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Citations for Becton, Dickinson and Company :
2351 - 2400 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibody cocktail (Suppl. Table 2) for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The 24-color extracellular staining master mix (Extended Data Table 2) was prepared 2X in BD Horizon™ Brilliant Stain Buffer to prevent staining artifacts from BD Horizon Brilliant dye interactions and added 1:1 to cells ...
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bioRxiv - Cancer Biology 2020Quote: Cells either from culture or from transwell assay were washed in PBS and incubated in 2% FBS in PBS with anti-CCR7-FITC (1:25, #561271, BD Biosciences) and anti-ROR1-APC (1:25 ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured in the presence of 1 μg/mL brefeldin A and monensin (00-4505-51, eBioscience) for 2 hr and were then fixed and permeabilized with Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... The tube was then stored in the dark at 4° C for up to 2 hours prior to acquisition on the LSRII or FACSLyric analyzers (BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Lipid peroxidation was detected by measuring the fluorescence intensity of BODIPY 581/591 C11 using BD FACS Canto 2 (BD Biosciences). In each case ...
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bioRxiv - Physiology 2022Quote: ... Novus) and F(ab’)2-donkey anti-Rabbit IgG (H+L) secondary antibody PE (1:100, 12-4739-81, BD bioscience) at 4℃ for 1h ...
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bioRxiv - Synthetic Biology 2022Quote: ... thermoglucosidasius DSM2542T was routinely grown on 2-SPY medium106 at 60 °C with 150 RPM shaking or on Tryptic Soy Agar plates (BD Biosciences) at 60 °C ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-β2 mAb IB4 (10 μg/ml) followed by Alexa Fluor 488-conjugated secondary antibody and analyzed using a FACScan (BD Biosciences) and Attune NxT flow cytometer (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... with a purity >95% into sterile filtered PBS with 2% FBS or acquired on a FACS Canto II or LSR fortes Analyzer (BD Biosciences). Single cells were index sorted using single cell depositor ...
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bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 100 µL of biotinylated anti-IgG1 (1 µg/mL in PBS with 2% FBS, clone A85-1, BD Biosciences) for 2 hours at RT ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Microbiology 2024Quote: ... pyogenes M1T1 5448 strain (56) were grown aerobically at 37°C in Todd-Hewitt Broth supplemented with 2% yeast extract (THY, BD, France). Liquid and solid cultures of L ...
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bioRxiv - Immunology 2024Quote: ... Colonies were then picked for shape and hemolytic activity and cultured in Todd Hewitt Broth supplemented with 2% Yeast Extract (Fisher 50489152) or Brain Heart infusion media (BD DF0037178) in 5%CO2 at 37°C ...
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bioRxiv - Cell Biology 2024Quote: The human iPSC line MUSli001-A was generated from skin fibroblasts from a caesarean section [34] and cultured on a polystyrene plate coated with 2 µg/mL Matrigel (Growth Factor Reduced; Corning, BD Bioscience) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae strains were derived from CEN.PK113-7D and grown at 30°C in Yeast Extract Phytone Dextrose media (YEPhD, 2% Difco™ phytone peptone (Becton-Dickinson (BD), Franklin Lakes ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for a minimum of 20 minutes with 2% paraformaldehyde and acquired immediately using a FACS Symphony A3 (BD Biosciences). Where intracellular staining was performed ...
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bioRxiv - Pathology 2023Quote: ... Placentas were perfused for 3 hours and 1 ml (T4 transfer experiments) or 2 ml (TRIAC transfer experiments) samples were collected from the reservoirs into blood collection tubes (BD vacutainer) at the time points indicated in the figures ...
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bioRxiv - Immunology 2022Quote: ... The cells were fixed in 2% paraformaldehyde and GFP expression measured and quantitated using a FACS Canto II flow cytometer and FlowJo software (BD Biosciences). IC50 values were calculated using Prism software (GraphPad/Dotmatics).
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bioRxiv - Immunology 2023Quote: ... plates were incubated with serum (2 fold dilutions, 1:20-1:2560) and parasite specific antibody was measured using biotinylated IgG1 (BD Biosciences) or IgG2c (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... were stained with the specified antibodies on ice for 30 min in D-PBS supplemented with 2% FBS in the dark and analyzed using a Fortessa or Symphony A3 (BD Biosciences). Data was analyzed using FlowJo10 (Tree Star Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed with PBS three times and resuspended in FACS buffer (PBS with 2%FBS) for analysis on BD Biosciences LSR Fortessa using a 488-nm laser and 530-nm/30 filter (BD Biosciences). Data analysis was performed with FCS Express 7 (De Novo Software) ...
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bioRxiv - Bioengineering 2023Quote: ... TCR expressing cells were mixed with K562-A2 cells at a ratio of 1:2 (Effector:Target) in the RPMI media and supplemented with 1 µg/ml of anti-CD28/CD49d antibodies (BD Biosciences, 347690) and 1 µg/ml of cognate peptides ...
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bioRxiv - Biophysics 2022Quote: ... Saccharomyces cerevisiae strain W303-GAL4 was grown overnight in YPDA medium (yeast extract 1 %, adenine sulfate 540 μM, peptone 2 % (BD Biosciences), glucose 2 % ...
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bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
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bioRxiv - Biophysics 2022Quote: ... #131-8; #130-6; #130-5; #130-3 and 2 μm lot MM2307#156; #159.1; #159.2; #122.3, BD Biosciences, San Jose, CA). For calibration of the fluorescence axis in the PE channel ...
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bioRxiv - Biophysics 2022Quote: ... The FITC ERF values were assigned to both 2 μm and 6 μm beads using a specific lot of FITC-FC Bead (BD Biosciences) as a calibrator with known ERF value ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then fixed in 2% formaldehyde and stored at 4°C until flow cytometry on an LSR II flow cytometer (BD Biosciences). Cell surface markers included CD3 (clone 17A2 ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed with 2% paraformaldehyde for 20 min and resuspended in 350 ul 1% BSA/PBS for phenotyping by FACSDivacytometer (BD Biosciences). To set up the compensation control ...
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bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then selected in puromycin (1μg/ml) 2 days after transduction and sorted for their EGFP fluorescence after 6 additional days using FACS ARIA III (BD Biosciences). Cells were grown for recovery and collected for genomic DNA extraction with DNeasy Blood and Tissue kit (Qiagen).
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bioRxiv - Immunology 2023Quote: ... Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail, BD Biosciences) and added to the cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Anaerobic bacteria colonies were counted after incubating these agar plates for 2 days using the GasPak anaerobic gas generating pouch system (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... GYM (glucose 4 g, yeast extract 4 g, malt extract 10 g, CaCO3 2 g, and Bacto Agar (BD Difco™) 20 g ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and the second model investigated ii) four exposures from the first eating event (AK-20, BD, KB, LT & NH; Model 2). In each model ...
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bioRxiv - Immunology 2023Quote: ... 1 μg/ml each of ESAT-6 and CFP-10 peptide pools (provided by Aeras, Rockville, MD) or R10 media for 2 h before adding 10 μg/ml BD GolgiPlug (BD Biosciences) for a further 12 hours ...
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bioRxiv - Immunology 2023Quote: ... prior to stimulation for 5 h with anti-CD3 and anti-CD28 without IL-2 in the presence of brefeldin A and monensin (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... The tube was then stored in the dark at 4°C for up to 2 hours prior to acquisition on a LSRII or FACSLyric analyzer (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
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bioRxiv - Cell Biology 2023Quote: ... cells were suspended in 350 µL of FACS buffer (1XDPBS, 2% FBS, 25mM HEPES, 2mM EDTA) and was analysed by flow cytometry BD LSRFortessa (BD Biosciences). 10,000 cells were analysed for each condition ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 – 2 mL of thin albumin was aspirated out with a 5 mL syringe (Nipro) and a 18G sterile needle (BD PrecisionGlide) from the blunt end of the egg to create a space between the embryo and the shell ...
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bioRxiv - Bioengineering 2024Quote: ... for viability assessment and fixed with 2% paraformaldehyde (30 min incubation) until analysis by flow cytometry using an LSR Fortessa (BD Biosciences). For assessment of NP-associated fluorescence in a fluorescence plate reader ...
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bioRxiv - Immunology 2024Quote: T cells were restimulated with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 or PMA/Ionomycin in the presence of GolgiPlug protein transport inhibitor (BD Biosciences) for 6 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Isolates from the CNRMA in France were subcultured on 2% malt extract agar (Oxoid) and potato dextrose agar (BD Diagnostic Systems) for 5 days at 30 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were sorted for mCherry and EGFP fluorescence using a BD FACSAria™ Cell Sorter (BD Biosciences, San Jose, CA), after 48 h of transduction ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody incubations were performed for 15 minutes on ice before sorting the cells using a FACSAria II cell sorter (BD).