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Citations for Becton, Dickinson and Company :
2001 - 2050 of 7027 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... at which point they were phenotyped (FOXP3-PeCy7, clone FJK-16s, Thermo Fisher Scientific; HELIOS-PEDazzle594, clone 22F6, Biolegend; Thy1.1-BV711, clone OX-7, BD Biosceinces ...
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bioRxiv - Cancer Biology 2024Quote: NF1-proficient and NF1-deficient engineered clones were injected into the flanks of 6- to 7-week-old athymic nude mice (1.0×106 cells) in 50% Matrigel (BD Biosciences). Mice were monitored for tumor formation for 6 months post injection or until a tumor volume of 2000mm3 was reached.
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bioRxiv - Microbiology 2021Quote: One milliliter of this mixture was then added to 9 ml of Enterococcosel broth (BD, Mississauga, Ontario, Canada) with or without 8 μg ml−1 erythromycin (Sigma Aldrich Canada ...
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bioRxiv - Immunology 2021Quote: ... and pre-cultured MS40L-low feeder cells at one cell per well using FACS Symphony S6 (BD Biosciences). The plates were incubated at 37 °C in a humid atmosphere with 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... Half of the enriched B cells were stained with anti-rat IgM (Clone G53-238, BD Biosciences, San Jose, CA) conjugated to PE Cy7 ...
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bioRxiv - Immunology 2019Quote: ... cells were washed and permeabilized for 16 hours according to manufacturer’s instructions for staining with anti-FOXP3 (236A/E7, PE, eBioscience) and anti-Granzyme-B (GB11, BV421, BD Biosciences). Cells were then fixed for subsequent analysis on a Fortessa flow cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... Naive and germinal center B cell purity (>90%) was determined by flow cytometry analysis of surface IgD (BD Biosciences 555778), CD77 (BioRad MCA579 ...
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bioRxiv - Microbiology 2020Quote: ... B cell Ca2+ influx was monitored by measuring the 379/450 nm emission ratio on a LSR Fortessa (BD Biosciences). Following 30 s of baseline measurement ...
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bioRxiv - Microbiology 2021Quote: ... CD21 BV786 (Clone B-LY4, #740969), HLA DR BB700 (Clone G46-6, #566480), CD27 APC R700 (Clone M-T271, #565116) all from BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... and 9g4-naïve B cells (CD27− IgD+ 9g4−) were obtained by FACS sorting on a BD FACSAria II (BD Biosciences). Purified samples were pelleted and stored at −80°C.
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bioRxiv - Genomics 2019Quote: ... Dendritic cells and basophils were sorted as CD3−CD19−CD11c+HLA-DR+ (DCs) and CD3−CD19−CD123+ (basophils) using the following antibodies: CD11C-PECy7 (Clone B-ly6, BD), HLA-DR-APCCy7 (Clone G46-6 ...
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bioRxiv - Immunology 2019Quote: ... Primary human B cells: Human peripheral blood was collected by routine phlebotomy in heparinized vacutainers (BD Biosciences, San Diego, CA) and diluted 1:2 with PBS containing 2% FBS ...
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bioRxiv - Immunology 2020Quote: ... CD19+CD27+IgG+ memory or CD19+CD27-IgG- naive B cells were isolated using an Aria II sorter (BD Biosciences). The sorted B cells were then added to the irradiated CD40L+ 3T3 cells at a density of 500-25000 cells/well ...
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bioRxiv - Immunology 2022Quote: ... DOT cells were incubated after membrane labelling with anti-perforin-BV241 (dG9) and anti-granzyme B-BV510 (GB11) from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Live Naïve B cells were obtained by sorting the stained cells using a FACS (fluorescence-activated cell sorting) Aria (BD Biosciences) as 7-AAD-CD19+CD38-IgD+CD27- ...
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bioRxiv - Immunology 2023Quote: ... transitional (CD19+CD3- B220+CD93+) and mature B cells (CD19+CD3-B220+CD93-) on BD FACSAria Fusion and FACSAria II (BD), before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2024Quote: ... the B cells were subjected to single-cell sorting using a BD FACSAria III (BD Biosciences, Franklin Lakes, NJ, USA) and collected into 96-well plates pre-loaded with lysis buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in ice cold PBS and supplied with 10 μl of 7-AAD to assess cell viability (BD Pharmigen) and sorted with a BD Aria III FACS as previously described 30 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 x g cells were carefully resuspended in cell culture medium with 10 µl/ml 7-AAD (BD Biosciences). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice and resuspended in 200 μL of blocking buffer in the presence of 7-AAD (BD Biosciences Cat#: 51-68981E) or DAPI (Invitrogen Cat# ...
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bioRxiv - Cell Biology 2022Quote: ... Cell survival was measured by FACS analysis after staining cells with a membrane-impermeable DNA binding dye (DAPI, PI, or 7-AAD) and/or annexin V (BD Pharmingen).
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bioRxiv - Developmental Biology 2022Quote: ... Cells from mouse tail skin (aging RNA-seq) and dorsal/ventral skin (fibulin 7 RNA-seq) were isolated using FACS Aria flow cytometer (BD Biosciences). Data were analyzed using FlowJo software (BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Myoblasts were stained with 7-AAD (cell viability) and with the positive selection marker anti-CD56 AF647-conjugated (clone R19-760, BD biosciences). Of note ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with 7-AAD (live/dead marker) prior to flow cytometric sorting on an FACS Aria II sorter (BD Biosciences). The sorted germinal centre B cells (7-AAD−/B220+/GL-7+/Fas+ ...
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bioRxiv - Immunology 2021Quote: ... and then labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, FITC anti-mouse CD4, APC-Cy®7 mouse anti-CD8, all from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: Mononuclear cells were directly labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, clone, FITC anti-mouse CD4, APC-Cy®7 anti-mouse CD8, for T lymphocyte phenotype, all from BD Biosciences ...
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bioRxiv - Genomics 2020Quote: ... ODInCas9 system was left to turn off for 7 days post transfection before assessment using FACS instrument BD LSRFortessa (BD Bioscience) and confocal microscopy instrument Cell Voyager 7000S (CV7000S ...
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bioRxiv - Immunology 2019Quote: ... NK92 cells or primary patient fibroblasts were incubated with BrdU for 12 hours prior to fixation and staining with 7-AAD (BD Biosciences). Cells were analyzed via FACS on a BD Fortessa with 18-color configuration and resulting data were evaluated using Kaluza (Beckman-Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The infectivity was measured by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and measured as the percentage of GFP positive cells analyzed with a Fortessa X20 flow cytometer (BD Biosciences) at 24 hours post infection ...
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bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
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bioRxiv - Cell Biology 2021Quote: ... macrophages were incubated with 5 μM 2’, 7’ dichlorofluorescein diacetate (DCFDA, Invitrogen at 37°C for 30 min, followed by acquisition using FACS Canto I (BD Sciences) within 30 minutes of staining ...
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bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Infectivity was assessed by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and/or determined by the percentage of GFP(+) cells analyzed using a Fortessa X20 flow cytometer (BD Biosciences) at 16 ...
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bioRxiv - Immunology 2022Quote: ... Human CD34+ stem cell purity was monitored by anti-human CD34 FITC (clone:581) and 7-AAD viability dye using LSR Fortessa X-20 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 7 days and immunostained for flow cytometry (Antibodies: CD34, clone 581; CD117, clone: 104D2; CD11b, clone ICRF44 (BD Pharmingen) and 1:50 FcR Blocking Reagent (Miltenyi Biotec)) ...
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bioRxiv - Biochemistry 2023Quote: Cells were grown in synthetic complete medium at 25°C to mid-log phase after 7-8 doublings and harvested for direct fluorescent measurement of RFP and YFP channels by an LSR Fortessa (BD biosciences) flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... from naïve/PBS-treated or i.n fusion protein immunized mice were isolated from mLN from a pool of 7-8 C57Bl/6 mice and FACS-sorted on a Aria II cell sorter (BD Bioscience). Labeling of cells prior to sorting by flowcytometry (Fusion ...
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bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
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bioRxiv - Microbiology 2023Quote: Bacteria used in this work are listed in Supplementary Table 7 and were grown at 37 °C in brain and heart infusion (BHI) broth (with agitation) and agar media (BD Difco). Cell lines of human origin used in this work included JEG-3 trophoblasts (ATCC HTB-36) ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclear extract was prepared by dounce homogenization in cold swelling buffer (using a size 7 dounce) and filtered using the cell strainer (100 μm, BD Biosciences). Lysate was centrifuged at 400 g for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Cell apoptosis was measured by co-staining cells with Annexin V and 7-AAD using the PE Annexin V Apoptosis Detection Kit I (BD Biosciences), according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... OPs were obtained by immuno-panning from single-cell suspensions of dissociated P6-7 mouse cortices using anti-PDGFRA immunoglobulin (558774, BD Pharmingen) as previously described (Emery and Dugas ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% triton X-100) for one hour at room temperature and then immunostained with Rac1 (BD Transduction Laboratories, #610650) or zDHHC3 (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... One milliliter of these enrichment broths was transferred to 10 ml tubes of pre-warmed M-broth (BD-Difco) and incubated at 35°C for 6-8 hours ...
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bioRxiv - Microbiology 2019Quote: ... Tubes were placed in 2.5 liter AnaeroPack System rectangular jars (Mitsubishi Gas Chemical Co., Inc.) with one GasPak EZ sachet (BD). These cultures were grown for 5 days at 37°C prior to measuring OD600 using a Genesys 6 spectrophotometer.
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bioRxiv - Molecular Biology 2021Quote: ... One single cell per well was seeded in 96-well plates using the BD Biosciences FACSAria II (BD Biosciences) apparatus ...
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bioRxiv - Bioengineering 2022Quote: ... we connected one of the four fluidic quadrant outlets to a 60 mL syringe (BD Bioscience, San Jose, CA) and syringe pump (Fusion 200 ...
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bioRxiv - Microbiology 2023Quote: ... washed one time with PBS and resuspended in PBS for analysis on a LSR Fortessa flow cytometer (BD Biosciences) with BDFACSDiVa™ Software ...
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bioRxiv - Immunology 2024Quote: ... Gating and data analysis were performed either with unstained samples or fluorescence minus one control using FlowJo v10.8.1 (BD Biosciences).