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Citations for Becton, Dickinson and Company :
2201 - 2250 of 10000+ citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Transfected cells were selected with 5 μg/mL puromycin and RFP+ cells were collected using fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
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bioRxiv - Cell Biology 2020Quote: ... The purity of the cells was confirmed by staining cells with PE-CD71 and allophycocyanin (APC)-Ter119 antibodies (all from BD Biosciences) by flow cytometry ...
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bioRxiv - Plant Biology 2021Quote: ... DNA containing cells (DRAQ5 positive) that were sorted with a 130 µm nozzle using a High speed Influx Cell Sorter (BD Biosciences). Sorted protoplasts were harvested in W5 solution (154 mM NaCl ...
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bioRxiv - Physiology 2021Quote: ... Alveolar epithelial cells were identified as CD45-CD31- T1α + and endothelial cells were identified as CD45-CD31+T1α- We analyzed cells by flow cytometry (LSR II, BD Biosciences) and using standard software (FlowJO ...
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bioRxiv - Immunology 2021Quote: ... patient lymph nodes cell suspension (around 30×106 cells/well in a U-bottom 96 well plate; BD Falcon, Cat# 3077) were washed once with 200 mL FACS buffer (4% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of AXL-negative and AXL-positive cells were done by Fluorescent Activated Cell Sorting performed on the Aria II (BD Biosciences). For sorting cells from tumor ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 μL of the cell suspension (more than 1×105 cells) was transferred to a 5 mL FACS tube (BD Biosciences) and stained in duplicate with 10 μL of FITC Annexin V conjugate and 10 μL of propidium iodide (PI ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescence Activated Cell Sorting for GFP+ cells or CD71- and Ter119-fractionation was conducted on a FACSAria II (BD Life Sciences). Data was analyzed with FlowJo v10.6.2.
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bioRxiv - Cell Biology 2021Quote: ... Cells are washed and filtered with a BD Falcon 12×75–mm tube with a cell strainer cap (BD Biosciences, 352235) and analyzed by flow cytometer ...
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bioRxiv - Pathology 2021Quote: ... CD11b) and Sca1+/c-Kit+ cells in lineage negative cells determined via flow cytometry on a BD LSRII instrument (BD Biosciences). Data was analyzed on FlowJo software (Treestar) ...
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bioRxiv - Neuroscience 2021Quote: ... Purified bone marrow cells were centrifuged at 1500 rpm for 3 min and filtered through a 100 μm cell strainer (BD Biosciences). The bone marrow cells were plated onto a 100 mm culture dis h or six-well plate (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... and NLF-shNT or -shPRELP (0.5×106 cells) were suspended in 20 µl of cell culture medium and growth-factor-reduced Matrigel (catalog no. 356230; BD Biosciences) 1:1 and co-injected into the liver of six-week old athymic nude mice (n=5 per condition).
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bioRxiv - Cell Biology 2019Quote: ... Primary hMPCs were sorted using fluorescence activated cell sorting with fluorescently-conjugated antibodies to cell surface antigens specific to hMPCs [CD56 (NCAM, BD Pharmingen) and CD29 (β1-integrin ...
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bioRxiv - Immunology 2021Quote: Mouse spleens were harvested on D105 after vaccination and single-cell suspensions were prepared by processing them through a 70-μm cell strainer (BD Biosciences). Cells were then incubated in FACS tubes at 6×105 cells per tube and stimulated for 18 hours with 2 μg/ml RBD peptide mix (PepMix SARS-CoV-2 (S-RBD ...
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bioRxiv - Immunology 2020Quote: ... PD-L1low and non-B cells from the splenocytes population was carried out by cell sorting in a FACSAria II (BD Biosciences) using different gates strategies (see Supplementary Fig.1).
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bioRxiv - Immunology 2020Quote: Surface staining of single-cell suspension of isolated brain immune cells was performed using standard protocols and analyzed on a FACSCanto II (BD Biosciences). Flow cytometric analysis was defined based on the expression of CD11b ...
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bioRxiv - Immunology 2021Quote: Naïve CD4+ T cells were defined as CD4+ CD8- CD25- CD44- CD62L+ cells and isolated on an Aria II (BD Biosciences) cell sorter ...
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bioRxiv - Immunology 2021Quote: ... stained cells were resuspended in 1% paraformaldehyde (PFA) and acquired using Stained cells were acquired on a BD LSRFortessa (BD Biosciences) and analysis performed using FlowJo v10.0.8 (Tree Star ...
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bioRxiv - Immunology 2020Quote: ... from uninfected mice and CD4+ CD25+ splenic T cells (Treg) from infected control or IWP-L6-treated mice were obtained by cell sorting with a FACSAria II (BD Biosciences). Teff cells were labeled by 5 min with CFSE (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Resting- or in vitro-generated effector CD4+ or CD8+ T cells (105 cells/well) were stimulated with 1 μg/ml plate-bound anti-mouse CD3 mAb (BD Bioscience) in combination with different concentrations of P3CSK4 ...
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bioRxiv - Immunology 2021Quote: ... and then further sorted by gating CD11c+ B cells (CD19+CD11c+CD21-) and CD11c- B cells (CD19+CD11c-CD21+) using FACSAria II (BD Biosciences). Detailed information of antibodies were listed in Supplementary Table 4
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bioRxiv - Immunology 2020Quote: ... CD138 (plasma cell marker) and other B cell surface molecules on a flow cytometer (BD LSR II™ or Celesta™). To analyzed immune cells in vivo ...
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bioRxiv - Immunology 2020Quote: ... We only used iTreg cells for experiments if samples achieved at least 80% Foxp3 positive cells (00552300, Foxp3 Staining Kit, BD Bioscience).
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bioRxiv - Immunology 2021Quote: ... mice spleens were then harvested and Batf cKO and Irf4 cKO CD8+ P14 T cells were sorted with cell sorter (BD, FACSAria) according to the GFP and YFP fluorescence expression ...
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bioRxiv - Immunology 2020Quote: ... Fc blocking and fluorescent staining was performed as previously described and samples sorted into pure cell populations based on cell surface marker expression using a FACS Fusion (BD Biosciences). For data where peritoneal populations are expressed as cells/ μl ...
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bioRxiv - Immunology 2021Quote: ... or B-2 B cells (B220high CD19+ CD11b-PI-) were sorted into 500ul RNeasy lysis buffer using a FACS ARIA II cell sorter (BD Biosciences). RNA was purified using the RNeasy mini kit (cat# 74134 ...
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bioRxiv - Immunology 2019Quote: ... cDNA was amplified - 10 cycles for resting cells and 9 cycles for in vitro activated cells - using the Human Immune Response primer panel (BD Biosciences), containing 399 primer pairs ...
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bioRxiv - Neuroscience 2021Quote: ... hPDL and hDP derived from different subjects were pooled together to obtain single cell suspensions by passing the cells through a 70 μm strainer (BD Falcon). Cells were centrifuged ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice in wash buffer and residual red blood cells were lysed using BD FACS Lysing Solution (BD Biosciences) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... the endothelial cell monolayers in 6-well plates were lysed after removing center region using cell scraper (BD Falcon #35-3085) and washing with 1X HBSS (Corning #21-022-CV).
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bioRxiv - Cell Biology 2022Quote: ... and GFP-positive single cells were sorted by FACS into 96-well plates using a FACSAria III cell sorter (BD Biosciences) to obtain single cell-derived clones ...
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bioRxiv - Cell Biology 2022Quote: ... Reaction was stopped by adding the wash buffer to the lysed cells and suspensions were filtered through a 40um cell strainer (BD Biosciences). Nuclei concentration was determined by DAPI and Ethidium Homodimer-1 dyes using a Countess II FL Automated Cell Counter ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary AML cells were harvested and stained with human CD45 and cells were analyzed by a high-throughput automated plate reader (BD LSRFortessa). Dead cells were excluded using the Near-IR Live/Dead marker (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: The hybridoma cell lines secreting SOSV-reactive antibodies were cloned using single-cell sorting on a BD FACSAria III cytometer (BD Biosciences) or SH800 Cell Sorter (Sony Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils (CD45+Ly6ghighLy6clowCD11b+CD3-CD19-F4/80- CD11c-) cells and monocytes (CD45+Ly6chighCD11b+Ly6g-CD3-CD19-F4/80-CD11c-) cells were sorted on a FACSAria Instrument (BD Biosciences) and processed for RNA isolation using the RNeasy Plus Micro Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... Mouse CD19 was used as a marker gene to enrich for successfully transduced cells by adding antibodies directed against mouse CD19 and enriching for stained cells by MACS (Miltenyi) followed by FACS on an Aria III cell sorter (BD Biosciences) (see Supplementary file 4 for antibody details) ...
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bioRxiv - Immunology 2022Quote: To analyze the major thymic stromal cell types in human samples single cells suspensions non-specific antibody binding was blocked with human Fc-Block (BD Bioscience) and the cells were subsequently stained with CD45-PE ...
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bioRxiv - Immunology 2022Quote: ... For quantification of the different developmental steps in T cell maturation the cells were blocked with murine Fc-block (BD Biosciences) and stained with either CD45-APC/Cy7 (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: Naive and effector/memory CD4+ T cells were isolated by Fluorescence-activated Cell Sorting (FACS) upon staining with anti-CD4 antibody (BD 562970), anti-CCR7 antibody (353227 BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45highCD8+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45highCD8+ T cells per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Genomics 2022Quote: ... Polyclonal populations of mCherry-positive cells were selected using two rounds of FACS on a FACSAria II Cell Sorter (BD Biosciences). To generate K562 cells stably expressing Zim3-dCas9 without a fluorescent marker ...
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bioRxiv - Microbiology 2024Quote: ... Lymphocyte suspensions were prepared from mesenteric lymph nodes (MLN) by pressing the cells through a 70-μM nylon cell strainer (Falcon; BD Labware) in complete Dulbecco’s modifed Eagle’s medium (cDMEM ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were centrifuged and washed with PBS three times before analysis using a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences).
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: The distribution of cell populations throughout the cell cycle and the fraction of cells capable of incorporating BrdU were determined using the BrdU Flow Kit (BD Biosciences). After treatments cells were incubated with BrdU (10 ⎧M ...
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bioRxiv - Neuroscience 2022Quote: ... neurons or endothelial cells from the HC by flow cytometry a fluorescence-activated cell sorting device type Aria II (BD Biosciences) was used ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4 T cells (live CD19-CD4+CD25-CD45RBhi cells) and colonic monocytes (live CD45+CD19-CD4-CD11b+ cells) were sorted on a FACSAria III (BD biosciences) cell sorter.
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bioRxiv - Bioengineering 2022Quote: ... The specificity of CD8+ T cells towards the peptide was previously assessed by co-culture of 5·104 T cells with pulsed tumor cell lines in the presence of Golgi Plug (BD Biosciences) in 96-well plate at 1:1 E:T cell ratio.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were seeded in a 6.5mm transwell insert at ~20K cells in 200uL of a 50% Matrigel suspension (BD Biosciences, 354230) per transwell ...
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bioRxiv - Cell Biology 2022Quote: ... islets were dispersed to single cells (as per mt-Keima FACS protocol) and sorted on a FACSAria III cell sorted (BD Biosciences). Single cells were gated using forward scatter and side scatter (FSC and SSC ...
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bioRxiv - Molecular Biology 2024Quote: ... and also to eliminate the presence of mitomycin-C-treated MEFs (used as feeders during our mESC cultures) we performed FACS (Fluorescence-Activated Cell Sorting) using a FACSAria cell sorter (BD Biosciences). The dissociated mESC were first labeled with the eBioscienceTM Fixable Viability Dye eFluorTM 780 (ThermoFisher ...