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Citations for Becton, Dickinson and Company :
6901 - 6950 of 7237 citations for Dectin 1 CLEC7A Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... DAPI was added to mark the dead cells (1/2000) and the cells were sorted using the FACS Aria I cytometer (BD Biosciences). Twenty thousand Epcam-positive and Tomato-negative (PlusMinus ...
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bioRxiv - Developmental Biology 2024Quote: All cell culture of all species was maintained at 37°C with 5% CO2 and 5% O2 on Matrigel (1:100, BD Corning) coated plates unless otherwise specified ...
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bioRxiv - Immunology 2023Quote: ... cells were counted then stained with optimal concentrations of the following fluorophore-antibody conjugations: BB700 anti-IgG1 (clone A85-1, BD Biosciences), BB700 anti-IgG2a (clone R2-40 ...
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bioRxiv - Microbiology 2024Quote: ... xanthus cells were grown in liquid CYE medium (10 mM MOPS pH 7.6, 1% (w/v) Bacto™ casitone (BD Biosciences), 0.5% yeast extract and 8 mM MgSO4 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once with FACS buffer and fixed with 1% paraformaldehyde for 30 minutes prior to analysis on an LSRII flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... diluted 1:10 in DI H2O for 10 minutes within 1 hour of sample collection and then washed twice with Cell Staining Buffer (BD Biosciences). For cell surface staining ...
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bioRxiv - Cell Biology 2024Quote: ... organoids were dissociated and mCherry positive cells (marking successful electroporation of the pCAS9-mCherry-Frame+1 plasmid) were sorted on ARIAIII sorter (BD Biosciences) and seeded in Matrigel with normal organoid media supplemented with rock inhibitor Y-27632 (10 μM) ...
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bioRxiv - Cancer Biology 2023Quote: ... per well of U-bottom plate and coincubated for 4h at 37°C in the presence of NH4Cl and 1 µl Golgi PLUG (BD Biosciences). After that time cells were washed three times with 200 µl PBS and centrifuged (5 min ...
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1× perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Immunology 2023Quote: ... Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution, clone R2-40, BD Bioscience) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted by their distinct proliferation peaks (peak 0, peak 1 and peak 2) on FACS-Aria and Influx Cell Sorters (BD Biosciences). Cells were kept on ice and after washing with cold PBS were immediately resuspended in Trizol for RNA extraction ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor or normal organ tissue slides were incubated with primary antibody of Ki-67 (1:10000 dilution; Cat# 550609, BD Biosciences), p-Abi-1-S323 (1:500 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Gates were determined using the isotype controls mouse anti-IgG2b κ (APC-conjugated, Clone 27-35, 1:5, BD Biosciences, US) and mouse anti-IgG2a κ (PE-Cy7-conjugated ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with conjugated antibodies for 1 hour on ice: mouse anti-C-peptide antibody conjugated with Alexa Fluor 647 (BD Biosciences) and mouse anti-glucagon antibody conjugated with Bv421 (BD Biosciences) ...
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bioRxiv - Biophysics 2022Quote: ... samples were first resuspended in 15.5 μL PBS/0.1% adBSA and incubated with 0.5 μg of rat anti-mouse CD9-PE (Clone: KMC8, IgG2a, κ lot 7268877, BD Biosciences) or matched isotype antibodies (Rat IgG2a ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed and resuspended in 1% BSA with DAPI and detected by flow cytometry on a BD LSRFORTESSA X-20 (BD Biosciences). Data analysis was done using FlowJo V10.
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...
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bioRxiv - Systems Biology 2023Quote: ... all transformants were cultured individually in 96-well plates in 1 ml of the appropriate minimal liquid media (AD: -Leu; BD: -Trp) at 30°C until saturated (∼36-48 hours) ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were resuspended with 500 μl PBS containing 1% BSA for detection in BD Accuri C6 flow cytometer system (BD Biosciences) at excitation 488 nm and emission 519 nm ...
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bioRxiv - Immunology 2023Quote: ... Bacteria were subcultured from the chocolate agar into brain heart infusion (BHI) broth supplemented with 1% BBL IsoVitaleX (BD Biosciences, # 211875) to a starting OD600=0.1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 x 106 dissociated organoid cells in 100 µl hepatocyte culture medium and 5% Matrigel were injected subcutaneously into the flank using a 1 ml syringe with 25G needle (BD 305122). For human grafts ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed in 3ml FACS buffer before being fixed in 1% paraformaldehyde and analyzed within 5 days using a Fortessa Flow Cytometer and BD FACSDiva Software (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... NTHi was inoculated into 50 ml brain-heart-infusion broth (Roth, Germany) with 1% supplement B (BD Difco™; BD, Germany) and incubated overnight at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and CD4+FYP+ Treg cells at indicated Treg : Tresp ratios in the presence of 1 μg/ml anti-CD3 antibody (BD Pharmingen). After 3 days ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 300 µg/mL DNase for 1 hour at 37°C to expose incorporated brdU and then stained with an APC anti-brdU antibody (1:50, BD Biosciences). 1X BD Perm/Wash in ddH2O was used as a staining and washing buffer ...
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bioRxiv - Immunology 2022Quote: ... GFP expression in peripheral blood B and T cells was screened using anti-CD19-PE (BD, clone 1D3, dilution 1/100), anti-CD3e-PerCP-Cy5.5 (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in staining buffer consisting of 50% PBS containing 1% bovine serum albumin (BSA) and 50% Brilliant Stain Buffer (BD Biosciences) and incubated for 20 minutes at 4°C in the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μl of this suspension (1×10e5) was transferred to a 5 ml culture tube and 5 μl of FITC Annexin V (BD Biosciences) added ...
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bioRxiv - Biophysics 2022Quote: ... Saccharomyces cerevisiae strain W303-GAL4 was grown overnight in YPDA medium (yeast extract 1 %, adenine sulfate 540 μM, peptone 2 % (BD Biosciences), glucose 2 % ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then the resulting 350 μL volume was drawn twice into a 1 mL luer slip tip tuberculin syringe (BD Catalog # 309659) fitted with a 27G-½” needle ...
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bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.
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bioRxiv - Developmental Biology 2023Quote: ... and then resuspended in 100 μL Annexin V binding buffer with either (i) FITC-Annexin V conjugated antibody (1:33 dilution in TBST) and BD propidium iodide staining solution (BD Pharmingen) or (ii ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions of ex vivo organs were co-cultured with DCs in complete RMPI at a ratio of approximately 10 T cells to 1 DC in the presence of Golgi Plug (BD Bioscience). Co-cultures were incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... HC-04 cells were washed and fixed with 1% PFA in PBS before quantifying dextran-positive cells by flow cytometry (LSR II instrument, BD Biosciences). For data analysis using FlowJo V.10.0.8 (Tree Star) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated for 45 min at 4°C with rocking with 1:250 of each of the following antibodies: Negative selection markers: BV421 Rat anti-mouse anti-CD31 (562939, BD Biosciences), and BV421 Rat anti-mouse CD45 (563890 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD49fhighEpCAMlowLin- cells from 2-month-old C57BL/6 WT mice were sorted using FACS and resuspended in culture media (DMEM/F12+2% FBS+1% PSA+2% B27) supplemented with EGF (10 ng/ mL, BD Bioscience), Rspo1(250 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... CD49fhighEpCAMlowLin- cells were collected from FACS and cultured in 200 μL culture media (DMEM/F12+2% FBS+1% PSA+2% B27) supplemented with 10 ng/ mL EGF (BD Bioscience), 250 ng/ mL Rspo1(R&D) ...
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bioRxiv - Cancer Biology 2023Quote: ... mice bone marrow mononuclear cells were first stained with lineage (Lin) cocktail containing biotinylated antibodies against Ly-6G/Ly-6C (Gr-1) Biotin RB6-8C5 (BD Bioscience) (1:100) ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed with 2% paraformaldehyde for 20 min and resuspended in 350 ul 1% BSA/PBS for phenotyping by FACSDivacytometer (BD Biosciences). To set up the compensation control ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then resuspended in BD Perm/wash buffer with the following antibodies: BV650 IL4 (1:50, BD Bioscience, cat#564004), APC-Fire750 IFNγ (1:80 ...
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bioRxiv - Neuroscience 2023Quote: ... Stained cells were analyzed and 7AAD−Lin−Vcam−Sca1+ (Stem cell antigen 1; Ly6a) (FAPs) were isolated using FACS Aria III cell sorter (BD Biosciences) with 4-way-purity precision ...
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bioRxiv - Cell Biology 2023Quote: ... NTHi was inoculated into 50 ml brain-heart-infusion broth (Roth, Germany) with 1% supplement B (BD Difco™; BD, Germany) and incubated overnight at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... iPSC were detached and singularised using EDTA solution (0.5 M EDTA/PBS) and replated on Matrigel (1:120, BD Bioscience, #354230) at a density of 8,000-12,000 cells/cm2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and total DNA (7-AAD 1:50 solution dilution) and analyzed by flow cytometer in a BD LSRFortessa cell analyzer (BD Biosciences) using 488 nm and 640 nm lasers ...
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bioRxiv - Cancer Biology 2023Quote: ... After washing the slides were blocked for 30 min in Superblock solution following the incubation with the primary antibodies mouse anti-BrdU/IdU (1:100, BD Bioscscience) and rat anti-BRDU/CIdU (1:200 ...