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Citations for Becton, Dickinson and Company :
3601 - 3650 of 4057 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Differentiation was confirmed by measuring expression of CD11b and CD14 markers by flow cytometry (antibodies: CD11b, clone D12; CD14, clone M5E2, BD Pharmingen).
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1× perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used on cells isolated from bleeds and spleen/bone marrow of NSG treated mice: CD3 APC R700 (BD 565119), CD4 BB700 (BD 566392) ...
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bioRxiv - Immunology 2023Quote: ... Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution, clone R2-40, BD Bioscience) for 30 min on ice ...
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bioRxiv - Microbiology 2023Quote: ... Human erythrocyte engraftment was assayed by flow cytometry of peripheral blood incubated with APC-conjugated TER-119 antimouse monoclonal antibody (BD Biosciences), with gates at APC+ and APC- (mouse and human erythrocytes ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs were stained with a panel of fluorochrome-conjugated antibodies reactive with the following cell surface markers: CD45-APC/Cy7 (clone 30-F11; BD Biosciences), CD3-PE/Cy7 (clone 500A2 ...
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bioRxiv - Immunology 2023Quote: ... with the same panel of human antibodies as used for cell sorting and analyzed by flow cytometry on a FACS Fortessa (BD Biosciences). UltraComp eBeads (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by two PBS-wash steps and secondary antibody staining and analysis on an LSRFortessa X-20 flow cytometer (BD Biosciences), which was also used for all downstream flow cytometry.
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bioRxiv - Cancer Biology 2023Quote: ... tumor or normal organ tissue slides were incubated with primary antibody of Ki-67 (1:10000 dilution; Cat# 550609, BD Biosciences), p-Abi-1-S323 (1:500 dilution ...
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bioRxiv - Pathology 2023Quote: ... then incubated for 20 minutes at 4°C with a combination of the following mouse anti-human antibodies: AF488 anti-CD3 (clone UCHT1, BD Biosciences), PE anti-CD203c (clone NP4D6 ...
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bioRxiv - Bioengineering 2023Quote: The following primary antibodies were used as previously demonstrated in MDCK cells: purified mouse anti-p120 catenin 56 (BD Biosciences, 610133) at a dilution of 1:200 and recombinant rabbit monoclonal anti-vinculin 35 (abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated with an intracellular antibody panel for 30 min at RT in 1x permeability/wash buffer (BD #554723), including NeuN-APC (1:50) ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was discarded and the pellet was resuspended in Staining buffer containing the Human BD Fc Block™ antibody (BD, 564220) and incubated with agitation for 15 min at 4°C in a cold room ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained for 20 min a 4°C with the appropriate combinations of fluorochrome-conjugated anti-human antibodies for the identification of CD8 T cells and sub-populations: CD3 BV480 (clone UCHT1, BD Biosciences), CD8 BV421 (clone RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... purified NK cells were incubated for 10 minutes at room temperature with 2.5µg of Human BD Fc Block followed by addition of 10μg/mL of anti-NKG2D purified antibody (BD Biosciences, clone 1D11) or isotype control antibodies prior to addition of SVG-A cells ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequently hLSECs were purified via FACS by labelling with mouse anti-human CD31-FITC conjugated antibody (clone WM-59, BD Biosciences). Two independent preparations of transplanted iEC were used for single cell RNAseq ...
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bioRxiv - Bioengineering 2023Quote: ... At the end of the co-culture the cells were washed twice and incubated with human or mouse Fc receptor blocking antibodies (BD Biosciences). Human macrophages were stained with anti-CD11b (M1/70 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then washed and stained using anti-mouse monoclonal antibodies: CD45.1 Brilliant Violet 510 (clone A20, BD Pharmingen, RRID: AB_2739150), CD45.2 FITC (clone 104 ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Cell Biology 2023Quote: ... PECAM was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD31 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Immunology 2023Quote: ... and the 2D effective affinity AcKa was estimated by normalizing
against mr and ml that were measured using PE-labeled monoclonal antibody together with QuantiBRITE PE standard beads (BD Biosciences): -
bioRxiv - Immunology 2023Quote: ... Blue staining of PCNA was achieved by using a species-cross reactive mouse IgG2a-kappa monoclonal antibody directed against PCNA (clone PC10; BD Bioscience) and the Vector® Blue Substrate Kit ...
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Immunology 2023Quote: ... and CD4+FYP+ Treg cells at indicated Treg : Tresp ratios in the presence of 1 μg/ml anti-CD3 antibody (BD Pharmingen). After 3 days ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 300 µg/mL DNase for 1 hour at 37°C to expose incorporated brdU and then stained with an APC anti-brdU antibody (1:50, BD Biosciences). 1X BD Perm/Wash in ddH2O was used as a staining and washing buffer ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with serum (2 fold dilutions, 1:20-1:2560) and parasite specific antibody was measured using biotinylated IgG1 (BD Biosciences) or IgG2c (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were then permeabilized with BD Fix/Perm for 20 min at room temperature (RT) and stained intracellularly with antibodies in 1x Perm buffer (BD Biosciences): anti-IFN-γ (1:100 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then resuspended in BD Perm/wash buffer with the following antibodies: BV650 IL4 (1:50, BD Bioscience, cat#564004), APC-Fire750 IFNγ (1:80 ...
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bioRxiv - Physiology 2022Quote: ... Novus) and F(ab’)2-donkey anti-Rabbit IgG (H+L) secondary antibody PE (1:100, 12-4739-81, BD bioscience) at 4℃ for 1h ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were stained with the antibody cocktail in the staining buffer of a 1:1 mix of PBS and Brilliant Staining Buffer (BD, 566349), at room temperature for 20 mins or at 4°C for 30-40 mins ...
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bioRxiv - Immunology 2022Quote: Single-cell suspensions from colonic lamina propria were stained with fluorescently labelled antibodies and isolated using a BD FACSAria III cell sorter (BD Biosciences). Live CD45+ Lin- CD127+ ILC FACS sorted as KLRG1+ and KLRG1- were cultured in DMEM supplemented with 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... Cell subsets for the populations from different donors were labelled simultaneously with the cell surface sorting panel (Table S4) an oligonucleotide-tagged antibody sample tag (BD Biosciences) prior to cell sorting on a FACSAria3 (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... CD137 expression on CD8+ T cells was assessed after an overnight co-culture with an anti-CD137 PE antibody (BD Biosciences).
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bioRxiv - Molecular Biology 2022Quote: ... The CldU and IdU tracks were detected by IF with anti BrdU antibodies (rat for CldU, Abcam ab6326, and mouse for IdU, BD 347580). Fiber integrity was assessed with a mouse anti-ssDNA antibody (Millipore MAB3034) ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-β2 mAb IB4 (10 μg/ml) followed by Alexa Fluor 488-conjugated secondary antibody and analyzed using a FACScan (BD Biosciences) and Attune NxT flow cytometer (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... sorted T3a and T3b B cells were assayed for differentiation to FO at 24 hours in culture by flow cytometry using MitoTracker staining in conjunction with the following primary antibody panel: CD38 (HIT2, BD Biosciences), CD24 (ML5 ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... and stained for 30 min on ice with surface antibodies at a 1:200 dilution in a 1:1 mixture of FACS buffer and Brilliant Stain Buffer (BD Biosciences). Cells were washed with FACS buffer and PBS and fixed with 2% paraformaldehyde on ice for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µg of this genomic DNA from soluble fraction was mixed with 3 µg of anti-IdU/BrdU antibody (3D4 clone, BD Science) in BrdU-IP buffer composed of PBS and 0.0625% Triton X-100 overnight ...
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bioRxiv - Microbiology 2023Quote: ... for 16 hours followed by stimulation with a peptide pool of a broad range of HLA-subtypes and infectious agents (CEFX Ultra SuperStim Pool; JPT) (1 µg/ml) and 1 μg/ml mouse-anti-human CD28 antibody (#556620; BD Biosciences), or IL-2 (20 IU/ml ...
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bioRxiv - Immunology 2024Quote: MNCs from either IP or peak were immunophenotyped by FACS using the monoclonal antibodies CD3-FITC (BD Biosciences, San Jose, CA), CD4-FITC (BD Biosciences) ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were blocked with 2% normal mouse serum and an anti-CD16/CD32 antibody (FcγR blocker, BD Pharmingen™ 553142) for 30 min on ice ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were stained with Alexa Fluor 647-conjugated rat anti-CD49f antibody (5% v/v in a 100 µL reaction; BD Biosciences) for 25 minutes at 37°C on an orbital shaker at 200 rcf and resuspended in DPBS containing 0.5% bovine serum albumin and 50 units/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... cells were counted then stained with optimal concentrations of the following fluorophore-antibody conjugations: BB700 anti-IgG1 (clone A85-1, BD Biosciences), BB700 anti-IgG2a (clone R2-40 ...
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bioRxiv - Microbiology 2024Quote: ... The antibodies employed for ELISA were biotin-conjugated rat anti-mouse IgG2a (clone R19-5, BD Biosciences, Denmark cat no. 550332) and anti-mouse IgG conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated with anti-CD16/32 APC-Fire750 and anti-CD64 PECy7 for 15 min on ice before the addition of the rest of the fluorophore-conjugated antibodies were added on ice for 20 min in Brilliant Stain Buffer (BD Biosciences). For GATA6 staining ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were then washed with FACS buffer and placed on ice for incubation with anti-isotype detection antibodies depending on application: anti-IgG3-BV421 (R40-82, BD Bioscience), anti-IgM-PE/Cy7 (RMM-1 ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated overnight with hamster anti-CD3ε antibody diluted in 1% goat serum/0.3% Triton-X100/TBS (BD Biosciences, 550277). The next day samples were washed in TBS and incubated at room temperature in goat anti-hamster 647 (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell death was identified by analysis of cells stained with annexin V-(AnV-FITC) and propidium iodide (PI) antibodies before flow cytometry analysis as recommended (BD Biosciences).