Labshake search
Citations for Becton, Dickinson and Company :
2851 - 2900 of 3331 citations for 8 4 dimethylaminophenyl diazenyl N N dimethyl 10 phenylphenazin 10 ium 2 amine chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then spun down at 500 × g for 5 min at 4°C and resuspended with the following antibody-conjugated fluorophores for 20 minutes at room temperature: anti-CD3-BV421 (BD, 1F4, 1:100), anti-CD4-PE-Cy7 (BD ...
-
bioRxiv - Cell Biology 2019Quote: ... stably transduced mApple and eGFP EpH4 cells were sorted using a BD 4 laser FACSAria III cell sorter (BD Biosciences, San Jose, CA), treated for 24 hours with 1 μM Cisplatin or PBS vehicle ...
-
bioRxiv - Systems Biology 2021Quote: ... HeLa and RAW 264.7 cells were washed by chilled PBS and sorted on the nanoPOTS chips (4 × 12, 1.2 mm diameter per well) using the Influx II cell sorter (BD Biosciences, San Jose, CA) as described previously 14 ...
-
bioRxiv - Immunology 2020Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... were plated around the rim of the well of a 96-well plate in a 4:3 mixture of Matrigel and MammoCult medium (BD Bioscience CB-40324). The cell mixture was then incubated at 37°C with 5% CO2 for 15 min to solidify the gel ...
-
bioRxiv - Immunology 2022Quote: ... mice were euthanized and tumors were quickly harvested and fixed for 14-16 hr at 4&C in BD Cytofix/Cytoperm (BD Bioscience, Cat #: 554722) diluted 1:4 in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... 98 mL of fresh MRS medium is then inoculated with 2 mL of the overnight culture and incubated at 37°C without shaking to an OD of 0.5-0.6 (ca. 4-5 hours) in a GasPak anaerobic jar (BD GasPak™ EZ container systems). Cells are harvested a first time by centrifugation at 4,000 g for 5 min ...
-
bioRxiv - Systems Biology 2022Quote: Single cell suspensions were plated around the rim of the well in the 3:4 mixture of Mammocult and Matrigel (BD Bioscience CB-40324). We seed 5’000 cells/wells for mini-rings in white 96-well plates (Corning #3603 ...
-
bioRxiv - Microbiology 2022Quote: ... Afterwards the samples were fixated and permeabilized for 20 min and 60 min at 4 °C in the dark using BD Cytofix/Cytoperm™ solution and BD Perm/Wash™ solution (BD Biosciences), respectively ...
-
bioRxiv - Microbiology 2022Quote: ... the individual co-cultures were 10-fold serially diluted with 1X anaerobic phosphate buffer saline (PBS) and plated on Xylose Lysine Tergitol 4 (XLT4) agar (BD Difco, Houston, TX). The plates were incubated aerobically at 37°C for 24 hours followed by plating on XLT4 agar and colony forming units (CFU ...
-
bioRxiv - Neuroscience 2024Quote: ... and samples were incubated in the 9-well plates with 100 μL per plate overnight at 4°C: CtBP2 mouse IgG1 (BD Biosciences 612044; RRID:AB_399431), GluA2 mouse IgG2a (Millipore MAB397 ...
-
bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
-
bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
-
bioRxiv - Immunology 2023Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... overnight and then stimulated with 1 nM N4 in the presence of vehicle or Compound 182 (1 μM) for 4 h at 37°C in complete T cell media supplemented with BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were then stained with fluorophore-conjugated antibodies to cell surface CD8 and intracellular IFNγ ...
-
bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667, 1:100 in 1X BD Perm/wash solution) or anti-BST2 (Proteintech #13560-1-AP ...
-
bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was washed with 3-4 ml washing buffer which was fresh PBSB 1% and then was filtered through a 70-µm cell strainer (BD Bioscience, NY, USA). Then using a long tube ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
-
bioRxiv - Genomics 2023Quote: ... and half of the remaining chromatin was rotated overnight at 4°C with 4.5 µg of anti-p53 (BD Biosciences #554293, Lot #9046678). The other half of the remaining chromatin was also rotated overnight as a no-antibody control ...
-
bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
-
bioRxiv - Immunology 2021Quote: ... 558121), CD11c (clone 3.9, 748288), CD3 (clone SP34-2, 557757) and ITGB7 (clone FIB504, 555945) were purchased from BD Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: Primary human bronchial epithelial cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
-
bioRxiv - Bioengineering 2022Quote: Cells were fixed in 2% PFA for 15 minutes and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... Fixed cells were incubated with either conformation specific BAK antibody (Clone# G317-2, 1:100) (Cat# 556382, BD Pharmingen) followed by phycoerythrin (PE)-conjugated anti-mouse antibody (1:200 ...
-
bioRxiv - Immunology 2020Quote: ... cells were fixed/permeabilized using BD FACS lysing solution and permeabilizing solution 2 according to manufacturer’s instructions (BD Biosciences) followed by intracellular staining with Ki-67-PE and t-bet-BV711 (Biolegend) ...
-
bioRxiv - Microbiology 2019Quote: ... and 10 healthy controls (sampled once each) were quantified by two-step sandwich ELISA following the manufacturer’s instructions (Peprotech Human BD-2 and BD-3 ...
-
bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences, Custom Bulk 624040, PerCP-Cy5.5; BD Biosciences ...
-
bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
-
bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
-
bioRxiv - Microbiology 2021Quote: ... Intracellular staining was performed using rat anti-mouse IFN-γ-PECy7 (clone XMG1-2, 1:300, BD cat#557649), rat anti-mouse IL-2-PE (clone JES6-5H4 ...
-
bioRxiv - Cancer Biology 2020Quote: ... CWR22Rv1 xenografts were established in the flanks of mice by injecting ~2 million cells in 50% matrigel (BD Biosciences). When tumors reached approximately 200mm3 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in blocking buffer (PBS with 2% FBS) containing an anti-BrdU monoclonal antibody (BD Biosciences) (1:100 dilution ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were sorted at a concentration of 2 million cells per mL using FACS- Aria-III (BD Biosciences). Forward and side scatter parameters were adjusted so as to eliminate cell clumps and debris ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 2% fetal-bovine serum in PBS and analyzed using the LSRFortessa flow cytometer (BD Biosciences). A total of 20,000 events were collected for each sample ...
-
bioRxiv - Immunology 2022Quote: ... All experimental steps were performed in a 50/50 mixture of PBS + 2% FBS and Brilliant Buffer (BD Bioscience). Antigen probes for Spike ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2×105 cells were washed with PBS1X and resuspended in 100 µL of Annexin-V binding buffer (BD Biosciences). 1 µL of Annexin-V-APC (Cat# 550474) ...
-
bioRxiv - Immunology 2020Quote: ... T cells and DC were stained with anti-syndecan-1 (rat IgG2Aκ monoclonal antibody, clone 281-2, BD Biosciences) or isotype control (rat IgG2Aκ ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2% FBS) and analyzed for green and red fluorescence by flow cytometry using a BD FACSymphony instrument (BD-Biosciences).
-
bioRxiv - Cancer Biology 2021Quote: ... Spheroids were harvested the next day and transferred in a collagen type I suspension (2 mg/ml) (BD/corning) (354249 ...
-
bioRxiv - Immunology 2022Quote: ... SiglecF+) as described in Figure S7: anti-CD45-Brilliant Violet 605 (2 µg/ml, clone 30-F11; BD Bioscience), anti-CD11b APC-Cy7 (1 µg/ml ...
-
bioRxiv - Genomics 2020Quote: ... Stained cells were resuspended in FACS buffer (PBSS + 2% FBS) and analyzed by FACS using BD LSRFortessa (BD Bioscience).
-
bioRxiv - Immunology 2021Quote: ... Dishes were kept undisturbed for 2 min and the clear supernatant was pipetted out slowly into cell strainer (BD). The filtrate was collected in sterile tubes and the cells were pelleted by centrifugation at 4°C for 10min at 250×g in a centrifuge (Thermo Scientific) ...
-
bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™ ...
-
bioRxiv - Developmental Biology 2019Quote: ... were cultured in DMEM/F12 (PAA Laboratories Inc.) supplemented with 2% Ultroser SF (i.e. steroid free; BioSepra) and 1% ITS+ (BD) at 37°C in humidified 5% CO2 and 95% air for 64 hours ...
-
bioRxiv - Immunology 2021Quote: T-cell responses were characterized by measurement of the frequency of PBMC expressing IL-2 (PerCP5.5, MQ1-17H12, BD), IL-17a (Alexa700 ...
-
bioRxiv - Immunology 2020Quote: PBMCs (1−2×106) were stimulated with epitope-specific peptides (5 μM) and anti-CD28 mAb (0.5 μg/mL, BD) and expanded for 14 days in complete RPMI culture medium containing rIL2 (20 IU/mL ...