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Citations for Becton, Dickinson and Company :
2051 - 2100 of 7027 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton-X (proliferating abl pre-B cells) or 0.5% Triton-X (MCF10A cells) in PBS and fixed with BD Cytofix/Cytoperm solution (BD Biosciences, #554722) containing 4.2% formaldehyde ...
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bioRxiv - Immunology 2019Quote: ... IgGpos B cells (100,000 cells from each donor) were sorted on a FACSAria II cell sorter (BD Biosciences, San Jose, CA) and collected for scBCR-seq (Supplementary Fig ...
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bioRxiv - Immunology 2019Quote: ... IgM-negative B cells were purified from lymphocytes using biotinylated anti-rat IgM (Clone G53-238, BD Biosciences, San Jose, CA) and magnetic separation (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Spike-specific memory B cells were stained and single-cell-sorted from PBMCs using a three-laser FACSAria cell sorter (BD Bioscience) by gating on live ...
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bioRxiv - Immunology 2020Quote: ... A maximum of 10,000 live HA-specific mature B cells (CD3-B220+IgD- rHA+) were sorted and collected in a BD FACSAria fusion or BD FACSAria III (BD Biosciences) cell sorter and processed immediately.
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bioRxiv - Immunology 2020Quote: ... naïve splenic B cells were enriched by the depletion of CD43 expressing cell using biotinylated anti-CD43 antibody (Clone S7, BD Biosciences), BD IMag Streptavidin Particles Plus and the IMag® system (BD Biosciences) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We selected cells by their yellow fluorescence intensity according to the selection criteria described in Figures 2A-B with an Aria III cell sorter (BD Biosciences). Specifically ...
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bioRxiv - Immunology 2022Quote: Fentanyl-reactive B cells were analyzed on a LSR II instrument and isolated using an ARIA II cell sorter (BD Bioscience). Cells were stained using fentanyl-PE (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... B and T-lymphocytes and CD11c+ phagocytes were sorted on a FACSAria IIu Flow Cytometry Sorter (BD Biosciences, San Jose, CA) and into 0.22 µm Spin X centrifuge tube filters (Corning ...
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bioRxiv - Immunology 2020Quote: ... 5×106 cells were stained for 30 min at 4°C with the fluorescent probes, a viability marker (LiveDead-eF780, eBiosciences) and the following B cell-specific antibodies: anti-CD20-PE-CF594 (2H7; BD Biosciences), anti-IgG-PE-Cy7 (G18-145;BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... BCR-expressing B cells were FACS sorted on IgG and GFP double-positivity using a FACS Aria-II SORP (BD biosciences). B cells were then expanded and cultured indefinitely.
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bioRxiv - Immunology 2020Quote: ... The RBD+ frequency of sorted B cells was analyzed post-sort using the index file data in Flow Jo version 9.9.4 (Becton, Dickinson and Company).
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bioRxiv - Immunology 2021Quote: For Reverse Transcription PCR demonstrating 3BNC117 gene integration into the IgH locus, RNA was extracted from sorted engineered B cells (3BNC117+, CD4-, CD19+) on a FACS BD AriaIII (BD Biosciences) using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... PD-L1low and non-B cells from the splenocytes population was carried out by cell sorting in a FACSAria II (BD Biosciences) using different gates strategies (see Supplementary Fig.1).
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bioRxiv - Immunology 2020Quote: ... CD138 (plasma cell marker) and other B cell surface molecules on a flow cytometer (BD LSR II™ or Celesta™). To analyzed immune cells in vivo ...
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bioRxiv - Immunology 2022Quote: ... and the antigen-specific B cell population was identified by flow cytometry using a BD ACCURI C6 flow cytometer (BD Biosciences). The non-parametric Kruskal–Wallis test was used to compare the medians (H statistic ...
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bioRxiv - Genomics 2022Quote: ... single GC Light Zone B cells (B220+ GL7+ Fas+ CD86+ CXCR4-) were sorted into in lysis buffer using Aria III flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... Single-cell sorting of 7AAD−PfCSP+CD19+ B cells gated positive for either IgG or CD27 as PfCSP memory B cells and 7AAD−CD38+CD27+CD19+ cells as plasmablasts was performed using a FACS Aria II (BD Biosciences) with FACSDiva software (version 8.0.1 ...
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bioRxiv - Immunology 2023Quote: ... FO B (CD19+B220hiCD43− CD23+CD21lo) cells and ABCs (CD19+B220hiCD43−CD23−CD21loCD11b+CD11c+) were purified by FACS Melody (BD Biosciences) as shown in Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... ProB (34+38+19+) and V.) PreB/Mature B-cells (34-19+) were purified by flow cytometry (FACS Aria, BD-Pharmingen). Data acquisition and analysis were done with CellQuest (Beckon Dickinson ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were discarded and cells resuspended in FACS buffer and tetramer-binding B cells were individually sorted using a 5-laser (355nm, 405nm, 488nm, 561nm, 640nm) FACS Aria (BD Biosciences) into empty 96-well low profile PCR plates (Labcon) ...
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bioRxiv - Immunology 2024Quote: Mb1-YFP+ B-pDC (B220+ PDCA1+YFP+) and Mb1-YFP- pDCs (B220+PDCA1+YFP-) subsets were sorted on a FACS Aria (BD Biosciences) by and cultured in 96-well round bottom plates (n=3 wells in triplicates ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... and cell cycle analysis was performed by staining cells with BrdU-APC and 7-AAD with BD Pharmingen™ APC BrdU Flow Kits (552598, BD Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 7 and 14) or at 2 time points (days 1and 14) after vaccination into Vacutainer® Heparin tubes (BD Biosciences, Plymouth, U.K.). Blood samples were subjected to immediate preparation and analyses ...
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bioRxiv - Neuroscience 2021Quote: ... PCA on MELAS versus control samples was performed on data from 7 MEA parameters (MFR, PRS, BR, BD, BSR, IBI, and NBR), since many samples did not show network bursts (i.e ...
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bioRxiv - Immunology 2020Quote: ... All antigen specific CD8 T cells were phenotypically analyzed by Flow Cytometry after surface staining using monoclonal antibodies for Thy1.1(BD clone OX-7), CD45.1 (Biolegend clone A20) ...
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bioRxiv - Microbiology 2020Quote: ... The fundamental M9 media formulation (pH 7) was supplemented with 0.8% deferrated acid-hydrolyzed casamino acids (BD Biosciences San Jose, CA, USA), 8 mM MgSO4 ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and centrifuged at 1000rpm for 5-10min and stained with APC-Cy™7 Rat Anti-Mouse CD3 (clone: 17A2, Cat # 560590, BD Biosciences), FITC Rat Anti-Mouse CD4 (Clone ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C in a TC-7 roller drum (NB, Canada) at 240 rpm or on Lysogeny Broth (LB; BD Difco, Canada) agar plates ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cell lines were evaluated for expression of B7-H3 using B7-H3 antibody (clone 7-517, BD or clone FM276, Miltenyi).
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bioRxiv - Cancer Biology 2023Quote: ... PerCP-Cy5.5 or phycoerythrin-cyanine 7 (PE-Cy7)-conjugated CD4 (BD Biosciences Cat# 558170; BD Biosciences Cat# 550954, BD Biosciences Cat# 561099); Brilliant Violet (BV ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were serially diluted in saline and all dilutions (100 to 10-7) plated on LB agar plates (BD, USA, Product #244510) containing 0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Five 96-well plates were seeded with one GFP positive cell per well using the BD FACSAriaTM II (BD Biosciences) apparatus ...
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bioRxiv - Genetics 2020Quote: ... One ml of the epididymal cell-containing medium was carefully filtered through a 100 μm cell strainer (BD Biosciences, Inc.) into a 1.5 ml microfuge tube prior to counting using a Hemocytometer chamber ...
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bioRxiv - Cell Biology 2022Quote: ... One milligram of cell lysate was incubated with mouse normal IgG or G3BP1 antibody (BD, Franklin Lakes, New Jersey, 611126) for 8 h with protein A/G agarose beads (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... with LCMV Cl13 and BrdU detection in splenic P14 cells was performed one day later (d8p.i.) using a BrdU detection Kit (BD Bioscience) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... cells were subjected to one-step fixation/red blood cell lysis (eBioscience #00-5333-57) before acquisition on FACS Celesta (BD). Determination of AH1 reactive cells out of CD8+ T cells were determined using FlowJo Software (version 10.8.0) ...
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bioRxiv - Cell Biology 2023Quote: ... and GFP-positive cells were FACS-sorted into 96-well plates at one cell per well using FACSMelody-Cytometer (BD) operated by FACSDiva 9.1.2 software ...
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bioRxiv - Cell Biology 2023Quote: ... Clonal populations were then iso-lated by seeding dual BFP and mCherry-positive cells into a 96-well plate such that only one cell was deposited in each well using a FACSAria Fusion (BD). The cells were then allowed to grow up and clonality was verified by genomic DNA sequencing of the cut site as previously described Graziano et al ...
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bioRxiv - Immunology 2021Quote: ... To detect sheep RBC-induced GC, DZ and LZ B cells, splenocytes were incubated with directly conjugated monoclonal antibodies (Anti-CD19-PE, (BD Bioscience, 7073631), anti-B220-BV510 (BioLegend ...
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bioRxiv - Biophysics 2020Quote: Spectrin in neurons (Figure 2d and Supplementary Figures 2 and 5) was labelled with a mouse monoclonal primary antibody anti-β-Spectrin II (Clone 42/B-Spectrin II, BD Biosciences) for 1 h at room temperature using a 1:400 dilution in 5% BSA in PBS ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining for IFNγ and granzyme B was conducted in Perm/Wash buffer after permeabilization in Fix/Perm buffer (BD Pharmingen, USA) according to kit instructions.
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bioRxiv - Microbiology 2024Quote: ... Stained memory B cells (MBCs; CD3/CD14/CD56- CD19+ CD27+ IgD- IgM-) were single-cell sorted with a BD FACSAria™ Fusion (BD Biosciences) into 384-well microplates containing 3T3-CD40L feeder cells and were incubated with IL-2 and IL-21 for 14 days71.
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bioRxiv - Biochemistry 2019Quote: ... After 6 or 7 days cells were resuspended in enzyme-free EDTA buffer and replated in untreated 6-well plates (BD Falcon, Sigma-Aldrich) at 1 M cells per well in unconditioned medium with or without priming in 25 U IFN-γ (PeproTech ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... α7 integrin-PE antibody (AbLab, Vancouver, Canada), CD9-APC antibody (eBioscience, San Diego, CA) and CD47-BV605 antibody (BD Biosciences, San Jose, CA) for 30 minutes on ice and washed ...
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bioRxiv - Cancer Biology 2019Quote: ... For apoptosis analysis was performed by a double staining Annexin V and 7-AAD Apoptosis Detection Kit (BD Pharmingen, San Jose, CA, USA) following the manufacture’s introductions ...
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bioRxiv - Immunology 2020Quote: ... 7 days post-vaccination) and measured Cytokines using the BD CBA Mouse Th1/Th2/Th17 Cytokine Kit (BD Bioscience, San Jose, CA, USA). Sera samples were processed as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cell pellets from the single-cell suspension were resuspended in FACS buffer (PBS, 5% FBS, and 5 mM EDTA) for staining in ice for 10 min with 7-AAD (BD Biosciences, #51-68981E). Only 7-AAD- cells were considered as viable cells ...