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Citations for Becton, Dickinson and Company :
3851 - 3900 of 4057 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The cells were then stained at a 1:50 dilution with antibodies to immune cell targets as follows: CD3- FITC (BD Biosciences, 555332), CD8-PE (BD Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with the following monoclonal anti-mouse antibodies as described in results: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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bioRxiv - Immunology 2023Quote: ... For the negative enrichment we used the same antibodies as for the staining but PE-labeled (Biolegend, Cat. 328109, 334105, 303105, 313203, 343605, 304058, 337004 and 305817; BD, Cat. 561433). After negative enrichment ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Immunology 2023Quote: The Antibody mix was supplemented at 10 uL with BD Horizon™ Brilliant Stain Buffer Plus (BD Biosciences, Franklin Lakes, NJ, USA) to minimize staining artifacts commonly observed when several BD Horizon Brilliant dyes are used ...
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Genomics 2023Quote: ... or with the appropriate class control antibodies: PE Mouse anti-IgG1 κ R-PE Clone MOPC-21 (BD Biosciences; Catalog no. 559320) and Alexa Fluor® 647 Mouse anti IgG1 κ Isotype Clone MOPC-21 (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in this study: rabbit anti-AP-4 epsilon 1:400 for Western blots (612019; BD Transduction Labs); rabbit anti-ATG9A 1:200 for immunofluorescence and 1:1000 for Western blots (ab108338 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated with anti-mouse CD32 for 5 min at 4°C to block Fc receptors and stained with fluorochrome conjugated antibodies to CD45R/B220 PE (1:200) (clone RA3-6B2, BD Bioscience #553089), Ter119 PE (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... Content of chemokines / cytokines was quantified after washing and staining with biotinylated detection antibodies and phycoerythrin bound streptavidin by using LSR II (BD Bioscience, Germany). Calculation of each chemokine / cytokine quantity was determined by using standard curves according manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: Intracellular staining for BIM was performed with secondary Donkey anti-rabbit APC secondary antibody using BD cytofix/cytoperm kit (BD biosciences 554722) per manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... The cells were then incubated for 1 h with primary antibodies directed against the cis-Golgi marker GM130 (mouse anti-GM130, BD bioscience, 610822) and/or trans-Golgi marker TGN46 (sheep anti-TGN46 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed and then stained extracellularly with antibodies (Table S1) diluted in 0.5% FACS buffer and brilliant staining buffer (BD Biosciences, Cat# 563794) for 20 mins at room temp ...
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bioRxiv - Immunology 2024Quote: ... three LNs (for patients ccp2 and ccp3) and two replicates of PBMC were stained with a panel of fluorescent antibodies: CD45-PerCP/Cy5.5 (BD 564105, clone HI30), CD38-PE (BD 555460 ...
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bioRxiv - Cell Biology 2024Quote: ... They were stained for 2 hours at room temp with the following primary antibodies: mouse anti-GM130 (1:250, BD Bioscience, BDB610822) and rabbit anti-TGN46 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... cells were suspended in 0.5 mL PBS+3% FBS and stained for flow cytometry analysis by adding anti-CD16/CD32 antibody (BD Pharmingen; clone 2.4G2) ,anti- CD117 (Biolegend clone 2B8) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were blocked with Superblock blocking buffer in PBS/ 0.1% Triton-X for 1 h and then incubated with the following primary antibodies overnight at 4 °C: α-CD11b (1:100; BD Biosciences) and α-Iba1 (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... The Fc cell receptors were then blocked for 10 min at 4 °C with and anti-CD16/anti-CD32 antibodies (10 µg/mL: BD Biosciences 553142) and the cells were resuspended in 25 µL of staining buffer ...
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bioRxiv - Immunology 2024Quote: ... in PBS for 1 hour at room temperature followed by overnight incubation at 4°C with 1:500 Ly6G&6C antibody (BD Biosciences, 550291) in 2% normal goat serum in PBS overnight ...
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bioRxiv - Immunology 2024Quote: ... Cells were then fixed and stained with a combination of fluorochrome-conjugated antibodies using Cytofix/Cytoperm Fixation/Permeabilization Buffer (BD Biosciences, U.S.A.) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C with one of the following primary antibodies: rat monoclonal mouse anti-BrdU/IdU (1:250 or 1:500, BdBiosciences B44/347580), rat monoclonal anti-BrdU/CldU (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were incubated for one hour at RT in a humid chamber with a 1X PBG mix containing an anti-BrdU primary antibody (BD Laboratories, #347580) diluted 1:20 and RQ1 DNAse (Promega ...
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bioRxiv - Molecular Biology 2024Quote: We performed extracellular staining using the following antibodies (dilution, clone, company, catalog number): anti-mouse CD45.2 BUV395 (1:100, 104, BD Biosciences, Cat. 564616), anti-mouse CD3 BUV737 (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... followed by 1:25000 donkey anti-human HRP-conjugated secondary antibody (Jackson Immuno Research Laboratories 709-035-149) for 30 min and detection with TMB SureBlue peroxidase substrate (BD Biosciences 555214) was measured at OD450nm.
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bioRxiv - Immunology 2024Quote: ... Cells were stained in permeabilization buffer with the indicated antibodies and analyzed on a FACSCanto II or FACSymphony A1 (BD, Mississauga, Ontario). Graphs were prepared using FlowJo.
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bioRxiv - Immunology 2024Quote: ... containing approximately 105 human CD34+ hematopoietic stem cells (HSC) as determined by FACS using a mouse anti-human CD34 antibody (BD Biosciences, 550761). CD3 depletion of human T cells was required to avoid lethal (acute ...
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bioRxiv - Systems Biology 2024Quote: ... 1:200 diluted human Actinin(Sarcomeric)-PE (Miltenyi, 130-123-996) and 1:400 diluted mouse Aurora B primary antibody (BD Bioscience, 611082) were added at 4°C in the dark for 16–24 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed with FACS staining buffer and incubated with α-BrdU antibody conjugated to APC (1:50, BD Pharmigen flow kit) and α-pHH3 (Ser10 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated with AF647-anti-VE-cadherin antibody (1:200, v/v, diluted in pre-warmed EGM-2 media, BD Biosciences, #561567) for 30 minutes orbital shaking ...
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bioRxiv - Bioengineering 2024Quote: NOD8.3 CD8 T cells were isolated from splenoyctes following manufacturer’s protocol (Miltenyi Biotech 130-104-075) and αCD3/αCD28 antibody (BD Biosciences 553057/ 553294) activated for 48 hours ...
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bioRxiv - Cancer Biology 2024Quote: High-affinity microplates were sensitized with monoclonal anti-cytokine antibodies (IL-6: 555220; IL-10: 555157; TNF-α: 555212; IL-1β: 557953, OptEIA, BD Biosciences) for 16-18h ...
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bioRxiv - Cancer Biology 2024Quote: ... plates were coated with anti-β1-integrin antibody mix (5 μg/ml of AIIB2 (in-house) and anti-CD29 (BD Bioscience #610468)) in TBS (50 μl per well ...
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bioRxiv - Cancer Biology 2024Quote: ... To probe IdU, the samples were incubated with mouse anti-IdU antibody (Abcam, ab6326; 1:25 in blocking buffer (BD Bioscience, 347580)) for 60-minutes each at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... PE mouse anti-human VCAM-1 or Alexa Fluor 647 mouse anti-human CD31 (all antibodies were from BD, Franklin Lakes, NJ, USA). Samples were analyzed on Gallios Flow Cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibodies used for the flow cytometry analysis of the cell surface markers were: anti-human CD11b (740965, BD OptiBuild™, 1:100), anti-human CD14 (11-0149-42 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: Eosinophils of 6-12 week-old Il5-tg females and males were positivelyenriched using PE anti-mouse Siglec F antibody (562068 BD Biosciences; E50-2440) and anti-PE microbeads (130-042-401 Miltenyi Biotech) ...
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bioRxiv - Immunology 2021Quote: ... Zap70 and CD49d were evaluated by flow cytometry (antibodies in Table S2) on an LSR II flow cytometer (BD Biosciences, Franklin Lakes, NJ). Mutational status of the expressed BCR was determined by PCR from PBMC RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
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bioRxiv - Molecular Biology 2020Quote: ... Edited and non-edited cells were analyzed by flow cytometry using the following antibodies: hCD45 V450 (HI30; BD Biosciences, San Jose, CA, USA), CD34 APC (561 ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for ON in primary antibody (SOX2, R&D Systems, MAB2018; TBR2, Abcam, ab23345; HOPX, Santa Cruz, sc398703; HOPX, Proteintech, 11419-1-AP; N-cadherin, BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 2M HCl for 30 minutes and stained with mouse monoclonal anti-BrdU antibody (1:50) (BD Biosciences, San Jose, CA, USA) and Alexa fluor-488 conjugated secondary antibody (1:200 ...
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bioRxiv - Neuroscience 2020Quote: Western analyses were run as described[33] with the following changes: Purified mouse anti-ASyn antibody clone 42 (BD Biosciences, San Jose, CA) was diluted at 1/1000 ...
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bioRxiv - Cell Biology 2021Quote: ... washed with blocking buffer and incubated with fluorochrome-conjugated antibodies (1:100 dilution in blocking buffer) against syndecan-1 (BD Biosciences Cat#: 565943), syndecan-2 (R&D Systems Cat# ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked in PBS + 5% BSA for 1 hour before addition of primary antibodies (anti-LC3A/B, CST #4108, 1:100; anti-LAMP1, BD #555798, 1:100) diluted in blocking buffer overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were incubated overnight at 4 °C with primary mouse monoclonal anti-alpha synuclein antibody (Cat no. 610787; BD Biosciences; 1:200) and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no ...
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bioRxiv - Neuroscience 2020Quote: ... astrocytes were first starved (DMEM without serum) for one hour and subsequently incubated with the 9EG7 antibody (BD Bioscience, 1:20 in DMEM) on ice for 1 h ...