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Citations for Becton, Dickinson and Company :
751 - 800 of 806 citations for Recombinant Cynomolgus CD22 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... resuspended in 50ul FACS buffer containing 0.5 μg anti-mouse CD16/CD32 mAb 2.4G2 (Mouse BD Fc Block, BD Pharmingen, San Diego, California), and incubated for 15 minutes 4° C ...
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bioRxiv - Immunology 2020Quote: ... and serum-blocked in 2.5% goat serum/PBS containing 1:100 dilution of Fc block (CAT # 553142; BD Biosciences, San Jose CA). For B ...
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bioRxiv - Cancer Biology 2021Quote: ... Reactions were terminated by addition of DMEM containing 8% FCS and cell suspensions were dispersed through a 70 μm cell strainer (BD Falcon, 352350). All single-cell suspensions were treated with RBC lysis buffer to remove red blood cells ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions from mouse tissues or tumors were incubated for 15 minutes at ambient room temperature with rat anti-mouse Fc Block™ (BD Pharmingen). Cells were then incubated with primary antibodies for 30 minutes at 4ºC in the dark ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
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bioRxiv - Genetics 2022Quote: ... and ICAM1-Fc (a gift from Professor Alister Craig, Liverpool School of Tropical Medicine) spotted on bacteriological petri dishes (BD Falcon 351007) at a concentration of 50μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cells were washed twice with FC buffer by centrifugation (1000 rpm/5minutes) and incubated with streptavidin−PE conjugate (BD bioscience, 349023) for 30 minutes at 4ºC ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed with flow cytometry buffer [PBS + 0.5% Bovine Serum Albumin (BSA) (Fisher BioReagents, BP9706100) + 2 mM EDTA] and incubated at 4°C with Fc Block (BD Biosciences, 553142) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... 5X106 cells per mL were washed with cold PBS and blocked in PBS with Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Biosciences) for 15 min at 4°C in the dark ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 μL of PBS containing 5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Eluent was washed twice, incubated with DR (Bio-Legend, 424101, 1:1000) and CD16/CD32 Fc block (BD Biosciences, 553141, 1:200) at room temperature for 15 minutes ...
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were counted using a hemacytometer and then treated with an Fc blocking buffer containing anti-Cd16/32 (BD Biosciences, 1:500) for 20 minutes at 4 degrees Celsius before a 30 minute incubation with antibody master mixes for the myeloid or lymphoid panels at 4 degrees Celsius ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 and MIP-2 were measured using paired antibodies and recombinant cytokines according to the manufacturer’s recommendations (BD PharMingen Systems) (11).
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bioRxiv - Genetics 2020Quote: ... cells were stained with recombinant biotinylated spike protein RBD as described above and analyzed with multiparametric flow cytometry on a FACS Aria III (BD Biosciences) and FlowJo.v10 software (BD Biosciences).
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bioRxiv - Genetics 2020Quote: ... and the amounts of recombinant ACE2-GFP and dACE2-GFP proteins in lysates were quantified by densitometry analysis (Imagelab, BD Biosciences) of Western blots ...
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bioRxiv - Immunology 2022Quote: ... and 5e6 spleen cells were restimulated in vitro with 5e6 irradiated ERAAP KO spleen cells and 20 U/ml of recombinant human IL-2 (BD Biosciences). After 6 days ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for 30 min on ice with biotinylated recombinant HAs and PD-1-BB515 (EH12.1, BD Horizon, 1:100) diluted in P2 ...
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bioRxiv - Microbiology 2023Quote: ... and values for IL-2 were determined by using a standard curve generated by using known concentrations of purified recombinant murine IL-2 (BD Biosciences).
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed and resuspended in PBS supplemented with 2% FCS for FACS analysis (FACSCanto II; BD Bioscience, San Jose, USA). FACS data analysis was performed using BD FACSDivaTM Software 8 (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was prevented using the ells were stained using the BD Pharmingen Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Clone 2.4G2 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Immunology 2021Quote: We permeabilized formalin-fixed glomerular and tubulointerstitial single cells with 0.25% Tween-20 in PEB buffer at room temperature for 30 minutes and blocked cells with Fc Block (clone 2.4G2, BD Biosciences, San Jose, CA) diluted in 0.25% Tween-20 on ice for 10 minutes ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
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bioRxiv - Physiology 2021Quote: ... Following resuspension in PBS and passage through a 70μm strainer, cells were incubated in Fc block solution (Bio Rad, Cat# BUF041A) and PerCP-conjugated anti-CD11b antibody (BD Pharminogen, Cat# 550993). After PBS washes ...
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bioRxiv - Immunology 2021Quote: ... samples were stained with the relevant combination of surface and intracellular antibodies in combination with Fc blocking antibody CD16/CD32 (BD Pharmingen, Cat. #553141). BV421 anti-MHCII(IA/IE ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in x μL of unlabeled CD16/32 mAb Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), BD Pharmingen 553141) ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Physiology 2023Quote: ... then cells were washed with 1x PBS and resuspended in FACS buffer and non-specific binding to Fc receptors was blocked by incubation with anti-CD16/32 antibody (BD Pharmingen, Cat# 553141) and the FACS antibodies BV711 conjugated anti-CD11b and Pacific blue conjugated anti-CD45 antibodies (Cat# 101241 ...
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bioRxiv - Cancer Biology 2023Quote: ... 200 μL per 1x10e6 cells) with human IgG-Fc block (1:100) followed by the incubation with streptavidin-magnetic bead particles (BD Biosciences, Ref#557812) at 50 μL per 1x10e6 cells ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... cells were restimulated ex vivo for 4-5h at 37 °C in complete RPMI medium (RPMI supplemented with 2mM L-Glutamine and 10% fetal calf serum (FCS)) with GolgiPlug (BD, 1:1,000 dilution), phorbol 12-myristate 13-acetate (50ng/ml ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 30 min on ice with biotinylated and Alexa Fluor 647-conjugated recombinant soluble S and PD-1-BB515 (EH12.1, BD Horizon, 1:100) in 2% FBS and 2 mM EDTA in PBS (P2) ...
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bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Developmental Biology 2021Quote: ... were plated in buffer (PBS with 0.5% bovine serum albumin and 1 mM EDTA) and non-specific Fc binding was blocked via incubation with mouse anti-rat CD32 (BD Biosciences #550271; 1:200) for 30 minutes on ice ...
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bioRxiv - Immunology 2020Quote: The following rat anti-mouse antibodies were used for leukocyte analysis within each tissue: purified CD16/CD32 Fc blocking antibody (clone 2.4G2; BD Pharmingen™, San Jose, CA), PerCP-Cy™5.5 Ly6G antibody (clone 1A8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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bioRxiv - Cancer Biology 2023Quote: ... APC-eFluor780-conjugated cKit and BV605-conjugated Fc Gamma (CD16/CD32) for 30 minutes followed by fixation and permeabilization using BD Cytofix/Cytoperm (BD Biosciences, San Jose, California). Intracellular staining using FITC-conjugated KI-67 was performed overnight at 4 degrees ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Microbiology 2023Quote: ... spun for 10 minutes and then resuspended in 50 μL of a 1:200 dilution of Fc block (CD16/CD32, clone 2.4G2, BD Biosciences, cat. 553141, 0.5mg/mL) in FACS buffer (PBS ...