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Citations for Becton, Dickinson and Company :
6951 - 7000 of 7237 citations for Dectin 1 CLEC7A Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and either 1×106 VCaP or 5×105 22Rv1 cells suspended in 100 μl of PBS with 50% Matrigel (BD Biosciences) were implanted subcutaneously into the dorsal flank on both sides of the mice ...
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bioRxiv - Immunology 2023Quote: ... Single cells were then washed with 1% Bovine serum albumin (BSA) in PBS and blocked with anti-Fcγ receptor (BD Biosciences). Human peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells followed by an incubation with the SM/Chol probe OlyA (3 µM) and primary anti-IFN-γR1 antibody (1:50) (BD Biosciences) together in 1% BSA-PBS at room temperature for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... After washing the slides were blocked for 30 min in Superblock solution following the incubation with the primary antibodies mouse anti-BrdU/IdU (1:100, BD Bioscscience) and rat anti-BRDU/CIdU (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... and total DNA (7-AAD 1:50 solution dilution) and analyzed by flow cytometer in a BD LSRFortessa cell analyzer (BD Biosciences) using 488 nm and 640 nm lasers ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice in PBS-FBS 1% and stained with 20 µL of anti Ki-67 (FITC Mouse Anti-Ki-67 Set, BD Biosciences) for 30 min at RT in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Stained cells were analyzed and 7AAD−Lin−Vcam−Sca1+ (Stem cell antigen 1; Ly6a) (FAPs) were isolated using FACS Aria III cell sorter (BD Biosciences) with 4-way-purity precision ...
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bioRxiv - Immunology 2023Quote: ... Effector cells were incubated with K562 and Nalm6 variants for 4 hours at a ratio of 5:1 with addition of Brefeldin A (GolgiPlug, BD Biosciences). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... per well of U-bottom plate and coincubated for 4h at 37°C in the presence of NH4Cl and 1 µl Golgi PLUG (BD Biosciences). After that time cells were washed three times with 200 µl PBS and centrifuged (5 min ...
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bioRxiv - Immunology 2023Quote: ... Cell purity was determined by flow cytometry (see Figure S6A) using antibodies specific against CD20-FITC (1:100 345792, BD Biosciences), CD14-PerCP (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8/CD4 T cells ratio and activation was assessed with BD Multitest CD3/CD8/CD45/CD4 (1:20; BD Biosciences, #342317) and APC-CD69 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... CFSE-labelled cells were boosted with HIV-1-Gag or vaccine-specific peptide pools in the presence of Brefeldin A solution (BD, Biosciences) and Monensin solution (BD Biosciences) ...
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bioRxiv - Cell Biology 2024Quote: ... ESLAM HSCs defined as CD45+EPCR+CD48-CD150+ as previously described,1 were isolated using a Becton Dickinson Influx sorter (BD Biosciences). Cells were sorted directly into culture dishes containing media or into Eppendorfs containing FACS buffer.
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bioRxiv - Molecular Biology 2024Quote: ... unsorted, sorted, and depleted fractions, were labelled with mouse anti-ASGPR1-PE (1:20 dilution, to detect porcine hepatocytes) (BD Pharmigen), rat anti-OC2-2F8 ...
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bioRxiv - Neuroscience 2024Quote: ... The organs of corti were then dissected from the otic capsule and co-stained with anti-Ctbp2 antibodies (1:800; 612044, BD Transduction) and anti-myosin VI antibodies (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... Additional samples were stained and analyzed as above using the following modifications: the samples were blocked with FcR block (1:200 dilution; 553141, BD Biosciences) for 10 min at 4°C and incubated with anti-mouse CD11b-PE (1:50 dilution ...
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bioRxiv - Bioengineering 2024Quote: ... 3 ml of spiked blood mixed with BD BACTEC Plus Aerobic medium (BD, USA) (3:1 Blood:BD BACTEC Plus Aerobic medium) was gently placed over the density media ...
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bioRxiv - Immunology 2024Quote: Activation of signaling pathways downstream of pre-TCR was analyzed by western blotting of cells incubated with 10 µg/ml of anti-CD3ε (UCHT-1; BD Biosciences) or anti-pTα (K5G3 ...
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bioRxiv - Bioengineering 2024Quote: ... 25,000 irradiated HM-1-luc cells were mixed with 3 × 105 PBMCs per sample and seeded in a 96-well ELISPOT plate (BD Biosciences) that was pre-coated with IFN-γ capture antibody (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... positive for wild type heterologous SOSIP probes and negative for V2 apex epitope mutant probes were sorted at 1 cell per well into 96-well plates on a BD FACSAria II machine using BD FACSDiva software (BD Biosciences). RNA extraction ...
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bioRxiv - Microbiology 2024Quote: ... and then stained with a panel of immunophenotyping antibodies (clone, dilution, manufacturer) at room temperature for 30min: CD45 BUV395 (30-F11, 1:400, BD Biosciences), CD24 BV510 (M1/69 ...
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bioRxiv - Immunology 2024Quote: ... fluorescens J4 clinical isolates were grown for 1 day at 30°C in YM broth (BD Biosciences, San Diego, CA, USA). After gavages ...
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bioRxiv - Molecular Biology 2024Quote: ... The emission wavelength ratios of intracellular Ca2+-bound and unbound Indo-1-AM were analyzed on a LSRII flow cytometer with FACSDiva software (BD Biosciences).
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bioRxiv - Molecular Biology 2024Quote: ... were stimulated by incubation with 5 µg ml-1 anti-IgM F(ab’)₂ fragment antibody (115-006-020; Jackson Immuno Research) with or without 5 µg ml-1 anti-CD40 antibody (553787, BD Pharmingen), with 100 ng/ml MPL-A (Invivogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibody staining for Cxcr4 was performed for 20 mins on ice using 1:100 APC-conjugated Rat anti-Mouse CD184/Cxcr4 (BD Pharmingen) in FC buffer and cells were then washed twice in FC buffer and resuspended in 1 μg/ml DAPI in FC buffer at ∼1-1.5 x 1E7 cells/ml to label dead cells and run the suspension through a cell strainer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with the primary antibody solution (1:100 dilution; PDGFRA-APC, Thermo Fisher, cat#17-1401-81; SCA1-FITC, Thermo Fisher, 11-5981-82; CD45-BV450, BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... BSA was removed and the desired mouse primary antibody was added in Antibody solution (0.5%BSA/0.01% Triton X-100/PBS) at the indicated dilutions: Anti-β-Catenin Clone 14/Beta-Catenin (BD Biosciences, 1:1000), anti-Lamin A (Abcam ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each pool) and brefeldin A (1 μg/ml; BD Pharmingen, USA) at 37°C for 12-16 hours ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μL pre-cleared cell lysate was combined with 200 μL assay reagent containing 1 mM D-luciferin (BD Biosciences, product #556878), 3 mM adenosine triphosphate ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used for immunofluorescence of HeLa cells and/or primary neurons were: mouse anti-Ataxin-2 (BD Biosciences 611378, 1:250), mouse anti-beta-III-tubulin (Covance MMS-435P ...
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bioRxiv - Immunology 2022Quote: ... were picked from a selective plate and inoculated in 1 mL SD-UT medium [yeast nitrogen base-casamino acids (YNB-CAA) (BD Biosciences 223120) + 2% D-(+)-Glucose (Sigma G5767-500G ...
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bioRxiv - Microbiology 2019Quote: ... The cell pellet was then resuspended in 1 ml of T-R minimal medium and injected into the IsoCaRB system using a 3 ml syringe (BD Biosciences # 309657) and a 20-gauge needle (Sigma-Aldrich #Z192511).
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bioRxiv - Synthetic Biology 2019Quote: ... It was sterilized by autoclaving after filling with 2.5 L animal component free complex medium containing 20 g L-1 yeast extract (BD Biosciences, Heidelberg, Germany), 40 g L-1 peptone from soymeal (Applichem ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed with 1x Permeabilization buffer (eBioscience) and incubated for 15 min in FACS buffer before adding the primary MASH-1 antibody (BD Biosciences, 556604), 1:250 ...
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bioRxiv - Cell Biology 2019Quote: ... Hematopoietic cells were harvested from OP9-DL1 cocultures by forceful pipetting and stained on ice for one hour with the following antibodies: biotin-CD4 (1:2000; BD Pharmingen, 553045), biotin CD8a (1:2000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS and resuspended in 0.5 ml of PBS containing 1 μg/ml propidium iodide (PI) and 50 μg RNase and analyzed by flow cytometry (BD LSR-II) MCF-7 cells were exposed to 10 Gy γ radiation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pelleted and washed twice with 1% FBS in PBS and subjected to flow cytometry using a FACSMelody™ (BD Biosciences).
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bioRxiv - Immunology 2021Quote: Mouse splenocytes were cultured in RPMI-10 and stimulated with the S peptide pool at 1 µg/ml/peptide in the presence of GolgiStop™ and GolgiPlug™ (BD) for 6 hours ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Bioengineering 2021Quote: ... albicans fungal cells (CMM16) were inoculated from a streaked plate in 2 ml yeast extract peptone dextrose (YPD) [1% yeast extract (BD Biosciences, 212730), 2% peptone (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... was added after 1 hour and cells were stained for surface NK makers (CD56 and CD16) and intracellular IFN-γ (Cytofix/Cytoperm; BD Biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... 0.1% sodium azide) and re-suspended in 500µl of ice-cold FACS buffer prior to flow cytometric analysis (BD LSR II Fortessa). Data were analyzed using the FCS Express software.
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bioRxiv - Microbiology 2020Quote: ... Sections were washed and stained with primary antibody to E-cadherin (1:100 anti E-cadherin mouse monoclonal antibody, BD Biosciences 610181) and/or primary antibody to O157-antigen (1:2000 anti E ...
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bioRxiv - Microbiology 2021Quote: ... 2×106 lymph node cells were stained with the following monoclonal antibodies: rat anti-mouse CXCR5-biotin (clone 2G8, 1:100, BD, cat#551960), streptavidin PECy7 ...
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bioRxiv - Cancer Biology 2020Quote: ... then probed with the following primary antibodies in 5% milk/TBS-T overnight at 4 °C: SREBP-2 (1:250; BD Biosciences, 557037), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2022Quote: ... washed three times with RPMI and incubated for 30 mins at 37°C in RPMI containing Dispase (BD Cornig, 1:10 dilution) and 30 μg/μl DNase I with continuous and gentle agitation on a rotating shaker ...