Labshake search
Citations for Becton, Dickinson and Company :
2301 - 2350 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... 1×105 Tconv cells were co-cultured with Treg cells at different ratios in the presence of 2×105 irradiated APCs and 1 µg/ml soluble anti-CD3 antibody (554829, BD Biosciences). The cells were cultured in T-cell complete media which consists of RPMI 1640 medium supplemented with 50 U/ml penicillin ...
-
bioRxiv - Cell Biology 2020Quote: Protein synthesis of Syndecan-1 was shown by immunocytochemistry using a polyclonal antibody against murine Syndecan-1 (CD138) clone 281-2 (1:50, rat anti-mouse, BD Pharmingen) in co-cultured cells after fixation with ice-cold 99% methanol and heparitinase III (2.4mIU/ml ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cell were resuspended in 200 μl of 2% KSR in PBS and analysed on a 5 laser LSR Fortessa analyser (BD Biosciences). Single cells were gated based on forward and side scatters ...
-
bioRxiv - Developmental Biology 2020Quote: ... 2 partly overlapping holes were punched out by using a 16-gauge Jamshidi bone marrow biopsy needle (BD Biosciences., NJ, USA). The edges of the holes were trimmed with a surgical scalpel and the bottom of the defect was curetted to expose the bleeding subchondral bone ...
-
bioRxiv - Immunology 2022Quote: ... 1–3 ×106 PBMC were stained with a saturating concentration of the following antibodies: CD3 AF700 (clone SP34-2, BD Biosciences), CD4 eFluor450 (clone OKT4 ...
-
bioRxiv - Molecular Biology 2020Quote: The SARS-CoV-2 S gene (Genbank accession no. NC_045512.2) was synthesized and cloned into a baculovirus shuttle vector pAcgp67B (BD Biosciences, CA, USA) using Gibson assembly ...
-
bioRxiv - Immunology 2021Quote: ... Samples were washed three times in ice cold PBS supplemented with 2% FBS and sorted on the FACSAria Fusion (BD Biosciences) with Ghost Dye Red 710 (Tonbo Biosciences ...
-
bioRxiv - Cancer Biology 2019Quote: ... MDA-MB-231 parental or HSF1 KO with either scrambled control or DYRK2 knock-down shRNA (1 or 2) were counted and suspended in sterile phosphate buffer saline containing 50% Matrigel (BD Bioscience). 300,000 cells (100 μl ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were stained using 8 volumes of stain solution per volume of culture (2% HCT) and analysed on a LSR-II Flow Cytometer (BD Biosciences). Statistical significance was determined by the two-sample Wilcoxon rank-sum (Mann-Whitney ...
-
bioRxiv - Immunology 2019Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
-
bioRxiv - Immunology 2019Quote: ... The surface staining was performed in PBS for 15 minutes at room temperature in the dark and subsequently fixed using Permeabilizing Solution 2 (BD Biosciences) for 10 minutes at room temperature.
-
bioRxiv - Immunology 2021Quote: ... and chemokines (CXCL-10, CCL-2, CXCL-9, CXCL-8 and CCL-5) was quantified by specific cytometric bead arrays (BD Biosciences) on a FACS Canto (BD Biosciences ...
-
bioRxiv - Genetics 2020Quote: ... Cells were subsequently washed and resuspended with cold PBS supplemented with 5% FBS and stained with 2 mg/ml Propidium Iodide (BD Biosciences) at room temperature for 10 min ...
-
bioRxiv - Immunology 2021Quote: ... and stained for 20 minutes at 4°C with 50 μl of antibody cocktail containing subset-specific antibodies (Supplementary Table 2) diluted in Brilliant Stain Buffer (BD Biosciences). Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
-
bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
-
bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
-
bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
-
bioRxiv - Immunology 2021Quote: ... B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum) followed by staining with anti-mouse CD16/CD32 (mouse Fc block; BD Biosciences) and a decoy tetramer (BV510 ...
-
bioRxiv - Microbiology 2020Quote: Candida strains (Supplementary Table S1) were routinely grown at 30°C in yeast peptone dextrose (YPD) media (2% peptone (BD Bacto), 2% D-glucose (Fisher Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... APC-R700 rat anti-mouse IL-2 (Cat No.-565186) and PE-CF594-rat anti-mouse IFN-γ were purchased from BD Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... into FACS buffer (1X PBS with 2% FBS and 1 mM EDTA) and analyzed with a FACS Aria flow cytometer (BD Biosciences).
-
bioRxiv - Immunology 2022Quote: ... Samples were washed three times in ice cold PBS supplemented with 2% FBS and sorted on the FACS Aria Fusion (BD Biosciences) with Ghost Dye Red 710 (Tonbo Biosciences ...
-
bioRxiv - Synthetic Biology 2022Quote: ... samples of 1×106 per mL pooled CAR T cells with 2×106 per mL Nalm 6 were centrifuged at 300 × g for 2 min in 96-well flat-bottom plates and incubated in HTCM with 1x Brefeldin-A/GolgiPlug (BDBiosciences #555029), 1x Monensin/GolgiStop (BD Biosciences #554724) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Written informed consent was provided by study participants and/or their legal guardians and 2-5mL of venous blood were collected into CPD vacutainer tubes (BD Vacutainer). Alternatively ...
-
bioRxiv - Cell Biology 2019Quote: Huh-7 cells were taken from the xCELLigence wells at defined times along the different proliferation curves and their position in the cell cycle was evaluated by measuring 5-bromo-2-deoxyuridine (BrdU) incorporation using the APC BrdU Flowkit (catalogue number 552598, BD Pharmingen). Cells were incubated with BrdU (10 mM ...
-
bioRxiv - Microbiology 2020Quote: ... 2 µg/ml lysostaphin, 2 mM CaCl2, 10 µl/ml annexin V-APC [BD Biosciences] and 10 µl/ml 7AAD [BD Biosciences]). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were washed twice with FACS buffer and strained through a 100 μm filter before sorting CD11b+ microglia and ACSA-2+ astrocytes on a FACS Aria III (BD Biosciences) with a 100 μm nozzle ...
-
bioRxiv - Microbiology 2021Quote: ... The cells were then washed once in a large volume of PBS and post-fixated in 2% PFA in PBS before analysis on an LSRII flow cytometer (BD Biosciences). Data was analyzed using Flowing software (version 2.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... P0-P1 mouse pups were anesthetized in ice and then eyes were pierced at the edge of corneal by 30G × 1/2 needle (BD, 305106) under stereomicroscope ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
-
bioRxiv - Immunology 2019Quote: ... Cells were washed with FACS buffer and fixed with 2% paraformaldehyde for 1hr prior to analysis on an LSRII flow cytometer (BD Biosciences). When stain sets contained tetramers ...
-
bioRxiv - Neuroscience 2019Quote: ... IL-6 and MIP-2 were measured using paired antibodies and recombinant cytokines according to the manufacturer’s recommendations (BD PharMingen Systems) (11).
-
bioRxiv - Immunology 2020Quote: ... The cells were washed once with FACS buffer and fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). In some experiments ...
-
bioRxiv - Immunology 2020Quote: ... Intracellular staining of IL-2 followed the same protocol as that for CTLA-4 but included the addition of 1 μg/mL GolgiPlug (BD Biosciences) to the culture media 12 hours prior to sample preparation ...
-
bioRxiv - Developmental Biology 2020Quote: ... AAV9-mediated Snhg1 or NC were delivered by intracardiac injection into the left ventricle in 7-day-old mice at a dose of 2 × 1010 viral genome particles per animal using an insulin syringe with a 30-gauge needle (BD Biosciences). And the adult mice received the injection at a dose of 1 × 1011 viral genome particles per animal ...
-
bioRxiv - Immunology 2020Quote: ... the following antibodies and viability dye were incubated with PBMCs at RT for 20 min: anti-CD3 BUV395 (clone SP34-2; BD Biosciences), anti-CD4 BUV495 (clone SK3 ...
-
bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
-
bioRxiv - Immunology 2021Quote: ... single MR1-5-OP-RU-tetramer+TRAV1-2+ PBMCs from healthy donors and spleen tissues were sorted using a FACSAria (BD Biosciences) into 96-well plates ...
-
bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
-
bioRxiv - Immunology 2019Quote: ... Activation assays were performed by culturing cells with recombinant human IL-2 (10ng/ml, RND Systems) in the presence of coated anti-CD3 and anti-CD28 (2mg/ml, BD Biosciences) in the culture medium ...
-
bioRxiv - Immunology 2019Quote: ... were resuspended in D-PBS supplemented with 2% (v/v) FBS containing the antibody cocktail in 96-well microtiter plates (BD Biosciences) for 20 min on ice in the dark ...
-
bioRxiv - Physiology 2020Quote: ... 1 ng/mL FGF2 and 10 mg/mL gentamycin) and plated on dishes previously coated with 2% Matrigel™ (BD biosciences).
-
bioRxiv - Cell Biology 2021Quote: ... macrophages were incubated with 5 μM 2’, 7’ dichlorofluorescein diacetate (DCFDA, Invitrogen at 37°C for 30 min, followed by acquisition using FACS Canto I (BD Sciences) within 30 minutes of staining ...
-
bioRxiv - Molecular Biology 2019Quote: ... Nuclei were passed through a 40 μm filter and stained with 2 μg/mL DAPI before sorting on a BD FACSARIA III (BD Biosciences). Aliquots of 40,000 nuclei were collected in 10X binding buffer (200 mM HEPES-KOH pH 7.9 ...
-
bioRxiv - Genomics 2020Quote: ... Cells were then incubated with 5ul human Fc Block with 95ul 2% FBS in PBS at 4°C for 15 mins before staining with SCMK and AbSeq antibodies (BD Biosciences) at 4°C for 60 min ...
-
bioRxiv - Neuroscience 2021Quote: ... The dissected tissue was mechanically triturated by a fire-polished glass pipette and plated onto 24-well plates coated with 2% laminin (BD Biosciences) and 1% poly-D-lysine (Sigma Aldrich) ...
-
bioRxiv - Pathology 2021Quote: Cells were fixed in 2% PFA for 15 minutes at room temperature and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight as previously described 36 ...
-
bioRxiv - Biophysics 2020Quote: ... and a strain with a known binding site and repressor copy number (R) - were inoculated into individual tubes with 2 mL of Lysogeny Broth (LB Miller Powder, BD Medical) with 20 µg/mL of chloramphenicol and 30 µg/mL of kanamycin ...
-
bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
-
bioRxiv - Immunology 2021Quote: ... as a negative control in the presence of αCD28/αCD49d co-Stim antibodies (1 μg ml−1) GolgiStop (containing Monensin, 2 μmol/L), GolgiPlug (containing brefeldin A, 10 μg ml−1) (BD Biosciences) and anti-CD107α BV421 antibody (BD Biosciences) ...