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Citations for Becton, Dickinson and Company :
1501 - 1550 of 2202 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 15 min at 4°C with Fc-block (anti-CD16/CD32, 2.4G2, BD biosciences) before staining ...
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bioRxiv - Immunology 2020Quote: ... Surface antibody staining was then performed for 1hr at 4°C in Brilliant Stain Buffer (BD Biosciences #563794). Samples were acquired on an LSRFortessa 5 with stained UltraComp eBeads Compensation Beads (Invitrogen #01-2222-41 ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS (pH 7.4) for 15 min at 4 °C and analyzed with BD LSR Fortessa (BD Biosciences) flow cytometry ...
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bioRxiv - Cell Biology 2022Quote: ... cells were stained with 50 µl of Alexa-Fluor 647 conjugated anti-Cytochrome-C antibody (1:500, BD) at room temperature for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... for 3 hours at 37°C in the presence of GolgiStop (monensin, according to manufacturer’s recommendation, BD Biosciences). Cells were washed and labelled with fixable viability dye (LIVE/DEAD eFlour780 ...
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bioRxiv - Microbiology 2022Quote: ... pyogenes were grown at 37°C in a screw-cap centrifuge tube (BD Biosciences, San Jose, CA, USA) filled with Todd-Hewitt broth supplemented with 0.2% yeast extract (THY ...
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bioRxiv - Microbiology 2022Quote: ... faecalis strain OG1RF (86) was cultured aerobically at 37°C in Mueller-Hinton (MH) broth (BD Life Sciences) supplemented with 50 μg/mL rifampin (Rif ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were cultured on Tryptic Soy agar plates or in filter-sterilized Tryp-c Soy broth (BD Diagnostics). All cultures were incubated at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Stock cultures were routinely grown at 37°C to OD550∼0.3 in Todd-Hewitt medium (BD Diagnostic System) supplemented with 0.5% Yeast Extract (THY ...
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bioRxiv - Microbiology 2023Quote: ... 3 loopfuls of biomass grown at 30°C on Brain-Heart Infusion (BHI) agar (BD Biosciences, NJ, USA) were collected and mixed with a phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2024Quote: ... gently fixed at 4°C for 2-4 hours in Cytofix (diluted 1:4 in PBS; BD biosciences), then embedded in O.C.T ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight at 4 °C with antibodies specific for E-cadherin (BD Biosciences, Franklin Lakes, NJ, USA), K ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies used included: C terminal binding protein-2 (mouse anti-CtBP2; BD Transduction Labs, used at 1:200), myosin-VIIA (rabbit anti-myosin-VIIA ...
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bioRxiv - Cancer Biology 2023Quote: ... for 15 min and incubated overnight at 4°C with the primary antibody Ki67 (BD Biosciences, Cat# 558619) and followed staining protocol with DAB as done previously(16) ...
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bioRxiv - Molecular Biology 2023Quote: ... Electrocompetent pKD46+ cells were prepared from a culture grown at 30 °C in LB medium (1.0% bactotryptone (BD), 0.5% yeast extract (Alfa Aesar) ...
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bioRxiv - Microbiology 2023Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Neuroscience 2023Quote: ... 1:200) and (2) C-terminal binding protein 2 (mouse anti-CtBP2; BD Biosciences; used at 1:200) with secondary antibodies coupled to Alexa Fluors 647 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... see Supplementary Table 1) for 30 min at 4°C and washed twice in TF washing buffer (BD) and once in SB.
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated overnight at 4°C with a pair of antibodies – mouse antibodies recognizing L1 (BD Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated overnight at 4°C with anti-CD9 (1:1000, BD Pharmingen, San Jose, CA, USA), anti-CD63 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... All samples were incubated at 4°C with rat anti-mouse CD326 (EpCAM, BD Pharmingen, 552370, 1:1,000) and 10 μg/ml Hoechst 33342 (Molecular Probes/Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and incubated for another 15 minutes at 4°C in the presence of Fc receptor block (BD Biosciences). Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail ...
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bioRxiv - Cell Biology 2023Quote: Cells or purified ApoBDs were lysed at 4°C using ice-cold 1× cell lysis buffer (BD Biosciences). Protein concentration of the ApoBD lysates was determined using Pierce™ bicinchoninic acid assay (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... They were incubated overnight at 4 °C with mouse anti-BrdU antibody (1:500 dilution, BD Biosciences, 347580) under non-denaturing conditions followed by anti-mouse secondary antibody (1:1000 dilution ...
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bioRxiv - Immunology 2024Quote: ... The sections were stained overnight at 4°C with the following antibodies: BV421-labeled SIRP⍺ (P84, BD Biosciences), Pacific Blue–labeled B220 (RA3-6B2 ...
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bioRxiv - Systems Biology 2020Quote: ... White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
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bioRxiv - Immunology 2021Quote: Supernatants were collected 4 or 24 h post-challenge and the levels of TNF and mature IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems) ...
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bioRxiv - Immunology 2021Quote: CD47 expression or cross-dressing on cells was determined by direct staining with BV786-conjugated anti human specific CD47 mAb B6H12 (abbreviated anti-hCD47; BD Bioscience) or PE-conjugated anti-human CD47 mAb CC2C6 (with cross-reactivity to pig CD47 and thus ...
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bioRxiv - Microbiology 2020Quote: Macrophages were infected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using ELISA kits for human IL-1β (BD Biosciences) and IL-18 (R&D Systems).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
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bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
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bioRxiv - Immunology 2022Quote: ... NK cells were added to the washed ELISA plate and incubated together with anti-human CD107a (BD, 1:40, clone: H4A3), brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... primary hMDMs were transfected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using an ELISA kit for human IL-1β (BD Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2019Quote: ... with anti-positive (CD90, CD105, CD44) and negative marker (CD34, CD11b, CD19, CD45, MHC class II) antibodies (Human MSC Analysis Kit, 562245, BD Biosciences), as described in detail in [42] ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell suspensions from APEL vascular differentiations were incubated with mouse anti-human CD31-APC (eBioscience, San Diego, CA) and CD146-PE (BD Biosciences) antibodies for 30 min on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... The HLA-DQ cell surface expression was performed by cytofluorimetric analysis using the FACSAria III and DIVA software with FITC mouse anti-human HLA-DQ antibody (BD Biosciences). The quantitation of specific transcripts was performed by qRT-PCR as described in [9] ...
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bioRxiv - Cancer Biology 2019Quote: ... cKIT and PDGFRA expression was assessed using mouse anti-human CD117 (PE-Cyanine7, clone 104D2, #25-1178-42, eBioscience) and CD140a (PE, clone αR1, #556002, BD Pharmingen) respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked for 1 h in 5% milk/TBS Tween-20 solution and incubated for 1 h with anti-TOP2β (1:5,000, mouse anti-human, Clone 40/Topo IIβ, RUO – 611492, BD Biosciences-US) or anti-TOP2α (1:10,000 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed three times in PBS followed by incubation in 1% Human Fc Block (BD Biosciences, San Jose, CA) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with a 1:50 dilution of phycoerythrin (PE)-conjugated mouse monoclonal antibody against human TRAIL (550516, BD Biosciences). For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770 ...
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bioRxiv - Bioengineering 2020Quote: ... Cytokine concentrations in the culture supernatant were measured using BD Cytometric Bead Array Human Th1/Th2 Cytokine Kit II (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Cytokines in the serum sample were measured using human Th1/Th2/Th17 cytometric bead array kit (BD Bioscience, San Jose, CA) according to the manufacture’s instruction ...
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bioRxiv - Immunology 2021Quote: ... labelled with anti-human myeloid and stromal markers (Table 1) and analysed by flow cytometry on a LSR Fortessa (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... Human CD34C-derived ECs were seeded at a density of 8×104/insert onto the Matrigel-coated (BD Biosciences, United States) Transwell inserts (3401-Costar ...
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bioRxiv - Cancer Biology 2019Quote: ... and human prostate cancer propagating cells were resuspended in 120 µl of a 1:1 mixture of Matrigel (BD Biosciences, #354234) and PrEGM (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... adjusted to 106 CFU ml-1 in HBSS and 104 CFU (10 μl) used to inoculate 90 μl of freshly donated human blood (collected in EDTA-treated tubes; BD Biosciences) or freshly diluted H2O2 (10 mM in PBS ...
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bioRxiv - Microbiology 2020Quote: ... IL-10 and IL-12p70 were quantified in those culture supernatants by ELISA with BD OptEIA™ human IL-10 and IL-12p70 sets following the manufacturer's instructions (BD Bioscience).
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...