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Citations for Becton, Dickinson and Company :
2201 - 2250 of 7462 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... After 6 or 7 days cells were resuspended in enzyme-free EDTA buffer and replated in untreated 6-well plates (BD Falcon, Sigma-Aldrich) at 1 M cells per well in unconditioned medium with or without priming in 25 U IFN-γ (PeproTech ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... α7 integrin-PE antibody (AbLab, Vancouver, Canada), CD9-APC antibody (eBioscience, San Diego, CA) and CD47-BV605 antibody (BD Biosciences, San Jose, CA) for 30 minutes on ice and washed ...
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bioRxiv - Cancer Biology 2019Quote: ... For apoptosis analysis was performed by a double staining Annexin V and 7-AAD Apoptosis Detection Kit (BD Pharmingen, San Jose, CA, USA) following the manufacture’s introductions ...
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bioRxiv - Immunology 2020Quote: ... 7 days post-vaccination) and measured Cytokines using the BD CBA Mouse Th1/Th2/Th17 Cytokine Kit (BD Bioscience, San Jose, CA, USA). Sera samples were processed as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cell pellets from the single-cell suspension were resuspended in FACS buffer (PBS, 5% FBS, and 5 mM EDTA) for staining in ice for 10 min with 7-AAD (BD Biosciences, #51-68981E). Only 7-AAD- cells were considered as viable cells ...
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bioRxiv - Immunology 2021Quote: ... One million cells per sample were pre-incubated with purified anti-mouse CD16/CD32 antibody (Fc Block™, 553141, BD Biosciences). Live/dead cells were discriminated by staining cells with the LIVE/DEAD® Fixable Near-IR Dead Cell Stain kit (L10119 ...
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bioRxiv - Immunology 2021Quote: ... 2000 cells of the CD45−CD31+CD146+ cell population were sorted into eppendorfs containing 125 μL PBS on one of the two available FACSAria™ II or III machines (BD). After sorting ...
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bioRxiv - Cell Biology 2022Quote: ... were transformed with one plasmid derived from pPC86 (GAL4-activation domain, AD (‘Prey’)) and pPC97 (GAL4-DNA-binding domain, BD (‘Bait’)) (Chevray and Nathans ...
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bioRxiv - Immunology 2021Quote: ... lymphocytes were stained for one hour at room temperature in the dark with CD56-APC (Miltenyi) and CXCR4-PE (BD Biosciences) or CD3 APC-cy7 (Sony ...
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bioRxiv - Immunology 2022Quote: ... 0.75 × 105 naïve T cells were cultured for one or two days with plate-bound anti-CD3ε (2 µg/ml, clone 145-2C11; BD Biosciences) in differentiation medium consisting of RPMI1640 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2019Quote: Three thousand flow sorted DAPI-CD45-CD31-Epcam+GFP+ cells were cultured with one hundred thousand MLg mouse lung fibroblasts and Matrigel (BD Biosciences) seeded onto Transwell filter inserts (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... one million cells were resuspended in FACS buffer and stained with Cd11b PE-Cy7 (BD 552850, clone M1/70, lot 0058856) and GR-1 (Ly-6G and Ly-6C ...
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bioRxiv - Genomics 2020Quote: Immune cells for bulk mRNA sequencing were incubated with Fc Block for 20 minutes and then stained with one of six panels of directly conjugated antibodies for 30 minutes: anti-human CD16 (BD 558122), CD123 (BD 560826) ...
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bioRxiv - Physiology 2022Quote: ... Cells were loaded into one BD Rhapsody™ Cartridge which was primed and treated strictly following the manufacturers protocol (BD Biosciences). Cell Capture Beads (BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Thermo Fischer) for one hour at room temperature and co-stained with anti-γH2AX coupled to Alexa 647 (560447, BD Biosciences) and anit-mCherry coupled to Alexa 594 (M11240 ...
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bioRxiv - Molecular Biology 2022Quote: ... blocked with Sea-block buffer (37527- Thermo Fischer) for one hour at room temperature and co-stained with Alexa 647-coupled mouse anti-γH2AX (560447, BD Biosciences) and FITC coupled goat anti-GFP (NB100-1771 ...
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bioRxiv - Genomics 2024Quote: ... Three to five 96-well plates were seeded with one fluorescent cell per well using the BD FACSAriaTM II (BD Biosciences), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 48h post transfection cells were sorted for single cell clones by seeding one RFP- and GFP-positive cell per 96-well using a FACS Melody sorter (BD Bioscience). The deletion of IGF2BP1 was validated by western blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... We sorted single nuclei in 96-or 384-well plates (one nucleus per well in 10 nL sorting volume) using the BD FACSJazz Cell Sorter (BD Biosciences) based on forward and side scatter properties ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining for IFN-γ and granzyme B was conducted in Perm/Wash buffer after fixation and permeabilization in Fix/Perm buffer (BD Biosciences, USA) according to kit instructions ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 4°C for 30 min with a B cell surface marker antibody cocktail (Table S11) with 10 µl Brilliant Stain Buffer Plus (BD, cat. No. 566385) diluted in PBS containing 1% BSA up to a volume of 100 µl ...
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bioRxiv - Genomics 2020Quote: ... at a concentration of 100μg/ml starting 5 days after transfection for the following 7-9 days before single cell sorted using BD FACSAria II (BD Bioscience, San Jose, CA) for clonal cell line generation ...
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bioRxiv - Cell Biology 2019Quote: ... were sorted by FACS and plated at one cell per well in a 96-well plate using a BD InfluxTM cell sorter (BD Bioscience, USA). About 10-12 days into the culture ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells from the remaining human lung donors were divided in half and labelled with one of each of the following antibody cocktails before sorting using a FACSMelody instrument (BD Biosciences, Germany). Antibody mix 1 (endothelial/epithelial) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cell suspensions were divided in half and labelled with one of each of the following antibody cocktails before sorting using a FACSMelody instrument (BD Biosciences, Germany). Antibody mix 1 (endothelial/epithelial/leucocyte) ...
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bioRxiv - Immunology 2019Quote: ... 1 µL of mAbs were also added to one drop of compensation beads (Anti-Rat Ig, κ/Negative Control Compensation Particles Set (BD Biosciences)) to prepare single-stain controls for compensation ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then blocked with 10% goat serum in staining buffer (1% bovine serum albumin (BSA) plus 0.05% Tween 20 in PBS) for one hour prior to staining with mouse anti-GM130 (BD Transduction Laboratories 610822) or mouse anti-Calreticulin (abcam ab22683 ...
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bioRxiv - Genomics 2022Quote: ... Emerging cells were induced for 24 h with 2.5 µg/ml doxycycline and then subjected to Fluorescent-Activated Cell Sorting (FACS) to select the ones expressing the mVenus (BD FACSAria™ II, BD Biosciences). H3 and H4 overexpression was verified by Western Blot with anti-H3 and anti-H4 antibodies (Abcam ...
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bioRxiv - Genomics 2020Quote: ... Treg cells (CD3+, CD4+, CD8-, CD25+), B cells (CD3-, CD19+), and monocytes (CD3-, CD14+) were sorted by FACSAria III (BD Pharmingen, San Diego, USA) directly into wash buffer for CUT&Tag ...
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bioRxiv - Immunology 2019Quote: ... Viable antigen-specific B cells (AqVd-CD14-CD16-CD3-CD19+ IgD-) were single-cell sorted with a BD FACSAria II-SORP (BD Biosciences, Mountain View, CA) into 96 well PCR plates and stored at −80°C for RT-PCR.
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bioRxiv - Cancer Biology 2022Quote: One hundred thousand human ALL cells were seeded in a volume of 100 μL B cell medium/well (as described above) on Optilux 96-well plate (BD Biosciences, San Jose, CA). EHT1610 ...
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bioRxiv - Neuroscience 2020Quote: ... Explants were grown on 12-mm diameter glass coverslips coated with 20 μg/mL laminin for 2D cultures used for time-lapse imaging or coated with 7 μl of Matrigel™ (BD Biosciences, Mississauga, ON, Canada) for 3D cultures used for morphological analysis ...
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bioRxiv - Immunology 2021Quote: ... and intracellular cytokines (PE anti-mouse IFN-γ, PE-Cy®7 anti-mouse TNF-α, APC anti-mouse IL-2, all from BD Biosciences, USA). The labeled cells were acquired with the BD FACSCanto™ II flow cytometer and analyzed with the program FlowJo v.10.6.2 (BD Biosciences).
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bioRxiv - Cancer Biology 2021Quote: ... mice received suspension of 0.2 mg FPM in a total volume of 50 μl of sterile physiological saline by inserting a 7-gauge needle (BD Biosciences, San Jose, CA, USA) into the trachea trans-orally ...
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bioRxiv - Cell Biology 2022Quote: ... Sorting gates were set according to the corresponding fluorescence-minus-one (FMO) controls and cells were sorted on a FACS Aria II or Aria III (BD Bioscience, Erembodegem, Belgium). Dead cells were excluded by 7-Amino-actinomycin (7-AAD ...
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bioRxiv - Microbiology 2019Quote: ... washed one time with PBS and resuspended in PBS for analysis on a LSR Fortessa flow cytometer (BD Biosciences, San Jose, CA, USA) with BD FACSDiVa™ Software (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... the individual antibodies used for staining were mixed with one drop of negative control beads and anti-mouse IgG beads (BD Biosciences, CA, USA). For the dead cell stain ...
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bioRxiv - Cancer Biology 2020Quote: ... enrichment of leukemic cells was carried out by depleting non-B-cells using streptavidin-beads (BD Streptavidin Particles Plus, BD Biosciences, Franklin Lakes, NJ, USA) and biotinylated antibodies against human CD16 (clone 3G8) ...
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bioRxiv - Cell Biology 2019Quote: ... 3-6 draws per donor (with each set of experiments performed on one draw from each donor) were collected into K2EDTA-coated BD Vacutainer® Blood Collection Tubes (Becton, Dickinson and Company), transferred to polypropylene tubes (Corning) ...
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bioRxiv - Immunology 2021Quote: ... Appropriate compensation and fluorescence-minus-one (FMO) controls were generated in parallel either with aliquots of bone marrow cells or Rainbow Fluorescent Particle beads (BD Biosciences; Cat. No. 556291). All antibody concentrations were titrated prior to injury study using primary bone marrow cells to ensure optimal separation and detection of true negative and positive populations ...
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bioRxiv - Physiology 2020Quote: ... Appropriate compensation and fluorescence-minus-one (FMO) controls were generated in parallel either with aliquots of bone marrow cells or Rainbow Fluorescent Particle beads (BD Biosciences; Cat. No. 556291). All antibody concentrations were titrated prior to injury study using primary bone marrow cells to ensure optimal separation and detection of true negative and positive populations ...
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bioRxiv - Immunology 2024Quote: ... blood samples were collected from participants in sodium heparin or lithium heparin Vacutainer CPT Mononuclear Cell Preparation Tubes (BD Biosciences or Greiner Bio-One). PBMC were isolated by density centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... mice were securely hold in one hand by the pelvic girdle and inserting a BD Ultra-Fine® (29G) insulin syringe (BD, Franklin Lakes, NJ, USA) directly on subarachnoid space (close to L4–L5 segments ...
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bioRxiv - Cell Biology 2022Quote: ... 12 mL of blood was collected (one drawing) from each individual into two BD Vacutainer® K3-EDTA-coated collection tubes (BD Biosciences, San Jose, USA) (Figure 10 – step I) ...
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bioRxiv - Genomics 2022Quote: ... Emerging cells were induced for 24 h with 2.5 µg/ml doxycycline and then subjected to Fluorescent-Activated Cell Sorting (FACS) to select the ones expressing the mVenus (BD FACSAria™ II, BD Biosciences). H3 and H4 overexpression was verified by Western Blot with anti-H3 and anti-H4 antibodies (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... and one minute later 100-150 μL of virus injected into the retro-orbital sinus using a U100 insulin syringe (BD micro-fine 0.3mL, 30 gauge needle). The volume was determined based on the animal’s weight15 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse-α-Flotillin-1 (1:1000, clone 18/Flotillin-1; BD Biosciences), mouse-α-LC3 (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... CA or 1: 1000 anti-OPA-1: 612607 and 1: 1000 anti-Drp-1: 611113 obtained from BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... 1:4000 Sca-1-PE-Cy7 (BD Biosciences ...