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Citations for Becton, Dickinson and Company :
501 - 550 of 806 citations for Recombinant Cynomolgus CD22 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CD4 (#560181), CD11b (#553312), 2.4G2 (Fc block, #553142) and Perm/Wash Buffer (#554723) were from BD Pharmingen (San Diego ...
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bioRxiv - Immunology 2023Quote: ... cells were spun down and resuspended in 50μL of human Fc block (BD Biosciences Cat. 564220) in DPBS and incubated for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... samples were blocked with Human BD Fc Block™ solution (1 µL/200,000 cells) (BD Biosciences) for 10 minutes at room temperature to reduce any non-specific antibody staining by Fc Receptors expressed on macrophages ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated in a blocking solution containing Human BD Fc Block (564220; BD Biosciences) diluted 1:25 (v/v ...
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bioRxiv - Cancer Biology 2022Quote: Immune cells were first incubated with Fc block (anti-CD16/CD32 1:100; BD Biosciences #553142) and then stained with one of two immunophenotyping panels diluted in 1x FACS buffer (2% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pre-incubated with mouse Fc blocker (BD 553142, 1 μg/million cells in 100μl) on ice for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with appropriate antibodies for 25 minutes on ice in the dark ...
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bioRxiv - Cancer Biology 2023Quote: Anti-CD16/CD32 Rat anti-mouse Fc Block (BD Pharmingen, #553142, 0.5 mg/mL, 1:400)
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bioRxiv - Neuroscience 2023Quote: ... and blocked for 15 minutes with 1:20 human FC block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE-Cy7 anti-human CD71 (TFRC ...
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bioRxiv - Cell Biology 2024Quote: Freshly thawed human and rhesus CD34+ cells were incubated with human Fc BlockTM (Becton Dickinson (BD), Franklin Lakes ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with fluorescent dye-labeled antibodies after incubation with Fc block reagent (BD Biosciences), and analyzed on the FACSCanto Ⅱ (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: MLN cell suspensions were incubated with Fc-block (anti-CD16/CD32, BD Biosciences, cat no. 553142) followed by staining with antibodies against surface and intranuclear markers ...
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bioRxiv - Cell Biology 2020Quote: ... RPE1 cells stably expressing YFP-tagged BBSome subunits were sorted using the 488 nm laser on FACSAria IIu (BD Biosciences).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Biophysics 2020Quote: ... Positive cells were amplified under antibiotic selection pressure and sorted for low or intermediate expression level of the fluorescently tagged protein on an Aria II Fluorescence Activated Cell Sorter (BD). Each sorted population was then used for pilot experiments to determine the lowest possible expression level required for optimal imaging conditions ...
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bioRxiv - Cell Biology 2021Quote: Expression of the various GPR116 constructs tagged with V5 or FLAG was verified by immunocytochemistry (Cytofix/Cytoperm, BD 51-2091KZ). Cells were plated in 8-chamber slides (Ibidi ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Immunology 2022Quote: ... Cell subsets for the populations from different donors were labelled simultaneously with the cell surface sorting panel (Table S4) an oligonucleotide-tagged antibody sample tag (BD Biosciences) prior to cell sorting on a FACSAria3 (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... left 24 hours before addition of drug and cell death was assessed following live cell staining with FITC-tagged AnnexinV (BD Biosciences) and addition of PI (Sigma ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were then tagged with propidium iodide and purified using flow-assisted cell sorting (FACS; BD FACS Aria II, 70 uM nozzle, BD Biosciences) and brought to a concentration of 1000 nuclei/uL ...
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bioRxiv - Immunology 2021Quote: The PBMCs were first resuspended in FACS buffer (PBS + 2% FBS) containing Fc blocking antibodies (BD, 564765) and incubated for 15 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... cells were spun down and resuspended in 50 μL of human Fc block (BD Biosciences Cat. 564220) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Microbiology 2021Quote: ... After pre-incubation of samples with purified rat anti-mouse CD16/CD32 antibody (Mouse BD Fc Block) (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were incubated for 10 min with rat anti-mouse CD16/CD32 Fc Block™ (BD Pharmingen) in Stain Buffer (BD Pharmingen ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then washed and resuspended in FC buffer before running on a LSRFortessa flow cytometer (BD).and anlayzed using FlowJo software version 10.
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bioRxiv - Microbiology 2021Quote: FC analysis of PI-stained bacteria was carried out using BD Accuri™ C6 device (BD Biosciences). Cell counts ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were incubated with anti-mouse CD16/32 antibody (Mouse BD Fc Block™, BD Pharmingen™). Cells were washed and incubated with the antibody staining mix ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were incubated with anti-mouse CD16/32 antibody (Mouse BD Fc Block™, BD Pharmingen™). Cells were washed and incubated with the antibody staining mix ...
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bioRxiv - Immunology 2019Quote: ... Cells were Fc-blocked with purified rat anti-mouse CD16/CD32 (Clone: 2.4 G2, Becton Dickinson BD) for 15 minutes at RT ...
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bioRxiv - Immunology 2019Quote: ... RRID:AB_2157728) diluted in 1% BSA with Fc block (Rat Anti-Mouse CD16/CD32, #553141, BD Bioscience, RRID:AB_394656) at the same dilution in PBS for overnight at 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cytoflex-generated FCS flow data files were analyzed using FlowJo 10 software (BD Biosciences, Ashland, OR, USA). The violet SSC-area by SSC-height data was gated for data analysis sets to exclude upper and lower extremes that would interfere with the calculation of the slope of the line ...
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bioRxiv - Physiology 2021Quote: ... Cells were Fc-blocked using Purified Rat Anti-Mouse CD16/CD32 (Clone 2.4G2, 553142, BD Pharmingen™) for 10 minutes and washed with FACS buffer (Ca2+ and Mg2+-free DPBS supplemented with 0.5% BSA and 0.3% sodium azide) ...
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bioRxiv - Immunology 2022Quote: ... and non-specific antibody (Ab) binding was inhibited by adding Fc Block (553142, clone 2.4G2; BD Pharmingen). Blocked cells were stained with a mixture of fluorochrome-conjugated Abs (see Online Supplemental Table 1 for list of Abs) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were incubated with blocking Purified Rat Anti-Mouse CD16/CD32 (553142, Mouse BD Fc Block™) for 15 min at 37°C before incubation with primary antibodies Alexa Fluor 647 Rat Anti-mouse CD31 (BD Pharmigen cat:563608 ...
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bioRxiv - Immunology 2020Quote: ... The single cell lung suspensions were incubated with CD16/32 (Fc Block BD Biosciences, San Jose, CA) to minimize nonspecific antibody staining ...
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bioRxiv - Immunology 2021Quote: ... and washed with PBS + 2% FBS and incubated with anti-mouse CD16/CD32 Fc Block antibody (BD), for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... before an additional centrifugation and resuspension in 1:400 CD16/CD32 (BD Fc BlockTM, BD Biosciences #553142) for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... before an additional centrifugation and resuspension in 1:400 CD16/CD32 (BD Fc BlockTM, BD Biosciences #553142) for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... the cells were first incubated for 10 min with human Fc block (#564220, BD Biosciences, CA, USA) in DPBS-10% FCS ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometry was done by BD.LSR Fortessa Cell analyser and analysis was done via FCS Express 5 software from BD (Beckton Dickinson).
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bioRxiv - Neuroscience 2023Quote: ... Unspecific staining was blocked using an anti-mouse CD16/CD32 Fc block (553142, BD Biosciences, 1:100) and DAPI (D1306 ...
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bioRxiv - Developmental Biology 2022Quote: ... These cells were incubated with Mouse BD Fc Block™ (Cat #553142, BD Biosciences, San Jose, CA) to reduce non-specific antibody staining caused by receptors for IgG ...
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bioRxiv - Physiology 2023Quote: ... Cells were incubated with a purified rat anti-mouse CD16/32 (clone 2.4G2, Fc Block, BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Biosciences, San Jose, CA, USA) was added at a concentration of 1 µg per 106 cells ...
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bioRxiv - Immunology 2023Quote: ... Concatenating fcs files in the same group into one file was conducted by FlowJo v10.8 (BD Biosciences). Manual gating ...
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bioRxiv - Immunology 2023Quote: ... and stained with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (#553142, BD Biosciences), Donkey anti-Goat IgG Alexa Fluor 488 (#A11055 ...
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bioRxiv - Cancer Biology 2023Quote: ... Nonspecific binding of antibodies to cell Fc receptors was blocked using 10 μl FcR blocking reagent (BD Purified Rat Anti-Mouse CD16/CD32 553142 ...
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bioRxiv - Biochemistry 2023Quote: ... pellets were resuspended in FACS buffer (PBS + 2% FCS) and analyzed by flow cytometry (BD LSRFortessa™) and FlowJo software.
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bioRxiv - Immunology 2023Quote: Single-cell suspensions were preincubated with mouse anti-Fc receptor antibodies (BD Biosciences, Franklin Lakes, NJ, USA), stained with antibodies for 30 min at 4[ ...