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Citations for Becton, Dickinson and Company :
2301 - 2350 of 2978 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... All experimental steps were performed in a 50/50 mixture of PBS + 2% FBS and Brilliant Buffer (BD Bioscience). Antigen probes were prepared individually and mixed together after multimerization with 5μM free D-biotin (Avidity LLC ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed in PBS+2% FCS and permeabilised for 10 minutes in 70ul of Cytofix/Cytoperm (BD Biosciences). After washing in 2mL of PermWash ...
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bioRxiv - Neuroscience 2023Quote: ... Digested suspensions were subsequently triturated by sterilized syringes with 20G 1/2 needle (BD Biosciences, Franklin Lakes, NJ, USA) and washed with DMEM to harvest mononuclear cells ...
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bioRxiv - Immunology 2023Quote: ... 2×106 cells/well (6-well plate) were cultured with 1μg/mL of anti-CD3 mAb (553057, BD Biosciences) plus 10 ng/mL of mouse IL-2 (Z02764 ...
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bioRxiv - Immunology 2023Quote: ... 2) As the MojoSort antibodies are biotinylated we replaced the Qa2-bio antibody with Qa2-BV421 (BD, Cat. 743309). Using qPCR and FACS we ensured that the positively selected (lineage positive ...
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bioRxiv - Immunology 2023Quote: ... 5 x104 – 2 x105 leukocytes were examined per sample with a FACSCanto II (BD Biosciences, Franklin Lakes, NJ, USA), according to established multi-color protocols (Cossarizza et al. ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in sort buffer (2% FBS in PBS + 2mM EDTA) and analysed on the Fortessa X20 (BD) flow cytometer ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in surface staining mix containing FACS buffer with 1/2 of final volume of Brilliant Stain Buffer (BD), 50 μg/ml anti-mouse CD16/CD32 (2.4G2 ...
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bioRxiv - Immunology 2024Quote: ... and then for a further 2 hours with the addition of 1 mg/ml Brefeldin and Monensin (BD Biosciences) in complete medium ...
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bioRxiv - Immunology 2024Quote: ... Then stained cells were washed with PBS containing 2% FBS and detected by LSR II flow cytometer (BD Biosciences). The data was analyzed by FlowJo v10 software (Ashland ...
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bioRxiv - Immunology 2024Quote: Depletion assessment mix of antibodies for flow cytometry staining: 2 µL of anti-CD4-FITC (BD Biosiences, Ref. 345768), 1 µL of anti-CD33-PE (Invitrogen eBioscience ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... at 37°C, 180 rpm, in SD medium (2% glucose, 0.67% yeast nitrogen base with amino acids (YNB) (BD), pH 5.4) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mM EDTA in 1X PBS) and filtered through a 5 ml cell-strainer capped FACS tube (BD Falcon). A BD Biosciences FACSAria Fusion was used to sort tdTomato negative or tdTomato positive cells for gene expression analysis ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended in 50 μL 3% FBS/ 1X PBS buffer with 2 μL FC block (BD cat# 553141) and incubated on ice for 20 min ...
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bioRxiv - Immunology 2024Quote: ... T cells were activated for 2 days in plates coated with anti-CD3 (OKT3) and anti-CD28 (CD28.2) antibodies (BD) and transduced with CAR-encoding lentiviral particles at a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2024Quote: ... 2 days after transduction GFP+ or mcherry+ cells were purified by flow cytometric cell sorting (BD FACS AriaTM Fusion) prior to adoptive transfer into recipient mice ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were incubated with either conformation-specific BAK antibody (Clone#G317-2, 1:100, Cat# 556382, BD Pharmingen), BAX antibody (Clone#3 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... and the live pelleted cells were resuspended in PBS and analyzed with a BD Accuri C6 flow cytometer (BD Biosciences, San Diego, CA) equipped with an FL1 laser (515–545 nm) ...
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bioRxiv - Cell Biology 2020Quote: ... flow cytometry was performed using a BD LSRFortessa X-20 or BD LSR-II analytical flow cytometer (BD Biosciences, San Jose, CA, USA) and FCS files were analysed using FlowJo version 10 (Tree Star Ashland ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 0.1% BSA and were resuspended in 0.1% BSA/HBSS and analyzed with Accuri C6 benchtop flow cytometer (BD Biosciences, San Jose, CA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... then were seeded into a 96-well plate at a density of 5×103cells/well coated with 10mg/ml Matrigel matrix without growth factor (Cat No: 3432-010-01, BD Biosciences, CA, USA) for 6 hour incubation at 37□ ...
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bioRxiv - Molecular Biology 2020Quote: ... Edited and non-edited cells were analyzed by flow cytometry using the following antibodies: hCD45 V450 (HI30; BD Biosciences, San Jose, CA, USA), CD34 APC (561 ...
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bioRxiv - Neuroscience 2020Quote: ... and then were stamped with a 1:1 mixture of rattail collagen (final concentration 1.0 mg/mL, BD Biosciences, San Jose, CA, USA) and poly-D-lysine (final concentration 0.25 mg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then washed in 1X AnnexinV buffer and stained with AnnexinV and 7-amino-actinomycin D (7-AAD) according to the manufacturer’s protocol (BD Pharmingen, San Diego, CA). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... the co-cultured cells were trypsinized and analyzed by Flow cytometry in FITC and Rh channel using a BD FACS Aria flow cytometer (BD Biosciences, CA, USA) for quantification ...
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bioRxiv - Bioengineering 2020Quote: ... At selected timepoints following FACS the cells were analyzed for tdTomato fluorescence using a FACSCanto flow cytometer (BD Biosciences, San Jose, CA, USA). Either 14- or 21-days post the initial sort ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell surface phenotypes were determined by direct immunofluorescence staining with conjugated mAbs and analyzed using FACS Calibur (BD Biosciences, San Jose, CA). Digested cells were stained with αCD3 ...
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bioRxiv - Plant Biology 2020Quote: The protoplast-filled digestion solution was first filtered through a Falcon 100 μm nylon cell strainer (352360; BD Biosciences, San Jose, CA, USA) in a sterile petri dish and then through a Falcon 40 μm nylon cell strainer (352340 ...
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bioRxiv - Microbiology 2020Quote: ... Assay plates were incubated overnight at 37ºC in a 5% CO2 incubator and developed using BD ELISPOT AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISPOT reader and Immunospot software (Cellular Technology ...
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bioRxiv - Genomics 2021Quote: ... and stained for anti-hCD45 (APC-R700, clone HI30), anti-hCD235a (PE-Cy5.5, clone GA-R2), anti-hCD3 (PE-Cy7, clone SK7) (all from BD Biosciences, San Jose, CA) and anti-hHLA-DR (BV785 ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 2M HCl for 30 minutes and stained with mouse monoclonal anti-BrdU antibody (1:50) (BD Biosciences, San Jose, CA, USA) and Alexa fluor-488 conjugated secondary antibody (1:200 ...
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bioRxiv - Neuroscience 2020Quote: Western analyses were run as described[33] with the following changes: Purified mouse anti-ASyn antibody clone 42 (BD Biosciences, San Jose, CA) was diluted at 1/1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 Capan-1 cells and 2.5×104 Hs766T cells were plated in coated BD BioCoat growth factor-reduced matrigel chambers (BD Biosciences, San Jose, CA) for 24 hours ...
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bioRxiv - Cell Biology 2021Quote: ... were detected by high-resolution multi-parametric flow cytometry analysis and results were analyzed using Cyflogic V1.2.1 (CyFlo Ltd., Turku, Finland), FlowJo (FlowJo LLC., Ashland, Oregon) and BD CellQuest Pro (BD Biosciences., San Jose, CA, USA). Calibration of flow cytometer was performed with the three-millimeter rainbow calibration particles (Spherotech ...
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bioRxiv - Cancer Biology 2022Quote: ... The apoptotic rates were analyzed by the Annexin V-FITC and propidium iodide (PI) double detection kit (BD Biosciences, San Jose, CA, USA), and the rinsed cells were resuspended in binding buffer containing Annexin V-FITC and PI and then incubated at RT for 15 min in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... after 24 hours cells were collected with PBS supplemented with 5mM EDTA and analyzed at medium flow rate in flow cytometer FACS Aria III (BDBiosciences, San Jose, CA) and FlowJo software was used for analysis ...
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bioRxiv - Microbiology 2021Quote: ... Total gB expression was determined by using the same staining protocol except cells were permeabilized using Cytofix/Cytoperm (BD Biosciences, San Jose, CA) before adding the primary antibody and during the staining procedure ...
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bioRxiv - Immunology 2020Quote: ... and the purity of monocytes was evaluated by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: We permeabilized formalin-fixed glomerular and tubulointerstitial single cells with 0.25% Tween-20 in PEB buffer at room temperature for 30 minutes and blocked cells with Fc Block (clone 2.4G2, BD Biosciences, San Jose, CA) diluted in 0.25% Tween-20 on ice for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... At the end of the incubation plates were washed 5x and developed with BD OptEIA TMB substrate reagent set (BD, San Jose, CA) for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... obtained from bone marrow of tumor-free GFP-expressing mice were mixed with B16-F1 or B16-F10 cells in Matrigel (BD Biosciences, CA, USA) and subdermally implanted into the flanks of C57BL/6 mice ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was seeded into the upper chamber of SPLInsert with Polyethylene terephthalate (PET) membrane (pore size: 8.0 μm) (SPL Lifesciences, Korea) pre-coated with matrigel (BD Biosciences, San Jose, CA). The bottom well of 24-well plate was filled with cell medium containing 10% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... or LB media (10 g/liter Bacto tryptone, 5 g/liter Bacto yeast extract, 10 g/liter sodium chloride; BD, San Jose, CA) at 30°C ...
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bioRxiv - Cancer Biology 2020Quote: ... The eGFP-ffluc-transduced PDX6 cells were selectively isolated based on GFP expression via FACSAria cell sorter (BD Biosciences, San Jose, CA, USA). PDX6 cells were injected into the right ovarian bursa of nude mice with Matrigel (354248 ...
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bioRxiv - Immunology 2020Quote: ... The cells were subsequently washed with FACS buffer (1% BSA and 0.1% sodium azide in phosphate-buffered saline) and stained with anti-HLA-A2 specific monoclonal antibody (clone BB7.2) (BD-Pharmingen, San Diego, CA) at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... cerevisiae cells were grown at 25°C in YPD liquid medium containing 1% (w/v) Bacto yeast extract (BD Biosciences, San Jose, CA), 2% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Off-line samples were taken from the reactors each 5-10 h and the analysis of the GFP expression levels was performed with a BD Accuri C6 (BD Biosciences, CA, USA) based on FL1-A channel (excitation 488 nm ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cultures were routinely grown in lysogeny broth (LB) broth at 37°C with aeration or on LB solidified with 1.5% agar (BD Biosciences, San Jose, CA). When stated ...
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bioRxiv - Neuroscience 2021Quote: ... the BECs were seeded in 96-well plates coated with collagen type I (5 µg/mL, #354231, BD Bioscience, San Jose, CA, USA) at a density of 104 cells per well ...