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Citations for Becton, Dickinson and Company :
3951 - 4000 of 4057 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The homogenized cell suspension was washed and resuspended in FACS buffer containing the PE-conjugated Rat anti-mouse F4/80 antibody (BD Horizon, Cat-565411) or eFluor450-conjugated anti-mouse CD11c antibody (eBioscience) ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were incubated with PBS/1% BSA for 30 min at 4 °C for blocking unspecific binding and washed with PBS prior to the incubation with PE-conjugated anti-human CD69 antibody (cat. 555531, BD Biosciences, BD PharMingen) for 30 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... cells were stained in a mixture of propidium iodide and anti-human antibodies anti-IgG (BV605; Clone G18–145; BD Biosciences Cat # 563246), anti-IgA (FITC ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and incubated in following antibodies for 30 minutes at 4°C in the dark: PerCP rat anti-mouse CD45 (557235, BD Biosciences, 1:50), FITC rat anti-CD11b (553310 ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with antibodies to cytokines (Table S2) and samples acquired on a flow cytometer (MACSQuant VYB, BD Fortessa or Cytek Aurora). Data was analysed in FlowJo.
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bioRxiv - Immunology 2024Quote: ... Cell suspensions were then incubated for 30 min with anti-mouse antibodies (1:500 dilution) against CD45 Brilliant Violet 711 (0.2 mg/ml; Clone 30-F11, 563709, BD Biosciences, San Jose, CA), CD45 PE-Firetm700 (0.2 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The cover slips were then permeabilized with 0.3% Triton X-100 and sequentially stained with rat anti-CD44 antibody (BD Biosciences, 550538; 1:200), and corresponding Alexa 488-conjugated secondary antibodies (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The antibodies used for stimulation were anti-human CD3 (clone UCHT1, plate bound) and anti-human CD28 (clone 28.2, BD Biosciences, San Jose, CA) at 1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed with FACS buffer stained with antibodies to detect surface expression of CD4 (GK1.5) and CD25 (PC61) (BD Biosciences, Franklin Lakes, NJ), whereas intracellular expression of FoxP3+ was stained using an anti-Mouse/Rat FoxP3 (FJK-16s ...
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bioRxiv - Biochemistry 2024Quote: ... Antibody-stained cells were washed in FACS staining buffer and resuspended in 20 µL 7-actinomycin D (7-AAD, BD Pharmigen flow kit) for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... blood from non-tumor bearing mice) were positively enriched for eosinophils using a PE anti-mouse Siglec-F antibody (562068, BD Biosciences; E50-2440) and anti-PE microbeads (130-042-401 ...
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bioRxiv - Genetics 2024Quote: ... Cells were stained with 5 ml PE Mouse Anti-Cardiac Troponin T antibody (564767, clone 13–11, BD Biosciences, San Jose, CA, USA) for 45 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... A master mix (5 µL of each fluorochrome-conjugated antibody + 10 µL of Brilliant Staining Buffer (Figure 1 a & b) (BD Horizon™ #659611) or Super Bright Complete Staining Buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... cell samples were stained with the following antibodies in cell staining buffer for 40 min at 4 °C: anti-mouse-CD3e-FITC (553061, BD Biosciences, 1:200), anti-mouse-CD4-PercpCy5.5 (550954 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were dissociated and stained with an A647- conjugated antibody against the target gene product CD9 (#341648, BD Biosciences, Franklin Lakes, NJ, USA). Cells were suspended in PBS and FACS sorting was done using a BD Influx (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: EMA-labeled DNA was added to MM cells and incubated overnight followed by staining with anti-human CD138-FITC antibody (BD, cat no. 552723). Presence of DNA was determined using an ImageStreamX Mark II instrument (Amnis Corporation ...
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bioRxiv - Molecular Biology 2021Quote: ... stained with a collection of different fluorescent labelled antibodies (CD4-BV785; BioLegend, San Diego, CA, USA; CD25-PE-Cy5.5; BD Bioscience; and CD54-AF700; EXBIO) and analysed on a Cytoflex flow cytometer (Software CytExpert 2.3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Enumeration of human CD45+ cells in spleen and bone marrow was performed by flow cytometry after staining with BV605-conjugated human-specific CD45 antibody (clone HI30, BD Biosciences, San Jose, CA) using a BD LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... The DCs were phenotyped using phycoerythrin-conjugated (PE) monoclonal antibodies (mAbs) against CD83 and CD14 setting the cutoff for positive cells with the isotype control IgG2a (BD Pharmingen, Franklin Lakes, NJ). Immature DCs were transferred to new plates at a concentration of 4×105cells/mL ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... Synaptic ribbons were labeled with anti-CtBP2 (1/200 Goat polyclonal, Santa Cruz, USA ; cat # SC-5966 or 1/200 Mouse IgG1 monoclonal antibody BD Biosciences, cat # 612044). Otoferlin was labeled with a mouse monoclonal antibody (1/200 AbCam ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 4% milk for 1 hour and incubated overnight at 4°C with shaking in the appropriate primary antibody against Nischarin (1:500, BD Biosciences, Cat No: 558262), GFP (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Cochlear whole mounts were immunostained overnight at 4°C with primary antibody against CTBP2 (mouse anti-CTBP2 at 1:200; BD Transduction Labs, Heidelberg, Germany). Secondary antibody (donkey anti mouse conjugated with Cy3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated overnight at 4°C in solution containing 1% BSA and primary antibodies: mouse F4/80 (T45-2342, BD Biosciences, New Jersey, USA) 1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were washed three times with PBS and then incubated with primary antibodies anti-ASO (Ionis Pharmaceuticals. Carlsbad, CA) and anti-CD31 (BD Bioscience, San Jose, CA) with a 1:200 dilution in blocking buffer at 4 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated as described above and stained at 4°C (15 min) with fluorochrome-conjugated antibodies against surface markers of endothelial cells (CD31 (BD Biosciences, Franklin Lakes, USA) and myeloid cells (CD45 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: qNSCs and aNSCs from the SVZ were FACS purified from GFAP::GFP mice as previously described (Codega et al., 2014) using the following antibodies: anti-mCD24 PE (1:2000; BD Pharmingen 12-0242-82), biotin conjugated anti-mCD133 (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with Alexa Fluor 647–conjugated anti– human CD24 and APC-H7 labeled anti–human CD44 antibodies (BD Pharmingen, San Jose, CA) at 4°C for 20 min ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Immunology 2020Quote: ... The following primary antibodies were used: AF-488 anti-rat CD3 (Biolegend. Clone 1F4, PE-Cy5 Mouse anti-rat CD4 (BD bioscience. Clone Ox-35), PE Mouse anti-rat CD8α (BD bioscience ...
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bioRxiv - Bioengineering 2021Quote: ... cells were stained with surface marker antibodies and analyzed with Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Propidium Iodide (5:1000 µL ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Physiology 2021Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...
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bioRxiv - Cancer Biology 2021Quote: ... Negative cells were sorted for the absence of PD-L1 by staining with a PE-labeled anti-mouse PD-L1 antibody (clone MIH5, BD Biosciences, Franklin Lakes, NJ) using a FACSAria III (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... harvested and stained for flow cytometry as described (37) using antibodies to human surface antigens: CD4-APCH7 (SK3, BD Biosciences Franklin Lakes, NJ, USA), CD8-PerCP (SK1 ...
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bioRxiv - Immunology 2020Quote: ... The 3T3 cells were FACS sorted four times for expression of CD40L using an anti-CD40L antibody (clone TRAP1, BD Bioscience, San Jose, California). This resulted in a stable CD40L-expressing cell line that was cultivated in G418 containing selection media to maintain expression ...
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bioRxiv - Immunology 2020Quote: ... LN staining: macrophages were detected using a primary antibody against CD11b (1/1000, rat anti mouse CD11b conjugated with PE; BD Biosciences, San Jose, California), B-cell follicles were identified using rat anti mouse B220 Alexa F647 (1/1000 ...
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bioRxiv - Cancer Biology 2023Quote: Ba/F3 cells expressing human MPL protein that were either cultured normally or treated with drugs were harvested and stained for surface MPL using PE-conjugated anti-human MPL antibody (BD Pharmingen 562159,1:50 dilution) in staining buffer for 30 min at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... 1x106 cells in 100 μL were stained for 30 minutes at room temperature using the following antibodies: CD90-BUV395 (BD, #740260, 0.1 mg/μL), ECAD-AF405 (Novus Bio ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluoresceinated antibodies against (TCRý, CD4, CD8, CD3, CD3χ, CXCR5, PD1, and intracellularly for ZAP70 and IL-10) (BD Biosciences, San Jose, CA) and data acquired on a FORTESSA flow cytometer and analyzed using FlowJo Software (Ashland ...
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bioRxiv - Genetics 2023Quote: ... USA)/anti-rabbit IgG Alexa 488 (Jackson Immuno Research lab, West Grove, PA, USA) and 10µg/mL biotinylated anti-CD45 antibodies (BD Bioscience, Franklin Lakes, NJ, USA)/anti Rat IgG Alexa Plus 647 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... 1.5 µg/mL) [31] in the presence of anti-CD28 and anti-CD49d antibodies (both at 1 μg/ml, BD, Franklin Lakes, New Jersey) and brefeldin-A (10 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with primary antibodies for at least 1 hr at room temperature and secondary antibodies (for nonconjugated primary antibodies) for 30 min at room temperature with washes between the steps before analysis by flow cytometry (BD Fortessa or MACSQuant 16). To exclude dead cells ...
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bioRxiv - Cell Biology 2024Quote: ... upper membrane parts were first probed with rat monoclonal anti-pSMAD1/9 antibody (pS463/pS465, pS465/pS467, 1:1,000, #562508, BD Biosciences, East Rutherford, NJ, USA), or rabbit monoclonal anti-pSMAD2 (S465/467 ...
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bioRxiv - Developmental Biology 2024Quote: ... Animal caps were blocked for 1 hour using sheep serum and bovine serum albumin and then incubated in 1:250 anti-β-catenin antibody (BD Bioscience cat# 610154) in blocking solution overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The samples included for T cell phenotypic characterization were stained with a separate antibody cocktail containing T cell lineage markers (CD3-BV786 (BD, cat. #563799, clone SK7), CD4-BV650 (BD ...