Labshake search
Citations for Becton, Dickinson and Company :
6851 - 6900 of 7237 citations for Dectin 1 CLEC7A Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were fixed in 1% formalin and analyzed on a BD FACS Canto II running the FACS Diva Software (BD Biosciences). Fluorescence values were normalized to an unstained control samples and initial compensation applied using IgG-APC control antibody (Miltenyi Biotech ...
-
bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
-
bioRxiv - Microbiology 2020Quote: ... albicans MTL a/α clinical isolate SC5314 72 was used for all experiments and was routinely grown in YPD (1% yeast extract, 2% peptone, 2% glucose; all BD Biosciences) at 30 °C.
-
bioRxiv - Immunology 2021Quote: ... stained cells were resuspended in 1% paraformaldehyde (PFA) and acquired using Stained cells were acquired on a BD LSRFortessa (BD Biosciences) and analysis performed using FlowJo v10.0.8 (Tree Star ...
-
bioRxiv - Immunology 2021Quote: ... and cell surface markers were stained with the following anti-mouse antibodies at 1:200 dilution per 106 cells: Ly6C (AL-21; BD Biosciences), Ly6G (1A8 ...
-
bioRxiv - Immunology 2020Quote: ... spleen or lymph node cells in complete RMPI at a ratio of approximately 10 T cells to 1 DC in the presence of Golgi Plug (BD Bioscience). Co-cultures were incubated at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... Sections were stained using the following antibodies: rat anti-mouse Ly-6C/Ly-6G (RB6-8C5, BD Bioscience, #550291, 1/200) followed by AlexaFluor647-conjugated goat anti-rat IgG (ThermoFisher Scientific ...
-
bioRxiv - Bioengineering 2021Quote: ... To express CRE and induce recombination phleomycin resistant colonies were selected and grown overnight in 3ml of YP-Raffinose (1% w/v yeast extract (BD Biosciences), 2% w/v Bacto-peptone (BD Biosciences) ...
-
bioRxiv - Immunology 2020Quote: ... splenocytes and tumor-infiltrating cells were cocultured with 1 μmol/L of AdpgkMut or AH1 peptide in the presence of Brefeldin A (BD Biosciences) for 5h in vitro ...
-
bioRxiv - Immunology 2020Quote: For data acquisition on the BD FACSymphony the following amounts of 14 different fluorescent streptavidin conjugates were added to 10 μl of pMHC monomer (100 μg/ml): 1 μl of SA-BB790 (BD, custom), 1 μl of SA-BB630 (BD ...
-
bioRxiv - Immunology 2021Quote: ... Resting- or in vitro-generated effector CD4+ or CD8+ T cells (105 cells/well) were stimulated with 1 μg/ml plate-bound anti-mouse CD3 mAb (BD Bioscience) in combination with different concentrations of P3CSK4 ...
-
bioRxiv - Microbiology 2021Quote: ... Escherichia coli strains were routinely grown aerobically at 37 °C under aerobic conditions with shaking at 1 × g in Luria-Bertani medium (BD, USA), supplemented with 25 μg mL−1 chloramphenicol (Merck ...
-
bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
-
bioRxiv - Immunology 2021Quote: ... The cells were washed two times and resuspended in 1% paraformaldehyde/1x PBS for collection on a LSR II or FACSymphony flow cytometer (BD Biosciences). Data was analyzed in Flow Jo version 9.9.4.
-
bioRxiv - Cancer Biology 2020Quote: Cells either from culture or from transwell assay were washed in PBS and incubated in 2% FBS in PBS with anti-CCR7-FITC (1:25, #561271, BD Biosciences) and anti-ROR1-APC (1:25 ...
-
bioRxiv - Neuroscience 2022Quote: ... the cell suspension was then incubated on two PDGFRα plates coated with rat anti-PDGFRα IgG (BD Biosciences, 558774; 1:500). The adherent cells were washed with PBS to remove all antigen-negative nonadherent cells ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were fixed with IC fix (eBioscience88-8824-00) for 30 minutes followed by incubation with CD74 FITC 1:100 (BD 561941) in permeabilization buffer (eBioscience88-8824-00 ...
-
bioRxiv - Cell Biology 2022Quote: Freshly dissected LW whole mount preparations from 6 wild-type and 4 Lrp2-/267 mice were incubated with rat anti-CD24 conjugated with Phycoerythrin (PE; 1:100; BD Pharmingen) in Neurobasal medium (Gibco ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then washed twice with 1× PBS and flow cytometry analysis was performed on a FACS Canto II flow cytometer (BD Biosciences); data analysis was carried out using FlowJo software (BD Biosciences).
-
bioRxiv - Immunology 2022Quote: ... were re-stimulated in vitro for 5h at 37°C 5% CO2 in cRPMI supplemented with GolgiStop (1/250; BD bioscience), GolgiPlug (1/500 ...
-
bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
-
bioRxiv - Immunology 2022Quote: ... was added and 0.6-1×106 lymphocyte-gated events were immediately acquired on an LSRII SORP (BD, San Diego, CA, USA).
-
bioRxiv - Immunology 2022Quote: ... ELISAs were performed using paired rat anti-mouse IgE antibodies (capture with 1 μg/mL purified R35-72, BD Biosciences, detect with biotinylated R35-118, BD Biosciences) and streptavidin-HRP (BD Biosciences) ...
-
bioRxiv - Immunology 2022Quote: ... and others were fixed using PBS containing 1% paraformaldehyde (Labsynth, Diadema, SP, Brazil) and acquired in FACS Canto II (BD Biosciences). Analyses were performed in FlowJo software (Becton Dickinson and Company).
-
bioRxiv - Microbiology 2022Quote: ... xanthus cells were grown in liquid CYE medium (10 mM MOPS pH 7.6, 1% (w/v) Bacto™ casitone (BD Biosciences), 0.5% yeast extract and 8 mM MgSO4 ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylated antibody bound to RBD in the presence of competitor was stained with Streptavidin-PE at a 1:1000 dilution (BD Biosciences) and analysed on a 200 Bioplex instruments ...
-
bioRxiv - Microbiology 2022Quote: ... 50 μL of DEs were diluted into 500 μL of FACS diluent buffer (PBS + 1% Tween-20) in a 5 mL 12 × 75 mm round bottom FACS tube (BD Biosciences). After standard autocalibration on the Sony SH800 ...
-
bioRxiv - Neuroscience 2022Quote: ... The resulting pellet was resuspended in 1 ml of complete neurosphere medium containing epidermal growth factor (EGF, 20 ng/ml; receptor grade, BD Biosciences) and basic fibroblast growth factor (bFGF ...
-
bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
-
bioRxiv - Immunology 2022Quote: ... cells were stained for p-STAT1 (clone 4alpha) or p-STAT3 (clone 4P/STAT3) for 1 hour (all from BD Biosciences) and were acquired on FACS LSRFortessa flow cytometer (BD Biosciences).
-
bioRxiv - Immunology 2022Quote: ... Data acquisition and analysis: 0.5-1×106 events were collected on a BD LSR Fortessa X-50 flow cytometer (BD Immunocytometry Systems). Electronic compensation was performed with antibody capture beads (BD Biosciences) ...
-
bioRxiv - Physiology 2022Quote: ... Novus) and F(ab’)2-donkey anti-Rabbit IgG (H+L) secondary antibody PE (1:100, 12-4739-81, BD bioscience) at 4℃ for 1h ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were submitted to the same procedure as for flow cytometry analysis but without 1% PFA treatment and processed using BD FACSAria (BD Biosciences).
-
bioRxiv - Molecular Biology 2022Quote: ... Confluent definitive endoderm cells are then passed 1:3 in presence of Rock Inhibitor on growth factors reduced Matrigel (BD Biosciences) and cultured for 8 days in differentiation medium (DMEM F12 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tumors were engrafted in NSG mice by subcutaneous injection of 1 × 106 cells of H2081 in RPMI1640 medium supplemented with 50% Matrigel (BD Biosciences). Ten days after the injection ...
-
bioRxiv - Biochemistry 2022Quote: ... The cells were stained with anti-HbF APC antibody (dilution 1:50) and was acquired and analysed using Cytoflex LX Flow Cytometer (Beckmann Coulter) or AriaIII flow cytometer (BD Biosciences) and analysed using FlowJo (BD Biosciences) ...
-
bioRxiv - Immunology 2022Quote: ... Then live Tetr-gag+Pent-gag+CD3+CD8+ cells were sorted by flow cytometry into PBS buffer 1% BSA 2 mM EDTA using a FACSAria III (BD Biosciences) equipped with 488 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Primordial germ cells were purified based on the expression of Zombie Red−/SSEA-1+/CD61+ using BD FACS Aria II Cell Sorter (BD Bioscience). Somatic gonad cells were purified based on the expression of Zombie Red−/SSEA-1−/CD61 ...
-
bioRxiv - Microbiology 2022Quote: ... or the TAPI (prepared as described above) was administered directly into the pocket via a 1 mL syringe (BD, cat# 309628). The implants ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 % bovine serum albumin (BSA, Jackson, cat. no. 001-000-162) and 10% Perm/Wash buffer (BD Biosciences, cat. no. 554714) at RT for 1 hour ...
-
bioRxiv - Immunology 2024Quote: ... with phorbol 12-myristate 13-acetate (PMA, 20 ng/ml) plus ionomycin (1 mg/ml) in the presence of GolgiPlug/GolgiStop (BD Biosciences). Fluorescence was quantified using an LSR-II or Fortessa X20 flow cytometer (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... Cell purity was determined by flow cytometry (see Figure S6A) using antibodies specific against CD20-FITC (1:100 345792, BD Biosciences), CD14-PerCP (1:50 ...
-
bioRxiv - Microbiology 2024Quote: ... Sputum samples were diluted in 1% proteose peptone and then plated on oxidation/fermentation-polymyxin-bacitracin-lactose (OFPBL) media (BD Biosciences) to select single B ...
-
bioRxiv - Genomics 2024Quote: ... cells were resuspended in 110 µl of FACS Buffer supplemented with 1 µM Propidium Iodide and analyzed on a FACS Calibur Flow Cytometer (BD Biosciences).
-
bioRxiv - Microbiology 2024Quote: ... Cells were stained with rabbit anti-GRA7 (1:5000) (gift from John Boothroyd, Stanford) and rat anti-LAMP1 (1D4B, BD Biosciences) (1:500 ...
-
bioRxiv - Microbiology 2024Quote: ... and then incubated overnight with hamster anti-CD3ε antibody diluted in 1% goat serum/0.3% Triton-X100/TBS (BD Biosciences, 550277). The next day samples were washed in TBS and incubated at room temperature in goat anti-hamster 647 (Life Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were pelleted and washed twice with staining buffer and suspend 1 ml of staining buffer with DAPI for an end concentration of 1 x 106 cells/ml and DAPI (0.2 ug/ml; BD Biosciences, 564907) added ...
-
bioRxiv - Microbiology 2023Quote: ... pH 7.2) or BS-N buffer supplemented with 1% casein hydrolysate (casamino acids technical grade; Becton, Dickinson and Company, NJ, USA) at an OD660 of 4.0 was transferred to a ProteoSave SS 1.5-mL centrifuge tube (Sumitomo Bakelite ...
-
bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
-
bioRxiv - Cell Biology 2024Quote: ... and screened for protein levels via Western blots with monoclonal mouse anti-IQGAP1 (1:1000; 610612, BD Biosciences, East Rutherford, NJ), polyclonal rabbit α-tubulin (1:2500 ...