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Citations for Becton, Dickinson and Company :
551 - 600 of 806 citations for Recombinant Cynomolgus CD22 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Exploratory mass cytometry analysis reveals immunophenotypes of cancer treatment-related pneumonitisbioRxiv - Immunology 2023Quote: ... Concatenating fcs files in the same group into one file was conducted by FlowJo v10.8 (BD Biosciences). Cytobank Premium (Cytobank Inc. ...
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bioRxiv - Immunology 2024Quote: ... For spectral cytometry unmixed in SpectroFlo FCS 3.0 files exported and analyzed using FlowJo v10.9 Software (BD).
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bioRxiv - Immunology 2023Quote: ... Cells at a concentration of 5 million/ml were first incubated with human Fc block (BD Biosciences) on ice for 10 min ...
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bioRxiv - Immunology 2024Quote: ... treated with Fc block (anti-mouse clone 2.4G2) and stained with the following antibodies purchased from BD Biosciences or Invitrogen ...
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bioRxiv - Microbiology 2022Quote: In vitro CYP inhibition assay was performed using a standard commercial recombinant CYPs kit (BD-Gentest) as recommended by the company ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent antibody or recombinant protein biding on fluorescent nanobeads were directly counted and measured by BD FACSCelesta™ flow cytometer.
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bioRxiv - Cancer Biology 2021Quote: ... cells from digested tumors were incubated with Fc block (5 µg/mL; BD Biosciences, CA, USA; clone 2.4G2) and stained with antibody mix for 30 min and Viability Dye eFluor™ 780 (eBioscience ...
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bioRxiv - Pathology 2022Quote: ... Murine Fc receptors were blocked using anti-mouse CD16/CD32 (FcBlock clone 2.4G2, BD Biosciences, #553141, 1/200) and cells were stained in FACS buffer for 30 min at 4°C with the following anti-mouse antibodies ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were first incubated with mouse CD16/CD32 Fc–blocking antibodies (clone 2.4G2, BD Biosciences Cat# 553142) and Aqua LIVE/DEAD® Fixable Dead Cell Stain (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... in FACS buffer (PBS, 1% FCS, 0.02% Sodium Azide, 2mM EDTA) and 50μl of BD Brilliant stain buffer (BD) for 30 minutes on ice ...
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bioRxiv - Immunology 2019Quote: ... non-specific binding of immunoglobulin to Fc receptors was blocked by anti-mouse CD16/32 antibody (BD Biosciences). Cells were stained in FACS buffer (PBS without Ca2+/Mg2+ supplemented with 2% FBS and 2 mM EDTA ...
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bioRxiv - Immunology 2021Quote: ... and non-specific binding was blocked by adding 5% (v/v) normal donkey serum and Fc block (BD). Avidin was used to neutralize endogenous biotin ...
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bioRxiv - Microbiology 2021Quote: ... After pre-incubation of samples with purified rat anti-mouse CD16/CD32 antibody (Mouse BD Fc Block) (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... The remaining myelin-depleted cell suspension was blocked for ten minutes with Fc preblock (CD16/ CD32, BD 553141) on ice and stained for 20 minutes with antibodies to distinguish brain endothelial cells (CD31+/ CD45-) ...
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bioRxiv - Cancer Biology 2020Quote: ... myeloid and hematopoietic stem and progenitor antibody panels after blocking with anti human fc block (BD Pharmingen 564219) for 10 minutes at room temperature (Supplementary Table 7 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were blocked by adding 5µg/ml purified rat anti-mouse CD16/CD32 (mouse BD Fc Block) clone ...
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bioRxiv - Cancer Biology 2021Quote: ... The purification efficacy was analyzed by FC using the following surface markers: anti-CD45/PE antibody (BD Bioscience), anti-CD56/BB515 antibody (BD Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice in PBS + 2% FCS + 2 mM EDTA and incubated with mouse FcBlock (BD Biosciences), Epcam-APC (CD326 Monoclonal Antibody (G8.8) ...
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bioRxiv - Microbiology 2022Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Immunology 2019Quote: ... Following FACS sorting the individual blood and tissue-derived subsets were incubated with Fc block reagent (BD Biosciences) and Sample Tag antibodies for 20 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Without additional washing, an antibody cocktail containing Fc block (14-0161-86, eBioscience) BV buffer (566349, BD Bioscience) were added ...
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bioRxiv - Bioengineering 2019Quote: All antibody cocktails contained 1:100 dilution of anti-mouse Fc Receptor block CD16/CD32 (BD Biosciences 553141). Primary antibodies against H2-M3 was detected with secondary antibody conjugated to Alexa568 (Thermo Fisher A21112 ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were blocked for 10 minutes on ice with 1:100 diluted FC Block (BD Pharmingen, Clone 2.4G2) and metal-conjugated antibody cocktail added and incubated for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Surface antibody staining was done in PBS containing 1% FCS and 1X Brilliant Stain Buffer (BD Biosciences, USA) for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding and HIS48-FitC ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pre-incubated with anti-mouse CD16/32 mouse Fc blocker (BD Biosciences, San Jose, CA, USA) prior to staining with the antibodies ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated with Fc block and surface stained with anti-CD4 BUV805 (BD Biosciences; clone: RM4-5) or CD4 APC-Alexa Fluor 780 (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 15 min at 4°C with Fc-block (anti-CD16/CD32, 2.4G2, BD biosciences) before staining ...
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bioRxiv - Cell Biology 2021Quote: ... and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences, USA). Samples were analyzed by Guava® easyCyte™ Flow Cytometer (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: The following anti-mouse antibodies were used following manufacturer’s instructions: anti-CD16/CD32 Fc blocking antibody (BD Biosciences), anti-CD24-FITC (clone M1/69 ...
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bioRxiv - Immunology 2022Quote: ... and were resuspended in FC stain buffer prior to data collection via an LSR Fortessa flow cytometer (BD). Analysis was done using FlowJo software (version 10.7.1) ...
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bioRxiv - Immunology 2022Quote: For analysis of various thymus stromal cell populations human samples were blocked with human Fc-block (BD Bioscience) for 10 minutes at 4°C and then stained with Lineage cocktail-FITC ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked with 100 mL solution containing anti-mouse CD16/CD32 (1:100, BD Pharmingen, 553141) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions stained in PBS + 2% FBS in the presence of Fc receptor blocking antibody (BD Pharmingen) and stained with the antibodies against the following mouse proteins ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were sorted into HBSS-2% FCS in 15ml Falcon tubes using a BD Aria III (BD Biosciences). Monocytes were gated as Lineage-negative (B220 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding ...
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bioRxiv - Microbiology 2023Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Immunology 2023Quote: ... Aspecific epitope binding was blocked with 0.5 mg/mL human Fc Block (BD Biosciences, San Jose, CA, USA). Mononuclear cells were stained for extracellular epitopes for 1h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were Fc-blocked with anti-mouse CD16/CD32 (1:100, Cat# 553141, BD Biosciences, San Jose, CA) followed by surface marker staining with antibodies to MerTK (2B10C42 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with FACS buffer (PBS + 0.1% BSA) and incubated with Human Fc block (5µl per 2×106 cells; BD) at room temperature for 10 min ...
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bioRxiv - Genetics 2023Quote: ... 15 mins) and resuspended in 3mL DPBS supplemented with 30 µL Human BD Fc Block (BD Pharmingen, 564220). The sample was gently rocked at room temperature for 30 mins ...
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bioRxiv - Immunology 2023Quote: ... and incubated for another 15 minutes at 4°C in the presence of Fc receptor block (BD Biosciences). Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail ...
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bioRxiv - Cell Biology 2023Quote: FC analyses of SkM single cell suspensions were performed with the cell analyser LSRFortessa X-20 (BD Bioscience) using the FACSDiva 8.0 software ...
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bioRxiv - Cell Biology 2023Quote: ... Fc receptors were blocked with anti-CD16/32 in the antibody staining solutions (BD Biosciences, Franklin Lakes, NJ). For RPE and endothelial identification ...
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bioRxiv - Immunology 2023Quote: Cells were harvested and suspended in 50 μL of FCM buffer (0.5% BSA and 0.05% sodium azide in PBS) with 2.5-10 μg of Human BD Fc Block (BD). Cells were stained using the antibodies listed in Supplementary Table 1 for 15 minutes in the dark at room temperature and washed three times with FCM buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/ml human recombinant epidermal growth factor (hr-EGF; 354052, BD Pharmigen, San Jose, CA, USA), 0.4 mg/ml hydrocortisone (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
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bioRxiv - Cell Biology 2020Quote: ... Antibodies against CD16/32 (Fc block; clone 2.4G2) and ITGB2 (clone CTB104; Alexa Fluor 488 conjugated) were from BD Pharmingen and Santa Cruz Biotechnology Inc. ...
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bioRxiv - Immunology 2022Quote: Flow cytometry of peritoneal cells was performed using anti-mouse CD16/32 (Fc Block, BD Biosciences, San Jose, CA) before staining with primary antibody or isotype controls ...
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bioRxiv - Immunology 2019Quote: ... The cells were resuspended in FACS buffer (HBSS/ 1% FBS) then treated with Fc block (anti-mouse clone 2.4G2 and anti-human clone 3G8) purchased from BD and stained with specific antibodies ...