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Citations for Becton, Dickinson and Company :
2301 - 2350 of 2467 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cultured pMacs in 24-well plates were incubated in primary anti-MHC-II monoclonal antibody (mAB; BD Biosciences, see Table 5) at a final concentration of 5μg/mL for 1 hour at 37°C in FACS buffer to allow for the antibody-binding and subsequent uptake of MHC-II complexes ...
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bioRxiv - Microbiology 2024Quote: ... and its mutants were all grown at 37°C with 5% CO2 with either Todd-Hewitt medium (TH medium; BD Biosciences), chemically defined media (CDM) ...
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bioRxiv - Immunology 2024Quote: ... and WT littermate controls (n=5) were stained as described above with anti-CD3e FITC (clone 145–2 C11, BD Biosciences), anti-CD4 AF700 (RM4-5 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in 1X PBS with 1X protease/phosphatase inhibitor cocktail in and filtered through a 5 ml cell-strainer capped FACS tube (BD Falcon). Cell sorting was performed on BD Biosciences FACS Aria Fusion ...
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bioRxiv - Immunology 2024Quote: ... and each tube was stained with 5 μL of APC-Cy7 Mouse Anti-Human CD31 (PECAM-1) antibody (clone WM59, BD Biosciences) and 5 μL of APC anti-human CD144 (VE-Cadherin ...
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bioRxiv - Molecular Biology 2024Quote: ... were stimulated by incubation with 5 µg ml-1 anti-IgM F(ab’)₂ fragment antibody (115-006-020; Jackson Immuno Research) with or without 5 µg ml-1 anti-CD40 antibody (553787, BD Pharmingen), with 100 ng/ml MPL-A (Invivogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Xenografts were established by subcutaneous injections of PC-9 cells expressing ERBB4 variants (5 x 106 cells in 50% Matrigel matrix (#354234, BD Biosciences)) in the right flank of each mouse ...
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Effective targeting of CD19 positive primary B-ALL cells using CAR-NK cells generated with mRNA-LNPsbioRxiv - Cancer Biology 2024Quote: ... NK cells were transfected with 1-2μg EGFP mRNA-LNP per 106 cells on day +7 in upright culture flask at 37°C in a humidified incubator with 5% of CO2 and then harvested for flowcytometry analysis with BD FACSLyric system (BD Biosciences). Optimal transfection conditions were determined using EGFP mRNA-LNP expression results ...
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bioRxiv - Cancer Biology 2024Quote: ... and monocyte block for 5 minutes prior to staining with the 20-antibody cocktail (Supplemental Methods) in Brilliant Stain Buffer Plus (BD Biosciences) and FACS buffer (PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was taken after 5 hours (day 0) and strain ratios were immediately measured using a flow cytometer (HTS, Fortessa, BD Bioscience). Cultures were then propagated with 1:1000 dilutions into fresh medium every 24 hours for other 2 days ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 4% milk for 1 hour and incubated overnight at 4°C with shaking in the appropriate primary antibody against Nischarin (1:500, BD Biosciences, Cat No: 558262), GFP (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Cochlear whole mounts were immunostained overnight at 4°C with primary antibody against CTBP2 (mouse anti-CTBP2 at 1:200; BD Transduction Labs, Heidelberg, Germany). Secondary antibody (donkey anti mouse conjugated with Cy3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated overnight at 4°C in solution containing 1% BSA and primary antibodies: mouse F4/80 (T45-2342, BD Biosciences, New Jersey, USA) 1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated as described above and stained at 4°C (15 min) with fluorochrome-conjugated antibodies against surface markers of endothelial cells (CD31 (BD Biosciences, Franklin Lakes, USA) and myeloid cells (CD45 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... surface stained, fixed with 4% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). Antibodies used for flow cytometry included anti-human CD3 AF700 (clone UCHT1) ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Immunology 2022Quote: ... the cells were fixed with 2% PFA and stored at 4°C until the samples were acquired on a LSRII cytometer (BD Biosciences, Mississauga, ON, Canada). Data analysis was performed using FlowJo v10.5.3 (Tree Star ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed and permeabilized for 30 min at 4 °C in the dark after extracelluar stainings using BD Cytofix/Cytoperm (Fixation and Permeabilization Solution, BD Biosciences, Cat: 51-2090KZ) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... For the induction of inflammatory responses PD fluid treated mice received intra-peritoneal injections of 400 μL 4% Brewer modified thioglycollate medium (BD Biosciences, San Jose, CA) or sterile saline as control ...
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bioRxiv - Cell Biology 2022Quote: ... Cells grown on 96 well plates were fixed and permeabilized for 20 mins at 4°C with Fixation/Permeabilization solution (BD Biosciences, cat. no. 554714). Cells were washed 3 times in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... The stained cell suspensions were fixed in 4% paraformaldehyde and resuspended in PBS (1% BSA) before flow cytometry analysis using a FACSymphony™ A3 cell analyzer (BD Biosciences, NJ, U.S.A.).
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed in 4% paraformaldehyde and permeabilized using a commercially available saponin-based permeabilization and blocking reagent (1x BD Perm/Wash buffer #554723). Cells were then stained using primary antibodies against Flag (Proteintech 20543-1-AP ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were washed 5x times with TBS-T and stained overnight at 4°C with gentle agitation with primary antibodies: mouse anti-rat CD63 (1:200 dilution, BD Biosciences, Mississauga, ON, Canada), rabbit anti-rat CD9 (1:500 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4°C with primary antibodies: ALIX (BD Biosciences, 611620, 1:1000), calnexin (Santa Cruz ...
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bioRxiv - Immunology 2024Quote: ... Sections were incubated overnight at 4°C with the following primary antibodies: anti- CD3e (T-cell marker, Hamster Anti-Mouse CD3e 500A2, catalog #553238, BD Biosciences, Franklin Lakes, NJ), anti-IgD (follicular B cell marker ...
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bioRxiv - Immunology 2024Quote: ... Cells were then permeabilized for intracellular staining with the Foxp3/Transcription Factor Staining Buffer Set (eBioscience, San Diego, CA) at 4°C overnight and then stained with Brilliant Ultra Violet anti-mouse CD69 (BD Biosciences, Franklin Lakes, NJ), PE/Cyanine7 anti-mouse IL-17A (Biolegend) ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Antibody staining was carried out for 1 hour at 4°C on rotation with the following antibodies: PAX6-Alexa Fluor 488 (BD Biosciences,1:20 dilution), EOMES-PE-Cy7 (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were incubated for 4 h and washed twice with PBS for analysis of fluorescein positive cells via flow cytometer (BD FACSCantoTM II, BD Biosciences). To further investigate the internalization mechanism of PoLixNano ...
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bioRxiv - Cancer Biology 2024Quote: ... The permeabilized cells were washed three times with PBS and incubated overnight at 4 °C with anti-E-Cadherin (610182, BD biosciences, dilution 1:100), anti-vimentin antibodies (ab92547 ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each pool) and brefeldin A (1 μg/ml; BD Pharmingen, USA) at 37°C for 12-16 hours ...
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bioRxiv - Immunology 2022Quote: ... distasonis D13 strain and Bacteroides fragilis (ATCC® 25285) were cultured in anaerobic culture broth (Tryptic Soy Broth supplemented with 5 µg/ml Hemin (BD Biosciences) and 1µg/ml Vitamin K1 (VWR ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies (CD45-APC BD Biosciences 559864, and CD11b-BV421 BD Biosciences 562605) for 30 min at 4°C in incubation buffer (0.5% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies (CD45-APC BD Biosciences 561018, and CD11b-BV421 BD Biosciences 562605) for 30 min at 4°C in FACS buffer (0.5% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies for CD45-APC (BD Biosciences 561018) and CD11b-BV421 (BD Biosciences 562605 ...
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bioRxiv - Immunology 2021Quote: ... 106 cells were stimulated with 1µg/mL CMVpp65 overlapping peptides (JPT Peptide Technologies, Germany) in the presence of Brefeldin A (GolgiPlug (final concentration 5 µL/mL, BD Biosciences, USA) and Monensin (GolgiStop (final concentration 3.25 µl/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Then cells were blocked by PBS plus 5% BSA and later incubated with FITC Anti-Human CD24 (BD Biosciences, San Jose, CA), and PE Anti-Human CD44 (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... was plucked from each MOX plate and added to 5 mL of Brain Heart Infusion (BHI; BD Bacto, Franklin Lakes, New Jersey) broth and incubated at 25°C in a shaking water bath for 24 h ...
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bioRxiv - Biophysics 2020Quote: Spectrin in neurons (Figure 2d and Supplementary Figures 2 and 5) was labelled with a mouse monoclonal primary antibody anti-β-Spectrin II (Clone 42/B-Spectrin II, BD Biosciences) for 1 h at room temperature using a 1:400 dilution in 5% BSA in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... supernatant and attached cells were collected and stained with DAPI for 5 min before acquisition in BD FACS Canto II flow cytometer (BD Biosciences, USA). Results were analysed using the BD FACS DIVA (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 cells were added to the top chamber of 24-well transwells (8 μm pore size membrane; BD Bioscience, Bedford, MA), and assay medium was added to the bottom chambers and incubated for 24 hours (10% FBS) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were taken from each bottle in an anoxic chamber (95% N2 and 5% H2; Grass Lake, MI, USA) using a 23-gauge needle (BD PrecisionGlide™) and acid-washed 1 mL plastic syringe ...
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bioRxiv - Microbiology 2022Quote: ... from each bottle in replicate in an anoxic chamber (95% N2 and 5% H2; Coy Laboratory Products, Grass Lake, MI, USA) using a 23-gauge needle (BD PrecisionGlide™) and 1 mL plastic syringe ...
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bioRxiv - Cell Biology 2022Quote: ... in SIH defined minimal medium (Formedium, Norfolk, England) or on SM/5 agar (2 g glucose, 2 g bactopeptone (BD, Sparks, MD), 0.2 g yeast extract (Hardy Diagnostics ...
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bioRxiv - Neuroscience 2020Quote: ... centrifuged 500 x g for 5 min before red blood cells were lysed in 1X PharmLyse (BD Biosciences, San Jose, CA USA) for 8 min at room temperature (RT) ...
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bioRxiv - Immunology 2020Quote: ... Bacteria from cryo stock were resuspended in Brain Heart Infusion (BHI) medium and put onto blood agar plates (Columbia agar with 5 % sheep blood, BD, Cat: 4354005). Plates were incubated in a chamber with anaerobic conditions (83 % N2 ...
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bioRxiv - Immunology 2020Quote: ... Cellular depletion in spleens and intraepithelial lymphocyte compartments was monitored by flow cytometric detection with α-CD4 BV650 (clone RM4-5, BD Biosciences) or α-CD8β (clone H35-17.2 ...
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bioRxiv - Bioengineering 2020Quote: Seven days after electroporation a total of 5 × 106 T cells were collected and stained in 100 uL with fluorophore-conjugated anti-human antibodies for CD3 (BD Biosciences #564001), beta-2-microglobulin (BioLegend #316306 ...
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bioRxiv - Microbiology 2022Quote: ... jejuni on MH agars were resuspended in 5 ml of MH broth in a 14 ml round bottom tube (BD Falcon, USA) to an optical density at 600 nm (OD600 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 were added to the top chamber of 24-well Boyden chambers (8 μm pore size membrane; BD Bioscience, Bedford, MA), and medium was added to the bottom chambers and incubated for 24 hours (DMEM 10% FBS) ...