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Citations for Becton, Dickinson and Company :
2951 - 3000 of 3331 citations for 8 4 dimethylaminophenyl diazenyl N N dimethyl 10 phenylphenazin 10 ium 2 amine chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cells grown on 96 well plates were fixed and permeabilized for 20 mins at 4°C with Fixation/Permeabilization solution (BD Biosciences, cat. no. 554714). Cells were washed 3 times in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... The stained cell suspensions were fixed in 4% paraformaldehyde and resuspended in PBS (1% BSA) before flow cytometry analysis using a FACSymphony™ A3 cell analyzer (BD Biosciences, NJ, U.S.A.).
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed in 4% paraformaldehyde and permeabilized using a commercially available saponin-based permeabilization and blocking reagent (1x BD Perm/Wash buffer #554723). Cells were then stained using primary antibodies against Flag (Proteintech 20543-1-AP ...
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bioRxiv - Microbiology 2022Quote: ... oxysporum conidia were isolated after growth for 3-4 d in potato dextrose broth (PDB; Becton, Dickinson and Company, Le Pont de Claix, France) by filtering through two layers of Miracloth® (Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4°C with primary antibodies: ALIX (BD Biosciences, 611620, 1:1000), calnexin (Santa Cruz ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min (RT) and incubated overnight (4 °C) with mouse anti-5-LOX (1:1000; # 610695; Lot: 9067817; BD Biosciences, Franklin Lakes, NJ) and rabbit anti-perilipin-2 (1:100 ...
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bioRxiv - Bioengineering 2022Quote: ... Brooks Lifesciences) were coated with 200 μL of 2 mg/mL type 1 rat tail collagen (354249, Corning/BD Biosciences) diluted in DMEM equilibrated in 1/10 v/v of 7.5% w/v sodium bicarbonate solution (#25080-060 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were fixed in 2% PFA and subsequently acquired using a BD LSRFortessa flow cytometer (BD Biosciences, San Jose, CA). Flow data were analyzed using FlowJo v10 software (Tree Star ...
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bioRxiv - Immunology 2021Quote: ... cells were either resuspended in 400μl of PBS 2%FCS 2mM EDTA and analyzed on Fortessa-X20/Symphony cytometers (BD Biosciences) or in RPMI media and used for cell sorting on a FACSAria II (BD ...
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bioRxiv - Immunology 2021Quote: ... Vα7.2+ cells were pre-incubated for 1 h and 30 min with LukED at 0.312 μg/mL before adding to the bacteria-pulsed THP-1 and co-culture at 2:1 ratio for 24 h in the presence of 1.25 μg/mL anti-CD28 mAb (L293, BD Biosciences). Monensin and brefeldin A (both from BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... whole blood was stained within 2 hours of blood draw for the surface markers CD45 (PerCP; DO58-1283; BD Biosciences), CD3 (FITC ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed in 2% paraformaldehyde and were examined in a Canto II flow cytometer (BD Bioscience, San Jose, CA), following a previously reported protocol [49] ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mL of cells at 100,000 cells/mL in PBS-BSA was loaded in a 3 mL syringe (BD, #309657). A 125 μm Drop-seq microfluidic device was used for droplet generation (31) ...
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bioRxiv - Immunology 2021Quote: ... at 100ng/mL and IL-2 (100IU/mL) or expanded by plate-bound anti-CD3 mAb at 2μg/mL (BD Pharmingen) and soluble anti-CD28 mAb at 1μg/mL (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... and microglia (CD45med CD11b+were sorted into collection tubes coated with FCS and filled with complete RPMI 1640 medium (1% penicillin, streptomycin, 0.1% 2-ME) with 20% FCS using a FACSAria device (BD Biosciences). Purity of sorted populations was routinely above 95% ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured in T cell media supplemented with soluble anti-mouse CD28 (2 μg/ml, Clone: 37.51, BD Pharmingen) and 30 U/ml rhIL-2 (Roche ...
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bioRxiv - Bioengineering 2021Quote: ... at 2×106 cells/ml in wells coated with anti-mouse CD3e (1 μg/ml, Clone: 145-2C11, BD Pharmingen).
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bioRxiv - Bioengineering 2020Quote: ... The concentrations of cytokines IL-2 and IFN-γ were quantified using the corresponding ELISA kits (BD, 555190 and 555142).
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies diluted in PBS containing 2% NHS overnight (1:500 rabbit anti-Iba1, Abcam, ab178846; 1:200 mouse anti-Map2, BD Pharmagen ...
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bioRxiv - Immunology 2020Quote: ... 2 x 106 cells were cultured in 200μL final volume in 5mL polypropylene tubes (BD Biosciences, San Diego, CA, U.S.A) in the presence of anti-CD28 (1μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... xenograft models were generated by injecting SKOV3 cells [2 × 106 cells mixed 1:1 (v/v) with Matrigel (BD Biosciences)] subcutaneously ...
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bioRxiv - Systems Biology 2021Quote: ... The concentration of the cell suspension was adjusted to 2×106 cells ml-1 and subsequently aspirated into a 3 ml luer-lock syringe (BD).
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bioRxiv - Microbiology 2020Quote: 100 μl of washed and number-adjusted yeast suspension were injected into each caterpillar’s distal right proleg with a 30G1/2” needle (BD Microlance) and a 1 ml NORM-JECT syringe ...
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bioRxiv - Genomics 2020Quote: Primary human bronchial epithelial basal cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then washed with FC buffer (PBS supplemented with 2% HI-FBS and 0.05% NaN3) and resuspended in Fixation Buffer (BD Pharmingen)for 5 min at 4C° in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... and Western blots (WBs): monoclonal Abs (mAbs) anti-flotillin-2 (610383; WB: 1/1000) and anti-GM130 (610823; IF: 1/500) from BD Biosciences ...
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bioRxiv - Developmental Biology 2022Quote: ... E.coli strain Rosetta™ 2(DE3)pLysS bearing pET21d-FGF10 was grown in 2x YT medium (BD Biosciences, Cat#244020) with Carbenicillin (50µg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Collagenase activity was quenched by dilution with FACS buffer (PBS, 0.5% FBS, 2 mM EDTA) and digestion products were filtered through 70 µm nylon filters (BD Biosciences). Cells were again washed with FACS buffer and immediately processed for immunostaining first by blocking Fc receptor binding with rat anti-mouse CD16/CD32 (eBioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... Flow cytometry profiles of the HSPC compartment (Extended Data Fig.2, Fig.9) were analysed using FlowJo software (version 10.1, BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Pellets were resuspended in 2 ml of FACS buffer and fixed by adding 3.5 ml of fixation/permeabilization buffer (BD Biosciences) and rocking overnight at 4C ...
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bioRxiv - Pathology 2021Quote: ... Whole blood samples were then lysed and fixed in 2 ml of FACS Lysing Solution (BD Biosciences, San Jose, CA) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were first blocked for non-specific antigen binding (incubating for 20 min at RT with 2 µg/ml CD16/CD32 Rat anti-Mouse, unlabeled, Clone: 2.4G2, BD, 553142) then antibodies were added without washing off the blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained with antibody (Supplementary Table. 2) and LSK cells were isolated through cell sorting using FACSAria (BD). Sorted LSK cells were cultured in StemSpan SFEM serum free media (stemcell technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Pre-coated coverslips were printed by stamping (0.2 mm spot diameter and 0.5 mm spot interspace) with a cell-attachment mixture of rat tail collagen (BD Biosciences), poly-D-lysine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... After staining for 45 min at 4°C cells were washed and taken up in 200 µl PBS+2%FBS and 1/1000 7AAD before being run on a LSR Fortessa (BD). Lineage out-put was analysed in FlowJo ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of cell lysate premixes were pipetted to another flat-bottomed 96-well reaction plate containing 100 μl reaction buffer with 270 nM type 2 RNase H-specific substrate (BD) using a multi-channel pipette ...
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bioRxiv - Biochemistry 2022Quote: ... washed and resuspended in FACS buffer (2% FBS in 1X PBS) and filtered through filter-topped flow tubes (BD falcon) using a luer-lock syringe at 2×106 cells/ml ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cells were resuspended in PBS supplemented with 2% FBS and then analyzed with BD FACSCalibur™ (BD, Bedford, MA, USA). Raw data were analyzed by FlowJo software.
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bioRxiv - Biochemistry 2019Quote: ... shRNA expression was induced by doxycycline (2 μg/ml) and sorted using a BD FACSAria™ III (BD Biosciences-US) cell sorter to select for the high TurboRFP expressing cells ...
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bioRxiv - Genomics 2019Quote: ... 2 mL of cells at 200,000 cells/mL in PBS-BSA was loaded in a 3 mL syringe (BD, #309657). A custom-built 90 μm Drop-seq microfluidic device (CAD file supplied separately ...
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bioRxiv - Bioengineering 2019Quote: Blood was gently drawn from healthy volunteers who had not been subjected to any anticoagulant treatments for at least 2 weeks by standard venepuncture into sodium citrate vacutainers (2.7 mL, 3.2 % v/v sodium citrate; BD, NJ, USA). The informed consent was provided by each volunteer ...
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bioRxiv - Immunology 2019Quote: ... and activated with 2 μg/mL anti-mouse RP105 clone RP/14 (produced in house or BD Pharmingen Cat.# 562191).
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bioRxiv - Microbiology 2020Quote: ... cells were resuspended and 2-43 binding to cell-surface S trimer was quantified on LSRII flow cytometer (BD Biosciences). The mean fluorescence intensity of APC in GFP-positive cells was analyzed using FlowJo and the relative binding of 2-43 to S trimer in the presence of competitors was calculated as the percentage of the mean fluorescence intensity compared to that of the competitor-free controls.
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bioRxiv - Immunology 2020Quote: ... Then cells were fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). For cell sorting ...
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bioRxiv - Systems Biology 2019Quote: ... washed in PBS and 250k cells were stained with PE-LDLR at 2 µg/ml (1:100, BD Bioscience 565653) for 20 minutes at 4°C and Zoombie NIR (BioLegend 423105 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer (PBS with 2% Heat-inactivated FCS) and fixed in 1x BD cellFIX (BD Biosciences). Samples were acquired on the LSRFortessa (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were washed with 2 ml wash buffer and taken up in 0.5 ml wash buffer afterwards for FACS analysis (BD Fortessa).
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bioRxiv - Immunology 2020Quote: ... the cells washed and stained with CD45.2-FITC (to identify leukocytes) and cell subset specific fluorochrome-conjugated mAb used in this study were obtained from BD Biosciences and Biolegend ...
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bioRxiv - Genetics 2019Quote: ... in PBS with 1 μg/ml bovine serum albumin (BSA and 2 mM Tris-HCl. Cells were analyzed on a BD LSRII flow cytometer (BD Biosciences ...