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Citations for Becton, Dickinson and Company :
6601 - 6650 of 6817 citations for 1 Hydroxymethyl benzo cd indol 2 1H one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Cells were then immuno-stained 1 hour at room temperature with a panel of antibodies: anti-CD3 (563725, BD, Franklin Lakes, NJ), anti-TNFα (Biolegend 502938) ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-CD28 antibodies (eBioscience, clone 37.51, 1 µg/ml) on TC plates pre-coated with anti-hamster antibody (BD Pharmingen, G94-56), and IL-2 (Immunotools ...
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bioRxiv - Cell Biology 2024Quote: ... cells were counted using a hemacytometer and then treated with an Fc blocking buffer containing anti-Cd16/32 (BD Biosciences, 1:500) for 20 minutes at 4 degrees Celsius before a 30 minute incubation with antibody master mixes for the myeloid or lymphoid panels at 4 degrees Celsius ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD, 354277; Fisher Scientific, 140675). For the first 24 hours after passaging ...
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bioRxiv - Immunology 2023Quote: ... and stained for the following surface markers on ice for 30 min: CD4 (APC-Cy7, 1:100, clone RM4-5, BD Biosciences, 565650), CD8b (Alexa Fluor 488 ...
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bioRxiv - Bioengineering 2024Quote: ... 3-4×105 B16F10-ZsGreen cells were implanted subcutaneously (s.c.) into the right flank of mice after shaving through a 25G x 1 ½ in needle (BD PrecisionGlide Needle #305127). For the growth control and survival study ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then stained at a 1:50 dilution with antibodies to immune cell targets as follows: CD3- FITC (BD Biosciences, 555332), CD8-PE (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and resuspended in flow buffer containing 1% FA to be analyzed using the LSR Fortessa (BD Bioscience, USA). Data were analyzed using FlowJo 10 software (FlowJo LLC ...
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bioRxiv - Cell Biology 2022Quote: ... Pelleted cells were incubated with anti-GFP-AF647 antibody (1:150, Alexa Fluor 647 Mouse Anti-GFP Clone 1A12-6-18, BD Biosciences 565197) or rabbit anti-ITGA5 (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... using a 23 G needle and a 1 ml syringe.Blood was collected into a serum separator tube (365967, BD, Franklin Lakes NJ), allowed to clot at room temperature for at least 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: The following antibodies were obtained commercially and used at the indicated dilutions for immunofluorescence: mouse monoclonal TOM20 (BD Biosciences #612278, 1:1000), TOMM20 (Abcam #ab186734 ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Solution was washed 3x with FACS buffer following same centrifugation as above and then resuspended in 500 uL of FACS buffer and 1 uL DAPI (BD Biosciences, 564907) and filtered into filter-top FACS tubes ...
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bioRxiv - Immunology 2022Quote: ... Solution was washed 3X with FACS buffer following same centrifugation as above and then resuspended in 500 uL of FACS buffer and 1 uL DAPI (BD Biosciences, 564907) and filtered into filter-top FACS tubes ...
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bioRxiv - Microbiology 2022Quote: ... gonorrhoeae testing contains 3.6% GC agar base (Oxoid™ GC agar # CM0367) and 1% defined growth supplement (BD BBL™ IsoVitaleX # 211876). Compound solutions described above were incorporated into GC agar medium to pour rectangular plates that represent 2-fold serial dilutions consisting of 12 compound dilution steps of test or control article ...
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bioRxiv - Physiology 2022Quote: ... Venous peripheral blood (4-6 ml for adults, 1- 3 mL for children and infants) was collected on-site on EDTA (BD vacutainer©) and immediately stored at 4-8°C until use ...
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bioRxiv - Neuroscience 2023Quote: ... for 10 min at 37 °C and 5 % CO2 before they were stained with Fixable Viability Stain 510 (1:100, #564406, BD Horizon, USA) for 15 min at room temperature (RT ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
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bioRxiv - Synthetic Biology 2023Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... were subcutaneously (s.c.) implanted into the right flank of mice after shaving through a 25G x 1 ½ in needle (BD PrecisionGlide Needle #305127). Mice were housed on a 12-hour/12-hour light/dark cycle and supplied food ad libitum ...
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bioRxiv - Immunology 2022Quote: ... The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA) with the following antibodies/reagents (all 1:200 diluted) for 30 min on ice: anti-CD20-PE-Cy7 (BD Biosciences, 335828), anti-CD14-APC-eFluor 780 (Thermo Fischer Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... the cells were stained with Hoechst live/dead staining (1:10000) before flow-cytometry and recorded using a FACSymphony™ (BD Biosciences) cytometer.
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bioRxiv - Immunology 2023Quote: ... Spleens were harvested and single-cell suspensions were stained with B220 conjugated to fluorescein isothiocyanate (B220-FITC, BD Biosciences, 1:500 dilution), Fas conjugated to PE-cyanine 7 (Fas-PE-Cy7 ...
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bioRxiv - Genomics 2023Quote: Trypsinized or MACS sorted cells were incubated at 4°C for 20 min with APC-conjugated anti-CD56 antibodies (1:40, BD Pharmingen #555518) or isotype control (1:40 ...
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bioRxiv - Immunology 2022Quote: ... with the following antibodies/reagents (all at 1:200 dilution) for 30 min on ice: anti-CD20-PE-Cy7 (BD Biosciences, 335828), anti-CD14-APC-eFluor 780 (Thermo Fischer Scientific ...
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bioRxiv - Genetics 2023Quote: Fluorescently conjugated antibodies for cell surface staining (Supplementary Table 1) were prepared in Brilliant Stain Buffer (Cat. no. 556349, BD Biosciences, Australia) and incubated with cells in a 96 well plate (Cat ...
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bioRxiv - Immunology 2024Quote: ... samples were washed with 1X PBS and fixed in 1% formaldehyde before acquisition on a FACSCanto™ II (Beckmann Coulter) using the FACS DiVa software (BD, Biosciences). Also ...
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bioRxiv - Immunology 2024Quote: ... Stained samples were fixed in 1% formaldehyde and acquired on FACSCanto™ II (Beckmann Coulter) using the FACS DiVa software (BD, Biosciences). Data were analysed using FlowJo v10.6 (Tree Star Inc) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: We performed extracellular staining using the following antibodies (dilution, clone, company, catalog number): anti-mouse CD45.2 BUV395 (1:100, 104, BD Biosciences, Cat. 564616), anti-mouse CD3 BUV737 (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... the stromal vascular fraction was sorted into 96-well plate at 1 cell per well using BD FACSAria II Cell Sorter (BD Biosciences, USA). The cells were spontaneously immortalized by passaging for more than 30 passages ...
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bioRxiv - Immunology 2024Quote: ... Samples were then spun down at 300 × g for 5 minutes at 4°C and resuspended in full antibody cocktail containing fluorophore-conjugated antibodies (Table 1) and FC block in BD Horizon Brilliant Stain Buffer (BD Biosciences, #563794). Cells were incubated with antibodies for 20 minutes at 4°C before three washes in FACS buffer containing 0.25mM EDTA ...
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bioRxiv - Immunology 2024Quote: ... Samples were washed 3x after staining and resuspended in 1% BSA for analysis on an a for analysis on an LSRFortessa flow cytometer (BD Life Sciences).
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bioRxiv - Bioengineering 2024Quote: The expression of the exhaustion markers PD-1 and TIM-3 was evaluated using anti-PD1 (cat. 560795, BD Biosciences, BD PharMingen) and anti-TIM3 (cat ...
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bioRxiv - Systems Biology 2024Quote: ... and lawns of pAWP78-P89-20ZRfae in E.coli S17-1 (overnight) were prepared for mating on plates with Hypho media supplemented with 5% Nutrient Broth (NB Difco, BD Life Sciences). Plates were incubated for 1 day at 30° C ...
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bioRxiv - Immunology 2024Quote: ... A cocktail of the surface stain (Supplemental Table 1) supplemented with BD Horizon™ Brilliant Stain buffer (BD Biosciences, Cat no. 566349) was added to each well (50 μL/well) ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed/permed with Foxp3 Transcription Factor Fixation/permeabilisation (eBiosciences, RELMα, iNOS, Ym-1) or IC Fixation/permeabilisation buffer (BD, intracellular cytokines) with paired perm/wash buffers ...
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bioRxiv - Immunology 2024Quote: ... The interphase was then re-suspended in FACS buffer and blocked with the Fc-blocker CD16/CD32 (553141, BD Biosciences, 1:100) for 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated with anti-mouse CD32 for 5 min at 4°C to block Fc receptors and stained with fluorochrome conjugated antibodies to CD45R/B220 PE (1:200) (clone RA3-6B2, BD Bioscience #553089), Ter119 PE (1:200 ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then incubated for 1 h with primary antibodies directed against the cis-Golgi marker GM130 (mouse anti-GM130, BD bioscience, 610822) and/or trans-Golgi marker TGN46 (sheep anti-TGN46 ...
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bioRxiv - Microbiology 2024Quote: ... The urine samples were obtained in sterile BD Vacutainer® urine C&S Preservative Tubes (BD, 1 Becton Drive, Franklin Lakes. NJ). Specifically ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked with 5% skimmed milk in TBS-Tween and incubated with anti-αSyn clone 42 (BD Biosciences, 1:1,000 dilution). In a slot blot ...
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bioRxiv - Immunology 2024Quote: ... IL-6 or TNF levels were measured in supernatants from iPSC-macrophages or THP-1 cells using the IP-10 (BD Biosciences, #550926), IL-6 (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1 ml of whole blood was collected from each mouse by retro-orbital extraction into lithium-heparin tubes (BD Medical, #368496) containing 1.5 ml pre-warmed culture medium lacking serum ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed with FACS staining buffer and incubated with α-BrdU antibody conjugated to APC (1:50, BD Pharmigen flow kit) and α-pHH3 (Ser10 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed twice with 1% FBS in PBS (staining buffer) before incubation with 2.5 μg Fc block (BD Biosciences Cat 564219) in staining buffer for 15 min at room temperature ...