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Citations for Becton, Dickinson and Company :
2101 - 2150 of 10000+ citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... Cells were washed 2x with Permwash buffer (BD) and stained intracellularly for 30 min at 4C with the following markers (SI Appendix) ...
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bioRxiv - Immunology 2024Quote: ... HAP1 SPPL3-/- cells with VPD450 membrane dye (BD) and HAP1 SPPL3-/-B3GNT5-/- with CFSE according to manufacturer’s protocol while the B3GNT5-/- remained unlabelled ...
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bioRxiv - Microbiology 2020Quote: ... These cells were incubated for 30 min at room temperature and then placed on ice for a further 30 min prior to analysis on a Becton Dickinson FACSCalibur™ system (BD Biosciences) using a FL2-height detector ...
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bioRxiv - Microbiology 2019Quote: ... The cell pellet was then resuspended in 1 ml of T-R minimal medium and injected into the IsoCaRB system using a 3 ml syringe (BD Biosciences # 309657) and a 20-gauge needle (Sigma-Aldrich #Z192511).
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bioRxiv - Immunology 2021Quote: ... The purity and readiness of immature DCs was assessed by flow cytometry on a FACSCanto II (BD Immunocytometry Systems, San Jose, CA). The DCs were phenotyped using phycoerythrin-conjugated (PE ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies (Claudin-5, Abcam Ab131259, 1:1000; Collagen IV, Abcam Ab6586, 1:500; Hemoglobin, R&D Systems G-134-C, 1:300; P62, BD Biosciences 610833 ...
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bioRxiv - Microbiology 2021Quote: ... The cMRS agar plates were incubated at 30 °C under aerobic or anaerobic conditions (BD BBL GasPak system (BD, Franklin Lakes, NJ) for 48 h ...
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bioRxiv - Molecular Biology 2021Quote: ... A second sample (ca. 600 μL) was collected by cardiac puncture under isoflurane anaesthesia into a Li-Hep microtainer (BD Diagnostic Systems). Following collection ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Developmental Biology 2023Quote: ... Single cells were captured and libraries prepared using the BD Rhapsody system with the BD Rhapsody cDNA Kit (Cat. No. 633773) and Whole Transcriptome Analysis (WTA) Amplification Kit (BD Biosciences, 633801). The WTA and Sample Tag libraries were amplified and purified according to the manufacturer’s protocol (https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/BD_Rhapsody_System_mRNA_Whole_Transcriptome_Analysis_and_Sample_Tag_Library_Preparation_Protocol.pdf) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein blots were visualized using Western ECL Substrate Kit (Thermo Pierce) and exposed using a ChemiDoc XRS+ system (BD, Franklin Lakes, NJ). The intensity of bands was quantified by Image J software
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bioRxiv - Neuroscience 2024Quote: ... a 10 cc mL blood sample was collected per session by a licensed phlebotomist via the insertion of a saline-lock intravenous line into the dominant or non-dominant forearm and the use of a vacutainer SST (BD Diagnostic Systems). For both participants ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with sort buffer and incubated with cell surface antibodies mix diluted in the BV stain buffer (BD Biosciences) following manufacturer instructions for 30 minutes on ice in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... The CSCs were selected as the top 5% cells with the highest GFP intensity (Oct4hi) and then sorted with the FACSAria II cell sorter (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... AIM assay was conducted as previously explained and CD69+CD40L+CD4+ T cells were live-sorted on a FACS Aria cell sorter (BD BioSciences) equipped for handling of biohazardous material ...
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bioRxiv - Genomics 2020Quote: ... Bacterial cells were examined by size (FSC-A) and granularity (SSC-A) using Fluorescence-activated cell sorting (FACS, BD FACS Melody), and cells were then sorted into 96-well microplates with 100-cell and single-cell samples were included in our experiments ...
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bioRxiv - Neuroscience 2021Quote: ... whereas unsorted endothelial and perivascular cells were obtained from the SYTOXTM blue- fraction Cells were sorted by a FACS Aria III (BD Bioscience) sorter using the four-way purity sorting mode directly in EGM2 medium (#CC-3162 ...
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bioRxiv - Cell Biology 2022Quote: ... Multicolor flow cytometry and cell sorting were performed with an LSR Fortessa or BD FACSAria cell sorter using BD FACSDIVA software (BD Biosciences). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Homogenate was passed through 40-μm nylon filter to obtain a single-cell suspension and subjected to red blood cell lysis reagent (BD Pharm Lyse, BD Biosciences). Cells were subjected to negative selection using CD45 magnetic microbeads (Miltenyi Biotec # 130-052-301) ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton-X (proliferating abl pre-B cells) or 0.5% Triton-X (MCF10A cells) in PBS and fixed with BD Cytofix/Cytoperm solution (BD Biosciences, #554722) containing 4.2% formaldehyde ...
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bioRxiv - Developmental Biology 2019Quote: ... the cell suspensions were then filtered through a 40 μm nylon cell strainer (BD Falcon, BD Biosciences, San Jose, CA, USA) prior to single cell library construction.
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bioRxiv - Cancer Biology 2019Quote: ... and then cells (expressing red fluorescence) were subjected to a second selection by FACS on a FACS/Aria Fusion Cell Sorter (BD Biosciences). Stable transfectants were maintained in complete medium plus 1.0 μg/ml puromycin.
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bioRxiv - Bioengineering 2019Quote: ... the cell culture mixture was stained with an antibody recognizing the neural cell adhesion molecule (NCAM/CD56; BD Pharmingen; Table S1), and the myogenic progenitor (CD56+ ...
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bioRxiv - Developmental Biology 2019Quote: ... Dissociated cells were then filtered through a falcon tube with a cell strainer cap (BD Biosciences; BD falcon 12 × 75 mm) in which the filter was previously dampened with 500 µl of incubation media ...
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bioRxiv - Developmental Biology 2019Quote: ... Dissociated cells were then filtered through a falcon tube with a cell strainer cap (BD Biosciences; BD falcon 12 × 75 mm) in which the filter was previously dampened with 500 µl of incubation media ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in ice cold PBS and supplied with 10 μl of 7-AAD to assess cell viability (BD Pharmigen) and sorted with a BD Aria III FACS as previously described 30 ...
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bioRxiv - Immunology 2019Quote: ... were sorted directly into U-bottom 96-well plates containing cell culture media using an Aria III cell sorter (BD Biosciences). For each cell type we sorted 4 single cells ...
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bioRxiv - Cancer Biology 2019Quote: SUIT2 cells (4 × 106) and A549 cells (3 × 106) were dispersed in 75 μL DMEM and 75 μL Matrigel (356230, BD Biosciences) and injected subcutaneously into nude mice ...
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bioRxiv - Immunology 2019Quote: ... The cells were then aliquoted into FACS tubes (0.25-1×106 cells/tube) with strainer caps (BD Biosciences, San Jose, CA).
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bioRxiv - Genomics 2020Quote: ... Approximately 10,000 LK (Lin-, CD117+) cells per sample were sorted using the BD FACSMelody cell sorter (BD Biosciences, San Jose, California) into 1 x PBS containing 5% BSA.
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bioRxiv - Immunology 2019Quote: ... Memory CD4 T cell sorting (see gating strategy Figure supplement 1A) was performed on a BD Aria III/Fusion cell sorter (BD Biosciences). 100,000 memory CD4+ T cells were sorted into TRIzol LS reagent (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Single cells from specific populations were collected for subsequent bimolecular analysis by FACS-sorting using a FACSAria II cell sorter (BD Biosystems), SONY SH800 ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP positive cell populations of both doxycycline treated and untreated populations were sorted on a FACSAria II cell sorter (BD Biosciences) and flow cytometry data analyzed with FlowJo software ...
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bioRxiv - Bioengineering 2021Quote: ... H-2Kb-negative GFP-positive cells were sorted into single cells on a 96-well plate using FACSAria Fusion (BD Biosciences) and cultured for 2-3 weeks in D10 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were then suspended in alginate precursor solution ‘A’ at 5 x 106 cells/mL (final concentration 2.5 x 106 cells/mL) and loaded into 1 or 3 mL syringes (BD luer lock). Following previous protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... 48 h after transduction the cells were sorted positively by fluorescence activated cell sorting (FACS) using BD FACS Aria flow cytometer (BD Biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... xenograft models were generated by injecting ES2-shARID1A cells [2 × 106 cells mixed 1:1 (v/v) with Matrigel (BD Biosciences)] subcutaneously ...
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bioRxiv - Cell Biology 2021Quote: ... The cultures were analyzed for BFP/GFP loss by flow cytometry followed by fluorescence-activated cell sorting (FACS) using a cell sorter (FACSAria III, BD Biosciences) at a rate of 10,000 events/s ...
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bioRxiv - Cell Biology 2021Quote: ... Stained cells were diluted to the desired cell density in 1x PBS and analyzed (≥30, 000 cells) by flow cytometry (FACSAria III, BD Biosciences) at a rate of 250-1000 events/s ...
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bioRxiv - Cancer Biology 2020Quote: ... Fluorescence activated cell sorting (FACS) of ALDEFLUOR-treated cells was performed on an Aria II sorter using FACS DiVa software (BD Biosciences). The brightest 2–5% of ALDEFLUOR-treated cells were identified and gated electronically based on their characteristic light-scatter properties on the fluorescein isothiocyanate (FITC)-channel emission pattern after excitation with a 13–20 mW ...
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bioRxiv - Cancer Biology 2019Quote: ... After a further 48 hours cells were harvested and the number of GFP-positive cells was measured by flow cytometry (Fortessa-BD Biosciences). For complementation experiments ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were implanted with 5 x 104 MDA-MB-231 breast cancer cells in 50% cell culture medium (FBS free) and 50% Matrigel (BD biosciences) by subcutaneous injection in the left dorsal flank ...
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bioRxiv - Cell Biology 2021Quote: ... The percentage of GFP-positive cells among the mCherry-positive cells was determined 48 h after I-SceI transfection using a LSRFortessa X-20 (BD Bioscience) using BD FACSDiva Software v8.0.1 ...
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bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 x g cells were carefully resuspended in cell culture medium with 10 µl/ml 7-AAD (BD Biosciences). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Cells were aliquoted to a count of 1 million cells each and incubated on ice with Fc Block (BD, Table S1) at a 1:20 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Selection of the positive cells was performed using 4 µg/ml puromycin and viable cells were expanded for flow cytometry cell sorting using a FACSMelody (BD Biosciences) equipped with a 561 nm excitation laser and 613/18 filter ...
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bioRxiv - Microbiology 2021Quote: ... GFP-positive transduced CHOK1 cells expressing RBD-mFc were sorted by flow cytometry using a BD FACSAria III cell sorter (BD Biosciences) and suspension adapted in PROCHO5 medium (Lonza ...
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bioRxiv - Molecular Biology 2021Quote: ... between 500.000 and 1 million GFP-mCherry double positive cells were sorted each day for 5 consecutive days using two cell sorters (BD FACSAriaII SORP and BD FACSAriaIII). Sorted cells were pooled ...
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bioRxiv - Cancer Biology 2020Quote: ... For both ACHN and CAKI-1 cell lines 2×106 cells were diluted in 50 µl of PBS and 50 µl of Matrigel (BD Biosciences) and were injected subcutaneously into the right and left flank of each mouse ...
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bioRxiv - Genomics 2021Quote: ... and 48h after induction of 2i-release the cells been dissociated into single cells using accutase before flow sorting (BD Influx) into 96-well plate containing 2.5μl of methylase reaction buffer (1 × M.CviPI Reaction buffer (NEB) ...