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Citations for Becton, Dickinson and Company :
2401 - 2450 of 2467 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 2000 cells were plated in 8-well chamber slides onto a base layer of growth factor-reduced Matrigel (5 mg/ml, BD Biosciences, Lot A6532) and collagen type I (1.5-2.1 mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Assay plates were incubated overnight at 37ºC in a 5% CO2 incubator and developed using BD ELISPOT AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISPOT reader and Immunospot software (Cellular Technology ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked in PBS + 5% BSA for 1 hour before addition of primary antibodies (anti-LC3A/B, CST #4108, 1:100; anti-LAMP1, BD #555798, 1:100) diluted in blocking buffer overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, New Jersey, USA). Unless otherwise stated ...
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bioRxiv - Microbiology 2021Quote: ... or LB media (10 g/liter Bacto tryptone, 5 g/liter Bacto yeast extract, 10 g/liter sodium chloride; BD, San Jose, CA) at 30°C ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with PerCP/Cy5.5 or BV421-conjugated anti–mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Off-line samples were taken from the reactors each 5-10 h and the analysis of the GFP expression levels was performed with a BD Accuri C6 (BD Biosciences, CA, USA) based on FL1-A channel (excitation 488 nm ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... the BECs were seeded in 96-well plates coated with collagen type I (5 µg/mL, #354231, BD Bioscience, San Jose, CA, USA) at a density of 104 cells per well ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were stimulated with the RBD peptide pool (5 μg.ml−1 final concentration) and brefeldin A (1 μg.ml−1 ; BD Pharmingen, San Diego, CA, USA) at 37 °C overnight ...
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bioRxiv - Immunology 2022Quote: Surface staining: Single cell suspensions were incubated with 5% normal rat serum and rat anti-mouse CD16/CD32 antibody (BD Biosciences close 2.4G2) then stained with fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of detection antibody (IFNγ, BD Biosciences, #51-1818KA; IL-17A, Invitrogen, #88-7371-88; and IL-5, BD Biosciences, #51-1806KZ) was added at a 1:250 dilution in ELISpot diluent (eBioscience ...
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bioRxiv - Genomics 2024Quote: ... 2.5 x 105 cells were stained for 75 minutes at room temperature with PE Mouse anti-PDX1 Clone-658A5 (BD Biosciences; 1:10) and Alexa Fluor® 647 Mouse anti-NKX6.1 Clone R11-560 (BD Bioscience ...
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bioRxiv - Microbiology 2024Quote: The urine sample obtained from the patient was quantitatively inoculated onto a Columbia agar plate with 5% sheep blood (BD Diagnostics, Heidelberg, Germany) and a MacConkey II-Agar plate (BD Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed three times with PBS for 5 min each and then analyzed by flow cytometry (LSRFortessa, BD Biosciences, MD, USA). The mean fluorescence intensity (MFI ...
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bioRxiv - Biophysics 2024Quote: ... After 5 or 10min of incubation at room temperature (including the 1min centrifugation) cells were fixed with BD fixation buffer (BD Biosciences 51-2090KZ) for 30min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... PerCP-Cy5.5-conjugated anti-IFN-γ (Clone 4S. B3, 1:10), and PE-cy7-conjugated anti-TNF-α (Clone Mab11, 1:50) (BD Biosciences) for intracellular staining ...
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bioRxiv - Physiology 2023Quote: ... p = 0.2 (sum of shared targets between 1/0 and 5/0 R-βHB ex vivo groups and BH-BD targets, divided by n), and q = 0.8 ...
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bioRxiv - Immunology 2023Quote: ... was added to the cell suspension and Fluorescein isothiocyanate (FITC)-positive to FITC-negative cell percentage was measured after 5 min of incubation using the BD FACSCanto™ Flow Cytometer (BD Biosciences, USA). Results were analyzed using the Flowing Software (version 2.5.1 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Immunology 2023Quote: ... and the cells were stained with antibodies specific for the following proteins: CD4 (PE Rat Anti-Mouse CD4; Clone RM4-5; BD Biosciences; Cat # 553049), H-2Dd (Anti-mouse H-2Dd antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% skim-milk diluted in 1X TBST and incubated with primary antibodies overnight [RB (BD Pharmingen #554136, 1:500), p53 (Pierce #MA5-12453 ...
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bioRxiv - Cell Biology 2024Quote: ... A master mix (5 µL of each fluorochrome-conjugated antibody + 10 µL of Brilliant Staining Buffer (Figure 1 a & b) (BD Horizon™ #659611) or Super Bright Complete Staining Buffer ...
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bioRxiv - Genetics 2024Quote: ... Cells were stained with 5 ml PE Mouse Anti-Cardiac Troponin T antibody (564767, clone 13–11, BD Biosciences, San Jose, CA, USA) for 45 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... jejuni grown on MH agar were resuspended in 5 ml of MH broth in a 14-ml round-bottom tube (BD Falcon, MA, USA) to an optical density at 600 nm (OD600 ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were incubated with phycoerythrin (PE)-conjugated Enpep antibody at 4°C for 60 min and then sorted by FACScan flow cytometry (Becton, Dickinson and company, Franklin Lakes, NJ).
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bioRxiv - Immunology 2020Quote: ... Cells were then surface stained for 30 min in the dark at 4°C with viability reagent (BD Horizon™ Fixable Viability Stain, BD Biosciences, San Diego, CA, USA) and a 29-color cocktail of monoclonal antibodies (mAbs ...
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bioRxiv - Immunology 2024Quote: ... Non-specific antibody staining was inhibited using blocking buffer (male 10% human AB serum, 1% bovine serum albumin, 2 mM EDTA, 25 μg BD Fc Block; 15 min, 4°C) and washed before completing surface stain in staining buffer (PBS supplemented with 1% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... All tubes were centrifuged for 4 minutes at 2,000 RPM and the cells were resuspended in 200 μL of Antibody buffer (BD Perm/Wash buffer + 10 μL goat serum) with or without BRN3A antibody (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... MM1.S cells incubated overnight in the presence of Cy5-labeled DNA ODN were fixed with 4% paraformaldehyde and then labeled with anti-LAMP1 antibody (BD, cat no. 555798, 1:100 dilution) followed by staining with 1 µg/mL DAPI.
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bioRxiv - Cancer Biology 2022Quote: ... The co-cultures were incubated for 6 hours at 37°C and 5% CO2 prior to cell staining using the Cytofix/Cytoperm Fixation Kit (BD Biosciences, Cat# 554715/555028).
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bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
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bioRxiv - Neuroscience 2020Quote: ... infected mouse brain for flow cytometry were obtained as described.(Posel et al., 2016) Isolated cells were incubated overnight (5 hours for microglia analysis) in GolgiPlug and GolgiStop (BD Bioscience #555029 and #554724) in RPMI media containing HEPES and 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were resuspended in PBS + 5% FBS and analyzed via Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Fluorescence minus one (FMO ...
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bioRxiv - Microbiology 2020Quote: ... or 0 to 10−2 fluorescent intensity (AF647-negative) were captured and segregated in 2 separate 5 ml round-bottom tubes (BD Biosciences, San Jose, CA) containing 1% BSA/PBS ...
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bioRxiv - Microbiology 2021Quote: Lorikeet intestinal contents collected at necropsy were plated on reduced Trypticase Soy Agar (TSA II) with 5% sheep blood (BD BBL, Franklin Lakes, NJ) and incubated anaerobically (5% CO2 ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were cultured in complete media (106 cells /ml) for 72h in anti-CD3/anti-CD28 (5 μg/mL; BD Biosciences, San Jose, USA) pre-coated 96-flat-well plates (BD Biosciences) ...
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bioRxiv - Genomics 2020Quote: ... at a concentration of 100μg/ml starting 5 days after transfection for the following 7-9 days before single cell sorted using BD FACSAria II (BD Bioscience, San Jose, CA) for clonal cell line generation ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
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bioRxiv - Microbiology 2021Quote: ... This was done by treating both the PDMS and glass surface with 5-10 sweeps of a portable laboratory corona device (model BD-20ACV, Electro-Technic Products). After treatment the chip was placed carefully onto the glass slide and gently tapped to facilitate full contact between the PDMS and glass surface ...
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bioRxiv - Immunology 2021Quote: ... The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A) (BD Biosciences, San Jose, CA) at a final concentration of 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... the frozen bacterial stock was thawed in 37°C water bath and cultured at 37°C in 5% CO2 in Todd Hewitt broth (BD Biosciences, San Jose, CA) to mid-logarithmic phase ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with ice-cold DPBS plus 5 mM EDTA and stained with annexin V and PI (BD Biosciences, Franklin Lakes, NJ, USA) for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... 1.5 µg/mL) [31] in the presence of anti-CD28 and anti-CD49d antibodies (both at 1 μg/ml, BD, Franklin Lakes, New Jersey) and brefeldin-A (10 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... namely anti-CD3-APC (1:5 dilution, clone SK7) and anti-CD45-PerCP (1:10 dilution, clone 2D1) (both from BD Biosciences, San Jose, CA). After incubation for 30 min at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Developmental Biology 2023Quote: ... cells filtered into 5 ml polystyrene tubes and analyzed by flow cytometry using a FACS Caliber flow cytometer and FlowJo software (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked in 5% non-fat dry milk in TBS/Tw and then incubated overnight with primary antibody Syn-1 (BD Biosciences #610,787, 1:1000) or MJFR-14-6-4-2 (Abcam #209,538 ...
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bioRxiv - Genetics 2024Quote: ... the pigs were fasted overnight and 5∼10 mL blood was sampled from the jugular vein using a vacutainer (Cat no. 367986, BD Vacutainer SST™, USA) and transported to the laboratory within 10 min ...