Labshake search
Citations for Becton, Dickinson and Company :
1401 - 1450 of 1509 citations for Mycoplasma Agar 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... total asthmatic lung cells (0.5 million) were cultured in flat bottom 96-well plates coated with 1 µg/ml of anti-CD3 (145-2C11, BD Biosciences) and 1 µg/ml anti-CD28 (37.51 ...
-
bioRxiv - Microbiology 2024Quote: ... the cells were resuspended in Facs-Buffer and PscF or PcrV positive B cells are sorted and clonally seeded in 96 plates containing lysis buffer using BD FACSAria Fusion cytometer (BD Biosciences).
-
bioRxiv - Molecular Biology 2024Quote: ... GFP- (PX458) or mRuby-positive (PX461) cells were single cell sorted into 96-well plates using a FACSAria™ III sorter (BD).
-
bioRxiv - Bioengineering 2024Quote: ... 25,000 irradiated HM-1-luc cells were mixed with 3 × 105 PBMCs per sample and seeded in a 96-well ELISPOT plate (BD Biosciences) that was pre-coated with IFN-γ capture antibody (BD Biosciences) ...
-
bioRxiv - Immunology 2024Quote: ... positive for wild type heterologous SOSIP probes and negative for V2 apex epitope mutant probes were sorted at 1 cell per well into 96-well plates on a BD FACSAria II machine using BD FACSDiva software (BD Biosciences). RNA extraction ...
-
bioRxiv - Systems Biology 2024Quote: - selection of diploids twice on SC(MSG)-Ura + G-418 plates (1.7 g/l yeast nitrogen base without amino acids and ammonium sulfate (BD Biosciences, 233520), 1 g/l monosodium glutamic acid (MSG ...
-
bioRxiv - Systems Biology 2024Quote: - N-SWAT and C-SWAT libraries were assembled in 384-colony format on SC-Ura plates (6.7 g/l yeast nitrogen base without amino acids (BD Biosciences, 291940), 2 g/l amino synthetic complete acid mix lacking uracil (SC-Ura) ...
-
bioRxiv - Neuroscience 2024Quote: ... A human EC model of obesity and diabetes was established by growing HUVECs to confluency on gelatin-coated 24-well TC plates or glass chamber slides (4-well or 8-well; BD Falcon) in EGM-2 and thereafter changing to growth factor-depleted (GFD ...
-
bioRxiv - Molecular Biology 2024Quote: ... selected with Puromycin and seeded surviving cells as single cells in 96 well plates by FACS (BD FACSAriaTM Fusion Flow Cytometer). Resulting clones had an insert of 282 bp in between FRT/F3 sites and were genotyped for the floxed CTCF motif by PCR and validated by Sanger sequencing ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were transiently transfected with the specific sgRNA plasmid or empty PX458 plasmid using PEI (HeLa) and sorted single-cell into 96 wells plate 48h PTF using a BD Aria III cell sorter (BD-Bioscience). Clones were screened by western blot for protein expression and Sanger sequencing for the mutation at the intended locus ...
-
bioRxiv - Genomics 2024Quote: ... cells were detached and moved to a V-bottom 96-well plate for flow cytometry analysis using a FACSymphony A5 flow cytometer (BD Bioscience) equipped with a high-throughput plate sampler and FACSDiVA software (BD Bioscience) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Individual KO clones were generated by sorting the bulk of antibiotic-selected cells into single cells in 96-well plates through flow cytometry (BD FACSAria), followed by amplification of the clones.
-
bioRxiv - Cancer Biology 2024Quote: ... and individual BFP positive cells were sorted into single wells of a 96-well plate using a BD FACSAria Fusion (BD Biosciences). Landing pad lentivector pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3 was a gift from Kenneth Matreyek (Addgene plasmid # 171588 ...
-
bioRxiv - Developmental Biology 2022Quote: ... they were distributed into V-bottom 96-well plates and treated with library antibodies for 30 minutes on ice (BD Biosciences, 560747). After two washes ...
-
bioRxiv - Immunology 2022Quote: ... were picked from a selective plate and inoculated in 1 mL SD-UT medium [yeast nitrogen base-casamino acids (YNB-CAA) (BD Biosciences 223120) + 2% D-(+)-Glucose (Sigma G5767-500G ...
-
bioRxiv - Bioengineering 2021Quote: ... albicans fungal cells (CMM16) were inoculated from a streaked plate in 2 ml yeast extract peptone dextrose (YPD) [1% yeast extract (BD Biosciences, 212730), 2% peptone (BD Biosciences ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were grown on 6-well plates until 80-90% confluency in presence or absence of 10 μg/ml 9EG7 antibody (BD Biosciences, #553715) for 24 h and scraped off manually with a rubber Corning Cell Lifter (Corning ...
-
bioRxiv - Bioengineering 2021Quote: ... To assess activation marker status T cells were stimulated with 0.1 μg/mL of D117C or PIGF on collagen coated plates for 16-20 hr and surface-stained with anti-human CD3 BUV395 (BD clone SK7), CD4 BV785 (Biolegend clone RPA-T4) ...
-
bioRxiv - Microbiology 2020Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were labeled with murine antibodies against CD3 (BV650) ...
-
bioRxiv - Microbiology 2021Quote: ... was plucked from each MOX plate and added to 5 mL of Brain Heart Infusion (BHI; BD Bacto, Franklin Lakes, New Jersey) broth and incubated at 25°C in a shaking water bath for 24 h ...
-
bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
-
ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
-
bioRxiv - Molecular Biology 2020Quote: ... A tube formation assay was performed by seeding HAEC in 96-well plate coated with Matrigel with reduced growth factors (BD Biosciences, CA) in the presence of human VEGF (10-20 ng/mL ...
-
bioRxiv - Cancer Biology 2020Quote: ... single cells positive for tdTomato were sorted under sterile conditions into 384-well plates containing OCI-C5x-conditioned media (50 µl/well) using fluorescence-assisted cell sorting (FACSAria cell sorter; BD Biosciences, Inc.). OCI-C5x conditioned media was prepared by seeding 7.5×105 OCI-C5x cells in a 10cm plate with 10ml OCMI-L media and collected 48 hours after incubation ...
-
bioRxiv - Microbiology 2020Quote: ... cells were analysed using fluorescence-activated cell sorting (FACS) and sorted at 20 cells per well in 96-well plates using a BD FACSAria™ III (BD Biosciences). Two cell phenotypes were sorted ...
-
bioRxiv - Immunology 2021Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 h of incubation ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... reduced to a single-cell suspension and seeded into a 96-well plate at a density ranging between 1000 – 20 c/w in mTesRTM1 supplemented with 1 nM ROCK inhibitor (BD Biosciences, 562822). Alternatively ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were passaged on plates pre-treated with 0.01% w/v poly-L-ornithine and coated with 10 ng/ml laminin (BD Biosciences, cat. 354232).
-
bioRxiv - Genetics 2020Quote: Both live cells and fixed cells were sorted into 96 well plates containing cell lysis buffer using BD Aria™ IIIu sorter (BD Biosciences). FSC parameters were used for singlet selection ...
-
bioRxiv - Microbiology 2022Quote: ... BALF cells were then plated in a 96-well round-bottom plate and restimulated using BD-Leukocyte Activation Cocktail containing BD GolgiPlug™ (BD Biosciences) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: The plate was incubated 6 hours at 37 °C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 hours of culture ...
-
bioRxiv - Genomics 2023Quote: ... were then sorted into a 384-well plate containing 5 μL of autoclaved source water using FACS (BD FACSMelody™ Cell Sorter, BD Biosciences). 10 µL of RLT+ lysis buffer (Qiagen ...
-
bioRxiv - Microbiology 2024Quote: ... Cells (1x106 cells/mL) were cultured in a 48-well plate pre-coated with anti-CD3 monoclonal antibody (BD Pharmingen, 5μg/mL), and culture supernatants were collected after 72h of stimulation and stored at -80°C until assayed for cytokine production ...
-
bioRxiv - Microbiology 2022Quote: ... and transferred to a 96-well round-bottom plate (TPP Techno Plastic Products AG, Switzerland) for automatic sampling by fluorescence-activated cell scanning (BD FACSCanto II).
-
bioRxiv - Microbiology 2024Quote: ... and HIV-1 NL4-3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
-
bioRxiv - Microbiology 2024Quote: ... as well as HIV-1 NL4.3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
-
bioRxiv - Cell Biology 2024Quote: ... and anti-CD28 antibodies (eBioscience, clone 37.51, 1 µg/ml) on TC plates pre-coated with anti-hamster antibody (BD Pharmingen, G94-56), and IL-2 (Immunotools ...
-
bioRxiv - Cell Biology 2023Quote: ... or 48 hrs (MDA-MB-231) prior to sorting mNeon-green positive cells into 96 well plates using the FACSMelody™ (BD Biosciences). Single cell clones were expanded and screened by PCR for homozygous knock-in of the DFN cassette.
-
bioRxiv - Cell Biology 2023Quote: ... plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD, 354277; Fisher Scientific, 140675). For the first 24 hours after passaging ...
-
bioRxiv - Microbiology 2023Quote: ... aeruginosa was maintained on TSA plates at 4°C and an inoculum was prepared by transferring a touch from this plate into 10 mL of tryptic soy broth (BD Bacto™) overnight ...
-
bioRxiv - Biochemistry 2022Quote: ... Splenocytes were stimulated with plate bound anti-CD3 and exogenous anti-CD28 antibodies (10μg/ml and 1μg/ml respectively; BD Biosc, San Diego, CA) for 2 days ...
-
bioRxiv - Bioengineering 2022Quote: Engineered cells were cultured for 3-weeks and flow sorted to dispense single GFP-positive cells into individual wells of 96-well plates in a BD FACSAria™ III (BD Biosciences). NALM6-GFP stable cells from a single colony were expanded ...
-
bioRxiv - Cancer Biology 2023Quote: ... Live single-cells were sorted directly into medium-filled wells of a 96-well plate using either BD FACSAria™ Fusion Flow Cytometer (BD Biosciences) or MoFlo XDP Cell Sorter (Beckman Coulter).
-
bioRxiv - Cell Biology 2023Quote: ... NIH/3T3 cells were transfected with pSpCas9(BB)-2A-GFP:sgRNA-RhoB and single GFP-positive cells sorted into 96-well plates via FACS using Aria-II SORP (BD Biosciences, Heidelberg, Germany). Cells were expanded and single clones screened for the absence of RhoB expression via immunoblotting ...
-
bioRxiv - Microbiology 2024Quote: ... the stromal vascular fraction was sorted into 96-well plate at 1 cell per well using BD FACSAria II Cell Sorter (BD Biosciences, USA). The cells were spontaneously immortalized by passaging for more than 30 passages ...
-
Langerhans cells regulate immunity in adulthood by regulating postnatal dermal lymphatic developmentbioRxiv - Immunology 2024Quote: Cells isolated from crawl-out epidermal cultures were washed with PBS and rested in serum-free media with 2 x 105 cells per well in 96-well assay plate (BD falcon, #353910) for 30 min ...
-
bioRxiv - Microbiology 2024Quote: ... The plate was incubated 6 h at 371°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 41h of incubation ...
-
bioRxiv - Immunology 2024Quote: ... in a 96-well U-bottom plate in an incubator for 6 hours in the presence of GolgiStop (BD Biosciences, cat#554724) and GolgiPlug (BD Bioscience cat#555029) ...
-
bioRxiv - Immunology 2024Quote: ... Assays were performed on Nunc MaxiSorp flat bottom 96-well plates (Thermo-Fisher, 44-2404-21) and developed with the addition of 100 μL of BD OptEIA TMB Substrate (BD Biosciences, 555214). Optical density was measured using a spectrophotometer (BIO-TEK ...
-
bioRxiv - Systems Biology 2024Quote: ... and lawns of pAWP78-P89-20ZRfae in E.coli S17-1 (overnight) were prepared for mating on plates with Hypho media supplemented with 5% Nutrient Broth (NB Difco, BD Life Sciences). Plates were incubated for 1 day at 30° C ...