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Citations for Becton, Dickinson and Company :
1151 - 1200 of 2597 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... IL-2 and IFN-γ concentration secreted in cell supernatant were measured using sandwich ELISA according to manufacturer’s protocol (BD Biosciences, USA). Plates were read on a Varioskan Lux multimode microplate reader (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained for 1 hour at 4 °C with 2 μl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Pathology 2022Quote: ... the samples were washed and resuspended in PBS supplemented with 2% FBS for analysis on a five-laser flow cytometer (BD Biosciences, Fortessa). The data were analysed with FlowJo software (TreeStar).
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bioRxiv - Immunology 2022Quote: ... For stimulation ∼106 lymphocytes plated in medium containing 2% fetal calf serum and 1.5 μl/ml Golgiplug (BD Bioscience; San Jose, CA) were cultured with peptides each present at a final concentration of 2 µg/ml for 5 hr at 37°C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2020Quote: ... 7 and 14) or at 2 time points (days 1and 14) after vaccination into Vacutainer® Heparin tubes (BD Biosciences, Plymouth, U.K.). Blood samples were subjected to immediate preparation and analyses ...
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bioRxiv - Pathology 2020Quote: ... Tomato-positive and -negative cells differed by ∼2 orders of magnitude in tomato fluorescence with excitation of 554nm and emission of 581nm by Influx™ cell sorter (BD Biosciences).
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bioRxiv - Microbiology 2019Quote: ... for 1 h or 2 h and then subsequently incubated for 30 min on ice with an APC-conjugated rat anti-mouse IgG (BD Biosciences, CA). Stained cells were analyzed using a BD AccuriTM C6 Plus with a Sampler flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Samples were resuspended to a volume 2.5 mL with PBS with 2% FBS and 0.4% EDTA and placed on an Imag (BD Biosciences, San Diego, CA) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 5x 105 cells resuspended in 20µl PBS were locally injected into the fracture site of bone under anesthesia using a Hamilton syringe (Hamilton, USA) and a 30-gauge 1/2 needle (BD Biosciences, USA). X-rays were taken weekly using a digital X-ray machine (Faxitron X-Ray Corp. ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS+2% goat serum and cell cycle analysis was conducted using a BD FACS Calibur (BD Biosciences, UK).
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bioRxiv - Cancer Biology 2020Quote: ... two groups of NOD/SCID female mice were orthotopically injected in the mammary fat pad #4 with 2×106 cells of either ALDH1A3 overexpressing or vector control MDA-MB-231 cells (admixed with high concentration Matrigel, BD, Cat # 354262). Mice were euthanized 49 days post cancer cell implantation and the lungs were harvested ...
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bioRxiv - Neuroscience 2020Quote: COs were washed 2 times for 5 minutes at room temperature in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Cell Biology 2019Quote: ... A second round of purification was then performed with anti-rat Dynabeads coated with rat anti-mouse ICAM-2 (rat clone 3C4; BD Biosciences #553325). Cells were cultured in Endothelial Cell Growth Medium MV2 (PromoCell ...
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bioRxiv - Genetics 2019Quote: ... in PBS with 1 μg/ml bovine serum albumin (BSA and 2 mM Tris-HCl. Cells were analyzed on a BD LSRII flow cytometer (BD Biosciences, Inc) and data were analyzed using FlowJo software ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used for the blockade of B7-1- and B7-2-dependent CD28 costimulation were anti-mouse CD80 Clone 16-10-A1 (BD Pharmingen #553736) and anti-CD86 Clone GL1 (BD Pharmingen #553689) ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... cells were fixed for 10 min at room temperature with 2% Paraformaldehyde and analysed using LSRFortessa™ X-20 Cell Analyzer (BD Biosciences), LSR II Flow Cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Lymphocytes were incubated in 200 μl of a mixture of BD FACS™ Permeabilizing Solution 2 and BD FACS™ Lysing Solution prepared according to manufacturer’s instructions (BD Biosciences) for 8 min at RT ...
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bioRxiv - Immunology 2022Quote: ... then fixed with 2% paraformalin and kept on ice until acquisition on a BD Symphony A3 (Becton Dickinson BD; Franklin Lakes, NJ). Data was analyzed using FlowJo 10.8.1 (RRID:SCR_008520 ...
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bioRxiv - Cell Biology 2022Quote: ... Rings were fixed after 7 days with 2% PFA and stained with rat PECAM (dilution 1:100, Rat monoclonal, clone MEC 13.3; BD Biosciences, Cat# 557355). Rings were imaged in 3D using the ZEISS Lightsheet Z.1 microscope ...
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bioRxiv - Microbiology 2022Quote: ... the internal space of the chambers in which biofilms were cultivated measured 5000μm x 500μm x 70μm (LxWxH). Segments of inlet tubing (Cole Parmer PTFE #30, cat. # 06417-11) attached to 27Gx1/2 needles (BD Precision, cat. # 305109) on 1mL syringes (Brandzig ...
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bioRxiv - Physiology 2022Quote: ... Samples were stored at 10-15°C in 2 mL K2-EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 15 to 45 min later.
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bioRxiv - Physiology 2022Quote: ... The sample was stored at 10-12 °C in 2 mL EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 0.5-2.5 h later ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were blocked with 2% BSA in 0.1% tween TBS (TBST) for 1h and immunostained with mouse anti-paxillin IgG1 antibody (BD Transduction Laboratories 610052) (1:500 ...
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bioRxiv - Immunology 2024Quote: ... and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD, Cat#566385) for 30 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Positively transduced cells were selected with 2 µg/ml puromycin followed by a sterile sort for high GFP and mCherry expression (BD FACSAria II). Expression was maintained with 1 µg/ml puromycin in all experiments except where immune cells were needed.
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bioRxiv - Cell Biology 2024Quote: ... 10 menstrual endometrial fragments (diameter 1-2 mm) from donor animals were inserted using two 14G catheters (Insyte, Vialon, BD, Madrid, Spain) under laparoscopic guidance in both left and right flank and behind the uterus ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Immunology 2024Quote: ... MASP-1/-3/MAP-1 mab 8B3 or F(ab’)2 fragments generated from an irrelevant mouse IgG1 mAb clone MOPC-31C (BD Biosciences, 557273) were coated on maxisorp plates at 4 °C ON and afterwards washed and blocked for 1 hr in TBS/T ...
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bioRxiv - Cancer Biology 2024Quote: CAR T-cells were incubated with target cells at an effector-to-target (E:T) ratio of 2:1 in complete RPMI 1640 medium containing GolgiStop™ Protein Transport Inhibitor Reagent (BD Bioscience, USA) and CD107a APC antibody (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed with flow cytometry buffer [PBS + 0.5% Bovine Serum Albumin (BSA) (Fisher BioReagents, BP9706100) + 2 mM EDTA] and incubated at 4°C with Fc Block (BD Biosciences, 553142) for 10 min ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Cell Biology 2023Quote: ... around 2×105 HEK001 cells expressed GFP (GFP+ HEK001) isolated by FACS Aria II cell sorter (BD Biosciences, San Jose, CA, USA) and cultured in one well of a 24-well plate ...
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bioRxiv - Microbiology 2023Quote: ... Blood was collected via cardiac puncture using a 1mL 28G1/2 insulin syringe and added to a serum collection tube (BD Microtainer 365967). Tubes were incubated for 45 minutes at room temperature to facilitate blood coagulation and then spun at 4 °C for 10 minutes (1500 x g) ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ produced upon re-activation of peptide-specific T cells and captured on anti-IFNγ capture antibody was visualized via sequential addition of 2 μg/mL biotinylated anti-IFNy detection antibody (BD #51-1818KA), Streptavidin-Horseradish Peroxidase (HRP ...
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bioRxiv - Immunology 2023Quote: ... and plated in round-bottom 96-well plates at 1-2 x 106 cells per well in complete RPMI (cRPMI) medium containing CD28 costimulatory antibody (BD Biosciences #553294) and brefeldin A ...
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bioRxiv - Biochemistry 2023Quote: ... Cells with the highest 2-5% GFP signal were selected two to three days post transfection using a flow cytometer (BD FACSAria II). Single cells were cultured in RPMI with 10% FBS ...
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bioRxiv - Systems Biology 2023Quote: ... all strains are grown in YPD (2% w/v Bacto Peptone, BD Biosciences #211677; 2% w/v D-glucose, BD Biosciences #215520; 1% w/v Yeast Extract, BD Biosciences #212750) at 30°C.
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bioRxiv - Synthetic Biology 2023Quote: ... 2% dextrose) when containing no plasmids and in synthetic defined (SD) media (0.67% yeast nitrogen base without amino acids [BD Difco], 2% dextrose) supplemented with the appropriate yeast synthetic drop-out medium supplement when plasmid maintenance was required (SDΔW-without tryptophan ...
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bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with PBS (2%FBS) and resuspended in 100ul ice-cold BD Phosflow™ Perm Buffer III (BD biosciences, USA) and incubated on ice for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... They were stained for 2 hours at room temp with the following primary antibodies: mouse anti-GM130 (1:250, BD Bioscience, BDB610822) and rabbit anti-TGN46 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-GFP (“Living Colors A.v. Peptide Antibody”) and mouse anti-UBE2E1 (BD Biosciences Inc.); rabbit-anti-γ-tubulin and mouse anti-β-actin clone AC-15 (Abcam Ltd.) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in sorting buffer (2% FBS and 1% Penicillin-Streptomycin in DMEM) and blocked with human FcR blocking reagent (Cat#564220; BD; used 1:50) for 10 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... cell suspensions were resuspended in PBS containing 2% FBS and then separated on a FACSAria Fusion flow cytometer (BD Biosciences, San Jose, CA). For single cell sorting ...
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bioRxiv - Cancer Biology 2019Quote: ... of both preparations was determined by titration onto the target cell line and quantification of the percentage of BFP positive cells 2-3 days post-transduction by flow cytometry (BD Biosciences LSR II).
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bioRxiv - Cell Biology 2019Quote: ... for 2 hours at room temperature followed by overnight incubation at 4°C with anti-PECAM-1 (MEC13.3, BD Bioscience #550274, 1:250) diluted in blocking buffer ...
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bioRxiv - Genomics 2021Quote: ... anti-CD3 (SP34-2: Alexa700; BD Biosciences, Custom Bulk 624040, PerCP-Cy5.5; BD Biosciences, Custom Bulk 624060, and Pacific Blue; BD Biosciences, Custom Bulk 624034), anti-CD4 (L200 ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed and resuspended in PBS supplemented with 2% FCS for FACS analysis (FACSCanto II; BD Bioscience, San Jose, USA). FACS data analysis was performed using BD FACSDivaTM Software 8 (BD Biosciences ...