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Citations for Becton, Dickinson and Company :
1201 - 1250 of 2597 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4×105 events were collected per sample within 3 hours after staining on a LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Microbiology 2019Quote: Spiral plating was conducted using an Autoplate 400 Eddy Jet 2 spiral plater (IUL, Barcelona, Spain) onto Lactobacilli deMan Rogosa and Sharpe (MRS) agar (Becton, Dickinson and Company) supplemented with 1% cycloheximide (Remel ...
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bioRxiv - Systems Biology 2022Quote: ... coli strain JM109 (1 × 1010 colony-forming units (CFU)/ml) were cultured for various times in 2 ml of 2xYT liquid medium (BD Difco, Cat# 244020) containing various concentrations of pyridoxal 5’ -phosphate (pH adjusted to 7.0) ...
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bioRxiv - Microbiology 2020Quote: ... Cultures with vesicles were incubated for 2 hours at the normal growth temperatures and examined on an Influx flow cytometer (Cytopeia/BD, Franklin Lakes, NJ). Cells were excited using a blue 488 nm laser and monitored for chlorophyll (692/40 nm emission ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation for 2 hours at room temperature with primary antibody (SOX2 [1:200, EMD Millipore AB5603], NESTIN [1:200, BD Pharmingen #556309]). After washing five times with PBS/0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: The HLA-A2 status was confirmed by PBMCs staining with 2 μL of anti-HLA-A2 mAb (clone BB7.2) (BD Pharmingen, Franklin Lakes, NJ), at 4°C for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Between 2 - 4×105 events were collected per sample within 24 hours after staining on a LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were immediately split into 3 fractions: 2 ml were transferred to heparin and EDTA tubes respectively (both BD, Franklin Lakes, New Jersey). Heparin samples were then centrifuged (1000x g ...
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bioRxiv - Cancer Biology 2019Quote: ... A pure polyclonal population of dCas9-KRAB expressing cells was generated by 2 rounds of fluorescence activated cell sorting (FACS) gated on the top half of BFP positive cells (BD FACS Aria II). The performance of A375_i in knocking down endogenous genes was evaluated by individually targeting 3 control genes (ST3GAL4 ...
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bioRxiv - Cell Biology 2019Quote: The following antibodies were used for fluorescence microscopy: mouse IgG2B anti-human DC-SIGN at 2 μg/ml (DCN46, BD Biosciences, Breda, Netherlands), mouse IgG1 anti-human AZN-D1 at 2 μg/ml (Geijtenbeek et al. ...
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... approximately 200 μL cells were added into the flow tube containing 2 mL PBS and tested by the flow cytometry (BD, New Jersey, USA). Three independent assays were performed.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with Hank’s buffered saline solution and resuspended in 2mM EDTA/ 2% BSA/ PBS buffer containing [2µg/mL] propidium iodide (PI) (BD Biosciences, Cat. No. 556364) and [1µM] Calcein violet (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Female C57BL/6J mice aged 8 – 12 weeks were injected intraperitoneally with 2 ml each of 3% sterile thioglycollate (BD Biosciences, catalog # 211716) medium ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were diluted in basal medium containing 2% dextran-coated charcoal-stripped serum (DCC) (HyClone Laboratories, Logan, UT) and plated into culture dishes (BD Falcon, Bedford, MA). Cells were cultured for 24 hours in an incubator at 37°C with saturating humidity and 5% CO2.
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Immunology 2022Quote: ... Spleens were disrupted using a syringe in 2% FBS/PBS and passed through a 40 μm nylon cell strainer (BD, catalog no. 352340) to obtain a single-cell suspension ...
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bioRxiv - Microbiology 2023Quote: ... which were grown in solid Lysogeny broth (LB) medium (5.0 g L−1 yeast extract, 10 g L−1 Bacto tryptone, 2 % Bacto agar (BD Biosciences, Franklin Lakes, NJ), 5.0 g L−1 NaCl ...
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bioRxiv - Microbiology 2024Quote: ... the internal space of the chambers in which biofilms were incubated measured 5,000 μm × 500 μm × 70 μm (LxWxH). Segments of inlet tubing (Cole Parmer PTFE #30, cat. # 06417–11) attached to 27Gx1/2 needles (BD Precision, cat. # 305109) on 1 milliliter syringes (Brandzig ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences, 557735], and anti-CD107a [BD Biosciences, 560647]) by 20 minutes of incubation at room temperature ...
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bioRxiv - Pathology 2023Quote: ... Cells were diluted in basal medium containing 2% dextran-coated charcoal-stripped serum (DCC) (HyClone Laboratories, Logan, UT) and plated into culture dishes (BD Falcon, Bedford, MA). Cells were cultured for 24 hours in an incubator at 37°C with saturating humidity and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... All 5 samples were then pooled and stained for surface markers in 70μl of the antibody cocktail (Supplementary Table 2) in BD Horizon Brilliant Stain Buffer Plus (BD Biosciences, San Jose, CA) for 30 minutes at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... digested cells were resuspended in FACS buffer (2% FBS–PBS) and analyzed by a FACS Calibur flow cytometer (Becton, Dickinson and Company, USA). Compensation and analysis of results were performed using Flow Jo software.
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed for 10 min in 2% paraformaldehyde and were analyzed using a FACS CANTO II (BD Biosciences, Franklin Lakes, NJ, USA). For lentiviral transduction efficiency assessment ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... of both preparations was determined by titration onto target cell lines and quantification of the percentage of BFP+ cells 2–3 days post-transduction by flow cytometry (BD Biosciences LSR II).
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bioRxiv - Immunology 2023Quote: ... Naïve T cells were resuspended in complete medium (KOHJIN BIO DMEM-H (16003016) + 10% FBS) and activated in the presence of 2 μg/mL of anti-CD3 (BD Pharmingen, REF: 553058) and 2 μg/mL of anti-CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... 250,000-350,000 donor cells or the same volume of empty HBSS+2% FBS vehicle were intraperitoneally (IP) injected using a 30 G insulin syringe (BD Biosciences cat# 328431). Transplanted cells were allowed 2 weeks to engraft prior to SCI.
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bioRxiv - Immunology 2022Quote: ... the cells were stimulated with their cognate peptide gp33 (10−6 M) and Golgistop (BD, 554724) for 5 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and plated as dissociated cells (25 × 104 cells/well) on day −2 in a 6-well Matrigel-coated plate (BD Biosciences, San Jose, CA). On day −1 ...
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bioRxiv - Plant Biology 2019Quote: ... Chromosomal DNA was then stained by 4’,6-diamidine-2’-phenylindole (DAPI) at final concentration 2 μg/ml and the chromosome suspensions were analysed by FACSAria SORP II flow sorter (BD Biosciences, San Jose, USA) at rates of 1000-2000 particles/sec ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS 2% FBS and analyzed by flow cytometry using a FACSCanto and Novocyte flow cytometer (BD Biosciences and Acea Biosciences). The population of interest was gated according to its FSC/SSC criteria ...
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bioRxiv - Developmental Biology 2020Quote: Cryopreserved cells were thawed at room temperature and washed twice with 10 ml of FACS buffer (PBS and 2% FBS) and filtered through a 100 µM filter (BD BioSciences, cat no: 352360). Cell concentration was determined by hemocytometer ...
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bioRxiv - Immunology 2020Quote: 2 × 106 cells were stimulated for 4 h at 23°C with 40 ng ml−1 PMA and 1 μg ml−1 ionomycin in the presence of 2 μM Monensin (Golgi-Stop, BD Biosciences, Cat: 51-2092KZ). Cells were washed twice with FACS buffer and stained for extracellular markers ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean of the fluorescence intensity of bead-positive cells and dividing by 10,000.
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bioRxiv - Immunology 2022Quote: ... the cells were fixed with 2% PFA and stored at 4°C until the samples were acquired on a LSRII cytometer (BD Biosciences, Mississauga, ON, Canada). Data analysis was performed using FlowJo v10.5.3 (Tree Star ...
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bioRxiv - Immunology 2022Quote: Total white blood cells from 1-2 mL freshly collected blood in EDTA tubes were enriched through ammonium chloride lysis (BD Bioscience, catalog no. 555899), followed by centrifugation (300x g for 10min) ...
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bioRxiv - Biochemistry 2022Quote: ... either single GFP+ cells (to obtain clones 1 and 2) or 250 GFP+ cells (to obtain the pool) were sorted per well into a 96 well plate (BD FACSAria Fusion Cell Sorter). The two clones and the pool were expanded and the decreased ArgBP2 expression was confirmed by immunoblotting.
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bioRxiv - Immunology 2019Quote: ... The cells were then washed twice in media and resuspended in 2% paraformaldehyde until they were run on a BD LSRII Flow Cytometer (BD BioSciences, San Jose, CA). Flow data were analyzed using Flowjo version 10.5.3.
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed using 2% paraformaldehyde (PFA) solution for 30 minutes and permeabilization was performed using a 1X BD perm/wash solution (BD Biosciences, USA; cat#554723) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: All mycobacterial strains (Supplementary File 2 – Key Reagents) were grown in liquid culture containing Difco™ Middlebrook 7H9 Broth (BD Biosciences, San Jose, CA) and supplemented with 0.2 % (v/v ...
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bioRxiv - Immunology 2020Quote: ... Cells were collected from wells by pipetting with PBS (containing 2%FBS and 2mM EDTA) and data acquisition was performed on a BD Accuri C6 (Becton, Dickinson and Company, USA). All flow cytometry data were analyzed with FlowJo v10.1 (BD Biosciences).
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bioRxiv - Microbiology 2022Quote: ... After 19h incubation at 37°C, the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on a LSRII (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean fluorescence intensity (MFI ...
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bioRxiv - Neuroscience 2022Quote: ... approximately 1-2 mm apart along the same bundle of muscle fibers with the help of a 20G syringe tip (BD Microlance, Thermo Fisher Scientific). After the dental cement hardened ...