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Citations for Becton, Dickinson and Company :
3001 - 3050 of 3331 citations for 8 4 dimethylaminophenyl diazenyl N N dimethyl 10 phenylphenazin 10 ium 2 amine chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: OT-I cells were expanded for 5 days in the presence of IL-2 after transduction and sorted on Thy-1.1 surface expression on a BD FACSAria Fusion cell sorter (BD Biosciences) directly into RLT Plus lysis buffer (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... at a concentration of 1 x 106 cells/mL with RPMI plus 2 μL/mL Leukocyte Activation Cocktail with GolgiPlug (BD) and incubated for 4 hours at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... cells were pelleted and then resuspended in 2 mL of 0.5% BSA in D-PBS for cytometer-based sorting (FACSAria IIIu cell sorter, BD Biosciences). An aliquot of 200 µL stained cells was saved as input for analysis ...
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bioRxiv - Immunology 2021Quote: ... followed by FcR blockage with mouse serum (2%) and FcR blocking anti-mouse CD16/32 antibodies (clone 2.4G2, BD Biosciences). Next ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed twice in PBS with 0.5% BSA and 2 mM EDTA and run on LSR II flow cytometer (BD Biosciences). Data were analyzed using FlowJo software ...
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bioRxiv - Bioengineering 2021Quote: ... All transformants were checked for the petite phenotype by growth on YEP-glycerol (1% w/v Bacto-yeast extract-BD Biosciences 212750, 2% w/v Bacto-peptone-BD Biosciences 211677 ...
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bioRxiv - Immunology 2021Quote: Cells resuspended in cold staining buffer (2% FCS in PBS) were blocked with mouse Fc Block (BD Biosciences, catalogue# 553141) for 10 minutes at 4°C and stained with a panel of antibodies (Supplementary Table 5) ...
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bioRxiv - Immunology 2021Quote: ... tibias and pelvis were harvested by crushing in PBS containing 2% of FBS and erythrocytes were lysed using a hypotonic buffer from BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... the invading cancer cells were fixed with 100% cold methanol and stained with 0.05 mg/mL 4,6-diamidino-2-phenylindole (DAPI; BD Biosciences, 564907). The invading cells in each chamber were counted under a fluorescence microscope ...
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bioRxiv - Cancer Biology 2020Quote: ... single cell suspensions were made (1.5 x 104 cells per ml) in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 150μl of this mix was plated per well in a 96 well ImageLock plate (Essen Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and the concentration of IL-2 in culture supernatants was determined by ELISA according to the manufacturer’s instructions (BD Biosciences).
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bioRxiv - Genetics 2021Quote: ... in 200 μl of 2% FBS/PBS (v:v) and calculated using DIVA before sorting on a FACSAria fusion III cytometer (BD Biosciences). In order to maintain the ratios of MuSCs ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5mM EDTA) containing the indicated fluorophor-conjugated antibodies (CD138-APC [281-2] and B220-PE [RA3-6B2] purchased from BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... were routinely grown at 37 °C under anaerobic conditions in a Bactron III-2 anaerobic chamber (Shellab, USA) in BHIS medium: 3.7% weight vol−1 brain heart infusion broth (BD, USA) supplemented with 0.5% weight vol−1 yeast extract (BD ...
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bioRxiv - Immunology 2019Quote: ... and stained for membrane extracellular markers in Staining Buffer (PBS FBS 2% EDTA 5mM) and for intracellular markers using Cytofix/Cytoperm buffer set following manufacturer’s protocol (BD Biosciences). Acquisition of cytometric parameters was performed on an LSRII (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: One hundred microliters of whole blood from each of the cynomolgus macaques was stained with combinations of fluorescence-conjugated monoclonal antibodies: anti-CD3 (clone SP34-2, Alexa700; BD), anti-CD4 (clone L200 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by fixation in 2% PFA in PBS before subjecting them to flow cytometry using the LSR-II (BD Biosciences). Data was analyzed by FlowJo v10 and graphed by Prism 8 (GraphPad).
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bioRxiv - Immunology 2020Quote: ... T cells were 2-way sorted from PBMC pre-gated on CD4+ CD14− viable lymphocytes using a FACS Aria (BD) and the antibodies listed in supplementary material procured from Biolegend or BD ...
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bioRxiv - Immunology 2021Quote: ... and I53-50B components of the NP-scaffold (1.2 µg/ml of each peptide) in the presence of 1 µg/ml of anti-CD28 (clone CD28.2, BD Biosciences) and anti-CD49d (clone 9F10 ...
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bioRxiv - Microbiology 2021Quote: ... were routinely grown at 37 °C under anaerobic conditions in a Bactron III-2 anaerobic chamber (Shellab, USA) in BHIS medium: 3.7% weight vol−1 brain heart infusion broth (BD, USA) supplemented with 0.5% weight vol−1 yeast extract (BD ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed 2 times with flow buffer and incubated with BD Cytoperm™ Permeabilization Buffer Plus (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... or formalin solution (2% in 1x PBS) in the plantar surface of the right hind paw using a standard 28G insulin syringe (BD) and video recorded from below for either 15 or 60 minutes respectively ...
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bioRxiv - Microbiology 2022Quote: ... Live cells were measured every 2-3 days on the BD FACS Celesta Flow Cytometer (BD Biosciences, Franklin Lakes, NJ) using a known amount of spike-in AccuCount Blank particles (Spherotech ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells then were trypsinized and resuspended in PBS containing 2% FBS and dTomato-positive cells were sorted using a BD FACSAria III flow cytometer (BD Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
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bioRxiv - Pathology 2024Quote: ... Lentivirally transduced LX-2 cells were sub-cultured until confluent before being flow-sorted on the BD FACSAriaTM (BD Biosciences) to obtain cells with top 10 % highest eGFP expression ...
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bioRxiv - Cell Biology 2024Quote: Twenty µl of platelet post activation supernatants were incubated with 2.5 µl of phycoerythrin (PE)-conjugated mouse anti-human CD62P (clone AK-2, BD Biosciences), 2.5 µl of PE-conjugated CD61 (clone VI-PL2 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were blocked for 30 minutes in FACS buffer (2% FBS 1X PBS) containing Fc Block anti-CD16/32 (2.4G2, BD Biosciences) (1:100 dilution) ...
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bioRxiv - Immunology 2024Quote: ... and then stained for 30 min at 4°C in PBA buffer (PBS, 0.5% BSA, 2 mM EDTA) in the presence of Fc Block (1:50 dilution, BD-Biosciences), using the following antibody combination ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Cytokine staining was performed with 2 x 106 cells after 3.5h of in vitro stimulation in Opti-MEM media supplemented with Golgiplug (BD Biosciences), 10 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: The titles and abstracts of 2 341 articles retrieved from the preliminary search were screened and cross-checked by two independent reviewers (ZL and BD) and discrepancies were resolved through discussion with a third researcher (DM) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 × 106 cells were harvested and resuspended in 100 μl of ice-cold 20% matrigel (BD Bioscience, Franklin Lakes, NJ) in PBS (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Immune cells were resuspended in surface staining mix containing FACS buffer with 1/2 of final volume of Brilliant Stain Buffer (BD), 50 μg/ml anti-mouse CD16/CD32 (2.4G2 ...
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bioRxiv - Immunology 2023Quote: ... LLC or CULA cells (2 × 105) resuspended into 50 ul DMEM and enriched with 20% extracellular matrix (Matrigel, BD Biosciences), were intrapleurally injected to the left lung lobe of mice using a 29G needle (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were sorted in PBS + 2 % FBS using a BD FACSAriaTM II or BD FACSArisa Fusion (both from BD Biosciences) cell sorters ...
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bioRxiv - Cancer Biology 2023Quote: ... Polyclonal goat anti-mouse Ig conjugated to APC was used as a secondary antibody (2 μg/mL, BD Pharmigen #550826). For all antibodies ...
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bioRxiv - Microbiology 2023Quote: ... FITC-A between 2×104–106) or an LSR-Fortessa flow cytometer (BD Biosciences; for Fig 5A and Fig S5C) and data was plotted in FlowJo (ver ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H ...
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bioRxiv - Cancer Biology 2023Quote: Spleens and livers were harvested into ice cold FACS buffer (PBS supplemented with 2% FCS v/v) and then emulsified through a 70μM filter (BD Biosciences), centrifuged and washed in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... Staining was quenched by washing in FACS buffer (PBS with 2 mM EDTA and 2% FCS) after which cells were incubated with anti-CD16/32 (Fc block, clone 2.4G2, BD Biosciences) for 15 minutes at 4°C to prevent non-specific binding ...
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bioRxiv - Developmental Biology 2023Quote: ... one million cells were transferred in 100 μl of FACS buffer supplemented with 0.5μM Y27632 and then added 2 μl Fc Block (BD Pharmingen, 553141) per sample followed by 10 min incubation on ice ...
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bioRxiv - Microbiology 2023Quote: ... 25 μL of control serum and 25 µL of serum pooled from all mice within a treatment group were pipetted onto a 2-mm diameter plain paper disc (BD) and allowed to dry for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... For microscopy experiments SDC-lysine media was used (2% glucose, 6.75 g/l yeast nitrogen base without amino acids (291929, BD Difco), 1.92 g/l yeast synthetic drop-out media supplements without lysine (Y1896 ...
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bioRxiv - Immunology 2023Quote: Bone marrow was isolated from dissected femurs that were gently crushed in PBS with 2% (v/v) FBS and subsequently filtered over a 70μm cell strainer (BD Falcon). Red cell lysis was done by incubating samples with ACK lysis buffer (Quality Biological ...
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bioRxiv - Immunology 2023Quote: ... The protein binding capacity of the well surface was saturated with 300 μL blocking solution (PBS supplemented with 2% skim milk powder (BD) and 0.1% Tween 20 ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 2 × 106 vector control or shACSL4 cells were mixed 1:1 by volume with Matrigel (BD Biosciences). Each mouse was injected orthotopically into both the flanks ...
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bioRxiv - Immunology 2023Quote: ... stained cells were resuspended in PBS 2% FBS and DC1 were purified in a FACS Aria II Cell Sorter (BD).
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bioRxiv - Cell Biology 2023Quote: Cytokine released by the SARS-CoV-2-infected or ApoBD-treated MDMs were measured using human cytokine bead array as per manufacturer’s instruction (BD Biosciences). Briefly ...
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bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...