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Citations for Becton, Dickinson and Company :
951 - 1000 of 8593 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Small intestine lamina propria cells from male C57BL/6J mice were prepared and stained with surface molecules for 30 min at 4 followed by sorting on a FACS Aria III cell sorter (BD Biosciences). A gating strategy of live Lineage−CD127+KLRG1-c-Kit+ was used to sort the ILC3s.
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bioRxiv - Immunology 2024Quote: Live single CD45+TCRβ+CD4+IL10+ cells were sorted from isolated lamina propria cell suspension from three pooled Giardia-infected mice per sample (total of 4 samples) using a BD FACSAria Cell Sorter (BD Biosciences). Then ...
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bioRxiv - Immunology 2023Quote: ... according to manufacturer instructions and stored at 4°C protected from light before acquisition on a FACSCalibur cytometer equipped with CellQuest Pro software (BD Biosciences) within 1 week of fixation ...
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bioRxiv - Immunology 2024Quote: ... surface antibodies were incubated with cells for 30 minutes at 4°C in hFACS buffer and Brilliant Stain Buffer (BD Biosciences). For intracellular staining ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were fixed in 4% PFA for 15 minutes at room temperature and permeabilised in Perm III buffer (BD Bioscience) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were incubated overnight at 4°C in the dark and sample analysis performed the following day using an LSR FortessaTM X-20 device (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: Ferrets were sedated with 4% isoflurane and blood was collected via the jugular vein into a heparinized collection tube (BD 366643). Plasma was separated via centrifugation and stored at –80°C until use ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with staining buffer, fixed, and permeabilized (20 min, 4°C, dark) using Fixation and Permeabilization Solution (BD Biosciences). Cells were then stained with S100A9 (Clone ...
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bioRxiv - Neuroscience 2022Quote: SH-SY5Y cells were seeded at a density of 4×103 cells/well in flat bottom 96-well plates previously coated with Matrigel (BD, Biosciences), and 10 μM all-trans-retinoic acid (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... stained on ice for 30’ with relevant cocktails of antibodies and populations of interest were sorted at 4°C on an BD FACSARIA (BD Bioscience) using a 70 μM nozzle in 50% FBS RPMI ...
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bioRxiv - Immunology 2022Quote: ... intracellular cytokine staining was performed at 4°C for 30 minutes using a cocktail of antibodies resuspended in 1x Perm/Wash buffer (BD Biosciences); intracellular antibodies included IFNγ APC-Cy7 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... cells were detached using 150 μl of PBS + 4 mM EDTA and analyzed via flow cytometry on a BD LSRFortessa Cell Analyzer (BD Biosciences). Transgene expression was detected in the PacBlue channel.
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bioRxiv - Immunology 2022Quote: ... 20 μm paraformaldehyde fixed lung sections from Mtb-mCherry infected I-Tomcat Ai6 and Sp140−/− I-Tomcat Ai6 mice were stained at 4°C overnight with BV421-labeled SIRP□ (P84, BD Biosciences), Pacific Blue–labeled B220 (RA3-6B2 ...
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bioRxiv - Cell Biology 2023Quote: ... 10-μM bromodeoxyuridine (BrdU) solution was added 4 h before the end of the experiment [fluorescein isothiocyanate (FITC) BrdU Flow Kit (BD #559619)] according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... They were finally washed with 4 mL FACS buffer and re-suspended in 0.5 mL to be analyzed in a LSR Fortessa X20 (BD Biosciences, Europe). For live staining ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: Stained cells were stored for up to 2 days at 4°C in the dark (TRAIL-R1) and flow cytometry was performed on a BD FACSCanto II (BD Biosciences). Raw data were exported to FlowJo v10.8.1 for analysis and plotting.
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence intensity of Mag-fluo-4-loaded cells was measured using a BD Accuri C6 flow cytometer (BD Biosciences, CA, USA) with an FL1 detector for green fluorescence and logarithmic amplification ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with a mouse pan α-synuclein primary antibody (Syn1, BD Biosciences, NJ, USA; #610786) diluted 1:5,000 in blocking solution ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... and stained for 30 min at 4 °C with the following antibody mixture: PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Fixed cells were stained for 30 min at 4° C with PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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bioRxiv - Genomics 2024Quote: ... The cells were then washed twice with FACS buffer and then analyzed for DAPI intentsity in LSR-Fortessa 4-9 HTS bench top flow cytometer (BD Biosciences). DAPI intensity for at least 10,000 individual cells were recorded for each sample.
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and fixed/permeabilized for 20 min at 4°C using the Cytofix/Cytoperm Fixation/Permeabilization Kit (BD Biosciences). Cells were washed with 1X PermBuffer (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... surface stained cells were fixed and permeabilized (20 min at 4°C) using Fixation/Permeabilization Solution before washes in Perm/Wash buffer (both BD Biosciences). Cells were then stained with intracellular Abs as above except in Perm/Wash buffer ...
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bioRxiv - Immunology 2024Quote: ... cell suspensions were incubated at 4°C or 37°C for 90 minutes and labelled with Annexin V-FITC or Annexin V-BV711 (BD Biosciences) in line with the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... #DY413 and #DY404) for IL-4 and IL-13 and BD™ Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD, #552364) for IL-2 ...
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bioRxiv - Bioengineering 2024Quote: ... washed and stored at 4 °C for analysis the next day on a on a flow cytometry instrument (LSR Fortessa, BD Biosciences). Flow cytometry buffer was used to prepare Fc block and antibody solutions ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained with Fixable Viability Dye (20 min, 4°C, Fixable LiveDead, eBioscience) and then with a mix containing a brilliant stain buffer (BD Biosciences) and the surface markers for CD4+ T cells detection (CD3 and CD4 ...
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bioRxiv - Immunology 2024Quote: ... The samples were then stained with the fluorophore-conjugated antibodies or isotype controls in a flow cytometry buffer for 30 minutes at 4 °C in the dark and analyzed with the FACS symphony A1 (BD Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... A human EC model of obesity and diabetes was established by growing HUVECs to confluency on gelatin-coated 24-well TC plates or glass chamber slides (4-well or 8-well; BD Falcon) in EGM-2 and thereafter changing to growth factor-depleted (GFD ...
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bioRxiv - Physiology 2024Quote: ... antibodies (or isotypic controls) for 30 min at 4°C before analysis was run using a FACSCanto II flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2024Quote: ... plates were washed 4 times with PBST and filled each well with 100 µl 1000 times diluted HRP-conjugated streptavidin (BD, 554066) in 0.5% skim milk PBST buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were incubated for 4 h and washed twice with PBS for analysis of fluorescein positive cells via flow cytometer (BD FACSCantoTM II, BD Biosciences). To further investigate the internalization mechanism of PoLixNano ...
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bioRxiv - Cell Biology 2024Quote: ... iECs were dissociated with TrypLE Express and fixed with 4% paraformaldehyde with 0.2% Triton X-100 and then the cells were filtered with 70 μm cell strainers (BD Falcon, 38030) in 1% BSA-PBS ...
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bioRxiv - Bioengineering 2024Quote: ... the frozen aliquot was thawed in a 37°C water bath for 4 min before being diluted in 35 mL stain buffer (BD, 554656) and pelleted at 300g for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Antibody staining was performed at 4 °C for 30 min and the stained cells were analyzed on an LSRFortessa cell analyzer (BD Biosciences). FACS sorting routinely yielded cell purity levels of over 90 % ...
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bioRxiv - Cancer Biology 2024Quote: ... 4:1 CTL:cancer cell admixtures were incubated for 4 h at 37°C in the presence of BD GolgiPlug protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... the E13.5 dams were injected intraperitoneally with 200 μl of 5-bromo-2’-deoxyuridine (BrDU) (552598, BD Pharmingen) early in the morning and the embryonic kidneys were collected 2.5 hours later ...
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bioRxiv - Genomics 2021Quote: ... supplemented with 10% modified ADC (2% glucose, 5% BSA, 0·85% NaCl) or in Middlebrook 7H9broth (BD Biosciences) supplemented with 10% modified ADC along with 0.05% Tween 80 without shaking.
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bioRxiv - Immunology 2021Quote: ... then washed and resuspended in PBS-2% FBS for flow acquisition on a 5-laser LSRII (BD BioSciences). For experiments with intranuclear transcription factor staining ...
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bioRxiv - Developmental Biology 2023Quote: 5-ethynyl-2’-deoxyuridine (EdU) staining was performed using the 647 EdU Click Proliferation Kit (BD Biosciences, 565456). First ...
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bioRxiv - Immunology 2021Quote: 1-2×106 PBMC were stained with the following surface antibodies (2 panels) against: CD3 (BD Biosciences,SP34), CD20 (Biolegend ...
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bioRxiv - Immunology 2021Quote: 1-2×106 BAL cells were stained with the following surface antibodies (2 panels) against: CD206 (BD, 19.2), CD169 (Biolegend ...
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bioRxiv - Physiology 2021Quote: ... and COX-2 (anti-COX-2: cat#: 610204, source: mouse, 1:1000; BD Biosciences, San Jose, CA, USA). The immunostaining was detected using horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG ...
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bioRxiv - Microbiology 2024Quote: ... auris strains were routinely grown on YPD medium (1% yeast extract, 2% peptone, and 2% glucose (BD Biosciences) at 30°C with or without shaking at 200 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5% BSA prior to 1 hour incubation in mouse anti-IdU (1:5, 347580, BD Bioscience) and rat anti-CldU-FITC (1:25 ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used were AlexaFluor® 647-conjugated anti-NKX6-1 (1:5 dilution, BD Biosciences 563338); PE-conjugated anti-insulin (1:50 dilution ...
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bioRxiv - Genomics 2020Quote: ... covered with or without 50 µl of Matrigel (1:3 dilution, BD, NJ, USA). Cells were then harvested and dissociated into a single-cell suspension ...
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bioRxiv - Bioengineering 2022Quote: ... acid solubilized Type 1 collagen from rat tail tendon (3 mg/mL, BD Biosciences) was reconstituted in an ice bath to a final concentration of 2.5 mg/mL ...
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bioRxiv - Immunology 2021Quote: ... Samples from Figures 1–3 were stained and collected with a LSRFortessa cytometer (BD) as previously described20 ...