Labshake search
Citations for Becton, Dickinson and Company :
1201 - 1250 of 1509 citations for Mycoplasma Agar 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... cells were trypsinized and re-plated in 96-well plates at a density of 0.5 or 1 cell per well using a FACSAria Fusion instrument (BD) to obtain single cell clones ...
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bioRxiv - Cell Biology 2023Quote: ... single GFP-positive cells were sorted into 96-well plates using a DiVa fluorescence-activated cell sorter (BD Biosciences) at the Duke Cancer Institute Flow Cytometry Shared Resource ...
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bioRxiv - Microbiology 2023Quote: ... T-cells were transferred to a 96 well plate after co-culturing and surface staining of CD8 (BD Biosciences) was performed ...
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bioRxiv - Immunology 2023Quote: Purified CD4+FYP+ Treg cells (1 × 105) were stimulated the plate-bound α-CD3 (1 μg/ml) (BD Pharmingen), α-CD28 (1 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... a single bacterial colony from a freshly streaked plate was grown overnight at 37 °C in low salt LB medium (LS-LB: 1.0% bactotryptone (BD), 0.5% yeast extract (Alfa Aesar) ...
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bioRxiv - Immunology 2023Quote: ... plates were washed three times again followed by incubation with biotinylated anti-IL-6 (1:500, BD Biosciences, #554402) and anti-TNF antibodies (1:250 ...
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bioRxiv - Cancer Biology 2023Quote: Matrigel-coated (invasion) 8 μm pore size translucent 24-well plate transwell chambers (BD Biosciences, San Jose, CA, USA) were used to evaluate the migration and invasion potential of melanoma cells cultured in different conditions ...
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bioRxiv - Immunology 2023Quote: ... plates were washed three times again followed by incubation with biotinylated anti-IL-6 (1:500, BD Biosciences, #554402) and anti-TNF (1:250 ...
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bioRxiv - Microbiology 2023Quote: ... and a batch of 30 96-deepwell plates were brought to the fluorescence activated cell sorter (FACS) (BD FACSJazz), and a restrictive gate based on FSC ...
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bioRxiv - Genomics 2023Quote: ... and single isolated nuclei (0.1% NP-40 substitute lysis buffer48 were sorted into 96-well plates using the BD FACSMelody and BD Fusion cell sorter ...
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bioRxiv - Genomics 2023Quote: ... and single isolated nuclei (0.1% NP-40 substitute lysis buffer48 were sorted into 96-well plates using the BD FACSMelody and BD Fusion cell sorter ...
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bioRxiv - Cancer Biology 2023Quote: ... GFP-positive single cells were isolated and sorted into 96-well plates by FACS Aria cell sorter (BD Biosciences). Cells were maintained in 96-well plates until they reached confluence and then expanded into larger plates for further analysis ...
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bioRxiv - Immunology 2023Quote: ... 2×106 cells/well (6-well plate) were cultured with 1μg/mL of anti-CD3 mAb (553057, BD Biosciences) plus 10 ng/mL of mouse IL-2 (Z02764 ...
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bioRxiv - Bioengineering 2023Quote: ... labeled wild-type HUVECs in a 1:1 ratio and seeded at a final density of 7.5 X 104 in 24-well plates coated with collagen 1 (from rat tail, BD). After culturing for 48h in complete medium at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... fluorescence-positive capsules were sorted into 384-well plates using a BD FACSMelody cell sorter (BD Biosciences, Tokyo, Japan) equipped with a 488-nm excitation laser.
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... Overnight cultures were diluted at OD600=0.2 in 6 well polystyrene plates (TPP) in 2 mL of minimal medium (SD: 2% glucose, 0.67% yeast nitrogen base without amino acids (BD), pH 5.4 ...
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bioRxiv - Cell Biology 2024Quote: ... and single cells were sorted by FACS into 96-well plates using a FACSAria III cell sorter (BD Biosciences) to obtain single-cell-derived clones ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed and incubated with detection antibody and enzyme reagent for 1 h at room temperature (BD Bioscience). TMB (Sigma ...
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bioRxiv - Immunology 2024Quote: ... in a 96-well flat bottom plate pre-coated with 2.5 µg/ml anti-CD3 (145-2C11, BD Biosciences) and 1 µg/ml anti-CD28 (37.51 ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed and incubated with detection antibody and enzyme reagent for 1 h at room temperature (BD Bioscience). TMB (Sigma ...
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bioRxiv - Immunology 2024Quote: ... T cells were activated for 2 days in plates coated with anti-CD3 (OKT3) and anti-CD28 (CD28.2) antibodies (BD) and transduced with CAR-encoding lentiviral particles at a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2024Quote: One million splenocytes in 200µL cRPMI/well were plated in a 96-well Microtest U-bottom plates (BD Falcon). Different conditions were plated in duplicate ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were quickly scraped into quenching buffer and transferred to a pre-chilled V-bottom polypropylene plate (BD 53263) on dry ice ...
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bioRxiv - Microbiology 2020Quote: ... Experimental starters were prepared from frozen master stocks struck on 5% Columbia blood agar plates (Biomerieux Ref# 43041) and grown overnight at 37°C with 5% CO2 prior to outgrowth in Todd-Hewitt (BD) broth supplemented with 50 mM HEPES (Sigma ...
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bioRxiv - Genetics 2021Quote: ... 250 µl of cells were transferred into 96-well plates and 30,000 cells were measured in a Fortessa X20 Flow cytometer (BD Biosciences), using the HTS plate mode on the DIVA software and a 488 nm excitation laser to capture the SytoxGreen DNA staining.
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bioRxiv - Molecular Biology 2021Quote: ... Five 96-well plates were seeded with one GFP positive cell per well using the BD FACSAriaTM II (BD Biosciences) apparatus ...
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bioRxiv - Immunology 2021Quote: Purified CD4+ T lymphocytes were in vitro activated with plate-coated anti-CD3 and anti-CD28 antibodies (1µg/mL, both BD Biosciences) in the presence of specific recombinant cytokines and neutralizing antibodies ...
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bioRxiv - Immunology 2021Quote: ... at 100ng/mL and IL-2 (100IU/mL) or expanded by plate-bound anti-CD3 mAb at 2μg/mL (BD Pharmingen) and soluble anti-CD28 mAb at 1μg/mL (BD Pharmingen ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were activated by seeding in 96-well plates pre-coated with anti-mouse CD3e (1 μg/ml working concentration, Clone: 145-2C11, BD) and anti-mouse CD28 (2 μg/ml working concentration ...
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bioRxiv - Developmental Biology 2020Quote: ... individual GFP+ mESCs were sorted into 96-well plates coated with 0.3% gelatin using a FACSAria-II cell sorter (BD Biosciences). Fast-growing wells (containing >1000 cells/well ...
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bioRxiv - Microbiology 2020Quote: ... after which the plates were placed inside a 2.5 L sealed jar (BBLTM GasPakTM from BD Biosciences or from Merck) under micro-aerobic conditions created by using an oxygen-absorbing AnaeroGen sachet (Oxoid) ...
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bioRxiv - Cancer Biology 2020Quote: ... in PBS + 2.5% FBS and viable single cells were sorted and deposited directly onto the MS-5 seeded 96-well plates using the FACSAria II (BD). Single cells were cultured for 17 days and 100μl of fresh erythro-myeloid promoting media was added at day 8.
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bioRxiv - Cancer Biology 2022Quote: Wells with the highest proportion of target barcodes were revived into a 6 well plate and grown for 4 days before being single-cell sorted by BD FACSAria II into 96 well plate ...
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bioRxiv - Immunology 2022Quote: ... Cells (1 x 106/mL) were then recovered and activated for 3 days on plate-bound anti-CD3 (BD, #553058) and soluble anti-CD28 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... fentanyl-reactive B cells were defined as live fentanyl+decoy-CD19+ and sorted into 384-well plates containing lysis buffer using an ARIA II cell sorter (BD Bioscience and using the index-sort function).
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bioRxiv - Molecular Biology 2020Quote: ... We then collected the FBs and single cell sorted in to 96 well plate by flow cytometry (BD Arial II) and generated a clonal FB line expressing the CRISPRi machinery ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 1.0 x106 cells were added to the 6-wells plates and treated with anti-CD16/32 antibody (BD Biosciences) to block the Fc receptor ...
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bioRxiv - Microbiology 2021Quote: ... fluorescence-positive beads were sorted into 0.8 μL DPBS in 96-well plates using a FACSMelody cell sorter (BD Bioscience) equipped with a 488-nm excitation laser ...
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bioRxiv - Cancer Biology 2021Quote: ... HCC1806 cells were seeded at a density of 15,000 cells per well in a 48-well tissue culture plate coated with 125μl of Matrigel Growth Factor Reduced (BD Biosciences). Media was replaced every 72h and growth was monitored for 9 days ...
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bioRxiv - Bioengineering 2021Quote: CHO-SCas9 cells were seeded at 0.3 × 106 cells/mL in 1 mL media in 26 wells of 12 well plates (BD Biosciences). In 25 of the wells ...
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bioRxiv - Immunology 2022Quote: ... FLAG-DNAM-1-expressing human Tconv cells were stimulated for 12 h with plate-bound 0.25 μg/mL anti-human CD3 mAb (clone HIT3α, BD Biosciences) and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2 ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of cell lysate premixes were pipetted to another flat-bottomed 96-well reaction plate containing 100 μl reaction buffer with 270 nM type 2 RNase H-specific substrate (BD) using a multi-channel pipette ...
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bioRxiv - Immunology 2022Quote: ... FLAG-DNAM-1-expressing human Tconv cells were stimulated for 24 h with plate-bound 0.25 μg/mL anti-human CD3 mAb (clone HIT3α, BD Biosciences) and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2 ...
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bioRxiv - Immunology 2022Quote: ... Single-cell clones were sorted into a 96-well U-bottom plate via a BD FACSAria flow cytometer (BD Biosciences) at the Center for Regenerative Medicine (CRM ...
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bioRxiv - Molecular Biology 2020Quote: ... surviving cells were further subcloned by transferring individual GFP-negative cells to 96-well plates by Fluorescence-activated cell sorting using a FACSAria (BD). 20 clones were expanded and visually reassessed for GFP-inducibility in the presence of 100 μM CμSO4 ...
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bioRxiv - Microbiology 2021Quote: ... 1% Penicillin/Streptomycin) in a 48-well plate (Costar) coated with CD3 Abs (1 μg/ml; BD Biosciences, Clone UCHT1) and in the presence of soluble CD28 Abs (1 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... pure and mixed cells were cultured in 6-well plates and FACS sorted 3 days after mixing on a FACS Jazz system (BD). After a FSC/SSC gate doublets were excluded ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells were stimulated in the presence of plate-bound stimulator OKT3 at a final concentration of 2.8 μg/ml (mouse anti-human CD3 Clone OKT3 BD Biosciences). Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... 2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). PBMCs were stimulated or left unstimulated for 16hrs ...
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bioRxiv - Microbiology 2021Quote: ... solanacearum cells were grown in VDM + 30mM NO3- in 96-well microtiter plates in anaerobic pouches (BD, Franklin Lakes, NJ) in a 28°C shaking incubator at 225 rpm ...