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Citations for Becton, Dickinson and Company :
1251 - 1300 of 2597 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Fixed and permeabilized cells were washed twice with PBSA buffer and incubated for 2 hr at room temperature with Alexa Fluor 647-conjugated anti-STAT5 pY694 antibody (BD Biosciences, clone 47/Stat5) diluted 1:50 in PBSA buffer ...
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bioRxiv - Immunology 2024Quote: ... blocked with 2% BSA in phosphate buffer pH=7.4 for 30min at RT and incubated with mouse anti-human LAMP1 (BD Biosciences, Franklin Lakes, NJ, USA) for 1h at RT ...
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bioRxiv - Microbiology 2024Quote: ... Cells were enumerated using a Cellometer X2 cell counter (Nexcelom Bioscience, Lawrence, MA, USA) and resuspended in yeast nitrogen base supplemented with 2% glucose (Difco; BD, Franklin Lakes, NJ, USA), to obtain a concentration of 2.0 × 106 cells/ml ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial strains were prepared for agar overlay plating by streaking out from glycerol stocks onto 2% streak agar plates (BD Difco 2216 marine broth), and allowed to grow for one days at room temperature ...
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bioRxiv - Physiology 2022Quote: ... and BLCs (PE Mouse Anti-NKX6.1: 1:40, BD Biosciences Cat# 563023, RRID:AB_2716792; and AF647 Mouse Anti-C-Peptide: 1:200 BD Biosciences Cat# 565831 ...
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bioRxiv - Immunology 2023Quote: ... PBMC were co-cultured with peptides at 37°C for 6h with CD107a BV786 (BD Biosciences, H4A3) and 1μg/mL of CD28/CD49d costimulatory reagent (BD Biosciences) ...
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bioRxiv - Plant Biology 2023Quote: ... Bait sequences (w/o signal peptides) were fused with the GAL4 transcription factor binding domain (GAL4-BD) in the p97-BD ...
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bioRxiv - Immunology 2019Quote: ... Serum was collected by tail-tipping before LPS injection and 2 and 4 hours following injection for IL6 determination by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufactures instructions.
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bioRxiv - Immunology 2021Quote: ... cells were washed and resuspended in PBS/2% FBS for analysis on a five-laser flow cytometer (FACSymphony A3 Cell Analyser, BD Bioscience, San Jose, CA, USA). Data were analyzed with FlowJo software (TreeStar ...
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bioRxiv - Neuroscience 2020Quote: Neurons from 18-day-old rat embryos were dissected and dissociated using trypsin and plated at a density of 70,000 cells cm−2 in minimal essential medium (MEM) supplemented with 10% NU serum (BD Biosciences, Le Pont de Claix, France), 0.45% glucose ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at room temperature followed by overnight incubation at 37°C in antibodies to the following: (1) C-terminal binding protein 2 (mouse anti-CtBP2; BD Biosciences, used at 1:200). (2 ...
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bioRxiv - Immunology 2021Quote: ... and intracellular cytokines (PE anti-mouse IFN-γ, PE-Cy®7 anti-mouse TNF-α, APC anti-mouse IL-2, all from BD Biosciences, USA). The labeled cells were acquired with the BD FACSCanto™ II flow cytometer and analyzed with the program FlowJo v.10.6.2 (BD Biosciences).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an air-liquid interface (ALI) in a defined differentiation media [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), and 50 nM retinoic acid (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an ALI in a defined differentiation media supplemented with IL-13 [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), 50 nM retinoic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: The actinobacteria were routinely cultured from frozen spore preparations via inoculation into Difco International Streptomyces Project (ISP) medium 2 (ISP2) (Becton, Dickinson and Company, Spark, MD) and incubated at 28° C ...
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bioRxiv - Immunology 2020Quote: ... before being washed and re-suspended in PBS/2% FCS for analysis on a five-laser flow cytometer (LSR FortessaTM; BD Bioscience, San Jose, CA, USA). The data were analysed with FlowJo software (TreeStar ...
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bioRxiv - Pathology 2021Quote: ... SYN303 [aa 2-12 Cat# 82401, Biolegend], 1:500, non-amyloid ß component directed Syn-1 [aa91-99, Cat# 610787, BD Trasduction Laboratories], 1:500), Histone H3 ([Cat# 3638S ...
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bioRxiv - Cancer Biology 2023Quote: ... BD 560046. After washing twice, cells were fixed in PFA 2% for 20 min, washed, and acquired on a Fortessa BD LSRII Cell Analyzer (Becton Dickinson).
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (BD Pharmingen 553673 2 μg/ml) at 4 °C for 20 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then washed 3x in flow cytometry stain buffer and fixed with 2% PFA for 20 minutes prior to acquisition on a LSRII Fortessa (BD Biosciences, San Jose, CA, USA). Samples were analyzed with FlowJo V10 software (Figure S4).
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bioRxiv - Cancer Biology 2023Quote: ... or E0771 hPD-L1 (2 × 105 cells in 25 μL of medium mixed with 25 μL of Matrigel Basement Membrane Matrix [BD Biosciences, San Jose, CA, USA]) were injected into the mammary fat pad or flank ...
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bioRxiv - Immunology 2023Quote: ... 5.0 × 104 CD4+ T cells were serially diluted onto either α-IL-2 or α-IFNɣ sterile coated PVDF 96 well plates (BD Biosciences, NJ, USA). The CD4+ T cells were then activated for 24 or 72 h in complete DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... PMVECs were purified after two rounds of immune selection using magnetic beads coated with anti-CD31 and anti-ICAM-2 antibodies (BD Bioscience, Franklin Lakes, NJ, USA). mPMVECs were cultured and maintained in a medium containing 5 % FBS and EC growth supplement (ECGS ...
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bioRxiv - Genetics 2022Quote: ... The proportion of HbF-and HbS-positive RBCs were measured with an antibody recognizing HbF (Thermo Fisher for HUDEP-2 cells or BD Biosciences for primary cells) or HbS (BioMedomics) ...
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bioRxiv - Neuroscience 2023Quote: ... Microdissected pieces were immunostained with antibodies to the following: (1) mouse anti-C-terminal binding protein 2 (pre-synaptic-marker, BD Biosciences, 1:200, Cat # 612044), (2 ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclei were then stained with 4’,6-diamidino-2-phenylindole (DAPI) and flowed through a fluorescence activated cell sorter (FACS) (BD FACSCanto, BD Biosciences, Franklin Lakes, NJ, USA) to assess viability ...
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bioRxiv - Immunology 2019Quote: ... The MHC monomers exchanged with peptides were tetramerized in the presence of allophycocyanin (APC) conjugated streptavidin (BD Biosciences) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... by subcutaneous injection of 200mg MOG peptide 35–55 in complete Freund’s adjuvant (Mycobacterium tuberculosis at 3.75 mg/ml; BD) followed by injection of 500ng of pertussis toxin (Sigma ...
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bioRxiv - Immunology 2019Quote: ... SIVmac239 Gag Peptide Set #12364) in the presence of anti-CD28 (1 μg/mL, clone L293, BD Biosciences) and anti-CD49d (1 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... N or M peptide pools (10μg/ml) in the presence of anti-CD28 (1μg/ml; BD Biosciences, USA) and recombinant interleukin (IL)-2 (20U/ml ...
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bioRxiv - Synthetic Biology 2019Quote: ... with 2% (w/w) PEG-PFPE amphiphilic block copolymer surfactant (Ran Biotechnologies, 008-FluoroSurfactant) were loaded into separate syringes (BD, 1 mL and 5 mL, respectively) and injected with 23 gauge needles through PTFE tubing (0.022” ID ...
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bioRxiv - Cancer Biology 2021Quote: ... Clones were picked after 2–3 months of culture and expanded in the same medium on dishes coated with Collagen I (BD Biosciences, Le Pont de Claix, France) in humidified atmosphere with 5% CO2 at 37°C.
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bioRxiv - Physiology 2021Quote: ... cells were washed twice with FACS buffer (PBS plus 2% heat inactivated fetal bovine serum) and analyzed on a SORP 8 Laser BD LSR flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ).
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Biophysics 2024Quote: ... transferred in a V-bottom 96-well plates and co-cultured with K562 cells (ATCC) at 1:1 effector-to-target ratio in the presence of mouse anti-human CD107a Alexa Fluor 647 (BD Bioscience, clone H4A3, 2 uL/well) for 6 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... cells were re-stimulated with peptides for 6 hours in the presence of Leuko Act Cktl with GolgiPlug (BD) plus 50 IU/mL IL-2 ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies for flow cytometry were: rat anti-C-peptide-Alexa Fluor 647 (1:200, 565831, BD Pharmigen) and mouse anti-Glucagon-BV421 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... cells were re-stimulated with peptides for 6 hours in the presence of GolgiPlug and GolgiStop (BD Cat#550583) plus 50 IU/mL IL-2 ...
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bioRxiv - Immunology 2023Quote: ... and media was tested by ELISA according to the manufacturer’s instructions (BD OptEIA Human IL-10 ELISA set #3555157 and BD OptEIA Human IL-1B ELISA set 2 #557953).
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bioRxiv - Neuroscience 2023Quote: ... This was followed by staining for 30 min at 4°C with the appropriate antibodies: PE-Cy7 rat anti-mouse CD45 (cat # 561868, BD Biosciences, clone 30-F11, 2 ng/μl), BV421 rat anti-mouse CD11b (cat # 562605 ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions were stimulated with OVA peptide (SIINFEKL) 2µM 37°C for 4 hrs in the presence of Golgi Stop (monensin, BD Biosciences). Upon extracellular staining ...
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bioRxiv - Immunology 2021Quote: ... no stimulation and a peptide pool spanning the spike protein at a concentration of 1 μg ml−1 of each peptide in the presence of 1 μg ml−1 of anti-CD28 (clone CD28.2, BD Biosciences) and anti-CD49d (clone 9F10 ...
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bioRxiv - Immunology 2021Quote: ... live cells were separated by density gradient and then restimulated by incubation with irradiated APCs from C57BL/6 mice that had been pre-loaded with indicated concentrations of SIINFEKL peptide or by PMA (10ng/ml) and ionomycin (1μg/ml) in the presence of GolgiPlug (BD Biosciences) for 4 hours.
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bioRxiv - Immunology 2019Quote: ... Each obtained monomeric H-2Db/peptide complex (0.5 mg/ml) was tetramerized at a 4:1 ratio with streptavidin-PE (BD Biosciences).
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bioRxiv - Neuroscience 2022Quote: ... aged 8 weeks received subcutaneous injections to both flanks and base of the tail of MOG35-55 peptide (125μg total, Mimotopes) emulsified 1:1 (vol/vol) in Freund’s complete adjuvant (BD Cat# 263810) containing additional Mycobacterium tuberculosis (5mg/mL ...
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bioRxiv - Immunology 2020Quote: ... 1–2 × 106 cells were incubated with 1 μM of SARS-CoV-2 minimal epitope or pool of up to ten epitopes (1 μM/peptide) for 9 h at 37°C in the presence of protein transport inhibitor (GolgiPlug; BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Spleen and lung cells that were stimulated overnight with peptide pools (as described under ex-vivo stimulation and intracellular staining) were fixed and permeabilized with Cytofix/Perm solution (BD) and then stained for intracellular cytokines ...
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bioRxiv - Microbiology 2022Quote: EAE was induced by injecting 8 to 10-week-old female C57BL/6J mice with 150 mg MOG35-55 peptide (Genemed Synthesis) emulsified in complete Freund’s adjuvant (CFA) (BD Difco) per mouse subcutaneously in the flanks ...
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bioRxiv - Bioengineering 2022Quote: ... CD8+ T cells were stimulated with specific or irrelevant peptides at 5μg/mL (JPT Technologies) in the presence of Golgi Plug (BD Biosciences) in complete medium (RPMI medium supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Immunology 2023Quote: Lymphocytes isolated from the spleen or siIEL compartment of naïve QFLTg_WT or OT-1_WT chimera mice were cultured in vitro with 2µM of FL9 or SL8 peptide respectively together with GolgiPlug (BD Biosciences) for 4.5h ...