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Citations for Becton, Dickinson and Company :
4001 - 4050 of 4199 citations for Recombinant Mouse LTBR Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2021Quote: ... Intracellular proteins were stained for 60 min at room temperature after permeabilization and fixation with BD Cytofix/Cytoperm Plus (BD Biosciences, 555028) using the following antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Stable cells expressing fluorescent reporter proteins (mCherry or eGFP) were sorted using BD FACSAria Flow Cytometer (BD Bioscience, Mountain View, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Cancer Biology 2024Quote: CAR T-cells were incubated with target cells at an effector-to-target (E:T) ratio of 2:1 in complete RPMI 1640 medium containing GolgiStop™ Protein Transport Inhibitor Reagent (BD Bioscience, USA) and CD107a APC antibody (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... T cells with residual target protein expression were depleted by antibody staining and bead pull-down or cell sorting (BD Aria III). T cells were re-suspended in MACS buffer (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells expressing the relevant surface receptor or fluorescence protein were sorted using a BD FACSMelody or a BD FACSAria SORP Cell Sorter (BD Biosciences, USA). Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G ...
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bioRxiv - Cell Biology 2023Quote: ... Protein blots were visualized using Western ECL Substrate Kit (Thermo Pierce) and exposed using a ChemiDoc XRS+ system (BD, Franklin Lakes, NJ). The intensity of bands was quantified by Image J software
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bioRxiv - Immunology 2021Quote: ... Slides were treated for 10 minutes in H2O2 and after blocking in 10% goat serum in 0.1% Tween20 for 30 minutes and incubated overnight at 4°C with specific antibody diluted in PBS 0,1%Tween20: anti-mouse Ly6G (1A8, BD Pharmingen, cat 551459), or anti-mouse Ki67 (D3B5 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and incubated overnight at 4° C with primary antibodies against Syn1 (mouse, 1:1000, BD Biosciences, #610786), pSer129 (rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used for immunofluorescence of HeLa cells and/or primary neurons were: mouse anti-Ataxin-2 (BD Biosciences 611378, 1:250), mouse anti-beta-III-tubulin (Covance MMS-435P ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then plated on glass bottom dishes (No 1.5, Ø 14 mm, γ-irradiated, Martek Corporation) that had been coated with laminin (mouse, BD Biosciences) and blocked using Image-iT™ FX Signal Enhancer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Blocking was followed by 1-hour incubation with Anti-Pan Nav (Alomone labs, # ASC003) (1:50) and purified Mouse Anti-MAP II (BD biosciences, #556320) (1:400 ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were incubated with primary antibodies (rabbit anti-UPF1, Cell Signaling Technology #12040; mouse anti-G3BP1, Millipore #05-1938; rabbit anti-TIAR, BD Biosciences #610352 ...
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bioRxiv - Neuroscience 2020Quote: ... one-in-ten sections were incubated with different anti-BrdU antibodies (BrdU & CldU, rat primary antibodies at 1/200 Accurate Chemical; IdU, mouse primary antibodies at 1/200, BD Biosciences). Sections were also incubated with Zif268 (rabbit,1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed once with wash buffer 1 and incubated for two days at 4°C with mouse anti-SR antibody (1:1,000; BD Biosciences; cat #612052). Sections were washed with wash buffer 2 (2% BSA ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated for 30 minutes at 4°C in PBS containing 2% fetal bovine serum with eFluor780 viability dye (eBioscience 65-0865-18) and fluorochrome-conjugated antibodies: mouse monoclonal PE-CF594-conjugated anti-CD45 (BD Horizon 562420) and eFluor450-conjugated anti-CD3e (Thermo Fisher Scientific 48-0032-80) ...
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bioRxiv - Biochemistry 2020Quote: ... Lactate excretion in primary mouse T cells was measured using a YSI-2900 Biochemistry Analyzer after 24 hours of stimulation using anti-CD3e (BD Biosciences 553057) and anti-CD28 (BD Biosciences 553294 ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Cancer Biology 2020Quote: ... at a density of 1 million cells per 100 μL and stained with anti-mouse CD16/CD32 Fc Block™ (BD Pharmigen) for 10 min ...
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bioRxiv - Neuroscience 2019Quote: ... Drug solutions were IP injected in 0.1 ml solution/10 g of mouse using a fine needle insulin syringe (BD 324911, BD Bioscience). On average ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed twice and incubated with an R-phycoerythrin (PE)- conjugated mouse anti-human IgG1 secondary antibody (BD Biosciences, 555787) on ice for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Sections were washed and stained with primary antibody to E-cadherin (1:100 anti E-cadherin mouse monoclonal antibody, BD Biosciences 610181) and/or primary antibody to O157-antigen (1:2000 anti E ...
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bioRxiv - Microbiology 2021Quote: ... 2×106 lymph node cells were stained with the following monoclonal antibodies: rat anti-mouse CXCR5-biotin (clone 2G8, 1:100, BD, cat#551960), streptavidin PECy7 ...
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bioRxiv - Biophysics 2020Quote: Spectrin in neurons (Figure 2d and Supplementary Figures 2 and 5) was labelled with a mouse monoclonal primary antibody anti-β-Spectrin II (Clone 42/B-Spectrin II, BD Biosciences) for 1 h at room temperature using a 1:400 dilution in 5% BSA in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were also stained with mouse anti-ssDNA (DSHB University of Iowa) for 75 minutes at 37°C and detected with goat anti-mouse BV480 (BD Biosciences, 564877) for 45 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... The primary and secondary antibodies and dilutions used here were: mouse anti-BrdU 1:100 (347580; BD Biosciences, Franklin Lakes, NJ, USA), 4c4 1:200 (mouse anti-fish leukocytes ...
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bioRxiv - Cell Biology 2021Quote: γ-H2AX antibodies used were p-Histone H2A.X S139 (20E3) Rabbit mAb (Cell Signalling Technology, #9718) and Alexa Fluor 647 Mouse anti-H2AX (pS139) Clone N1-431 (BD Pharmingen, 560447).
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bioRxiv - Cancer Biology 2021Quote: ... and stained for 1 hour at 4 °C with 2 μl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti C4b (thermo fisher scientific MA1-40047,1:25) rabbit anti Olig2 (millipore AB9610, 1:250) mouse anti-Gstp (BD 610719, 1:250), anti mouse 555 (thermo fisher scientific A-21422 ...
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Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... Membranes were blocked in 5% BSA/0.1% Tween20 in 1x TBS or 5% milk/0.1% Tween20 in 1x PBS for primary antibody mouse anti-human phospho-eNOS (1:1,000, pS1177, #612392, BD Biosciences, Franklin Lakes, NJ) or mouse anti-human-eNOS (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and incubated in DMEM media containing 1% FBS and 1% BSA with Alexa Fluor® 647 Mouse Anti-Human LAMP1 Clone H4A3 (BD Biosciences) for 1h at 4°C with intermittent mixing ...
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bioRxiv - Cancer Biology 2022Quote: ... Apoptotic cells in leukemic spleens or human/mouse cell lines after different treatments were quantified with PE-AnnexinV Apoptosis Detection Kit I (BD Pharmingen, 559763). All flow cytometry data was collected on an Attune NxT Flow Cytometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... HFK were maintained in E media containing 5% FBS and 5 ng/ml of mouse epidermal growth factor (EGF, BD Biosciences; 354010) and 10 µM Rho kinase inhibitor (ROCK ...
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bioRxiv - Microbiology 2022Quote: ... HFKs were cultured in E media containing 5% FBS and 5 ng/ml of mouse epidermal growth factor (EGF, BD Biosciences; 354010) and 10 μM Rho kinase (ROCK ...
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bioRxiv - Microbiology 2022Quote: ... These cells were cultured in E media containing 5% FBS and 5 ng/ml of mouse epidermal growth factor (EGF, BD Biosciences; 354010) and with NIH 3T3 J2 fibroblast added as feeders ...
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bioRxiv - Neuroscience 2019Quote: ... mIL-6 levels were evaluated using sandwich capture ELISA assays using the RIPA soluble mouse forebrain lysates as per manufacturer’s instructions (BD OptiEIA, BD Biosciences).
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bioRxiv - Pathology 2019Quote: ... and TNF-α) in the homogenate were measured using the CBA Mouse Th1/Th2/Th17 cytokine kit (BD Biosciences; CA, United States) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Whole-mount retinas were blocked with horse serum 4°C overnight then incubated at 4°C for 3 days with primary antibodies: Mouse anti-Oct4 (1:100, BD bioscience, 611203), Rabbit anti-Sox2 (1:100 ...
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bioRxiv - Microbiology 2019Quote: ... for 1 h or 2 h and then subsequently incubated for 30 min on ice with an APC-conjugated rat anti-mouse IgG (BD Biosciences, CA). Stained cells were analyzed using a BD AccuriTM C6 Plus with a Sampler flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... slides were incubated for 50 min at 25 °C with different combinations of the following anti-mouse Abs (Thermo Fisher Scientific, Biolegend, BD Biosciences): Alexa Fluor 488-labeled anti-CD3 (HM3420 ...
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bioRxiv - Bioengineering 2019Quote: ... Isolated splenocytes were plated onto adherent culture dishes and activated with anti-mouse antibodies against CD3ε(1:1000 dilution, BD Biosciences 553058) and CD28 (1:1000 dilution ...
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bioRxiv - Immunology 2019Quote: ... Immune cells were purified by FACS isolation of CD45 positive cells using APC Mouse Anti-Human CD45 (BD Pharmingen, clone: HI30 (RUO), American ...
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bioRxiv - Biochemistry 2020Quote: ... and CD81 antibodies (with no beads) were added to the solution containing the EVs (PE Mouse Anti-Human CD81 Clone JS-81, BD Pharmingen™). After 1 h of incubation ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then blocked with 10% goat serum in staining buffer (1% bovine serum albumin (BSA) plus 0.05% Tween 20 in PBS) for one hour prior to staining with mouse anti-GM130 (BD Transduction Laboratories 610822) or mouse anti-Calreticulin (abcam ab22683 ...
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bioRxiv - Cell Biology 2019Quote: ... A second round of purification was then performed with anti-rat Dynabeads coated with rat anti-mouse ICAM-2 (rat clone 3C4; BD Biosciences #553325). Cells were cultured in Endothelial Cell Growth Medium MV2 (PromoCell ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used for the blockade of B7-1- and B7-2-dependent CD28 costimulation were anti-mouse CD80 Clone 16-10-A1 (BD Pharmingen #553736) and anti-CD86 Clone GL1 (BD Pharmingen #553689) ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody dilutions were prepared as follows in 5% NGS in 1X PBST: rat anti mouse CD11b clone M1-70 (BD Pharmingen; #553308) – 1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were allowed to dry and then hydrated and blocked for 1h at RT with 5%milk/TBS-T and incubated overnight at 4ºC with the primary antibody mouse monoclonal anti-α-Syn 1:1000 (BD Biosciences, 610787) in 5%milk/TBS-T ...