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Citations for Becton, Dickinson and Company :
1001 - 1050 of 8593 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and mouse anti-BrdU (Cat#: 347580, BD Biosciences, 1:2.5 in 3% BSA/PBSTx). Secondary antibodies conjugated to AF488 ...
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bioRxiv - Molecular Biology 2024Quote: ... and subsequently for an antibody against active-caspase-3 (1:500, 559565, BD Biosciences). TUNEL labeling was performed first and according to the manufacturers’ instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... 3 g L-1 peptone (catalog number 211677, BD, Franklin Lakes, New Jersey, USA); 25 g L-1 mannitol (catalog number BDH9248 ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used for immunofluorescence: anti-CD63 (MBL, D263-3, 1:500; BD Biosciences, 556019, 1:1000), anti-LAMP1 (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were stained for 30 min on ice with 5 μl of biotin-labeled anti–cleaved caspase-3 monoclonal antibody (BD Biosciences, #550821). The cells were washed in Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... maintained for 20 min on ice and then homogenized with syringe every 5 min (BD Microlance™ 3 Needle 0,5 × 25 mm). Nuclear lysate supernatants were collected at 1000 rpm for 10 min at 4°C 43.
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bioRxiv - Neuroscience 2024Quote: ... Tracheal intubation was performed after sevoflurane exposure (5%, 3 minutes) using a 20-gauge intravenous catheter (REF 382434; BD Angiocath Plus, Singapore). To ensure proper positioning of the catheter so as to avoid one- lung ventilation and gas leakage during ventilation ...
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bioRxiv - Microbiology 2020Quote: ... 3 (BD Biosciences), 0.5 g/l soluble starch (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... 3 (BD Biosciences), 5 g of Na2HPO4 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Final cell pellets were resuspended at a cell density of 2×106 cells/ml in 3% FBS in PBS and analyzed at the UTSW Flow Cytometry Core Facility (FACSCalibur, BD Biosciences). FlowJo software was used to analyze mean fluorescent intensity (RRID:SCR_008520).
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bioRxiv - Biophysics 2021Quote: ... filled with motility buffer containing various concentrations of α-methyl-aspartate (MeAsp) through polyethylene tubing (Polythene Tubing, 0.58 mm id, 0.96 mm od; BD Intermedic). The tubing was connected to the PMDS device through stainless steel pins that were directly plugged into the inlets or outlet of the device (New England Tubing) ...
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bioRxiv - Developmental Biology 2024Quote: All cell culture of all species was maintained at 37°C with 5% CO2 and 5% O2 on Matrigel (1:100, BD Corning) coated plates unless otherwise specified ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μl of this suspension (1×10e5) was transferred to a 5 ml culture tube and 5 μl of FITC Annexin V (BD Biosciences) added ...
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bioRxiv - Immunology 2021Quote: ... Slides were treated for 10 minutes in H2O2 and after blocking in 10% goat serum in 0.1% Tween20 for 30 minutes and incubated overnight at 4°C with specific antibody diluted in PBS 0,1%Tween20: anti-mouse Ly6G (1A8, BD Pharmingen, cat 551459), or anti-mouse Ki67 (D3B5 ...
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bioRxiv - Bioengineering 2020Quote: ... was applied for 30 minutes at 4°C and stained cells were resuspended in FACS buffer before flow analysis on a BD FACSCanto™ II (BD Biosciences). Gating and flow data were processed on FlowJo (FlowJo LLC).
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bioRxiv - Microbiology 2021Quote: ... and several ten-fold dilutions (10−4 to 10−7) were plated onto de Man Rogosa and Sharpe (MRS) media (BD Difco, USA) supplemented with 50 mg/L of mupirocin (PanReac AppliChem ...
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Molecular Biology 2020Quote: ... Then the syndecan-4 expression on HUVECs was measured by flow cytometry with a BD Biosciences LSR II (BD Biosciences, CA, USA).
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bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
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bioRxiv - Cancer Biology 2020Quote: Plasma serum was collected from 4-6 drops of peripheral blood obtained from NSGS mice via submandibular bleeding using Microtainer Collection Tubes (BD Microtainer, # 365967). Concentrations of 29 cytokines/chemokines were measured in 2-4 biological replicates using MILLIPLEX Map Human Cytokine/Chemokine Magnetic Bead Panel (HCYTMAG-60K-PX29 ...
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bioRxiv - Neuroscience 2021Quote: ... A 1ml astrocyte cell suspension of 4×104 cells/ml was plated in three chambers of four-chamber glass slides (BD Falcon, USA) coated with poly-D-lysine ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2020Quote: ... in house production), 10 μg/mL α-IL-4 (cl. 11B11, in house production) and 10 ng/mL IL-12 (BD Pharmingen) and cultured on goat α-hamster IgG (MP Biochemicals ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cells were washed once in FACS buffer before fixation for 20 min at 4°C using the BD Cytofix/Cytoperm solution and permeabilization using BD Perm/Wash™ buffer (BD Biosciences). Permeabilized cells were incubated for 30 min with antibodies (diluted 1:200 in BD Perm/Wash ...
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bioRxiv - Genomics 2023Quote: Whole blood aliquots at a volume of four (4) milliliters were collected from participants using sterile Vacutainer blood collection kits (BD Vacutainer®) and subsequently biobanked at −80°C for DNA extraction ...
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bioRxiv - Immunology 2023Quote: ... Blood was collected via submandibular bleeds with goldenrod 4 mm lancets collected into sample tubes coated with K2 EDTA (BD Ref# 365974) and 60 μL of blood was lysed with 500 uL of ACK lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed at 4 °C for 20 min and washed in permeabilization buffer from the Fix/Perm kit (BD Biosciences, 554714). Antibodies against intracellular targets were diluted in permeabilization buffer and cells were incubated at 4 °C for 30 min followed by another wash prior to assessment with BD LSR Fortessa SORP cytometer ...
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bioRxiv - Immunology 2022Quote: ... The Fc cell receptors were then blocked for 10 min at 4°C with an anti-CD16/CD32 antibody (10 μg/mL: BD Biosciences 553142) and the cells were resuspended in 25 μL of staining buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... treatment was performed at 100μg/ml concentration in dark at 4°C for an hour and analyzed by FACS Calibur flow cytometer and processed using Cell Quest software (BD Biosciences, USA).
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL medium containing 1 × 106 luciferase-expressing MCF10AER/vSrc cells (control or 4-OHT transformed, with or without NLS-catalase expression) were mixed with 75 μL Matrigel matrix (BD Bioscience, #356237) containing 10 μM 4-OHT and injected into the inguinal mammary fat pad of the female mice at 6 to 8 weeks of age ...
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Immunology 2024Quote: ... Surface staining was carried out for 30 minutes at 4°C with the following fluorochrome-labeled anti-mouse Abs: APC/Cy7-CD45 (BD Bioscience, #557659), PE/Cy5-B220 (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... followed by two washes with the permeabilization buffer before incubation with 4 μg/ml Alexa Fluor® 647 Mouse anti-Phospho-NF-κB p65 (pS529) (BD Biosciences) for 45 min.
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bioRxiv - Microbiology 2024Quote: Cytokine secretion by WT RAW 264.7 macrophages stimulated for 4 h with LPS was measured by ELISA using BD OptEIA™ Mouse IL-6 ELISA Kit (BD Biosciences), BD OptEIA™ Mouse TNF ELISA Kit (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... saturated at 4°C for 20 minutes in DPBS containing 10% HI FBS and incubated with anti- CD163 Alexa Fluor 647 (BD Pharmingen, #562669) antibody for 1 hour and 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... in the dark at 4°C for 20min and acquired on a BD Fortessa flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). Proliferation of in vitro differentiated Th2 cells was also assessed using the same protocol and data was acquired on BD FACSAria III (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Cels were fixe with 4% PFA and stained with Rb anti-HA for NDST1 (CST 3724S) and Ms anti-GM130 (BD biosciences 610823) for Golgi followed by secondary antibodies staining with anti-Rb-A550 and anti-Ms-A647 ...
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bioRxiv - Neuroscience 2024Quote: ... The Fc cell receptors were then blocked for 10 min at 4 °C with and anti-CD16/anti-CD32 antibodies (10 µg/mL: BD Biosciences 553142) and the cells were resuspended in 25 µL of staining buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were centrifuged for 4 minutes at 2,000 RPM and resuspended in 600 μL of cold BD Perm/Wash buffer (BD Biosciences, cat# 554723). Goat serum (30 μL ...
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Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... and AlexaFluor® 647-conjugated anti-NKX6.1 (1:5 dilution, BD Biosciences). Data were processed using FlowJo software v10.
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bioRxiv - Genomics 2020Quote: ... and AlexaFluor® 647-conjugated anti-NKX6.1 (1:5 dilution, BD Biosciences). Data were processed using FlowJo software v10.
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bioRxiv - Microbiology 2020Quote: ... CD4 clone RM4-5 conjugated with phycoerythrin (PE) (1:150; BD Biosciences), CD8 clone 53-6.7 conjugated with allophycocyanin (APC)-Cy7 (1:150 ...
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bioRxiv - Cell Biology 2020Quote: ... cell suspensions were incubated with CD45-FITC (555482, 1:5, BD Bioscience) and CD56-PE (555516 ...
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bioRxiv - Physiology 2022Quote: ... labeled with anti-CD4 antibody (1:1000; clone RM4-5; BD Biosciences) and fixed with 4% formaldehyde overnight as described (Ciucci et al. ...
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bioRxiv - Immunology 2024Quote: ... and anti-IL17 diluted 1/5 (SCPL1362, BD Bioscience, cat. no. 560436) were added ...
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bioRxiv - Immunology 2024Quote: ... anti-human IgM (FITC, clone: G20-127, BD, cat# 555782, 1:5), anti-human IgG (PE-Cy7 ...
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bioRxiv - Immunology 2022Quote: ... mice were injected ip with 200 µL of 10 mg/mL 5-bromo-2’-deoxyuridine (BrdU, BD cat. 559619). Mice were culled by cervical dislocation at the time point indicated in the individual experimental setup ...