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Citations for Becton, Dickinson and Company :
4151 - 4199 of 4199 citations for Recombinant Mouse LTBR Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... LN staining: macrophages were detected using a primary antibody against CD11b (1/1000, rat anti mouse CD11b conjugated with PE; BD Biosciences, San Jose, California), B-cell follicles were identified using rat anti mouse B220 Alexa F647 (1/1000 ...
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bioRxiv - Immunology 2020Quote: IgG subclasses were measured from day 21 sera using the same ELISA protocol with the following secondary Abs: goat anti-mouse IgG1-HRP (BD Biosciences, San Jose, California), goat anti-mouse IgG2b-HRP (1:1000 ...
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bioRxiv - Immunology 2022Quote: The levels of cytokines in cell culture medium were determined with BD OptEIA™ Mouse IL-6 ELISA set (BD Biosciences, Franklin Lakes, NJ), ELISA MAX™ Standard Set Mouse IL-10 (BioLegend) ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... CD45+ cells were depleted with mouse CD45 microbeads from Miltenyi Biotech(Auburn, CA) and stained with anti-Ter119-PE (BD Biosciences, San Jose, CA), anti-CD45-FITC ...
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bioRxiv - Bioengineering 2022Quote: ... Macrophages were characterized for presence and absence of canonical markers using flow cytometry PE-Cy5 mouse anti-rat CD45 (BD Biosciences, Mississauga, ON, Canada), BV786 mouse anti-rat CD11b/c (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were washed 5x times with TBS-T and stained overnight at 4°C with gentle agitation with primary antibodies: mouse anti-rat CD63 (1:200 dilution, BD Biosciences, Mississauga, ON, Canada), rabbit anti-rat CD9 (1:500 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... double-stranded oligonucleotides encoding short hairpin RNAs (shRNAs) targeting the mouse E-cadherin mRNA were annealed and then cloned into the pSIREN-RetroQ retroviral vector (BD Biosciences; Franklin Lakes, NJ). The shRNA targeting sequence for E-cadherin was 5’-TACATCCTTCATGTGAGAGTG-3’ ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed with PBS+FBS and incubated with the monoclonal antibody anti-mouse CD8 T conjugated to Fluorescein Isothiocyanate (FITC) (BD-Pharmingen, San Diego, CA), which was suitable for determining the phenotype of CD8+ T cells ...
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bioRxiv - Bioengineering 2023Quote: Splenocytes from 125Tg B6 and WT B6 control mice were stained with CellTrace Violet and cultured for 3 days35 in complete RPMI alone or stimulated with 5 ug/mL anti-mouse anti-CD40 (BD Pharmingen clone HM40-3) in the presence or absence of conjugates described above at 150 nM concentrations ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ and CD8+ cells were isolated from spleens of RD and IF tumor bearing mice using BD-IMAG anti-mouse CD4 and CD8 magnetic particles (BD Biosciences, San Jose, CA) and were plated at a density of 7 x105 cells/well in cell-tak coated XFe 96 cell plates ...
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bioRxiv - Cancer Biology 2023Quote: CD8+ and CD4+ T-cells isolated from spleens of naïve mice using BD-IMAG anti-mouse CD4+ and CD8+ magnetic particles (BD Biosciences, San Jose, CA, USA) were cultured in 96-well plates (Nunc ...
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bioRxiv - Developmental Biology 2023Quote: ... before the cell pellet was resuspended in 100µl Fc block (mouse BD Fc Block CD16/CD32 Clone 2.4G2, Cat#: 553141, BD Pharminogen, 1:100 in FACS buffer) and incubated on ice for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min (RT) and incubated overnight (4 °C) with mouse anti-5-LOX (1:1000; # 610695; Lot: 9067817; BD Biosciences, Franklin Lakes, NJ) and rabbit anti-perilipin-2 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed with phosphate buffer three times for 10 minutes each and incubated with primary antibody (rat anti-mouse CD31, BD Biosciences, 550274, 1:100) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... blocked with 2% BSA in phosphate buffer pH=7.4 for 30min at RT and incubated with mouse anti-human LAMP1 (BD Biosciences, Franklin Lakes, NJ, USA) for 1h at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine serum albumin in PBS for 45 min at 37°C was followed by incubation with a primary antibody mix of anti-BrdU detecting IdU (mouse, #347580, BD, Franklin Lakes, NJ, USA) and anti-BrdU detecting CldU (rat ...
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bioRxiv - Immunology 2024Quote: ... according to the protocol from vendor and stained with intracellular cytokine specific antibodies Rat anti-mouse Rorγt- Brilliant Violet-650 (BD Biosciences, #564722, clone Q31-378), mouse anti-GATA3 Alexa Fluor F488 (BD Bioscience #560163 ...
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bioRxiv - Immunology 2024Quote: ... was prepared by coating the well with 50 μl of 10 μg/ml of anti-mouse IFNγ monoclonal capture antibody (BD Biosciences; cat. no. 551309) in PBS and allowed to incubate at 4°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... The ELISPOT plate was washed five times with PBST and 50 μl of 1 μg/ml of the anti-mouse IFNγ monoclonal detection antibody conjugated to biotin (BD Biosciences; cat. no. 551506) in PBS was added and allowed to incubate at room temperature for 2 hours ...
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bioRxiv - Biochemistry 2021Quote: ... The fluorescence intensity of fluorescein-labeled proteins internalized into cells was measured by flow cytometric analysis with a BD FACSantoII instrument (Becton, Dickinson and Company, NJ, USA). Approximately 104 cells were collected per specimen with three replicates.
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bioRxiv - Immunology 2019Quote: ... Serum was collected by tail-tipping before LPS injection and 2 and 4 hours following injection for IL6 determination by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufactures instructions.
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bioRxiv - Immunology 2020Quote: ... for macrophages and pDC’s were identified by co-staining of PE conjugated mouse anti-human CD123 (BD Biosciences, USA, 1:20, 37°C for 2h) and mouse anti-human HLA-DR antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... sections were incubated for 30 minutes at room temperature with a rat monoclonal antibody anti-Mouse CD8 (BD Pharmingen, BD Bioscience, San Jose, CA, USA) diluted 1:50 in blocking solution ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... ELISA was performed to measure IL-2 concentration of the thawed samples by using mouse IL-2 ELISA set (BD Biosciences, Franklin Lakes, NJ, USA) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed with PBS and incubated for 20 hours at 4°C in primary antibody solution 1:3000 mouse anti-Ki67 (BD Biosciences, San Jose, CA, USA), 2% normal horse serum ...
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bioRxiv - Immunology 2020Quote: ... Jurkat cells were maintained in culture for 1 week using enhanced RPMI and screened for TCR expression (Jing et al., 2016) using an anti-mouse TCR β chain (mTCRBC) APC antibody (BD Biosciences, San Jose, CA) and tetramer staining as described above.
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bioRxiv - Microbiology 2022Quote: ... IL-6 and IL-10 levels in cell culture supernatants were determined using the OptEIA™ mouse IL-10 ELISA kit (BD Biosciences, San Jose, CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... cell pellets were re-suspended in FACS buffer and pre-incubated with rat anti-mouse CD16/CD32 Fc Block™ (Cat# 553142, clone 2.4G2; BD Bioscience, Franklin Lakes, NJ, USA) for 10 min on ice and subsequently incubated with specific fluorochrome-conjugated monoclonal anti-mouse antibodies shown in Supplementary Table 1 ...
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bioRxiv - Immunology 2023Quote: ... and tumour necrosis factor α concentrations were quantified in lung homogenates using an ELISA kit (BD Opt EIA Mouse IL-6 ELISA Sets, #555240; BD Biosciences Franklin Lakes, NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Other antibodies and chemicals were obtained from the following manufacturers: anti-AIM-1 (AURKB) mouse monoclonal (6/AIM-1) antibodies (#611082) (BD Biosciences, Franklin Lakes, NJ, USA); anti-GFP polyclonal antibodies (#598 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice in PBS and incubated with 25 µl of an anti-mouse CD16/CD32-Fc/Block (BD Bioscience, San Jose, CA, USA) 20 minutes on ice in the dark ...
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bioRxiv - Immunology 2023Quote: ... 100 µL of whole blood was added to an antibody cocktail containing the following antibodies: mouse-anti-CD3-V500 (BD, catalog #560770, dil 1:30), mouse-anti-CD4-PerCP/Cy5.5 (BD Biosciences ...
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bioRxiv - Genomics 2023Quote: ... 2.5 × 105 cells were stained for 75 minutes at room temperature with PE Mouse anti-PDX1 Clone-658A5 (BD Biosciences; Catalog no. 562161; 1:10) and Alexa Fluor® 647 Mouse anti-NKX6.1 Clone R11-560 (BD Bioscience ...
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bioRxiv - Immunology 2024Quote: ... Supernatant from tissue lysate was diluted 1:10 and loaded onto BD Cytometric Bead Array Mouse IFNg Flex set (BD Biosciences, Franklin Lakes, NJ, USA), used as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 1:4000 polyclonal rabbit anti-cFos, Merck ABE457, lot numbers 2935662/2967893, BrdU: 1:100 monoclonal mouse anti-BrdU, BD Biosciences B44, lot number 9172603) was then applied overnight at room temperature within 3% serum in buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... after which the fraction of infected Pfeiffer cells were counted by flow cytometric analysis of the red fluorescent protein encoded by the ClonTracer cassette (BD LSRII, ex:488nm, em:575/26nm), having first gated out dead cells (Violet Viability kit ...
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bioRxiv - Cancer Biology 2019Quote: ... Positively transduced cells expressed both iRFP and ZsGreen1 fluorescent proteins and were sorted on a FACSAria™ Fusion flow cytometer (BD Biosciences, San Jose, CA, USA) to obtain a pure population of iRFP-positive cells for in vivo studies.
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bioRxiv - Molecular Biology 2022Quote: ... Viral production titers were assessed on HT1080 cells with serial dilutions of a lentivector expressing GFP and scored for green fluorescent protein (GFP) expression by flow cytometry analysis on a BD FACSVerse (BD Biosciences, Le Pont de Claix, France).
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bioRxiv - Cell Biology 2019Quote: ... at room temperature for 10 min and then incubated with Alexa Fluor® 488 Mouse anti-H2AX (pS139) antibody (clone N1-431, BD Pharmingen™, Franklin Lakes, NJ), diluted 1:1000 with 1% bovine serum albumin (BSA ...
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bioRxiv - Physiology 2020Quote: ... USA) and the lower half with a primary 42-kDa anti-β-actin antibody (chicken, ab-5, anti-mouse IgG, BD Biosciences, San Jose, CA, USA). Both antibodies were diluted 1 ...
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bioRxiv - Microbiology 2020Quote: ... enzyme-linked immunosorbent spot (ELISpot) assays were performed using the Mouse IFN-γ ELISPOT Pair kit including capture and detection antibody (BD Bioscience, Franklin Lakes, NJ, USA) and HRP Streptavidin (BD Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... Signals for 5 fluorescent markers were created by staining with antibodies against the common leukocyte antigen (Rat-anti-mouse CD45, Cat# 550539, clone: 30-F11, BD Pharmingen, San Jose, CA 95131, USA) indirectly labeled with a goat-anti-rat secondary antibody labeled with Alexa Fluor 488 ...
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bioRxiv - Biophysics 2024Quote: ... transferred in a V-bottom 96-well plates and co-cultured with K562 cells (ATCC) at 1:1 effector-to-target ratio in the presence of mouse anti-human CD107a Alexa Fluor 647 (BD Bioscience, clone H4A3, 2 uL/well) for 6 hours at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibody solutions for 5 days at 4°C: mouse monoclonal antiNMDAR1 (GluN1) (1:200; 556308, PharMingen, Becton, Dickinson and Company, Frankline Lakes, USA) or rabbit polyclonal antiNMDAa1 (GluN2A ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibody shifts were done by incubating the protein/extract with 4uL of antibody (α-Twist: Invitrogen PA5-47824, α-Snail: Santa Cruz E-18, α-Sox9: Abcam ab3667, α-E47: BD Pharmingen 554077) for 15 minutes at 25°C before adding radiolabeled probe ...
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bioRxiv - Molecular Biology 2020Quote: ... or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169; Phosphoserine/threonine clone 22A, BD Transduction Laboratories # 612548) were added to the blocking solution at dilutions ranging from 1/500 to 1,5000 according to the manufacturer’s datasheets ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by staining for 30 min at 4°C with the appropriate antibodies: PE-Cy7 rat anti-mouse CD45 (cat # 561868, BD Biosciences, clone 30-F11, 2 ng/μl), BV421 rat anti-mouse CD11b (cat # 562605 ...
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bioRxiv - Immunology 2024Quote: IL-17A and IL-10 in supernatant were quantified simultaneously using a BD™ Mouse Cytokine Cytometric Bead Array (CBA) kit and BD™ CBA FCAP array software (BD Biosciences, San Jose, CA, USA), following the manufacturer’s instructions ...