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Citations for Becton, Dickinson and Company :
2151 - 2200 of 2467 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... according to manufacturer’s instructions to exclude dead cells and then labelled in FACS buffer with the following antibodies: V500 Rat anti-Mouse CD4 (RM4-5, BD), eF450 CD5 Monoclonal Antibody (53-7.3 ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue suspension was filtered through a 5 mL Round Bottom Polystyrene Test Tube with Cell Strainer (BD Flacon, 352235), and the cell suspension obtained by filtration was fixed in 1% formaldehyde/DMEM buffer for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 120 µL of a cell suspension in McCoy’s (+10 % FBS) containing 5 x 104 cells/mL were seeded into black 96-well imaging plate (BD Falcon). The plates were incubated for two days (37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... UK), mouse monoclonal PARP (1:1000, #556494, clone 4C10-5) and mouse monoclonal cytochrome-c (1:1000, #556433) from BD Pharmingen (Heidelberg ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes were spun at 300 × g for 10 minutes and then subjected to Red Blood Cell lysis (RBC) using 5 ml of BD Pharmalyse (BD). After 5 minutes of incubation ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour and probed overnight at 4°C with the following primary antibodies diluted in 5% non-fat dry milk/TBS-T: mouse monoclonal anti-p150 clone 1/p150Glued (BD Transduction Laboratories 610473 ...
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bioRxiv - Genomics 2020Quote: ... TFA4 strain was cultivated in nutrient Zobell medium (4 g L−1 peptone and 1g L−1 yeast extract Becton, Dickinson and Company, Sparks ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were allowed to recover for 2 days in 6-well plates filled with 4 ml medium per well before being FACS sorted for mCherry-expression on a BD FACS Aria III (BD Biosciences, Heidelberg, Germany). For subsequent limiting dilution cloning ...
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bioRxiv - Immunology 2021Quote: Control and SC-macrophages were fixed with 4% PFA for 10 mins and permeabilized with 1x perm wash buffer (BD cat#51-2091KZ) for 30 mins at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were incubated overnight at 4 °C with primary mouse monoclonal anti-alpha synuclein antibody (Cat no. 610787; BD Biosciences; 1:200) and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no ...
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bioRxiv - Immunology 2022Quote: ... were added to the ELISpot plate containing an immobilized primary antibody to either IFN-g or IL-4 (BD Cat# 551881 and BD Cat# 551878, respectively), and were exposed to various stimuli (e.g ...
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bioRxiv - Microbiology 2021Quote: ... a 10 µl inoculating loop was used to streak cecal slurry (cecal contents in BPW) onto Xylose Lysine Tergitol-4 agar (BD Difco, Sparks, MD). supplemented with 4 ppm tetracycline for trial 1 and BGS supplemented 32 ppm ampicillin (amp ...
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bioRxiv - Immunology 2020Quote: ... Pellets were then stained with a panel of antibodies for 30 minutes (4°C) followed by washes in PBS before analysis by flow cytometry (BD, Franklin Lakes, NJ).
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Immunology 2020Quote: ... Cells were then spun down at 500 × g for 5 min at 4°C and resuspended with the following antibody-conjugated fluorophores for 20 minutes at room temperature: anti-CD3-BV421 (BD, 1F4, 1:100), anti-CD4-PE-Cy7 (BD ...
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bioRxiv - Systems Biology 2021Quote: ... HeLa and RAW 264.7 cells were washed by chilled PBS and sorted on the nanoPOTS chips (4 × 12, 1.2 mm diameter per well) using the Influx II cell sorter (BD Biosciences, San Jose, CA) as described previously 14 ...
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bioRxiv - Immunology 2020Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... overnight and then stimulated with 1 nM N4 in the presence of vehicle or Compound 182 (1 μM) for 4 h at 37°C in complete T cell media supplemented with BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were then stained with fluorophore-conjugated antibodies to cell surface CD8 and intracellular IFNγ ...
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bioRxiv - Immunology 2023Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Microbiology 2022Quote: ... the individual co-cultures were 10-fold serially diluted with 1X anaerobic phosphate buffer saline (PBS) and plated on Xylose Lysine Tergitol 4 (XLT4) agar (BD Difco, Houston, TX). The plates were incubated aerobically at 37°C for 24 hours followed by plating on XLT4 agar and colony forming units (CFU ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667, 1:100 in 1X BD Perm/wash solution) or anti-BST2 (Proteintech #13560-1-AP ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was washed with 3-4 ml washing buffer which was fresh PBSB 1% and then was filtered through a 70-µm cell strainer (BD Bioscience, NY, USA). Then using a long tube ...
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bioRxiv - Genomics 2023Quote: ... and half of the remaining chromatin was rotated overnight at 4°C with 4.5 µg of anti-p53 (BD Biosciences #554293, Lot #9046678). The other half of the remaining chromatin was also rotated overnight as a no-antibody control ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... mice were euthanized and tumors were quickly harvested and fixed for 14-16 hr at 4&C in BD Cytofix/Cytoperm (BD Bioscience, Cat #: 554722) diluted 1:4 in PBS ...
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bioRxiv - Immunology 2022Quote: ... In-between incubation steps, cells were washed (300 x g, 10 min, 4°C) with 40 mL of BD Cell Wash (BD Biosciences, Allschwil, Switzerland). In order to block Fc receptors ...
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bioRxiv - Microbiology 2022Quote: ... Afterwards the samples were fixated and permeabilized for 20 min and 60 min at 4 °C in the dark using BD Cytofix/Cytoperm™ solution and BD Perm/Wash™ solution (BD Biosciences), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Immunology 2024Quote: ... TCR-engineered T cells containing 5×104 transgenic TCR (mTRBC)+ cells were co-cultured with 5×104 peptide-pulsed K562 cells for 4 h at 37°C in cRPMI and in the presence of 1 μL/mL GolgiPlug™ (BD Biosciences, 555029). As a positive control ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and incubated in following antibodies for 30 minutes at 4°C in the dark: PerCP rat anti-mouse CD45 (557235, BD Biosciences, 1:50), FITC rat anti-CD11b (553310 ...
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bioRxiv - Bioengineering 2024Quote: ... 1:20 BD Pharmingen™ Alexa Fluor® 488 Mouse anti-SSEA-4 Clone MC813-70 (RUO) (BD Biosciences, Franklin Lakes, NJ, USA) 2 ml/well solution ...
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bioRxiv - Bioengineering 2024Quote: ... for 60 min at room temperature and then stained with anti-human/mouse phospho-STAT3-AF647 (clone 4/P-STAT3, BD Biosciences, 1:100) or anti-human/mouse phospho-SMAD2/3-AF647 (clone 072-670 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cell samples were stained with the following antibodies in cell staining buffer for 40 min at 4 °C: anti-mouse-CD3e-FITC (553061, BD Biosciences, 1:200), anti-mouse-CD4-PercpCy5.5 (550954 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were re-suspended in 150 μl of PBS/5% FBS and analyzed on a BD FACS Canto2 flow cytometer (BD Biosciences) for PE-Cy7 expression ...
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bioRxiv - Cell Biology 2020Quote: ... was prepared the day before the experiment by incubating the wells with 50μl/well of 5 μg/ml of anti-integrin α5 antibodies (BD Biosciences, 555651) in 0.05m Na2CO3 pH 9.6 ...
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bioRxiv - Cell Biology 2020Quote: ... The CSCs were selected as the top 5% cells with the highest GFP intensity (Oct4hi) and then sorted with the FACSAria II cell sorter (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each peptide) and brefeldin A (1 μg/ml; BD Pharmingen) at 37°C for 12-16 hours ...
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bioRxiv - Genetics 2021Quote: ... and non-neuronal (NeuN−) nuclei were sorted into tubes pre-coated with 5% BSA using a FACSAria flow cytometer (BD Biosciences) equipped with a 100μm nozzle ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples (10,000 events per sample) were analyzed after 5-10 min incubation in the dark on ice on a FACSCalibur flow cytometer (BD Biosciences) in the FL3 channel.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in 1X PBS to remove excess stain with a centrifugation step of 5 min at 2000 ×g and run on a BD FACSCanto cytometer (BD Biosciences). Based on the previous flow cytometry data for establishment of threshold values for gating (Kwon and Smith ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell were resuspended in 200 μl of 2% KSR in PBS and analysed on a 5 laser LSR Fortessa analyser (BD Biosciences). Single cells were gated based on forward and side scatters ...
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bioRxiv - Molecular Biology 2021Quote: ... the top 5% cells expressing the highest levels of CasRx-GFP were sorted using BD FACS ARIA II (BD Bioscience, USA). The CasRx-GFP expressing cells were expanded and screened for CasRx expression using qPCR (primers listed in Supplementary Table S1).