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Citations for Becton, Dickinson and Company :
1051 - 1100 of 8593 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... anti-MASP-2 mAb F3-5 (Peter et al. 2022) and an irrelevant mouse IgG1 mAb (BD Biosciences, 557273) were coupled to to Dynabeads M-280 streptavidin (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... 5 x104 – 2 x105 leukocytes were examined per sample with a FACSCanto II (BD Biosciences, Franklin Lakes, NJ, USA), according to established multi-color protocols (Cossarizza et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mM EDTA in 1X PBS) and filtered through a 5 ml cell-strainer capped FACS tube (BD Falcon). A BD Biosciences FACSAria Fusion was used to sort tdTomato negative or tdTomato positive cells for gene expression analysis ...
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bioRxiv - Immunology 2021Quote: ... NKG2D-BV650 (1:40; clone 1D11; 563408) and γδTCR-APC (1:5; clone B1; 555718) from BD Biosciences.
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bioRxiv - Microbiology 2024Quote: ... Serum was diluted between 1:5 and 1:20 and IFN-γ levels were assayed by BD Mouse IFN-γ Flex Set Cytometric Bead Array according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: 2 × 106 LAN-1 cells in 200 μL PBS:matrigel (BD, 354248) (1:1 ...
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bioRxiv - Immunology 2023Quote: ... BV786 Rat Anti-Mouse CD138 (Syndecan-1) (Clone 281-2; BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Biotin Rat Anti-Mouse CD138 (Syndecan-1) (Clone 281-2; BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... anti-IgD (BD Biosciences, clone IA6-2, APC-H7, 1:50), biotinylated SARS-CoV-2 (JN.1 ...
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bioRxiv - Immunology 2023Quote: ... anti-BCL-2 PE-Cy7(1:200, clone 10C4, BD Biosciences) and anti-MCL-1 A647 (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... 2) Rabbit anti-mouse CD31(1:2000) (Cat. No. ab8216, BD Biosciences ...
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bioRxiv - Bioengineering 2020Quote: ... Tumor xenografts were inoculated subcutaneously on both flanks with 2.3 × 105 cells (MDA-MB-231) in a final volume of 100?μL of 1:1 DMEM and matrigel (BD). Tumor growth was routinely monitored using calipers to ensure the tumor weight not exceeding 10% of body weight ...
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bioRxiv - Cancer Biology 2020Quote: ... 1×106 BxPC-3 or PANC-1 cells were suspended in 40 μL mixed cell culture media and Matrigel (BD) at a 1:1 ratio and injected into the tail of the pancreas ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were dissected and dehydrated next day and were washed 3 times in PDT buffer (0.3 Triton-X in PBST with 1% DMSO) and incubated with Caspase-3 antibody (1:500; BD Biosciences) at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse anti-Calnexin99A (1:10, DSHB, Cnx99A 6-2-1) and rabbit-anti RFP(1:200, BD Bioscience, and 1:200, MBL), rabbit anti-Ref(2)P (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... were resuspended in defRPMI-1640 with 3% newborn calf serum and Benzonase™ (FACS buffer) in 5 mL FACS tubes and pre-incubated with GolgiStop™ (BD Biosciences) for ∼60 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 × 106 HCC cells were injected in a 1:1 ratio with Matrigel® (BD Biosciences). HCC patient-derived specimens were transplanted subcutaneously ...
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bioRxiv - Cell Biology 2023Quote: ... and homogenized by passing through a 20 G x 1-1/2 inch hypodermic needle (BD). The nucleic-acid-containing extracts (500 μl ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-caspase-3-phycoerythrin antibody (Clone#C92-605, 1:25) (Cat#550821, BD Pharmingen).
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bioRxiv - Cancer Biology 2024Quote: Mouse lung tissue was fixed in a 1:3 dilution of Cytofix fixation buffer (BD) in PBS for 18 hours ...
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bioRxiv - Bioengineering 2024Quote: ... or anti-human/mouse phospho-SMAD2/3-AF647 (clone 072-670, BD Biosciences, 1:100) in antibody staining buffer (1X PBS ...
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bioRxiv - Bioengineering 2020Quote: ... at 5:1:1 concentration ratio before transferred into a 1 ml syringe with BD Luer-Lok (BD, 309628, NJ, USA). The syringe was then hanged vertically to allow cell settling by gravity for 10-15 min with no flow ...
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bioRxiv - Microbiology 2022Quote: ... were grown in in Pro99 media supplemented with 5 g L-1 peptone and 1 g L-1 yeast extract (BD Difco). All heterotrophs were grown at 24 °C ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Immunology 2020Quote: ... up to 5×106 leukocytes were incubated with 5 μg/mL E6 peptide or 1 μg/mL α-CD3e (clone 145-2C11, BD Biosciences) in the presence of 1:1000 Golgi-plug for 5 hours ...
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bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 × g cells were carefully resuspended in annexin V staining buffer (RPMI1640 without phenol red containing 1 % FBS, 2 µg/ml lysostaphin, 2 mM CaCl2, 10 µl/ml annexin V-APC [BD Biosciences] and 10 µl/ml 7AAD [BD Biosciences]) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were allowed to recover for 2 days in 6-well plates filled with 4 ml medium per well before being FACS sorted for mCherry-expression on a BD FACS Aria III (BD Biosciences, Heidelberg, Germany). For subsequent limiting dilution cloning ...
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bioRxiv - Immunology 2021Quote: Control and SC-macrophages were fixed with 4% PFA for 10 mins and permeabilized with 1x perm wash buffer (BD cat#51-2091KZ) for 30 mins at RT ...
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bioRxiv - Immunology 2022Quote: ... were added to the ELISpot plate containing an immobilized primary antibody to either IFN-g or IL-4 (BD Cat# 551881 and BD Cat# 551878, respectively), and were exposed to various stimuli (e.g ...
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bioRxiv - Microbiology 2021Quote: ... a 10 µl inoculating loop was used to streak cecal slurry (cecal contents in BPW) onto Xylose Lysine Tergitol-4 agar (BD Difco, Sparks, MD). supplemented with 4 ppm tetracycline for trial 1 and BGS supplemented 32 ppm ampicillin (amp ...
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bioRxiv - Immunology 2020Quote: ... Pellets were then stained with a panel of antibodies for 30 minutes (4°C) followed by washes in PBS before analysis by flow cytometry (BD, Franklin Lakes, NJ).
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bioRxiv - Systems Biology 2021Quote: ... HeLa and RAW 264.7 cells were washed by chilled PBS and sorted on the nanoPOTS chips (4 × 12, 1.2 mm diameter per well) using the Influx II cell sorter (BD Biosciences, San Jose, CA) as described previously 14 ...
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bioRxiv - Immunology 2020Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... overnight and then stimulated with 1 nM N4 in the presence of vehicle or Compound 182 (1 μM) for 4 h at 37°C in complete T cell media supplemented with BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were then stained with fluorophore-conjugated antibodies to cell surface CD8 and intracellular IFNγ ...
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bioRxiv - Immunology 2023Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Microbiology 2022Quote: ... the individual co-cultures were 10-fold serially diluted with 1X anaerobic phosphate buffer saline (PBS) and plated on Xylose Lysine Tergitol 4 (XLT4) agar (BD Difco, Houston, TX). The plates were incubated aerobically at 37°C for 24 hours followed by plating on XLT4 agar and colony forming units (CFU ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Genomics 2023Quote: ... and half of the remaining chromatin was rotated overnight at 4°C with 4.5 µg of anti-p53 (BD Biosciences #554293, Lot #9046678). The other half of the remaining chromatin was also rotated overnight as a no-antibody control ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... mice were euthanized and tumors were quickly harvested and fixed for 14-16 hr at 4&C in BD Cytofix/Cytoperm (BD Bioscience, Cat #: 554722) diluted 1:4 in PBS ...
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bioRxiv - Immunology 2022Quote: ... In-between incubation steps, cells were washed (300 x g, 10 min, 4°C) with 40 mL of BD Cell Wash (BD Biosciences, Allschwil, Switzerland). In order to block Fc receptors ...
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bioRxiv - Microbiology 2022Quote: ... Afterwards the samples were fixated and permeabilized for 20 min and 60 min at 4 °C in the dark using BD Cytofix/Cytoperm™ solution and BD Perm/Wash™ solution (BD Biosciences), respectively ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Immunology 2023Quote: ... or anti-mouse IgA (BD Biosciences; C10-3; at 3 μg/ml) in PBS overnight and then washed three times with PBS and blocked with Tris-buffered saline containing 1% BSA (TBS/BSA ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were incubated on a cold block for 2-5 minutes before being plated in a 96 (12 x 8) grid on Omnitrays containing LB (BD, Franklin Lakes ...
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bioRxiv - Biophysics 2021Quote: ... 1 mL plastic syringes (BD PlastipakTM; sterile needles, 25G x 1” – NR. 18, 0.5 mm x 25 mm, BD MicrolanceTM 3), and portex tubing PE (Scientific Laboratory Supplies ...