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Citations for Becton, Dickinson and Company :
4051 - 4100 of 4199 citations for Recombinant Mouse LTBR Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for 15 min at 37°C before incubation with primary antibodies Alexa Fluor 647 Rat Anti-mouse CD31 (BD Pharmigen cat:563608) for CD31 cell labeling and Alexa Fluor 647 Rat IgG2a,k Isotype control (BD Pharmigen cat:557690) ...
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bioRxiv - Cell Biology 2021Quote: Cells were treated with 25uM BrdU for 1 hour and BrdU staining detected using FITC Mouse Anti-BrdU Set (BD Biosciences, 556028). Cells were resuspended in 10μg/ml 7-Amino-Actinomycin D (7-AAD ...
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bioRxiv - Cancer Biology 2021Quote: ... The fibres were stained with primary antibodies (Rat anti-BrdU Abcam ab6326 1:250, Mouse anti-BrdU BD Biosciences 347580 1:100) overnight and with secondary antibodies (Alexafluor 555 goat anti-rat 1:500 ...
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bioRxiv - Immunology 2021Quote: ... For cell staining cells were first blocked with 1 μg/ml Fc-Block (mouse Fc Block, BD Biosciences, San Jose, CA, USA) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... a panel of four fluorescent markers was employed including fluorescein isothiocyanate (FITC) mouse anti-human CD40 (BD Biosciences; San Jose, CA, USA), phycoerythrin (PE ...
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bioRxiv - Immunology 2020Quote: ... and BV421 rat anti-mouse TNF-α BD Biosciences in a total of 100 μL of 1× Perm/Wash Buffer (BD Biosciences). After two washes ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and centrifuged at 1000rpm for 5-10min and stained with APC-Cy™7 Rat Anti-Mouse CD3 (clone: 17A2, Cat # 560590, BD Biosciences), FITC Rat Anti-Mouse CD4 (Clone ...
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bioRxiv - Immunology 2020Quote: ... The cells were blocked with 4% BSA and stained with a 1:200 (v/v) dilution of Alexa Fluor 647 Mouse anti CD247 pY142 antibody (BD Bioscience #558489) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... cells were blocked in FACS buffer containing 15% normal mouse serum for 15 min and subsequently stained for CD45 (BD biosciences, 564279), CD3 (BD biosciences ...
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bioRxiv - Immunology 2021Quote: ... before resuspending in 25 μl of 0.2µm-filtered 1% BSA/PBS containing monoclonal FITC-anti-mouse IgA (BD Pharmingen, 10 µg/ml) or Brilliant violet 421-anti-IgA (BD Pharmingen ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with goat polyclonal antibody to influenza A virus (ab20841, Abcam Inc., Toronto, ON, Canada, mouse-anti G3BP1 (BD Biosciences, 611126), Mouse anti-PABP (Santa Cruz ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-Tuj1 (gift from Tony Spano, University of Virginia, 1:250) and rat anti-Icam-II (BD Pharmingen, 553325, 1:500). Secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Pelleted cells were incubated with anti-GFP-AF647 antibody (1:150, Alexa Fluor 647 Mouse Anti-GFP Clone 1A12-6-18, BD Biosciences 565197) or rabbit anti-ITGA5 (1:100 ...
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bioRxiv - Cell Biology 2022Quote: The following antibodies were obtained commercially and used at the indicated dilutions for immunofluorescence: mouse monoclonal TOM20 (BD Biosciences #612278, 1:1000), TOMM20 (Abcam #ab186734 ...
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bioRxiv - Immunology 2022Quote: ... Transduction efficacies were determined via fluorescence activated cell sorting (FACS) staining with TCRmu antibody (anti-mouse TCR-β-chain, FITC, BD Biosciences) against the murine-β-chain of engineered TCR-constructs in comparison to non-transduced T cell populations.
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bioRxiv - Neuroscience 2023Quote: ... Concentrations of IL-1β and IL-6 were assayed according to protocol using commercially available mouse IL-1β and IL-6 ELISA (BD Bioscience, Australia). UV absorbance was quantified on a BMG PolarStar microplate reader (BMG Labtechnologies ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were blocked with 2% BSA in 0.1% tween TBS (TBST) for 1h and immunostained with mouse anti-paxillin IgG1 antibody (BD Transduction Laboratories 610052) (1:500 ...
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bioRxiv - Microbiology 2024Quote: Lung cytokines were quantified from the supernatant of thawed organ homogenates (stored at - 80°C) from each experimental timepoint using the BD Mouse Th1/Th2/Th17 Cytometric Bead Array (CBA) Kit (BD Biosciences, 560485). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used in this study: anti-total α-syn mouse monoclonal Syn-1 (1:10,000 dilution; BD Biosciences #610786), anti-PSyn rabbit monoclonal EP1536Y (1:10,000 dilution ...
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bioRxiv - Immunology 2024Quote: ... MASP-1/-3/MAP-1 mab 8B3 or F(ab’)2 fragments generated from an irrelevant mouse IgG1 mAb clone MOPC-31C (BD Biosciences, 557273) were coated on maxisorp plates at 4 °C ON and afterwards washed and blocked for 1 hr in TBS/T ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were spun at 500xG and resuspended in Flt3L DC Buffer (T-cell buffer + 10% B16-Flt3L conditioned RP10G medium + 1000U/mL Mouse GM-CSF [BD Biosciences, #554586]). Dendritic cells were plated in a total volume of 75 μL and rested overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with the following monoclonal anti-mouse antibodies as described in results: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were processed as for CDC45 but without the methanol fixation step and with a 1:1000 dilution of mouse monoclonal anti-MCM2 (BD Biosciences, 610701) for 14 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... VE-cadherin was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD144 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow from each mouse was then combined and subjected to red blood cell lysis in 1X BD PharmLyse (BD Biosciences, 555899). Cells were then washed with PBS ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Genomics 2023Quote: ... or with the appropriate class control antibodies: PE Mouse anti-IgG1 κ R-PE Clone MOPC-21 (BD Biosciences; Catalog no. 559320) and Alexa Fluor® 647 Mouse anti IgG1 κ Isotype Clone MOPC-21 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Surface staining was carried out for 30 minutes at 4°C with the following fluorochrome-labeled anti-mouse Abs: APC/Cy7-CD45 (BD Bioscience, #557659), PE/Cy5-B220 (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... followed by two washes with the permeabilization buffer before incubation with 4 μg/ml Alexa Fluor® 647 Mouse anti-Phospho-NF-κB p65 (pS529) (BD Biosciences) for 45 min.
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bioRxiv - Immunology 2024Quote: ... For the clone that binds to the lower affinity IL-2 receptor (CD122): we incubated 5ugr mouse Il-2 (PepreoTech) to 50ugr of an IL-2 mAb (S4B6; BD or BioXcell) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2024Quote: ... They were stained for 2 hours at room temp with the following primary antibodies: mouse anti-GM130 (1:250, BD Bioscience, BDB610822) and rabbit anti-TGN46 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-GluNR2B (1:1,000 for #AGC-003, Alomone lab) or mouse anti-GluN2B (1:500 for #610416, BD Transduction Lab, NJ), rabbit anti-NR2C (1:1,000 for #AGC-018 ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then incubated for 1 h with primary antibodies directed against the cis-Golgi marker GM130 (mouse anti-GM130, BD bioscience, 610822) and/or trans-Golgi marker TGN46 (sheep anti-TGN46 ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... A polyclonal population of cells expressing all 3 fluorescent proteins (mturquoise2, Venus and mCherry) was subsequently generated by two rounds of FACS (BD FACS Aria II).
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bioRxiv - Immunology 2023Quote: ... Analysis of intracellular antigens was done by using the same fixation and permeabilization as was described under “Metabolic labeling of nascent proteins” or using BD Cytofix/Cytoperm fixation/permeabilization kit (BD Biosciences, cat#554714). Maximum of 3 x 106 cells per reaction ...
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bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: Eosinophils of 6-12 week-old Il5-tg females and males were positivelyenriched using PE anti-mouse Siglec F antibody (562068 BD Biosciences; E50-2440) and anti-PE microbeads (130-042-401 Miltenyi Biotech) ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 2M HCl for 30 minutes and stained with mouse monoclonal anti-BrdU antibody (1:50) (BD Biosciences, San Jose, CA, USA) and Alexa fluor-488 conjugated secondary antibody (1:200 ...
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bioRxiv - Neuroscience 2020Quote: Western analyses were run as described[33] with the following changes: Purified mouse anti-ASyn antibody clone 42 (BD Biosciences, San Jose, CA) was diluted at 1/1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were incubated overnight at 4 °C with primary mouse monoclonal anti-alpha synuclein antibody (Cat no. 610787; BD Biosciences; 1:200) and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no ...