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Citations for Becton, Dickinson and Company :
2401 - 2450 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cellular uptake was detected in the mCherry channel after acquisition of 10,000 single cells per sample using a flow-activated cell sorter (FACS; FACS Aria III, BD Biosciences). Analyses were conducted by FCS Express (De Novo software).
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bioRxiv - Cell Biology 2020Quote: ... cells were re-suspended in growth media and sorted using a FACSAria IIu cell sorter (BD biosciences, San Jose, USA). The machine was previously calibrated with non-transfected cells of each cell type and baseline for fluorescence was set ...
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bioRxiv - Immunology 2021Quote: ... Single cells were isolated with the BD Rhapsody Express Single-Cell Analysis System according to the manufacturer’s recommendations (BD Biosciences). cDNA libraries were prepared using the BD Rhapsody Whole Transcriptome Analysis Amplification Kit (633801 BD Biosciences ...
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bioRxiv - Immunology 2021Quote: Splenocytes and MLN leukocytes were sorted for naïve CD4+ cells (live CD4+ CD25- CD44lo CD62L2+) using a BD FACSAria cell sorter (BD Biosciences). 1×106 cells were plated per well of a 48 well plate pre-coated with 2 µg/ml anti-CD3 and anti-CD28 antibodies for 4 hours at 37°C 5% CO2 ...
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bioRxiv - Genetics 2021Quote: ... 250 µl of cells were transferred into 96-well plates and 30,000 cells were measured in a Fortessa X20 Flow cytometer (BD Biosciences), using the HTS plate mode on the DIVA software and a 488 nm excitation laser to capture the SytoxGreen DNA staining.
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bioRxiv - Developmental Biology 2020Quote: ... Labeled cells were washed and resuspended in Cell Staining Buffer before being analyzed on a LSRII flow cytometer (BD Biosciences) and using software packages CellQuest and FlowJo v10.
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bioRxiv - Genomics 2020Quote: ... Cell aggregates were removed by passing suspensions though a 50 μm cell strainer (FALCON) into a polypropylene FACS tube (BD). To avoid Hoechst toxicity ...
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bioRxiv - Microbiology 2022Quote: ... Cell quantities were quantified with flow cytometry using the BD Cell Viability Kit with BD Liquid Counting Beads (BD Biosciences). Cultures were then centrifuged at 3,000 g for 15 minutes and washed with sterile PBS three times before dilution into sterile water to a final concentration of 9x105 cfu/mL.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were sorted using a 100 μm nozzle (at 20PSI) on a BD FACSARIA III cell sorter (BD Biosciences, UK) with cooling of the sample and collection chambers enabled ...
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bioRxiv - Developmental Biology 2022Quote: ... flow cytometry data acquisition and cell sorting were carried out using FACSAria III cell sorter (BD Biosciences, San Jose/CA). Data were analyzed using FlowJo software version X (FlowJo ...
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bioRxiv - Genomics 2020Quote: ... to purify Lin-Sca1+cKit+ Flt3-cells (HSPCs) by multi-parameter fluorescence-activated cell sorting (FACS) on a FACSAriaII (SpecialOrderResearch Products; BDBiosciences). Flow cytometry analyses were performed using a BD-LSRII cytometer and analysed using BD-DIVA Version 6.1.2 software (Special Order Research Products ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells from spleen and LN were obtained by mashing through a 100 μm nylon cell strainer (BD Falcon). Red blood cells were lysed with a hypotonic Ammonium-Chloride-Potassium (ACK ...
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bioRxiv - Immunology 2021Quote: ... Viable cells were distinguished from dead cells by using Fixable Viability Dye eFluor780 (eBioscience) and stained cells were acquired at a Canto II Flow Cytometer (BD).
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bioRxiv - Immunology 2021Quote: Both Lin-CD25+CD44- DN3 cells and Lin-CD25-CD44- DN4 cells were sorted from the thymi of WT mice by FACSAria flow cytometry (BD). Anti-Zfp335 antibody (Novus ...
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bioRxiv - Cell Biology 2019Quote: Flow cytometry of anti-p-ERK-stained CRC cells resuspended in PBS/1%BSA cells was done using an Accuri cytometer (BD). Cells were gated for populations displaying different tdTomato fluorescence values (negative ...
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bioRxiv - Immunology 2019Quote: Tumor cells or T cells suspended in FACS staining buffer were stained with fluorochrome-conjugated antibodies against CD3e (SK7, BD) and murine TCRβ (12-5961-82 ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescence of 10,000 cells per sample was determined by flow cytometry using the BD LSRFortessa™ Cell Analyzer (BD Biosciences) with the HTS auto-sampler device ...
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bioRxiv - Systems Biology 2019Quote: Total T cells were isolated from peripheral blood mononuclear cells from 3 human donors via negative selection (Stem Cell Technologie cat. no. 19051) and plated in anti-CD3 (BD Pharmingen clone UCHT1 ...
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bioRxiv - Genetics 2020Quote: ... One ml of the epididymal cell-containing medium was carefully filtered through a 100 μm cell strainer (BD Biosciences, Inc.) into a 1.5 ml microfuge tube prior to counting using a Hemocytometer chamber ...
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bioRxiv - Neuroscience 2020Quote: ... The genetically labelled live cells were separated based on green or red fluorescence intensity using fluorescence-activated cell sorting (FACS Aria III, BD). FACS-purified cells were collected directly in lysis buffer of the RNeasy® Micro Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 50 ng p24 equivalent volumes were added to TZM-bl cells and cells were fixed/permeabilized with Cytofix/Cytoperm (BD) at 48 hours post-infection ...
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bioRxiv - Synthetic Biology 2021Quote: Cell lines were bulk sorted for high expression using the UCSF Laboratory for Cell Analysis Core Facility FACSAriaII (BD Biosciences). Cells were assessed for mCitrine (488nm excitation ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in PBS/0.5% BSA buffer (5 x 106 cells/ml) and immediately sorted using a FACS Aria cell sorter (BD Biosciences). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... cells were analysed with an LSR II instrument and isolated with an Aria I fluorescence-activated cell sorter (BD Biosciences).
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bioRxiv - Neuroscience 2020Quote: ... were acquired and generated by the Tau Consortium Stem Cell Group as previously described.30 Cells were maintained on Matrigel (BD) in mTeSR1 medium (Stem Cell Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... LLC-CC and LLC-PON1 cell suspensions (1.2×107 cells) in 200 μL of culture medium/growth factor-reduced Matrigel (BD Biosciences) in a 1:1 ratio were subcutaneously injected into the right flank of each mouse ...
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bioRxiv - Cell Biology 2021Quote: ... The cell suspension was passed through a 100 µm and a 40 µm cell strainer (BD Biosciences, San Jose, CA) and pelleted by centrifugation at 200G at 4°C for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were washed 1x in ice-cold PBS (0.8mM CaCl2) and acquired on a LSRFortessa Cell Analyzer (BD Biosciences). The experiment was performed in two independent replicates and analysed by FlowJo v10.6.1 (FlowJo LLC).
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bioRxiv - Pathology 2021Quote: Memory B cells were isolated from peripheral blood mononuclear cells (PBMCs) through magnetic cell sorting with 0.5 μg/ml anti-CD19-PECy7 antibodies (BD, 341113) and mouse anti-PE microbeads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: ... HeLa and HAP1 cell lines were transfected with the virus particle preparations in presence of 10 µg/ml polybrene and after three sub-culturings the resulting transgenic cell lines were sorted on a FACSAria III cell sorter (BD).
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bioRxiv - Microbiology 2020Quote: ... Culture supernatants were screened for specificity by ELISA and positive cultures were single cell sorted using a Beckman Coulter Legacy MoFlo MLS High Speed Cell Sorter (BD). Monoclonal cultures were expanded in DMEM (Sigma ...
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bioRxiv - Physiology 2021Quote: ... into two cell populations: CD19+CXCR3+ITGB1+ and CD19+CXCR3-ITGB1-using the BD Aria I cell sorter (BD Biosciences). The cells were centrifuged at 300g ...
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bioRxiv - Cell Biology 2021Quote: ... - the average number of cell divisions that a cell in the original population has undergone was calculated using FlowJo software (BD).
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bioRxiv - Cell Biology 2020Quote: Transfected U2OS cells were subjected to fluorescence-activated cell sorting (FACS) at the OHSU flow cytometry core using an Influx sorter (BD). Red label-only (i.e. ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was resuspended in HBSS 1X/FBS 2% and filtered through a 35μm cell strainer (Falcon) prior to flow cytometry analysis on BDFACS Aria II Cell Sorter (BD Biosciences). Data from flow cytometry acquisition was analyzed using the FlowJo software (version 10 ...
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bioRxiv - Immunology 2022Quote: ... recent thymic emigrated (CD4+CD127+CD31+, designated hereafter as naïve) T cells were obtained using a FACSAria cell sorter (BD Biosciences). The purity of the isolated cells was > 95% ...
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bioRxiv - Immunology 2022Quote: Ex vivo isolated T cells and in vitro-cultured T cells were incubated with Fc-block (1:250; BD Biosciences) followed by surface staining ...
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bioRxiv - Cancer Biology 2022Quote: ... Transduced 4MOSC1 cells were sorted by FACS for viability and GFP-positivity using a FACS-Aria Cell Sorter (BD Biosciences).
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bioRxiv - Biophysics 2022Quote: ... Cells expressing high levels of Life-Act GFP were sorted with BD FACSMelody cell sorter (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Cell Biology 2022Quote: ... Isolated intestinal epithelial cells (107 cells/1ml) were incubated with Mouse BD Fc Block™ (55314, BD, Franklin Lakes, NJ) for 10 minutes on ice in IESC media (DMEM/F12 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were FACS sorted for intact cells using a FSC vs SSC gate using the FACS Aria instrument (BD Bioscience). The obtained cells were washed with αMEM at 300g for 7 min ...
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bioRxiv - Developmental Biology 2021Quote: Nr5a1-GFP+ cells from E18.5 and P100 testes were then sorted by fluorescent-active cell sorting (BD FACS ARIA II), excluding cell doublets ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were grown for ∼7 days and subcloned by single cell sorting on a BD FACSAria UV instrument (BD Biosciences). Thap1-/- clones were confirmed by Sanger sequencing as described above.
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bioRxiv - Immunology 2019Quote: Single-cell capture and cDNA library preparation was performed using the BD Rhapsody Express Single-cell analysis system (BD Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and 3×106 cells per tube were stained as follows: cells were incubated in 0.5 μg Fc block (BD Biosciences) for 15 min at 4°C in staining buffer (Ca2+ - and Mg2+ - free PBS containing 3% heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: ... GFP and IgG double-positive cells (i.e. BCR-expressing cells) were sorted on a FACS ARIA-II SORP (BD Biosciences) and cultured.
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bioRxiv - Microbiology 2020Quote: ... human ES cell line WA09 was obtained from Wicell International Stem Cell Bank and cultured on Matrigel-coated (BD Biosciences) 6-well plates in mTeSR1 media (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: Sorted tumor cells were seeded at a density of 45 cells per 96/well on Growth factor-reduced Matrigel (BD) and allowed to grow for up to 6 days under standard culture conditions (See cell culture) ...
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bioRxiv - Immunology 2020Quote: ... Enriched CD4+ T cells were further sorted for either YFP+ (Foxp3+) T cells or CD25-CD44-loCD62Lhi naïve T cells by FACSAria (BD Bioscience). Purity of sorted cells was > 99% ...
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bioRxiv - Cancer Biology 2021Quote: ... Single-cell suspensions were stained with 5 μM Cell-ID Cisplatin (Fluidigm Corp.) and incubated with Fc block (BD Biosciences), followed by surface antibody cocktail ...