L1-3HA and uL18L8-3HA were performed using the μMACS HA-Tagged Protein Isolation Kit (Miltenyi Biotec). Briefly ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The pellet was resuspended in 100μl of 1:10 dilution of FcR blocking reagent (Miltenyi Biotec) in DPBS and incubated at 4°C for 10 minutes in sterile FACS tubes ...
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bioRxiv - Immunology 2020Quote: ... purified with a CD8+ T-cell isolation kit (#130-117- 044, Miltenyi Biotec) and used as effector cells ...
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bioRxiv - Immunology 2020Quote: ... and anti-APC magnetic microbeads (#130-090-855, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: Splenic B cells of both genotypes were CD43-depleted using magnetic beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany) and taken in culture in the presence of 1μg/ml anti-CD40 antibody (102901 ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified with CD43 micro beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany), and 1×106 cells were placed in the top chamber of a transwell plate in 100μL of volume ...
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bioRxiv - Immunology 2020Quote: ... CD19 (1D3) and CD11b (M1/70) mAbs and anti-biotin microbeads (Miltenyi Biotec), followed by CD4 staining (RM4.5 ...
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bioRxiv - Immunology 2020Quote: ... Tregs were purified after enrichment of CD25+ cells using biotinylated anti-CD25 mAb (7D4) and anti-biotin microbeads (Miltenyi Biotec), followed by CD4 staining (RM4.5 ...
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bioRxiv - Immunology 2020Quote: ... CD43- splenocytes were isolated with magnetic beads (MACS, Miltenyi Biotec) and cultured (5-10 x 105 cells ml-1 ...
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bioRxiv - Immunology 2020Quote: Cryogenically preserved healthy human peripheral blood T-cells or murine splenic T-cells were magnetically isolated by negative selection using Pan T-cell isolation kits from Miltenyi Biotec. Murine splenocytes underwent RBC lysis (Sigma ...
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bioRxiv - Immunology 2020Quote: ... splenic T-cells from donor mice were enriched using the Pan T-cell Isolation Kit II (Miltenyi Biotec), while bone marrow (BM ...
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bioRxiv - Immunology 2020Quote: ... while bone marrow (BM) cells were depleted of T cells by using CD90.2 magnetic beads (Miltenyi Biotec). C57BL/6 (syngeneic) ...
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bioRxiv - Immunology 2020Quote: ... anti-Ter119 and Streptavidin Microbeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... lymphocytes were collected from spleens and lymph nodes of naïve CD45.1+ and CD45.1.2+ P14 TCR transgenic mice and negatively enriched for CD8+ T cells using the CD8a+ T Cell Isolation Kit and LS MACS Columns (Miltenyi Biotec). 1×106 CD8+ T cells were plated per well in 48 well flat-bottom plates ...
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bioRxiv - Immunology 2020Quote: ... The cells were isolated using a human negative selection-based NK isolation kit (RosetteSep, Miltenyi Biotec). The purified NK cells were then cultured in a stem cell serum-free growth medium (CellGenix GMP SCGM ...
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bioRxiv - Microbiology 2020Quote: ... using a gentleMACS dissociator (Miltenyi Biotec) on setting RNA_02.01 ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 2.4 ml of buffer RLT with 1% β-mercaptoethanol was added to the lungs from each mouse and the tissue was homogenized in an M tube (Miltenyi Biotec) using a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: Single-cell suspensions of murine melanoma cell lines were incubated with FcR blocking reagent (Miltenyi Biotec) as per supplier’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed in 1% PFA and analyzed by flow cytometry using the MACSQuant Analyzer 10x (Miltenyi Biotec).
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bioRxiv - Microbiology 2020Quote: ... antibody for 45 min at 4°C followed by the incubation with the secondary antibody anti-IgG1-PE (REA1017, Miltenyi Biotec, 1 μL/105 cells in 100 μL) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... On day 15 of cardiac differentiation fixed cells were labeled for flow cytometry using alpha-actinin (Miltenyi Biotec, Cat.#130106937) and corresponding isotype control ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with anti-CD45 magnetic beads (Miltenyi Biotec, 130-052-301) and were passed through a magnetic column ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... Supernatant was aspirated and the pellet was resuspended in 10μL Miltenyi Myelin Removal Beads II (Miltenyi Biotec Cat #: 130-096-733) and 90 μL MACS Buffer (0.5% BSA in HBSS w/o Ca2+/Mg2+ ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... Pellet was resuspended in 1mL MACS buffer and applied onto equilibrated LS Miltenyi MACS Magnetic Bead Columns (Miltenyi Biotec Cat #: 130-042-401). Columns were washed with 2mL MACS buffer and suspension was centrifuged at 300g for 5min ...
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bioRxiv - Microbiology 2020Quote: ... Mononuclear cells (MNC) were isolated from spleen and lung tissue samples using an OctoMACS tissue dissociation device (Miltenyi Biotec). Lung tissue samples were dissected into approximately 5mm3 pieces and incubated for one hour in a solution of 772.8 U/ml collagenase + 426 U/ml DNase (both from Sigma ...
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bioRxiv - Microbiology 2020Quote: ... nucleocapsid(N) or membrane glycoprotein (M) sequence (Miltenyi Biotec, Bisley, UK) or 5 μg/ml staphylococcal enterotoxin b (SEB ...
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bioRxiv - Microbiology 2020Quote: ... depleted of CD8+ T cells using a negative selection step (CD8 MicroBead Kit; LD columns, QuadMACS Separator, all from Miltenyi Biotec) per manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... For the isolation of pDCs from PBMCs the Plasmacytoid Dendritic Cell Isolation Kit II from Miltenyi Biotec (# 130-097-415) was used ...
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bioRxiv - Microbiology 2020Quote: ... and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045). The cells were washed in FACS buffer (PBS ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Microbiology 2020Quote: ... Pumorphamine 1 µM (Miltenyi Biotec, 130-104-465), Ascorbic Acid 200ng/µL ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse cells were removed with Mouse Cell Depletion kit (Miltenyi Biotec). Tumor cells were seeded 1000 cells/well in organoid medium in 384-well plates (PrimeSurface® ...
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bioRxiv - Cancer Biology 2020Quote: PDOX tumors were dissociated with the MACS Neural Dissociation kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: PDOX T434 tumors were dissociated with the MACS Neural Dissociation kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... MACS-based purification was performed with Myelin Removal Beads II followed by Mouse Cell Depletion kit (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... The isolation of lineage negative (Lin-) HSPCs or neutrophils were performed using Lineage Cell depletion kit or Neutrophil Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Single-cell suspensions were obtained by placing small sections of spleen (<5mm3) with cold phosphate buffer into gentleMACS™ C tubes and utilizing a benchtop gentleMACS™ dissociator (Miltenyi Biotec). MNCs were enriched through gradient separation (density gradient ...
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bioRxiv - Microbiology 2020Quote: ... and Fc-receptors were blocked by the mouse FcR Blocking Reagent (Miltenyi Biotec)(0.5μL/well ...
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bioRxiv - Immunology 2020Quote: Peritoneal B1a cells were obtained through negative magnetic-activated cell separation with a cocktail of antibodies depleting other than B1a cells achieving more than 90% purity in isolation (Miltenyi Biotec B.V., Utrecht, The Netherlands). B1a cells were subsequently counted and plated in IMDM medium (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... or a MACSQuant (Miltenyi Biotec) cytometers ...
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bioRxiv - Immunology 2020Quote: ... Ly6G+ cells were isolated by Neutrophil isolation Kit mouse and F4/80+ cells by Anti-F4/80 Microbeads Ultrapure mouse kit followed the manufactory instructions (MACS, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... antibiotin magnetic beads and enriched on LS columns (Miltenyi Biotec) according to manufacturer’s instructions ...