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Citations for Becton, Dickinson and Company :
2351 - 2400 of 10000+ citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Human cytokines expression (hTNFα and hIFNγ) in human T cells were analyzed using BD LSRFortessa™ cell analyzer - flow cytometer (BD Biosciences) and the data were analyzed using FlowJo software (Dako).
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bioRxiv - Immunology 2020Quote: ... from paired BAL fluid and PBMCs were either bulk sorted in 100-cell mini-populations or single-cell sorted using the FACSAria™ III (BD Biosciences) in purity mode into 96-well microplates containing 10 μL of 1% 2-mercaptoethanol RLT buffer (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... cells were analysed using fluorescence-activated cell sorting (FACS) and sorted at 20 cells per well in 96-well plates using a BD FACSAria™ III (BD Biosciences). Two cell phenotypes were sorted ...
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bioRxiv - Microbiology 2020Quote: ... EBV-infected Akata cells that express EGFP were sorted by a FACS Aria II Cell Sorter (BD Biosciences, San Jose, CA, USA) at 2 dpi and then cultured ...
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bioRxiv - Cancer Biology 2020Quote: ... two groups of NOD/SCID female mice were orthotopically injected in the mammary fat pad #4 with 2×106 cells of either ALDH1A3 overexpressing or vector control MDA-MB-231 cells (admixed with high concentration Matrigel, BD, Cat # 354262). Mice were euthanized 49 days post cancer cell implantation and the lungs were harvested ...
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bioRxiv - Genetics 2020Quote: ... 0.5 × 106 DAPI-/SSEA4+/TRA-1-60+ cells were collected for each cell line using the BD FACSAria™ II flow cytometer (BD Biosciences). Collected cells were pooled and plated at a density of 30 000 /cm2.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... a yeast IgG library was subjected to multiple rounds of selection by magnetic activated cell sorting (MACS) and florescence activated cell sorting (FACS, BD ARIA III) in PBS buffer containing 1mM MnCl2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were scraped using a scraper and cell suspension was analyzed for nanoparticles uptake study using Flow Cytometer (BD FACS Calibur, USA).
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bioRxiv - Neuroscience 2021Quote: ... A 1ml astrocyte cell suspension of 4×104 cells/ml was plated in three chambers of four-chamber glass slides (BD Falcon, USA) coated with poly-D-lysine ...
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bioRxiv - Cell Biology 2021Quote: ... The ZsGreen-ODC (ornithine decarboxylase) cells were previously generated by transfection of MelJuSo cells with the ZsProsensor-1 plasmid (BD Bioscience Clontech)20 ...
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bioRxiv - Biophysics 2020Quote: ... Stable mNeonGreen-mGluR expressing HEK293T cell lines were produced by transducing cells with either pCDH-puro-mNG-mGluR3 or pCDH-puro-mNG-mGluR5 and enriched by fluorescence-activated cell sorting (BD Biosciences FACSAria).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were analyzed for GFP+ frequencies by flow cytometry as for the other reporter assays and/or used for cell sorting of GFP-negative (GFP-neg) cells (BD Aria sorter).
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bioRxiv - Immunology 2021Quote: ... For cell staining cells were first blocked with 1 μg/ml Fc-Block (mouse Fc Block, BD Biosciences, San Jose, CA, USA) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 30 min with the following cell surface antibodies: BUV661-anti-CD45 (D058-128, BD Biosciences 741657 Lot 0198214), PE-Cy7-anti-CD20 (2H7 ...
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bioRxiv - Immunology 2022Quote: ... single-cell suspensions were subjected to osmotic red blood cell lysis and incubated with a combination of antibodies: CD4-BV421 (BD Biosciences #562891), CD8-PeCy7 (BD Biosciences #561097) ...
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bioRxiv - Genetics 2022Quote: K562 cells were electroporated using sgRNA guide targeting ASXL1 or SRSF2 genes followed by sorting for live single-cells using BD FACSMelody™ Cell Sorter (BD Biosciences). Sorted K562 cells were plated onto 96-well plates containing 100ul/well RPMI 1640 Medium with L-Glutamine (Biological Industries ...
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bioRxiv - Microbiology 2024Quote: ... Cells (1x106 cells/mL) were cultured in a 48-well plate pre-coated with anti-CD3 monoclonal antibody (BD Pharmingen, 5μg/mL), and culture supernatants were collected after 72h of stimulation and stored at -80°C until assayed for cytokine production ...
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bioRxiv - Cancer Biology 2023Quote: Flow-cytometric analysis of mouse bone marrow cells or MUTZ3 cells was done with specific antibody stainings using mouse CD11B-APC (BD, cat 553311) and mouse Gr-1 FITC (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: For single-cell RNA sequencing experiments cells were washed and stained with anti-CD45-PE/Cy7 (BD Pharmingen, clone 30-F11, #552848) and SYTOX™ Green Ready Flow™ Reagent (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... The FCS files obtained for DNA content profiling were analysed for the proportion of cells in each cell cycle stage using the Watson Pragmatic model59 in FlowJoTM Software (BD Life Sciences).
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bioRxiv - Immunology 2023Quote: ... T cells and RP KD tumor cells were then co-cultured for 6 hours in the presence of GolgiPlug (BD Biosciences 555029) and GolgiStop (BD Biosciences 554724 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells with green fluorescence or blue fluorescence were harvested using a BD FACSAria™ II Cell Sorter (BD Biosciences, Bedford, MA, USA) for the following experiments.
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bioRxiv - Cell Biology 2023Quote: ... T cells with residual target protein expression were depleted by antibody staining and bead pull-down or cell sorting (BD Aria III). T cells were re-suspended in MACS buffer (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... around 2×105 HEK001 cells expressed GFP (GFP+ HEK001) isolated by FACS Aria II cell sorter (BD Biosciences, San Jose, CA, USA) and cultured in one well of a 24-well plate ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice with 1x Perm/Wash buffer and measurements were acquired on cell analyzer FACS Lyrics (BD Bioscience, Heidelberg, Germany). Controls without PMA and Ionomycin stimulation were included in this assay ...
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bioRxiv - Cancer Biology 2022Quote: ... about 1,000 endometrial epithelial cells or endometrial stromal cells (those desired for each condition) were resuspended in 10 μl of Matrigel (BD Biosciences, 354234) per well of M96 ...
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bioRxiv - Immunology 2023Quote: ... The lymphocyte fractions were stained with anti-CD45-APC (panhematopoietic cell marker) and propidium iodide (Live/Dead stain) before sorting on a FACSAria Fusion cell sorter (BD Biosciences, USA). Live intrahepatic immune cells (CD45-FITC-CD45-APC+ PI- ...
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bioRxiv - Cancer Biology 2023Quote: The cell cycle distribution was detected immediately by flow cytometry using the BD LSRFortessa Cell Analyzer (BD Biosciences, San Jose, CA, USA). Data were collected using BD FACSDiva Software (v8.0.1 ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were harvested to assess cell health and apoptotic status using the PE Annexin V Apoptosis Detection Kit I (BD, Cat. # 559763) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the ratios of GFP+ cells to mCherry+ cells were measured by a LSR Fortessa flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
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bioRxiv - Microbiology 2023Quote: ... PI-negative cells were gated and sorted with index sorting (single cell precision mode activated from sorting layout in the BD FACSDiva software) onto Mueller Hinton agar (MHA ...
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bioRxiv - Bioengineering 2023Quote: ... a correlation analysis of both forward (indicates cell size) and side scatter (indicates cell granularity) was performed by flowcytometry (BD LSR-Fortessa) capturing a minimum of 5,000 cellular events in a forward-side scatter gate based on cell size and granularity ...
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bioRxiv - Synthetic Biology 2023Quote: ... G1ER cells were sorted for GFP expression (NanoLuc biosensors) or CyOFP1 expression (BRET biosensors) with flow cytometry (BD FACSAria II Cell Sorter). Sorted cells were checked for biosensor expression by surface staining with Myc-Tag antibody (Myc-Tag 9B11 Mouse mAb ...
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bioRxiv - Cancer Biology 2023Quote: ... A pure polyclonal population of dCas9-KRAB expressing cells was generated by two rounds of fluorescence activated cell sorting (FACS) gated on the top half of BFP+ cells (BD FACS Aria). The performance of MCF7 CRISPRi in knocking down endogenous genes was confirmed by individually targeting 3 control genes (ST3GAL4 ...
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bioRxiv - Microbiology 2024Quote: ... Stained memory B cells (MBCs; CD3/CD14/CD56- CD19+ CD27+ IgD- IgM-) were single-cell sorted with a BD FACSAria™ Fusion (BD Biosciences) into 384-well microplates containing 3T3-CD40L feeder cells and were incubated with IL-2 and IL-21 for 14 days71.
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bioRxiv - Neuroscience 2023Quote: ... The cells were then stained at a 1:50 dilution with antibodies to immune cell targets as follows: CD3- FITC (BD Biosciences, 555332), CD8-PE (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... The media containing dye was removed and the cells were washed with 1XPBS and the cells were acquired immediately for flow-cytometry (BD FACS Verse) (Ex-491nm/Em-513nm).
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bioRxiv - Genetics 2024Quote: ... After 24 hours we selected the transfected cells either by puromycin treatment (2 ug/ml for 2 days) or flowcytometric sorting for double positive cells (BD FACSAriaTM fusion).
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bioRxiv - Immunology 2024Quote: ... cells were washed and resuspended in FACS staining buffer for analysis on a BD FACSCelesta Cell Analyzer (BD Biosciences, San Jose, CA). Flow cytometry data was analyzed using FlowJo v10 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were resuspended with DAPI and annexin V/DAPI negative cells were sorted for subsequent ADT staining and single cell capture per established protocols (BD Rhapsody™). The BD Rhapsody™ workflow is a nanowell based single cell capture system with established protocols for generating single cell whole transcription and ADT cDNA libraries ...
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bioRxiv - Bioengineering 2024Quote: ... DAPI dye was added to stain for live/dead and cells were gaited on DAPI and GFP with untransfected cells used as a negative control for background (BD FACSAria Fusion).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were detected using flow cytometry (FACS Calibur, BD) and quantitative analysis was carried out using FlowJo software (RRID:SCR_008520).
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bioRxiv - Cancer Biology 2021Quote: Cell apoptosis was quantified using flow cytometry (FACSCalibur, BD) as described previously (Elkabets et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were analyzed on LSRFortessa X-20 (BD Biosciences) with appropiate fluorescence compensation ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were suspended in Matrigel (BD Worldwide, Cat#354234) at a concentration of 2×104 cells per microliter and placed on ice ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated with Golgistop (554724, BD Biosciences) (dilution 1:1500 ...
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bioRxiv - Immunology 2021Quote: Isolated cells were stained with Fc block (BD Sciences) for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... cells were sorted using BD FACS AriaIllu (BD Biosciences) into hematopoietic stem cells (HSCs) ...
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bioRxiv - Immunology 2021Quote: ... cells were first treated with cytofix/cytoperm (BD Biosciences) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... All cells were fixed using Fix/Perm (BD Biosciences) for 20 min prior to intracellular staining and/or analysis ...